降钙素基因相关肽、血管活性肠肽在勃起功能障碍大鼠模型阴茎组织中表达及作用机制的研究
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摘要
目的:检测ED大鼠模型阴茎组织中降钙素基因相关肽(CGRP)、血管活性肠肽(VIP)的表达,并探讨两者在ED中的作用机制。方法:100只正常雄性SD大鼠,随机抽取10只为正常对照组,余90只为造模组,采用湿寒饮食和环境的干预条件建立ED大鼠模型,20周后通过阿朴吗啡(APO)勃起实验,筛选出44只ED大鼠模型,并随机将其分为ED模型组、自然恢复组、给药组,伊木萨克片250 mg/kg干预2～3周后,免疫组化及Western印迹方法检测阴茎组织中CGRP、VIP的表达。结果:免疫组化检测大鼠阴茎组织中CGRP表达:ED模型组(150. 0±43. 3)、自然恢复组(165. 9±40. 7)较正常对照组(227. 3±42. 5)组明显减少(P <0. 05);给药组(255. 0±38. 7)组较ED模型组(150. 0±43. 3)、自然恢复组(165. 9±40. 7)显著升高(P <0. 05)。免疫组化检测大鼠阴茎组织中VIP表达:ED模型组(36. 4±13. 1)、自然恢复组(67. 5±29. 0)较正常对照组(175. 0±45. 6)显著降低(P <0. 05);给药组(167. 5±42. 6)较ED模型组(36. 4±13. 1)、自然恢复组(67. 5±29. 0)显著升高(P <0. 05)。结论:ED大鼠模型阴茎组织中CGRP、VIP明显减少,伊木萨克片可能通过上调CGRP、VIP表达对ED大鼠发挥作用。
Objective: To determine the expressions of calcitonin gene-related peptide( CGRP) and vasoactive intestinal peptide( VIP) in the penile tissue of the ED rat model and explore their action mechanisms. Methods: An ED model was established in 44 mature male SD rats by feeding them on a spinach + coriander diet in a cold-wet environment and another 10 were taken as normal controls. Then the model rats were randomly divided into an ED model control group( n = 15) treated by gavage of distilled water in the same modeling environment,a spontaneous recovery group( n = 15) treated by gavage of distilled water in the normal environment,and a medication group( n = 14) treated intragastrically with Yimusake Tablets at 250 mg/kg qd. After 2-3 weeks of intervention,the expressions of CGRP and VIP in the penile tissue were detected by immunohistochemistry and Western blot. Results: Immunohistochemistry showed that,after 2 weeks of intervention,both the expressions of CGRP and VIP in the rat penile tissue were significantly lower in the ED model control( 150. 0 ± 43. 3 and 36. 4 ± 13. 1) and the spontaneous recovery group( 165. 9 ± 40. 7 and 67. 5 ± 29. 0) than in the normal control( 227. 3 ± 42. 5 and 175. 0 ± 45. 6)( P < 0. 05),but remarkably higher in the medication group( 255. 0 ± 38. 7 and 167. 5 ± 42. 6) than those in the ED model control and spontaneous recovery groups( P < 0. 05).Conclusion: The expressions of CGRP and VIP were significantly down-regulated in the ED rat model,and Yimusake Tablets improved ED by up-regulating their expressions.
Objective: To determine the expressions of calcitonin gene-related peptide( CGRP) and vasoactive intestinal peptide( VIP) in the penile tissue of the ED rat model and explore their action mechanisms. Methods: An ED model was established in 44 mature male SD rats by feeding them on a spinach + coriander diet in a cold-wet environment and another 10 were taken as normal controls. Then the model rats were randomly divided into an ED model control group( n = 15) treated by gavage of distilled water in the same modeling environment,a spontaneous recovery group( n = 15) treated by gavage of distilled water in the normal environment,and a medication group( n = 14) treated intragastrically with Yimusake Tablets at 250 mg/kg qd. After 2-3 weeks of intervention,the expressions of CGRP and VIP in the penile tissue were detected by immunohistochemistry and Western blot. Results: Immunohistochemistry showed that,after 2 weeks of intervention,both the expressions of CGRP and VIP in the rat penile tissue were significantly lower in the ED model control( 150. 0 ± 43. 3 and 36. 4 ± 13. 1) and the spontaneous recovery group( 165. 9 ± 40. 7 and 67. 5 ± 29. 0) than in the normal control( 227. 3 ± 42. 5 and 175. 0 ± 45. 6)( P < 0. 05),but remarkably higher in the medication group( 255. 0 ± 38. 7 and 167. 5 ± 42. 6) than those in the ED model control and spontaneous recovery groups( P < 0. 05).Conclusion: The expressions of CGRP and VIP were significantly down-regulated in the ED rat model,and Yimusake Tablets improved ED by up-regulating their expressions.
引文
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[3]张小宁,陈道中,郑宇辉,等.降钙素基因相关肽转基因对同种异体移植血管病变的防治作用.中华医学杂志,2008,88(45):3217-3221.
