聚羟基丙烯酸在宫颈组织P16蛋白表达检测中的应用
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摘要
目的:观察聚羟基丙烯酸(环保固定液)与10%中性缓冲福尔马林(传统固定液)在免疫组化法检测宫颈组织P16蛋白表达的结果,探讨其替代10%中性缓冲福尔马林的可行性。方法:收集2015年3月—2017年11月于南方医科大学附属中山博爱医院门诊及妇科住院部送检的宫颈组织标本245例,其中包括正常及慢性宫颈炎组52例,低级别鳞状上皮内病变(LSIL)组87例,高级别鳞状上皮内病变(HSIL)组61例,宫颈癌组45例。同一病变部位取材2块,随机分2组,命名为A、B组。A组采用10%中性缓冲福尔马林固定制作切片245张;B组采用聚羟基丙烯酸固定制作切片245张。采用免疫组化法检测宫颈组织P16蛋白表达情况。结果:①生物显微镜下,A、B组切片均背景清晰、阳性着色定位准确、阳性强度可靠、呈色鲜明,无非特异性染色产生、阴性和阳性对照染色结果可靠。②慢性宫颈炎组、LSIL组、HSIL组及宫颈癌组的A组P16蛋白阳性率分别为5.77%、35.63%、80.33%和100.00%,慢性宫颈炎组、LSIL组、HSIL组及宫颈癌组的B组P16蛋白阳性率分别为5.77%、33.33%、81.97%和100.00%。A、B组间对应的各级宫颈病变,P16蛋白表达阳性率差异均无统计学意义(均P>0.05)。结论:用聚羟基丙烯酸替代10%中性缓冲福尔马林对免疫组化法检测宫颈组织P16蛋白表达阳性率无明显影响,聚羟基丙烯酸作为固定液在免疫组化法检测宫颈组织P16蛋白表达中有一定的使用价值。
Objective:To observe the expression of P16 protein in cervix tissue by immunohistochemical method and10% neutral buffer Faure Marin(traditional stationary liquid), and explore the feasibility of replacing it with 10% neutral buffer Faure Marin. Methods:245 specimens of cervical tissue were collected from the outpatient department and the department of gynaecologic inpatient department of Zhongshan BOAI Hospital Affiliated to Southern Medical University from March 2015 to November 2017. There were 52 cases of normal and chronic cervicitis, 87 cases in group LSIL, 61 in group HSIL and 45 in cervical cancer group. 2 pieces of the same lesion were collected and randomly divided into two groups, which were named group A and B. In group A, 245 sections were made by 10% neutral buffered formalin, and 245 sections were made by polyhydroxy acrylic acid in group B. Immunohistochemical method was used to detect the expression of P16 protein in cervical tissue. Results:①Under the biological microscope, the two groups of A and B slices had clear background, positive collocation,accurate location, strong positive intensity, bright and fresh color, and no specific staining, negative and positive staining results were reliable.② In group A, the positive rate of p16 protein in chronic cervicitis group, LSIL group, HSIL group and cervical cancer group were 5.77%, 35.63%, 80.33% and 100.00%, respectively. The positive rate of p16 protein in B group was 5.77%,33.33%, 81.97% and 100.00% respectively in chronic cervicitis group, LSIL group, HSIL group and cervical cancer group.There was no significant difference in the positive rate of P16 protein expression between A and B groups(P >0.05).Conclusions:Using polyhydroxyacrylic acid instead of 10% neutral buffer Faure Marin has no significant difference on the positive rate of P16 protein expression in cervical tissue by immunohistochemical method. Polyhydroxyacrylic acid as a stationary liquid has certain value in the detection of P16 protein expression in cervical tissue by immunohistochemical method.
