阳明腑实证大鼠内毒素血症与肺损伤的相关性及中药的干预作用
|
摘要
目的:探讨阳明腑实证大鼠内毒素血症与肺损伤的相关性并观察大黄游离蒽醌-黄芩总黄酮配伍的治疗作用。方法:90只雄性SD大鼠随机分为生理盐水组、阳明腑实证模型组和阳明腑实证模型+中药组(中药组)。各组大鼠分别在6、12、24h后放血处死并收集肺组织,检测血浆及肺组织匀浆中ET、NO、TNF-α、IL-1及MPO的含量;观察肺病理组织学改变。结果:与模型组比较,中药组动脉血及肺组织匀浆中ET、NO、TNF-α、IL-1含量明显降低(P<0.01,P<0.05);中药组肺损伤程度较模型组明显减轻。结论:阳明腑实证大鼠的肺损伤与内毒素血症密切相关,大黄游离蒽醌-黄芩总黄酮可能通过减少内毒素和炎性因子的释放减轻肺损伤。
Objective: To explore the correlation between endotoxemia and lung injury in rats with Yangming fuviscera excess syndrome, and observe therapeutic effect of synergy with free anthraquinones of rhubarb-toral flavonoids of Scutellariabaicalensis georgi. Methods: A total of 90 Sprage-Dawley(SD) rats were randomly divided into physiological saline group(S group), model of Yangming fu-viscera excess syndrome group(M group) and model plus compatibility of effective components(M+CEC group). Rats in each group were sacrificed after 6 h, 12 h and 24 h by bloodletting. Then the lung tissues and plasma were collected to detect the contents of ET, NO, TNF-α, IL-1 and MPO, and observe the histopathological changes of lung tissues. Results: Compared with M group, the contents of ET, NO, TNF-α and IL-1 in plasma and lung tissue were significantly decreased(P<0.01, P<0.05), and the degree of lung injury in M+CEC group was obviously alleviated. Conclusion: The lung injury was closely related to endotoxemia in rats with Yangming fu-viscera excess syndrome, and the free anthraquinone of rhubarb-total flavonoids of scutellariabaicalensis georgi might reduce lung injury by reducing the release of endotoxin and inflammatory factors.
Objective: To explore the correlation between endotoxemia and lung injury in rats with Yangming fuviscera excess syndrome, and observe therapeutic effect of synergy with free anthraquinones of rhubarb-toral flavonoids of Scutellariabaicalensis georgi. Methods: A total of 90 Sprage-Dawley(SD) rats were randomly divided into physiological saline group(S group), model of Yangming fu-viscera excess syndrome group(M group) and model plus compatibility of effective components(M+CEC group). Rats in each group were sacrificed after 6 h, 12 h and 24 h by bloodletting. Then the lung tissues and plasma were collected to detect the contents of ET, NO, TNF-α, IL-1 and MPO, and observe the histopathological changes of lung tissues. Results: Compared with M group, the contents of ET, NO, TNF-α and IL-1 in plasma and lung tissue were significantly decreased(P<0.01, P<0.05), and the degree of lung injury in M+CEC group was obviously alleviated. Conclusion: The lung injury was closely related to endotoxemia in rats with Yangming fu-viscera excess syndrome, and the free anthraquinone of rhubarb-total flavonoids of scutellariabaicalensis georgi might reduce lung injury by reducing the release of endotoxin and inflammatory factors.
