复合微生物杀菌剂对黄瓜灰霉病的防治
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摘要
[目的]对防治黄瓜灰霉病复合微生物杀菌剂进行研究。[方法]采用平板对峙法对菌株进行亲和性测定;通过测定微生物发酵滤液对灰霉病原菌孢子、菌丝等影响,确定最佳配比;盆栽试验确定制剂防病促生效果。[结果]菌株D1、BSD-2、ch2-22具有协同增效作用,发酵液以体积比5∶4∶1配比,活芽孢数为1.5×10~7 cfu/mL时,对黄瓜灰霉病防治效果达86.25%,并具有促生作用。[结论]复合微生物杀菌剂对黄瓜灰霉病具有较强防病促生作用,具有开发成新型生物农药的潜力。
[Aims] This paper aims to study the compound microbial disinfectant against cucumber gray mold.[Methods] The affinity test was carried out by plate confrontation method; The optimum ratio was determined by measuring the effect of strain fermentation on the spores and hyphae of gray mold pathogens. The pot test was used to determine the prophylactic effect of preparation. [Results] The strains D1, BSD-2, ch2-22 have synergistic effect.When the ratio of the three fermentation filtrates was 5 ∶4 ∶1 and the number of live spore was 1.5 ×10~7 cfu/m L, the compound fungicide for control of gray mold of cucumber was 86.25%. [Conclusions] Compound biocides have a strong anti-disease and promoting effect on botrytis cinerea, which has a potential to develop as a new type of biological pesticide.
[Aims] This paper aims to study the compound microbial disinfectant against cucumber gray mold.[Methods] The affinity test was carried out by plate confrontation method; The optimum ratio was determined by measuring the effect of strain fermentation on the spores and hyphae of gray mold pathogens. The pot test was used to determine the prophylactic effect of preparation. [Results] The strains D1, BSD-2, ch2-22 have synergistic effect.When the ratio of the three fermentation filtrates was 5 ∶4 ∶1 and the number of live spore was 1.5 ×10~7 cfu/m L, the compound fungicide for control of gray mold of cucumber was 86.25%. [Conclusions] Compound biocides have a strong anti-disease and promoting effect on botrytis cinerea, which has a potential to develop as a new type of biological pesticide.
引文
[1]李清华.温室黄瓜灰霉病的综合防控技术分析[J].农业科学,2015,35(18):13-14.
[2]仇艳肖,程辉彩,周竞,等.黄瓜灰霉病菌拮抗菌BBC-3的筛选、鉴定及抑菌特性[J].北方园艺,2016(6):84-88.
[3]陈源,卜元卿,单正军.微生物农药研究进展及各国管理现状[J].农药,2012,51(2):83-89.
[4]李丽梅,高丙利,柳东,等.抗黄瓜灰霉病的枯草芽孢杆菌筛选及其抑菌活性的初步研究[J].天津科技大学学报,2012,27(3):12-14.
[5]王晓辉,王贵鹏,张庆芳,等.一株抗灰霉病解淀粉芽孢杆菌的筛选鉴定及抑菌蛋白的分离[J].吉林农业科学,2015,40(1):64-67.
[6]黄蓉,黄盼,黄瑞荣,等.一株酵母菌的鉴定及其挥发性物质防病测定[J].江西农业大学学报,2015,37(5):903-908.
[7]徐大勇,李志栋,李峰,等.番茄灰霉病拮抗放线菌的筛选、鉴定及其活性评价[J].中国生物防治学报,2015,28(2):298-302.
[8]胡瑞平,张铎,张丽萍,等.枯草芽孢杆菌BSD-2一种抗菌肽的分离纯化与鉴定[J].华北农学报,2011,26(6):201-206.
[9]吕钊.生防解淀粉芽胞杆菌的分离鉴定及其作用研究(硕士论文)[D].石家庄:河北师范大学,2013.
[10]张蕾,张铎,张丽萍,等.枯草芽孢杆菌BSD-2产抗菌肽发酵培养基的优化[J].食品科学,2011,31(3):189-192.
[11]仇艳肖.黄瓜灰霉病高效拮抗菌的筛选鉴定及其作用研究(硕士论文)[D].石家庄:河北师范大学,2012.
[12]吴红玉,陈泽林,侯满芝.3%苦参碱水剂对黄瓜灰霉病的室内毒力测定和田间防治效果[J].山西农业大学学报(自然科学版),2015,35(6):630-633.
[13]SIDDIQUI Z A,ZEBRAA N.Biocontrol of Wilt Disease Complex of Pea Using Pseudomonas fluorescens and Rhizobium sp.[J].Archives of Phytopathology and Plant Protection,2012,45(19):2340-2346.
[14]葛红莲,张福丽,刘志华.混合菌剂PB12防治黄瓜疫病效果评价以及对土壤酶活性的影响[J].中国生物防治学报,2015,31(2):229-235.
[2]仇艳肖,程辉彩,周竞,等.黄瓜灰霉病菌拮抗菌BBC-3的筛选、鉴定及抑菌特性[J].北方园艺,2016(6):84-88.
[3]陈源,卜元卿,单正军.微生物农药研究进展及各国管理现状[J].农药,2012,51(2):83-89.
[4]李丽梅,高丙利,柳东,等.抗黄瓜灰霉病的枯草芽孢杆菌筛选及其抑菌活性的初步研究[J].天津科技大学学报,2012,27(3):12-14.
[5]王晓辉,王贵鹏,张庆芳,等.一株抗灰霉病解淀粉芽孢杆菌的筛选鉴定及抑菌蛋白的分离[J].吉林农业科学,2015,40(1):64-67.
[6]黄蓉,黄盼,黄瑞荣,等.一株酵母菌的鉴定及其挥发性物质防病测定[J].江西农业大学学报,2015,37(5):903-908.
[7]徐大勇,李志栋,李峰,等.番茄灰霉病拮抗放线菌的筛选、鉴定及其活性评价[J].中国生物防治学报,2015,28(2):298-302.
[8]胡瑞平,张铎,张丽萍,等.枯草芽孢杆菌BSD-2一种抗菌肽的分离纯化与鉴定[J].华北农学报,2011,26(6):201-206.
[9]吕钊.生防解淀粉芽胞杆菌的分离鉴定及其作用研究(硕士论文)[D].石家庄:河北师范大学,2013.
[10]张蕾,张铎,张丽萍,等.枯草芽孢杆菌BSD-2产抗菌肽发酵培养基的优化[J].食品科学,2011,31(3):189-192.
[11]仇艳肖.黄瓜灰霉病高效拮抗菌的筛选鉴定及其作用研究(硕士论文)[D].石家庄:河北师范大学,2012.
[12]吴红玉,陈泽林,侯满芝.3%苦参碱水剂对黄瓜灰霉病的室内毒力测定和田间防治效果[J].山西农业大学学报(自然科学版),2015,35(6):630-633.
[13]SIDDIQUI Z A,ZEBRAA N.Biocontrol of Wilt Disease Complex of Pea Using Pseudomonas fluorescens and Rhizobium sp.[J].Archives of Phytopathology and Plant Protection,2012,45(19):2340-2346.
[14]葛红莲,张福丽,刘志华.混合菌剂PB12防治黄瓜疫病效果评价以及对土壤酶活性的影响[J].中国生物防治学报,2015,31(2):229-235.