丁香假单胞菌对亚洲玉米螟幼虫抗寒能力的影响
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摘要
本文用丁香假单胞菌Pseudomonas syringae处理亚洲玉米螟Ostrinia furnacalis(Guenée)幼虫,使用过冷却仪测定过冷却点,分析该菌株对参试幼虫耐寒能力的影响。结果表明,亚洲玉米螟5龄非滞育幼虫抗寒能力比其他龄期非滞育幼虫强。亚洲玉米螟滞育5龄幼虫抗寒能力比非滞育5龄幼虫强。使用丁香假单胞菌处理亚洲玉米螟5龄非滞育和滞育幼虫,24 h后非滞育幼虫的过冷却点和结冰点分别提高了5.14和2.83℃。5龄滞育幼虫过冷却点和结冰点分别提高了11.25和4.65℃。丁香假单胞菌菌悬液(浓度为8×108 cfu/m L)对亚洲玉米螟具有较好的杀虫效果,非滞育5龄幼虫在(15±1)℃条件下处理24 h后平均死亡率39.1%。
Effect of Pseudomonas syringae on cold tolerance of the Asian corn borer larvae, Ostrinia furnacalis(Guenée), was analyzed by measureing the supercooling point. Results indicated that cold resistance of the non diapause 5th instar O. furnacalis larvae was stronger than that of other age groups. The cold resistance of the 5th instar diapause O. furnacalis larvae was higher than that of non diapause 5th instar larvae. When treated with Pseudomonas syringae for 24 h, the supercooling point and freezing point of non diapause 5th instar O. furnacalis larvae increased by 5.14 ℃ and 2.83 ℃, respectively; and diapause 5th instar larvae increased by 11.46 ℃ and 4.65 ℃, respectively. P. syringae(at concentration of 8×108 cfu/ml) had a good insecticidal effect on O. furnacalis, with mortality rate of non-diapause 5th instar larvae being 39.1% under 15 ℃ after 24 h.
Effect of Pseudomonas syringae on cold tolerance of the Asian corn borer larvae, Ostrinia furnacalis(Guenée), was analyzed by measureing the supercooling point. Results indicated that cold resistance of the non diapause 5th instar O. furnacalis larvae was stronger than that of other age groups. The cold resistance of the 5th instar diapause O. furnacalis larvae was higher than that of non diapause 5th instar larvae. When treated with Pseudomonas syringae for 24 h, the supercooling point and freezing point of non diapause 5th instar O. furnacalis larvae increased by 5.14 ℃ and 2.83 ℃, respectively; and diapause 5th instar larvae increased by 11.46 ℃ and 4.65 ℃, respectively. P. syringae(at concentration of 8×108 cfu/ml) had a good insecticidal effect on O. furnacalis, with mortality rate of non-diapause 5th instar larvae being 39.1% under 15 ℃ after 24 h.
引文
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[21]杨凤连,程家森,罗佑珍,等.冰核活性细菌(INAB)对菜青虫血淋巴免疫系统影响的研究[J].昆虫知识,2005,42(4):391-394.
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[23]唐朝荣,孙福在,赵廷昌,等.利用细菌冰核基因构建促冻杀虫基因工程菌[J].科学通报,2003,48(1):66-71.
[24]唐朝荣.冰核细菌Erwinia ananas 110冰核基因克隆、序列分析和促冻杀虫基因工程菌构建[J].植物病理学报,2002,32(4):373-374.
[25]乔利,郑坚武,成卫宁,等.不同饲料配方对亚洲玉米螟生长发育和繁殖的影响[J].西北农林科技大学学报(自然科学版),2008,36(5):109-112.
[26]Got B.Determination of instar of the european corn borer(Lepidoptera:Pyralidae)based on a distribution model of head capsule widths[J].Annals of the Entomological Society of America,1988,81(1):91-98.
[27]张洪刚,鲁新,何康来,等.亚洲玉米螟抗寒及低温生存对策[J].植物保护学报,2010,37(5):398-402.
[2]李浩,晓榕,保平,等.沙葱萤叶甲的过冷却能力与抗寒性[J].昆虫学报,2014,7(2):212-217.
[3]李娜,周晓榕,庞保平,等.宽翅曲背蝗卵的过冷却能力与抗寒性[J].应用生态学报,2014,25(7):2099-2104.
