莪术油注射液对宫颈癌细胞株Hela增殖及Wnt/β-catenin信号通路的影响
|
摘要
目的:探讨莪术油注射液对宫颈癌细胞Hela增殖的抑制作用及潜在作用机制。方法:采用CCK-8法筛选莪术油最佳给药剂量,并设立对照组和莪术油处理高、中、低剂量组。CCK-8法检测莪术油对Hela细胞生长曲线的影响;流式细胞术检测莪术油对Hela细胞周期的影响;Western Blot试验检测莪术油对Hela细胞中Wnt3a、β-catenin、C-myc、CyclinD1蛋白表达的调节作用。结果:CCK-8试验表明,莪术油给药剂量越大,其对Hela细胞增殖的抑制作用越明显,以高剂量组为最优。流式细胞术试验表明,莪术油可将Hela细胞阻滞在G1期,使S期和G2期细胞比例明显降低。Western Blot试验结果显示,莪术油处理后Wnt3a、β-catenin、C-myc、CyclinD1蛋白表达相较于对照组其表达明显降低。结论:莪术油注射液通过调节Wnt/β-catenin信号通路抑制宫颈癌细胞增殖。
OBJECTIVE To investigate the inhibitory effect of zedoary turmeric oil injection on the proliferation of cervical cancer cell line Hela and its potential mechanism. METHODS The optimal dose of zedoary turmeric oil was screened by CCK-8 method, and the control group and zedoary turmeric oil were used to treat high, medium and low dose groups. CCK-8 assay was used to detect the effect of zedoary turmeric oil on the growth curve of HeLa cells; flow cytometry was used to detect the effect of zedoary turmeric oil on the cell cycle of HeLa cells; Western blot assay was used to detect the regulatory effect of zedoary turmeric oil on the expression of Wnt3 a, β-catenin, C-myc, and CyclinD1 protein in HeLa cells.. RESULTS The CCK-8 test showed that higher dose of zedoary turmeric oil showed more inhibitory effect on Hela cell proliferation with high dose group being the best. Flow cytometry assay showed that zedoary turmeric oil could arrest HeLa cells in G1 phase, which significantly reduced the proportion of cells in S phase and G2 phase. Western Blot test results showed that the expression of Wnt3 a, β-catenin, C-myc, and CyclinD1 protein was significantly reduced after treatment with zedoary turmeric oil compared with the control group. CONCLUSION Zedoary turmeric oil injection can inhibit the proliferation of cervical cancer cells by regulating Wnt/β-catenin signaling pathway.
OBJECTIVE To investigate the inhibitory effect of zedoary turmeric oil injection on the proliferation of cervical cancer cell line Hela and its potential mechanism. METHODS The optimal dose of zedoary turmeric oil was screened by CCK-8 method, and the control group and zedoary turmeric oil were used to treat high, medium and low dose groups. CCK-8 assay was used to detect the effect of zedoary turmeric oil on the growth curve of HeLa cells; flow cytometry was used to detect the effect of zedoary turmeric oil on the cell cycle of HeLa cells; Western blot assay was used to detect the regulatory effect of zedoary turmeric oil on the expression of Wnt3 a, β-catenin, C-myc, and CyclinD1 protein in HeLa cells.. RESULTS The CCK-8 test showed that higher dose of zedoary turmeric oil showed more inhibitory effect on Hela cell proliferation with high dose group being the best. Flow cytometry assay showed that zedoary turmeric oil could arrest HeLa cells in G1 phase, which significantly reduced the proportion of cells in S phase and G2 phase. Western Blot test results showed that the expression of Wnt3 a, β-catenin, C-myc, and CyclinD1 protein was significantly reduced after treatment with zedoary turmeric oil compared with the control group. CONCLUSION Zedoary turmeric oil injection can inhibit the proliferation of cervical cancer cells by regulating Wnt/β-catenin signaling pathway.
引文
[1] Schoell WM,Janicek MF,Mirhashemi R. Epidemiology and biology of cervical cancer[J]. Seminars Surg Oncol,1999,16(3):203-211.
[2] Jansen KU. Toward vaccines against cervical cancer[J]. Curr Opin Drug Discov Devel,2004,7(2):228-232.
[3] Zhang NQ,Gao YN,Yan X,et al. Efficacy and safety of postoperative adjuvant chemotherapy for IB1-IIA cervical cancer with recurrent risk factors[J]. J Pract Obstet Gynecol(实用妇产科杂志),2014,30(01):21-25.
[4] Song AL,Xu ZG. Effects of zedoary turmeric oil on expression of VEGF mRNA in precancerous lesions of rat breast cancer[J].Chin J Tradit Chin Med(中华中医药学刊),2012,30(04):679-681.
[5] Li RX,Liu SJ,Kong DS,et al. Effects of zedoary turmeric oil on apoptosis of cisplatin-resistant ovarian cancer cells and its effect on STAT signaling pathway[J]. Chin Patent Med(中成药),2013,35(12):2570-2576.