引文
[1]王晓谦,涂君平,蒋会会,等.阳明腑实证动勿肠道黏膜免疫机能变化的研究进展.中国兽医杂志,2016,52(1):77-80
[2]张西波,崔乃强,袁红霞,等.浅谈阳明腑实证与肠源性内毒素血症的相关性.山东中医杂志,2007,26(4):219-221
[3]郑秀丽,杨宇,王宝家,等.从病理状态下肺功能与胃肠功能的变化探讨.“肺与大肠相表里”.中华中医药杂志,2014,29(1):120-123
[4]张经文,陈海龙,张桂信,等.阳明腑实证时肠道屏障损伤与肺损伤相关性的研究进展.中国中西医结合消化杂志,2014,22(11):699-702
[5]樊新荣,王荣田,朱文锋,等.阳明病肠热腑实证大鼠模型的建立与机制.世界华人消化杂志,2007,15(21):2290-2294
[6]山丽梅,赵艳玲,孔维军,等.大鼠胃热证动物模型的建立.中国实验方剂学杂志,2009(2):30-32
[7]荀翀.阳明腑实证大鼠模型的制备及其病理机制研究.大连:大连医科大学,2009
[8]Zhang L,Zhao S,Yuan L,et al.Knockdown of placental growth factor(PLGF)mitigates hyperoxia-induced acute lung injury in neonatal rats:Suppressive effects on NFκB signaling pathway.International Immunopharmacology,2016,38:167
[9]冯立民,陈海龙,关凤林,等.阳明腑实证时内毒素与炎症介质的变化及复方大承气汤的治疗作用.中国中西医结合外科杂志,2003,9(5):351-353
[10]汲海燕.急性肺损伤/急性呼吸窘迫综合征发病机制的研究进展.吉林医学,2014(29):6578-6579
[11]Yoshida T,Betsuyaku T,Nagai K,et al.Discrepancy between myeloperoxidase(mpo)activity and the number of neutrophils in bronchoalveolar lavage fluid(balf)during resolution of lipopolysaccaride(lps)-induced lung inflammation.Denver Colorado,2011:5964-5964
[12]杨娜.泻心汤有效组分抗内毒素的药效及机制研究.成都:成都中医药大学,2007
[13]曲亚楠.“通里攻下法”治疗MODS的临床研究.大连:大连医科大学,2013
[14]邬博,刘彦晶,连丽.大黄的药理作用研究进展.中国中医药现代远程教育,2015,13(20):152-154
[15]王雅芳,李婷,唐正海,等.中药黄芩的化学成分及药理研究进展.中华中医药学刊,2015(1):206-211
[2]张西波,崔乃强,袁红霞,等.浅谈阳明腑实证与肠源性内毒素血症的相关性.山东中医杂志,2007,26(4):219-221
[3]郑秀丽,杨宇,王宝家,等.从病理状态下肺功能与胃肠功能的变化探讨.“肺与大肠相表里”.中华中医药杂志,2014,29(1):120-123
[4]张经文,陈海龙,张桂信,等.阳明腑实证时肠道屏障损伤与肺损伤相关性的研究进展.中国中西医结合消化杂志,2014,22(11):699-702
[5]樊新荣,王荣田,朱文锋,等.阳明病肠热腑实证大鼠模型的建立与机制.世界华人消化杂志,2007,15(21):2290-2294
[6]山丽梅,赵艳玲,孔维军,等.大鼠胃热证动物模型的建立.中国实验方剂学杂志,2009(2):30-32
[7]荀翀.阳明腑实证大鼠模型的制备及其病理机制研究.大连:大连医科大学,2009
[8]Zhang L,Zhao S,Yuan L,et al.Knockdown of placental growth factor(PLGF)mitigates hyperoxia-induced acute lung injury in neonatal rats:Suppressive effects on NFκB signaling pathway.International Immunopharmacology,2016,38:167
[9]冯立民,陈海龙,关凤林,等.阳明腑实证时内毒素与炎症介质的变化及复方大承气汤的治疗作用.中国中西医结合外科杂志,2003,9(5):351-353
[10]汲海燕.急性肺损伤/急性呼吸窘迫综合征发病机制的研究进展.吉林医学,2014(29):6578-6579
[11]Yoshida T,Betsuyaku T,Nagai K,et al.Discrepancy between myeloperoxidase(mpo)activity and the number of neutrophils in bronchoalveolar lavage fluid(balf)during resolution of lipopolysaccaride(lps)-induced lung inflammation.Denver Colorado,2011:5964-5964
[12]杨娜.泻心汤有效组分抗内毒素的药效及机制研究.成都:成都中医药大学,2007
[13]曲亚楠.“通里攻下法”治疗MODS的临床研究.大连:大连医科大学,2013
[14]邬博,刘彦晶,连丽.大黄的药理作用研究进展.中国中医药现代远程教育,2015,13(20):152-154
[15]王雅芳,李婷,唐正海,等.中药黄芩的化学成分及药理研究进展.中华中医药学刊,2015(1):206-211