[4]黄治轶,昊昱,杜源良,等.亚洲柑橘木虱和柚喀木虱过冷却点和体液结冰点测定[J].环境昆虫学报,2015,37(1):1-7.
[5]李帅强,张统书,刘宁,等.辽宁省二代亚洲玉米螟为害防治的经济阈值[J].生态学杂志,2017,6(1):104-109.
[6]胡志凤,文鹏,丛斌,等.亚洲玉米螟生物防治研究进展[J].黑龙江农业科学,2013,(10):145-149.
[7]王丹丹,王清明,等.丁香假单胞菌的分子生物学研究进展[J].西北农业学报,2017,26(4):487-496.
[8]刘欢欢,焦伟,乔保明,等.一种利用铁离子螯合剂恢复丁香假单胞菌菌落荧光的方法[J].中国农业科技导报,2012,14(4):142-147.
[9]孟姗,何彦,张中娜,等.丁香假单胞菌冰核基因ina Q变速箱启动子结构与活性分析[J].氨基酸和生物资源,2014,36(4):47-53.
[10]李明亚,林陈水.冰晶核蛋白及其在细菌表面展示技术中的应用[J].氨基酸和生物资源,2016,38(2):7-11.
[11]张耀东,曹阳,于林平,等.冰核活性细菌对储粮害虫耐冻性的影响[J].郑州粮食学院学报,1998(2):1-5.
[12]姜莉,缪卫国,努尔孜亚,等.冰核细菌的研究、应用现状和前景[J].新疆农业科学,2002,39(5):271-274.
[13]谢翔,王玉,刘汉林,等.冰核细菌及冰核基因的应用研究进展[J].生物技术通报,2006(S):109-113.
[14]段波,李燕,罗佑珍,等.运用冰核活性细菌降低害虫的抗寒能力[J].云南农业大学学报,2004,19(3):260-264.
[15]Strong Gunderson J M,Lee M R,Lee R E.Ingestion of ice-nucleating active bacteria increases the supercooling point of the lady beetle Hippodamia convergens[J].Journal of Insect Physiology,1990,36(3):153-157.
[16]Lee R E J,Strong-Gunderson J M,Lee M R,et al.Ice-nucleating active bacteria decrease the cold-hardiness of stored grain insects[J].Journal of Economic Entomology,1992,85(2):371-374.
[17]Strong-Gunderson J M,Lee R E,Lee M R.Topical application of ice-nucleating active bacteria decreases insect cold tolerancet[J].Applied And Environmental Microbioloblogy,1992,58(9):2711-2716.
[18]Castrillo L A,Lee R E,Wyman J A,et al.Field Persistence of ice nucleating bacteria in overwintering colorado potato beetles[J].Journal of Biolobical Control,2001,21(1):11-18.
[19]冯玉香,何维勋.细菌冰核提高印度谷螟过冷却点的研究[J].昆虫学报,1996,39(1):53-57.
[20]孙福在,邢炜,张永祥,等.利用冰核细菌对光肩星天牛幼虫促冻杀虫的初步研究[J].林业科学研究,1997,10(1):96-99.
[21]杨凤连,程家森,罗佑珍,等.冰核活性细菌(INAB)对菜青虫血淋巴免疫系统影响的研究[J].昆虫知识,2005,42(4):391-394.
[22]冯玉香,何维勋.冰核真菌削弱赤拟谷盗抗寒力的初步研究[J].昆虫学报.2002,45(1):148-151.
[23]唐朝荣,孙福在,赵廷昌,等.利用细菌冰核基因构建促冻杀虫基因工程菌[J].科学通报,2003,48(1):66-71.
[24]唐朝荣.冰核细菌Erwinia ananas 110冰核基因克隆、序列分析和促冻杀虫基因工程菌构建[J].植物病理学报,2002,32(4):373-374.
[25]乔利,郑坚武,成卫宁,等.不同饲料配方对亚洲玉米螟生长发育和繁殖的影响[J].西北农林科技大学学报(自然科学版),2008,36(5):109-112.
[26]Got B.Determination of instar of the european corn borer(Lepidoptera:Pyralidae)based on a distribution model of head capsule widths[J].Annals of the Entomological Society of America,1988,81(1):91-98.
[27]张洪刚,鲁新,何康来,等.亚洲玉米螟抗寒及低温生存对策[J].植物保护学报,2010,37(5):398-402.