黑果小檗提取物对高脂血症大鼠血脂、血液流变学和脂质过氧化水平的影响
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摘要
目的研究黑果小檗提取物对高脂血症大鼠血脂、血液流变学和脂质过氧化水平的影响。方法将50只SD大鼠随机分成模型组,辛伐他汀组(5mg/kg),黑果小檗提取物低、中、高剂量组(100、200,400mg/kg),每天灌胃给予高脂乳剂(10mL/kg),连续灌胃20d;另取10只SD大鼠作为正常对照组,灌胃给予蒸馏水(10mL/kg),连续灌胃20d。各组大鼠给药6w后腹主动脉取血,全血采用全自动血流变仪测定全血粘度、血浆粘度、红细胞压积、聚集指数、变形指数、刚性指数,分离血清采用全自动生化仪测定胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C),并采用全自动酶标仪测定丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)。结果与模型组比较,黑果小檗提取物低、中、高剂量组大鼠血清TC、TG、LDL-C、ALT、AST、全血粘度、血浆粘度、红细胞压积、MDA降低,HDL-C、红细胞变形能力、SOD、GSH-Px升高,差异有统计学意义(P<0.05),其中黑果小檗提取物高剂量组作用最为明显:TC(2.87±0.36)mmol/L、TG(0.67±0.06)mmol/L、LDL-C(0.93±0.19)mmol/L、ALT(42.28±6.36)U/L、AST(89.25±9.31)U/L、全血粘度-低切(8.22±1.96)mPa/s、全血粘度-中切(3.88±0.86 mPa/s)、全血粘度-高切(3.45±0.65)mPa/s、血浆粘度(1.19±0.12)mPa/s、红细胞压积(52.49±7.56)%、MDA(5.19±0.46)mmol/L、HDL-C(0.95±0.13)mmol/L、SOD(183.64±22.51)U/L、GSH-Px(42.80±6.36)pmol/L。结论黑果小檗提取物对高脂血症大鼠具有降血脂作用,可改善血液流变学并减轻脂代谢紊乱的过氧化损伤。
Objective To study the activity of Beberis heteropodaextracts on blood lipid,hemorheology and antioxidation of hyperlipidemia rats.Methods 50 SD rats were divided into 5 groups as model group,simvastatin group(5 mg/kg),low(100 mg/kg),medium(200 mg/kg)and high(400 mg/kg)dose of Beberis heteropodagroups,given high fat emulsion(10 mL/kg)by intragastric administration for 20 d,10 SD rats were given distilled water for normal control group.After 6 weeks of treatment,whole blood viscosity,plasma viscosity,hematokrit,indexes of erythrocyte aggregation,deformation and rigidity by automatic blood rheometer.The levels of TC,TG,HDL-C and LDL-C in serum were tested by automatic biochemical analyzer.The levels of ALT,AST,SOD,GSH-Px and MDA were tested by microplate absorbance reader.Results Compared with model group,the levels of TC,TG,LDL-C,ALT,AST,MDA,blood viscosity,hematokrit,erythrocyte aggregation and rigidity index were decreased,the levels of HDL-C,SOD,GSH-Px and erythrocyte deformation index were increased(P <0.05).High(400 mg/kg)dose of Beberis heteropoda group showed the most significant effect:TC(2.87±0.36)mmol/L,TG(0.67±0.06)mmol/L,LDL-C(0.93±0.19)mmol/L,ALT(42.28±6.36)U/L,AST(89.25±9.31)U/L,blood viscosity-low shear(8.22±1.96)mPa/s,blood viscosity-middle shear(3.88±0.86)mPa/s,blood viscosityhigh shear(3.45±0.65)mPa/s,plasma viscosity(1.19±0.12)mPa/s,hematokrit(52.49±7.56)%,MDA(5.19±0.46)mmol/L,HDL-C(0.95±0.13)mmol/L,SOD(183.64±22.51)U/L,GSH-Px(42.80±6.36)pmol/L.Conclusion Beberis heteropoda extracts have activities of regulating blood-lipid,improving hemorheology and inhibiting peroxidation damage in hyperlipidemia rats.