[6] Wang JL,Wang X,Xia Q,et al. Study on the inhibitory effect of three sesquiterpenoids in zedoary turmeric oil on HepG2 cell proliferation[J]. Chin Patent Med(中成药),2014,36(07):1535-1539.
[7] Zhang WX,Luo J,Pan NS,et al. Effects of zedoary turmeric oil on coagulation function and expression of Bcl-2,Bax and Caspase-3 proteins in nude mice bearing cervical cancer[J]. Henan Tradit Chin Med(河南中医),2015,35(09):2051-2053.
[8] Tian L,Li WH.Ceramic oil injection induces apoptosis of human endometrial cancer cells (HEC-1-B) by up-regulating bax gene down-regulation of bcl-2 gene[J]. Everyone Health (Academic Edition)(大家健康(学术版)),2016,10(14):5.
[9] Shi W,Jiang L,Zhou YY,et al. Regulation of zedoary turmeric oil on telomerase activity and DNA damage response in breast cancer MCF-7 cells[J]. Tradit Chin Med Clin Pharm(中药新药与临床药理),2010,21(04):350-354.
[10] Yang MC, Fang G, Li L,et al. Effects of zedoary turmeric oil injection on the swelling and expression of Bcl-2 in human ovarian cancer SKOV3 cells[J]. Chine J Infor on Tradit Chin Med(中国中医药信息杂志),2009,16(10):34-35.
[11] Yang W,Chen DM,Fu W. Therapeutic effect of Baofukang suppository combined with interferon α-2b suppository on subclinical infection of cervical human papillomavirus[J].Hainan Med J(海南医学),2012,23(23):54-55.
[12] Chen R,Li WH. Effects of zedoary turmeric oil injection on proliferation and cell cycle of human endometrial cancer cells[J].World Health Inform Digest(世界最新医学信息文摘),2016,16(94):88+16.
[13] Guo MH,Song YP,Hao MF,et al. Effects of zedoary turmeric oil and carboplatin on proliferation of human ovarian cancer cell lines[J]. Chin Med Clin(中国药与临床),2016,16(07):1012-1014.
[2] Jansen KU. Toward vaccines against cervical cancer[J]. Curr Opin Drug Discov Devel,2004,7(2):228-232.
[3] Zhang NQ,Gao YN,Yan X,et al. Efficacy and safety of postoperative adjuvant chemotherapy for IB1-IIA cervical cancer with recurrent risk factors[J]. J Pract Obstet Gynecol(实用妇产科杂志),2014,30(01):21-25.
[4] Song AL,Xu ZG. Effects of zedoary turmeric oil on expression of VEGF mRNA in precancerous lesions of rat breast cancer[J].Chin J Tradit Chin Med(中华中医药学刊),2012,30(04):679-681.
[5] Li RX,Liu SJ,Kong DS,et al. Effects of zedoary turmeric oil on apoptosis of cisplatin-resistant ovarian cancer cells and its effect on STAT signaling pathway[J]. Chin Patent Med(中成药),2013,35(12):2570-2576.
[6] Wang JL,Wang X,Xia Q,et al. Study on the inhibitory effect of three sesquiterpenoids in zedoary turmeric oil on HepG2 cell proliferation[J]. Chin Patent Med(中成药),2014,36(07):1535-1539.
[7] Zhang WX,Luo J,Pan NS,et al. Effects of zedoary turmeric oil on coagulation function and expression of Bcl-2,Bax and Caspase-3 proteins in nude mice bearing cervical cancer[J]. Henan Tradit Chin Med(河南中医),2015,35(09):2051-2053.
[8] Tian L,Li WH.Ceramic oil injection induces apoptosis of human endometrial cancer cells (HEC-1-B) by up-regulating bax gene down-regulation of bcl-2 gene[J]. Everyone Health (Academic Edition)(大家健康(学术版)),2016,10(14):5.
[9] Shi W,Jiang L,Zhou YY,et al. Regulation of zedoary turmeric oil on telomerase activity and DNA damage response in breast cancer MCF-7 cells[J]. Tradit Chin Med Clin Pharm(中药新药与临床药理),2010,21(04):350-354.
[10] Yang MC, Fang G, Li L,et al. Effects of zedoary turmeric oil injection on the swelling and expression of Bcl-2 in human ovarian cancer SKOV3 cells[J]. Chine J Infor on Tradit Chin Med(中国中医药信息杂志),2009,16(10):34-35.
[11] Yang W,Chen DM,Fu W. Therapeutic effect of Baofukang suppository combined with interferon α-2b suppository on subclinical infection of cervical human papillomavirus[J].Hainan Med J(海南医学),2012,23(23):54-55.
[12] Chen R,Li WH. Effects of zedoary turmeric oil injection on proliferation and cell cycle of human endometrial cancer cells[J].World Health Inform Digest(世界最新医学信息文摘),2016,16(94):88+16.
[13] Guo MH,Song YP,Hao MF,et al. Effects of zedoary turmeric oil and carboplatin on proliferation of human ovarian cancer cell lines[J]. Chin Med Clin(中国药与临床),2016,16(07):1012-1014.