Objective:To observe the expression of P16 protein in cervix tissue by immunohistochemical method and10% neutral buffer Faure Marin(traditional stationary liquid), and explore the feasibility of replacing it with 10% neutral buffer Faure Marin. Methods:245 specimens of cervical tissue were collected from the outpatient department and the department of gynaecologic inpatient department of Zhongshan BOAI Hospital Affiliated to Southern Medical University from March 2015 to November 2017. There were 52 cases of normal and chronic cervicitis, 87 cases in group LSIL, 61 in group HSIL and 45 in cervical cancer group. 2 pieces of the same lesion were collected and randomly divided into two groups, which were named group A and B. In group A, 245 sections were made by 10% neutral buffered formalin, and 245 sections were made by polyhydroxy acrylic acid in group B. Immunohistochemical method was used to detect the expression of P16 protein in cervical tissue. Results:①Under the biological microscope, the two groups of A and B slices had clear background, positive collocation,accurate location, strong positive intensity, bright and fresh color, and no specific staining, negative and positive staining results were reliable.② In group A, the positive rate of p16 protein in chronic cervicitis group, LSIL group, HSIL group and cervical cancer group were 5.77%, 35.63%, 80.33% and 100.00%, respectively. The positive rate of p16 protein in B group was 5.77%,33.33%, 81.97% and 100.00% respectively in chronic cervicitis group, LSIL group, HSIL group and cervical cancer group.There was no significant difference in the positive rate of P16 protein expression between A and B groups(P >0.05).Conclusions:Using polyhydroxyacrylic acid instead of 10% neutral buffer Faure Marin has no significant difference on the positive rate of P16 protein expression in cervical tissue by immunohistochemical method. Polyhydroxyacrylic acid as a stationary liquid has certain value in the detection of P16 protein expression in cervical tissue by immunohistochemical method.
引文
[1]陈志强,米贤军,陈昂,等.不同体积的4%中性缓冲甲醛固定液对全自动免疫组化法检测乳腺原发性浸润癌Her2蛋白的影响[J].诊断病理学杂志,2017,24(9):709-711.
[2]陈志强,王莹,米贤军,等.不同固定时间和烤片时间对乳腺浸润性导管癌HER-2、p16免疫组化染色的影响[J].临床与实验病理学杂志,2017,33(8):927-928.
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[5]张丽荣,朱筱娟,杨绍娟,等.Fine Fix固定液与福尔马林固定液对组织保存及固定的比较[J].临床与实验病理学杂志,2013,29(5):573-575.
[6]陈志强,王莹,米贤军,等.聚羟基丙烯酸和4%中性缓冲甲醛对苏木精伊红染色及荧光原位杂交检测乳腺癌HER-2基因影响的比较[J].中国癌症杂志,2016,26(2):121-127.
[7]陈志强,王莹,米贤军,等.聚羟基丙烯酸和Van-clear替代传统试剂在FISH法检测宫颈h TERC基因中的应用比较[J].北京大学学报(医学版),2016,48(2):356-360.
[8]陈志强,王莹,叶才果,等.聚羟基丙烯酸和Van-clear在q RTPCR法检测非小细胞肺癌EGFR基因突变中的应用研究[J].西安交通大学学报(医学版),2017,38(5):758-762.
[9]陈志强,王莹,米贤军,等.聚羟基丙烯酸在Wade-Fite苯酚碱性品红法检测结核杆菌中的应用[J].诊断病理学杂志,2017,24(10):783-786.
[10]卢朝辉,陈杰.WHO女性生殖器官肿瘤学分类(第4版)解读[J].中华病理学杂志,2014,43(10):649-650.
[11]中华医学会.临床技术操作规范:病理学分册[M].北京:人民军医出版社,2004:33.
[12]陈志强,王莹,米贤军,等.Van-Clear和二甲苯在荧光原位杂交技术检测宫颈h TERC基因中的应用[J].中南大学学报(医学版),2016,41(4):367-373.
[13]陈志强,王莹,叶才果,等.环保透明脱蜡液Van-Clear与传统试剂在组织处理后HE染色及FISH法检测乳腺癌HER2基因中的比较[J].华中科技大学学报(医学版),2017,46(4):453-458.
[14]陈志强,王莹,米贤军,等.Van-clear替代二甲苯对乳腺癌Her-2蛋白表达的影响[J].诊断病理学杂志,2017,24(12):918-921.
[15]陈志强,米贤军,陈昂,等.不同p H值的40 m L/L磷酸盐缓冲甲醛液固定乳腺癌组织对FISH法检测HER2基因结果的影响[J].西安交通大学学报(医学版),2017,39(3):438-441.
[16]张涛,毛世华,唐良萏.宫颈上皮内瘤变及早期宫颈癌组织中P16、HPV L-1壳蛋白的表达及与HR-HPV载量相关性研究[J].实用妇产科杂志,2016,32(7):536-539.
[17]王荣敏,李雪洁,钱敏,等.CINⅠ的转归及p16INK4a蛋白表达在分流CINⅠ中的临床意义[J].中华妇产科杂志,2015,50(3):210-215.