Objective To study the activity of Beberis heteropodaextracts on blood lipid,hemorheology and antioxidation of hyperlipidemia rats.Methods 50 SD rats were divided into 5 groups as model group,simvastatin group(5 mg/kg),low(100 mg/kg),medium(200 mg/kg)and high(400 mg/kg)dose of Beberis heteropodagroups,given high fat emulsion(10 mL/kg)by intragastric administration for 20 d,10 SD rats were given distilled water for normal control group.After 6 weeks of treatment,whole blood viscosity,plasma viscosity,hematokrit,indexes of erythrocyte aggregation,deformation and rigidity by automatic blood rheometer.The levels of TC,TG,HDL-C and LDL-C in serum were tested by automatic biochemical analyzer.The levels of ALT,AST,SOD,GSH-Px and MDA were tested by microplate absorbance reader.Results Compared with model group,the levels of TC,TG,LDL-C,ALT,AST,MDA,blood viscosity,hematokrit,erythrocyte aggregation and rigidity index were decreased,the levels of HDL-C,SOD,GSH-Px and erythrocyte deformation index were increased(P <0.05).High(400 mg/kg)dose of Beberis heteropoda group showed the most significant effect:TC(2.87±0.36)mmol/L,TG(0.67±0.06)mmol/L,LDL-C(0.93±0.19)mmol/L,ALT(42.28±6.36)U/L,AST(89.25±9.31)U/L,blood viscosity-low shear(8.22±1.96)mPa/s,blood viscosity-middle shear(3.88±0.86)mPa/s,blood viscosityhigh shear(3.45±0.65)mPa/s,plasma viscosity(1.19±0.12)mPa/s,hematokrit(52.49±7.56)%,MDA(5.19±0.46)mmol/L,HDL-C(0.95±0.13)mmol/L,SOD(183.64±22.51)U/L,GSH-Px(42.80±6.36)pmol/L.Conclusion Beberis heteropoda extracts have activities of regulating blood-lipid,improving hemorheology and inhibiting peroxidation damage in hyperlipidemia rats.
引文
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[9]陈玉兴,杜铁良,赵自明,等.复方姜黄微囊对高脂血症大鼠血清脂质及肝脏病理的影响[J].中成药,2012,34(6):1025-1029.
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[11]WREE A,KAHRAMAN A,GERKEN G,et al.Obesity affects the liver-the link between adipocytes and hepatocytes[J].Digestion,2011,83(1/2):124-133.
[2]王仁.哈萨克药志[M].乌鲁木齐:新疆科学技术出版社,2009:96.
[3]SUN L L,GAO W,ZHANG M M,et al.Composition and antioxidant activity of the anthocyanins of the fruit of Berberis heteropoda Schrenk[J].Molecules.2014,19(11):19078-19096.
[4]李莉,孙颖平,李思栋,等.黑果小檗总黄酮含量测定及体外抗氧化活性研究[J].安徽医药,2013,17(6):934-936.
[5]孙颖平,李莉.新疆特色药材黑果小檗薄层鉴别方法研究[J].西北药学杂志,2013,28(3):231-234.
[6]谭梦辉,于波,谷颖敏,等.香青兰不同部位提取物对大鼠实验性高脂血症的影响[J].中国实验方剂学杂志,2011,17(21):209.
[7]IVERSEN A,JENSEN J S,SCHARLING H,et al.Hypercholesterolaemia and risk of coronary heart disease in the elderly:impact of age:city heart study[J].Eur J Intern Med,2009,20(2):139-142.
[8]熊清平,石莹莹,张强华,等.白藜芦醇对高脂血症大鼠血脂及血液流变学的影响[J].中药药理与临床,2011,22(5):517-521.
[9]陈玉兴,杜铁良,赵自明,等.复方姜黄微囊对高脂血症大鼠血清脂质及肝脏病理的影响[J].中成药,2012,34(6):1025-1029.
[10]GIOVANNI M,ROBERT G,MAURIZIO C.Recent insights into hepatic lipid metabolism in non-alcoholic fatty liver disease(NAFLD)[J].Prog Lipid Res,2009,48(1):19-26.
[11]WREE A,KAHRAMAN A,GERKEN G,et al.Obesity affects the liver-the link between adipocytes and hepatocytes[J].Digestion,2011,83(1/2):124-133.