[4]李媛媛.胃癌中VIP表达与癌组织中炎性细胞内抗原呈递分子表达的相关性研究(硕士论文).南昌:南昌大学,2012.
[5]朱彦刘莹,买买提祖农·买苏尔,刘凤霞,等.伊木萨克片对异常黏液质型阳痿病证模型大鼠阴茎组织形态学的影响.中国男科学杂志,2015,29(6):9-14.
[6]Vlachopoulos C,Ioakeimidis N,Terentes-Printzios D,et al.The triad:Erectile dysfunction-endothelial dysfunction-cardiovascular disease.Curr Pharm Des,2008,35(14):3700-3714.
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[9]阿莱·巴合提汗,毛吾兰·买买提依明,张盼盼,等.异常黏液质证候大鼠甲状腺轴改变的研究.新疆医科大学学报,2016,39(5):538-541,545.
[10]毛吾兰·买买提依明,张盼盼,景玉宏,等.异常黏液质证候与阳痿病证大鼠模型肾上腺轴改变的研究.新疆医科大学学报,2016,39(5):530-534.
[11]刘凤霞,李华强,斯依提·阿木提,等.一种环境激素联合冷应激大鼠模型阴茎组织中Sprouty 2、ERK1/2表达的研究.现代生物医学进展,2018,18(18):3431-3435.
[12]徐红涛,李玉华,王旻晨,等.CGRP对ET-1诱导的大鼠血管平滑肌细胞表型转化和增殖的影响.江苏医药,2015,41(13):1499-1501,封3.
[13]毛吾兰·买买提依明,张盼盼,刘凤霞,等.维医异常黏液质型阳痿病证大鼠模型阴茎组织中ET-1的改变及其生物学意义.新疆医科大学学报,2016,39(5):535-537.
[14]蒋明.血管活性肠肽与心脏血管活动.重庆医学,2011,40(10):1026-1028.
[15]陈潇毅,陈云飞.血管活性肠肽免疫调节作用的研究进展.细胞与分子免疫学杂志,2012,28(10):1107-1109.
[16]王娟.血管活性肠肽及其受体与支气管哮喘.国际儿科学杂志,2018,(6):422-425.
[2]全海燕.CGRP通过c AMP/PPARγ/e NOS途径改善血管内皮细胞胰岛素抵抗(硕士论文).衡阳:南华大学,2015.
[3]张小宁,陈道中,郑宇辉,等.降钙素基因相关肽转基因对同种异体移植血管病变的防治作用.中华医学杂志,2008,88(45):3217-3221.
[4]李媛媛.胃癌中VIP表达与癌组织中炎性细胞内抗原呈递分子表达的相关性研究(硕士论文).南昌:南昌大学,2012.
[5]朱彦刘莹,买买提祖农·买苏尔,刘凤霞,等.伊木萨克片对异常黏液质型阳痿病证模型大鼠阴茎组织形态学的影响.中国男科学杂志,2015,29(6):9-14.
[6]Vlachopoulos C,Ioakeimidis N,Terentes-Printzios D,et al.The triad:Erectile dysfunction-endothelial dysfunction-cardiovascular disease.Curr Pharm Des,2008,35(14):3700-3714.
[7]Montorsi P,Ravagnani PM,Galli S,et al.Association between erectile dysfunction and coronary artery disease:Matching the right target with the right test in the right patient.Eur Urol,2006,50(4):721-731.
[8]刘凤霞,斯依提·阿木提,阿卜杜热伊木江·如则,等.环境激素联合冷应激建立勃起功能障碍大鼠模型的实验研究.中国男科学杂志,2018,32(5):11-15.
[9]阿莱·巴合提汗,毛吾兰·买买提依明,张盼盼,等.异常黏液质证候大鼠甲状腺轴改变的研究.新疆医科大学学报,2016,39(5):538-541,545.
[10]毛吾兰·买买提依明,张盼盼,景玉宏,等.异常黏液质证候与阳痿病证大鼠模型肾上腺轴改变的研究.新疆医科大学学报,2016,39(5):530-534.
[11]刘凤霞,李华强,斯依提·阿木提,等.一种环境激素联合冷应激大鼠模型阴茎组织中Sprouty 2、ERK1/2表达的研究.现代生物医学进展,2018,18(18):3431-3435.
[12]徐红涛,李玉华,王旻晨,等.CGRP对ET-1诱导的大鼠血管平滑肌细胞表型转化和增殖的影响.江苏医药,2015,41(13):1499-1501,封3.
[13]毛吾兰·买买提依明,张盼盼,刘凤霞,等.维医异常黏液质型阳痿病证大鼠模型阴茎组织中ET-1的改变及其生物学意义.新疆医科大学学报,2016,39(5):535-537.
[14]蒋明.血管活性肠肽与心脏血管活动.重庆医学,2011,40(10):1026-1028.
[15]陈潇毅,陈云飞.血管活性肠肽免疫调节作用的研究进展.细胞与分子免疫学杂志,2012,28(10):1107-1109.
[16]王娟.血管活性肠肽及其受体与支气管哮喘.国际儿科学杂志,2018,(6):422-425.