[18]金蓉蓉,马红伟,陈天羽,等.p16/Ki-67双染检测在意义不明的不典型鳞状细胞分流诊断中的应用[J].中华病理学杂志,2017,46(7):481-484.
[19]郭艳敏,周梅香.p16、Ki-67在宫颈上皮内病变中的表达及意义[J].诊断病理学杂志,2017,24(2):105-107.
[2]陈志强,王莹,米贤军,等.不同固定时间和烤片时间对乳腺浸润性导管癌HER-2、p16免疫组化染色的影响[J].临床与实验病理学杂志,2017,33(8):927-928.
[3]陈志强,王莹,米贤军,等.不同量甲醛固定液对荧光原位杂交法检测乳腺原发性浸润癌HER2基因扩增的影响[J].浙江大学学报(医学版),2017,46(4):439-444.
[4]Zendehdel R,Fazli Z,Mazinani M.Neurotoxicity effect of formaldehyde on occupational exposure and influence of individual susceptibility to some metabolism parameters[J].Environ Monit Assess,2016,188(11):648.
[5]张丽荣,朱筱娟,杨绍娟,等.Fine Fix固定液与福尔马林固定液对组织保存及固定的比较[J].临床与实验病理学杂志,2013,29(5):573-575.
[6]陈志强,王莹,米贤军,等.聚羟基丙烯酸和4%中性缓冲甲醛对苏木精伊红染色及荧光原位杂交检测乳腺癌HER-2基因影响的比较[J].中国癌症杂志,2016,26(2):121-127.
[7]陈志强,王莹,米贤军,等.聚羟基丙烯酸和Van-clear替代传统试剂在FISH法检测宫颈h TERC基因中的应用比较[J].北京大学学报(医学版),2016,48(2):356-360.
[8]陈志强,王莹,叶才果,等.聚羟基丙烯酸和Van-clear在q RTPCR法检测非小细胞肺癌EGFR基因突变中的应用研究[J].西安交通大学学报(医学版),2017,38(5):758-762.
[9]陈志强,王莹,米贤军,等.聚羟基丙烯酸在Wade-Fite苯酚碱性品红法检测结核杆菌中的应用[J].诊断病理学杂志,2017,24(10):783-786.
[10]卢朝辉,陈杰.WHO女性生殖器官肿瘤学分类(第4版)解读[J].中华病理学杂志,2014,43(10):649-650.
[11]中华医学会.临床技术操作规范:病理学分册[M].北京:人民军医出版社,2004:33.
[12]陈志强,王莹,米贤军,等.Van-Clear和二甲苯在荧光原位杂交技术检测宫颈h TERC基因中的应用[J].中南大学学报(医学版),2016,41(4):367-373.
[13]陈志强,王莹,叶才果,等.环保透明脱蜡液Van-Clear与传统试剂在组织处理后HE染色及FISH法检测乳腺癌HER2基因中的比较[J].华中科技大学学报(医学版),2017,46(4):453-458.
[14]陈志强,王莹,米贤军,等.Van-clear替代二甲苯对乳腺癌Her-2蛋白表达的影响[J].诊断病理学杂志,2017,24(12):918-921.
[15]陈志强,米贤军,陈昂,等.不同p H值的40 m L/L磷酸盐缓冲甲醛液固定乳腺癌组织对FISH法检测HER2基因结果的影响[J].西安交通大学学报(医学版),2017,39(3):438-441.
[16]张涛,毛世华,唐良萏.宫颈上皮内瘤变及早期宫颈癌组织中P16、HPV L-1壳蛋白的表达及与HR-HPV载量相关性研究[J].实用妇产科杂志,2016,32(7):536-539.
[17]王荣敏,李雪洁,钱敏,等.CINⅠ的转归及p16INK4a蛋白表达在分流CINⅠ中的临床意义[J].中华妇产科杂志,2015,50(3):210-215.
[18]金蓉蓉,马红伟,陈天羽,等.p16/Ki-67双染检测在意义不明的不典型鳞状细胞分流诊断中的应用[J].中华病理学杂志,2017,46(7):481-484.
[19]郭艳敏,周梅香.p16、Ki-67在宫颈上皮内病变中的表达及意义[J].诊断病理学杂志,2017,24(2):105-107.