HPLC法测定温性经筋通贴膏中柚皮苷的含量
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摘要
目的测定温性经筋通贴膏中柚皮苷的含量。方法采用高效液相色谱法(HPLC),色谱柱为Hypersil C_(18)分析柱(250mm×4.6mm,5μm);流动相为甲醇-醋酸-水(30∶4∶70),流速1.0mL·min~(-1),检测波长283nm,柱温为30℃。结果柚皮苷在0.0146～0.4671mg·mL~(-1)范围具良好线性;平均回收率为99.25%(RSD=0.84%)。结论本法快速、灵敏、准确,稳定性、重现性良好,可用于温性经筋通贴膏的质量控制。
OBJECTIVE To determination the Naringin in WenXing JingJingTong Plaster.METHODS The HPLC separation was performed on Hypersil-C_(18)(4.6 mm×250 mm,5μm) column with a mixture methanol-acetic acid-water(30∶4∶70) as the mobile phase,at a flow rate of 1 mL·min~(-1).Detection wavelength was 283 nm and column temperature was 30℃.RESULTS The linearity of naringin was in the range of 0.0146-0.4671 mg·mL~(-1) and the average recovery was 99.25%,RSD=0.84%(n=6).CONCLUSION The method is sensitive,rapid and accurate,stable,reproducible and can be used to control the quality of WenXing JingJingTong Plaster.
OBJECTIVE To determination the Naringin in WenXing JingJingTong Plaster.METHODS The HPLC separation was performed on Hypersil-C_(18)(4.6 mm×250 mm,5μm) column with a mixture methanol-acetic acid-water(30∶4∶70) as the mobile phase,at a flow rate of 1 mL·min~(-1).Detection wavelength was 283 nm and column temperature was 30℃.RESULTS The linearity of naringin was in the range of 0.0146-0.4671 mg·mL~(-1) and the average recovery was 99.25%,RSD=0.84%(n=6).CONCLUSION The method is sensitive,rapid and accurate,stable,reproducible and can be used to control the quality of WenXing JingJingTong Plaster.
引文
[1]吴康郁,朱群娣,黄伟斌,等.温性经筋通贴膏治疗关节炎的临床观察[J].中药材,2015,38(06):1331-1333.
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[2]刘东文,陈淑映,李子鸿.巴布剂渭良伤科贴膏的质量标准研究[J].中国药房,2014,25(27):2544-2546.
[3]林葳,胡健敏,陈恩瑜.HPLC测定接骨丹片中柚皮苷的含量[J].海峡药学,2009,21(12):96-97.
[4]林雨昕,黄丽梅.反相高效液相色谱法测定止咳类药品中柚皮苷的含量[J].海峡药学,2017,29(01):39-42.
[5]国家药典委员会编.中国药典一部[S].北京:中国医药科技出版社,2015.
[6]文芳杰,周勇,游绍雪.高效液相色谱法测定地龙膏中柚皮苷含量[J].亚太传统医药,2015,11(21):41-43.
[7]伍奕,蒋晓煌,蒋孟良,等.骨碎补中柚皮苷含量测定方法的优化研究[J].湖南中医药大学学报,2010,30(3):48-50.
[8]秦文兵,岐琳.HPLC法测定骨伤胶囊中柚皮苷的含量[J].西北药学杂志,2009,24(3):168-169.