不同品种彩色马蹄莲灭菌条件优化与不定芽诱导
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摘要
以‘穆拉诺’(Murano)、‘孟菲斯’(Memphis)、‘凯尔索’(Kelso)、‘刀锋’(Blade)、‘色拉达’(Salada) 5个品种彩色马蹄莲的块茎为试验材料,对其进行灭菌时间、灭菌方法及不定芽诱导条件的筛选。结果表明,在灭菌时间上,不同品种间存在显著性差异,‘穆拉诺’、‘凯尔索’、‘刀锋’的最佳灭菌时间是15 min,‘孟菲斯’、‘色拉达’的最佳灭菌时间是18 min;灭菌方法,5个品种均在45℃水浴1 h和杀菌剂500倍液处理4 h的条件下,灭菌效果最好,初代培养成活率分别达到50%和70%左右;不定芽诱导阶段,不同品种间存在显著性差异,‘穆拉诺’、‘孟菲斯’、‘凯尔索’、‘色拉达’的最适培养基为MS+2 mg/L 6-BA+0.1 mg/L NAA,‘刀锋’最适培养基为MS+3 mg/L 6-BA+0.1 mg/L NAA。
The tubers of five species of Zabtadeschia hybrida, including 'Murano', 'Memphis', 'Kelso', 'Blade' and'Salada', were used as test materials to select the sterilization time, sterilization method and the adventitious bud induction conditions. The results showed that for the time of sterilization, there were significant differences between different varieties. The optimal sterilization time of 'Murano', 'Kelso' and 'Blade' was 15 min, and the best sterilization time of 'Memphis' and 'Salada' was 18 min. For sterilization method, five varieties were all under the condition of 45℃ water bath for 1 h and 500 times of bactericide for 4 h, and the sterilization effect was the best that original culture survival rate reached 50% and 70%, respectively. At the adventitious bud induction phase, there was a significant difference between different varieties. The optimal culture medium for 'Murano', 'Memphis', 'Kelso', and'Salada' was MS +2 mg/L 6-BA +0.1 mg/L NAA, and the optimal culture medium for 'Blade' was MS +3 mg/L6-BA+0.1 mg/L NAA.
The tubers of five species of Zabtadeschia hybrida, including 'Murano', 'Memphis', 'Kelso', 'Blade' and'Salada', were used as test materials to select the sterilization time, sterilization method and the adventitious bud induction conditions. The results showed that for the time of sterilization, there were significant differences between different varieties. The optimal sterilization time of 'Murano', 'Kelso' and 'Blade' was 15 min, and the best sterilization time of 'Memphis' and 'Salada' was 18 min. For sterilization method, five varieties were all under the condition of 45℃ water bath for 1 h and 500 times of bactericide for 4 h, and the sterilization effect was the best that original culture survival rate reached 50% and 70%, respectively. At the adventitious bud induction phase, there was a significant difference between different varieties. The optimal culture medium for 'Murano', 'Memphis', 'Kelso', and'Salada' was MS +2 mg/L 6-BA +0.1 mg/L NAA, and the optimal culture medium for 'Blade' was MS +3 mg/L6-BA+0.1 mg/L NAA.
引文
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Lin F.,Li J.Y.,Huang D.M.,Xu Y.,Li Y.J.,Li Y.X.,Ding Z.L.,and Tang F.L.,2015,Study of tissue culture and plant regeneration of Aquilaria sinensis,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(6):1296-1299(林妃,李敬阳,黄东梅,许奕,李羽佳,李艳霞,丁哲利,唐粉玲,2015,白木香组织培养技术及植株再生的研究,基因组学与应用生物学,34(6):1296-1299)
Lu F.F.,Shao G.Y.,and Chen X.M.,2007,Study on tissue culture of Zantedeschia ellictiana,Redai Nongye Kexue(Tro-pical Agricultural Science&Technology),30(3):41-47(陆方方,邵果园,陈晓梅,2007,黄色马蹄莲的组织培养研究,热带农业科技,30(3):41-47)
Shu X.C.,Li N.W.,He S.L.,and Peng F.,2010,Study on the tissue culture and rapid propagation of Zantedeschia hybrida'FengYan',Anhui Nongye Kexue(Journal of Anhui Agricultural Sciences),38(26):14268-14269(束晓春,李乃伟,何树兰,彭峰,2010,彩色马蹄莲‘凤眼’组培快繁技术的研究,安徽农业科学,38(26):14268-14269)
Wang J.Z.,Gao W.,Gao X.H.,Feng Q.,Sun S.L.,Zhang M.Z.,and Yu T.Q.,2005,Tissue culture of coloured common callalily(Zantedeschla),Beijing Nongxueyuan Xuebao(Journal of Beijing Agricultural College),20(2):10-13(王进忠,高文,高遐虹,冯强,孙淑玲,张民照,于同泉,2005,粉色马蹄莲组织培养研究,北京农学院学报,20(2):10-13)
Gong X.Q.,Qu F.N.,You C.R.,Sun W.T.,and Wang M.,2008,Establishment of callus induction system from leaves in Zantedeschia,Yantai Daxue Xuebao(Journal of Yantai University(Natural Science and Engineering Edition)),21(3):221-225(龚雪琴,曲复宁,由翠荣,孙婉婷,王萌,2008,彩色马蹄莲叶片愈伤组织培养体系的建立,烟台大学学报(自然科学与工程版),21(3):221-225)
Lin F.,Li J.Y.,Huang D.M.,Xu Y.,Li Y.J.,Li Y.X.,Ding Z.L.,and Tang F.L.,2015,Study of tissue culture and plant regeneration of Aquilaria sinensis,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(6):1296-1299(林妃,李敬阳,黄东梅,许奕,李羽佳,李艳霞,丁哲利,唐粉玲,2015,白木香组织培养技术及植株再生的研究,基因组学与应用生物学,34(6):1296-1299)
Lu F.F.,Shao G.Y.,and Chen X.M.,2007,Study on tissue culture of Zantedeschia ellictiana,Redai Nongye Kexue(Tro-pical Agricultural Science&Technology),30(3):41-47(陆方方,邵果园,陈晓梅,2007,黄色马蹄莲的组织培养研究,热带农业科技,30(3):41-47)
Shu X.C.,Li N.W.,He S.L.,and Peng F.,2010,Study on the tissue culture and rapid propagation of Zantedeschia hybrida'FengYan',Anhui Nongye Kexue(Journal of Anhui Agricultural Sciences),38(26):14268-14269(束晓春,李乃伟,何树兰,彭峰,2010,彩色马蹄莲‘凤眼’组培快繁技术的研究,安徽农业科学,38(26):14268-14269)
Wang J.Z.,Gao W.,Gao X.H.,Feng Q.,Sun S.L.,Zhang M.Z.,and Yu T.Q.,2005,Tissue culture of coloured common callalily(Zantedeschla),Beijing Nongxueyuan Xuebao(Journal of Beijing Agricultural College),20(2):10-13(王进忠,高文,高遐虹,冯强,孙淑玲,张民照,于同泉,2005,粉色马蹄莲组织培养研究,北京农学院学报,20(2):10-13)