响应面法优化黑种草皂苷A双水相提取工艺研究
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摘要
目的优化黑种草皂苷A双水相提取工艺。方法采用响应面法,以黑种草皂苷A提取率为指标,考察料液比、硫酸铵用量、乙醇浓度和超声时间对黑种草皂苷A提取的影响。结果黑种草皂苷A最佳提取工艺为:料液比1∶40,硫酸铵浓度0.35g/mL,乙醇浓度47%,超声时间30min,在此条件下黑种草皂苷A的提取率为3.15%。结论该工艺简单、可靠,适用于黑种草皂苷A的提取。
Objective To optimize the aqueous two-phase extraction of Nigegllanoside A by response surface methodology.Method Using response surface design,and the extraction rate of Nigegllanoside A was taken as the index,to examine the influence of the ratio of solvent to material,ammonium sulfate dosage,ethanol concentration and ultrasonic time on extraction rate of Nigegllanoside A.Results The optimum extraction plan was:1∶4for solid-liquid ratio,0.35g/mL for ammonium sulfate dosage,47%for ethanol concentration,ultrasonic time at 30 min,under above condition,the extraction rate of Nigegllanoside A was 3.15%.Conclusion This method is easy to operate and cost-effective for the extraction of Nigegllanoside A.
Objective To optimize the aqueous two-phase extraction of Nigegllanoside A by response surface methodology.Method Using response surface design,and the extraction rate of Nigegllanoside A was taken as the index,to examine the influence of the ratio of solvent to material,ammonium sulfate dosage,ethanol concentration and ultrasonic time on extraction rate of Nigegllanoside A.Results The optimum extraction plan was:1∶4for solid-liquid ratio,0.35g/mL for ammonium sulfate dosage,47%for ethanol concentration,ultrasonic time at 30 min,under above condition,the extraction rate of Nigegllanoside A was 3.15%.Conclusion This method is easy to operate and cost-effective for the extraction of Nigegllanoside A.
引文
[1]刘勇民.维吾尔药志[M].乌鲁木齐:新疆科技卫生出版社,1999:515-518.
[2]赵军,徐芳,刘亚婷,等.瘤果黑种草子化学成分研究[J].中药材,2012,35(12):1956-1958.
[3]信学雷,薛慧清,阿吉艾克拜尔·艾萨,等.黑种草属植物研究进展[J].时珍国医国药,2008,19(6):1514-1517.
[4]中华人民共和国国家药典委员会.中国药典(一部)[S].北京:中国医药科技出版社,2010:324-325.
[5]国家中医药管理局《中华本草》编委会.中华本草(维吾尔药卷)[M].上海:上海科技出版社,2005:364-366.
[6]金蕾明,赵军,徐芳,等.瘤果黑种草子总皂苷提取工艺研究[J].新疆医科大学学报,2011,34(10):1075-1078.
[7]魏永春,张卫,李冲.皂苷的提取与纯化工艺研究进展[J].广东化工,2008,11(35):58-61.
[8]程昆木.桔梗总皂苷的提取及分析[J].现代医药卫生,2012,28(2):288-289.
[9]张婷婷,李静,王成永,等.木瓜总皂苷提取工艺研究[J].安徽医药,2009,13(2):143-144.
[10]陈志红,李一澍,卢国杰,等.知母总皂苷提取分离方法研究[J].应用化工,2008,37(8):841-843.
[11]周倩,施洋,兰卫,等.黑桑色素提取工艺的优化研究[J].新疆医科大学学报,2015,38(3):288-290.
[12]赵凤平,贾成友,张传辉,等.乙醇-硫酸铵双水相提取三七总皂苷工艺的优化[J].中成药,2016,38(9):2059-2062.
[13]张丙云,魏俊青,郭涛,等.超声辅助双水相体系提取花椒果皮总生物碱研究[J].食品工业科技,2015,36(1):201-206.
[14]付艳丽,高云涛,李竟春,等.星点设计-响应面法优化傣药竹叶兰总黄酮超声-双水相提取工艺[J].中成药,2010,32(12):2059-2062.
[15]高云涛,蒋进超,张宝通.星点设计-效应面优化双水相-超声提取珍珠草多酚[J].食品与发酵工业,2011,37(5):189-193.
[2]赵军,徐芳,刘亚婷,等.瘤果黑种草子化学成分研究[J].中药材,2012,35(12):1956-1958.
[3]信学雷,薛慧清,阿吉艾克拜尔·艾萨,等.黑种草属植物研究进展[J].时珍国医国药,2008,19(6):1514-1517.
[4]中华人民共和国国家药典委员会.中国药典(一部)[S].北京:中国医药科技出版社,2010:324-325.
[5]国家中医药管理局《中华本草》编委会.中华本草(维吾尔药卷)[M].上海:上海科技出版社,2005:364-366.
[6]金蕾明,赵军,徐芳,等.瘤果黑种草子总皂苷提取工艺研究[J].新疆医科大学学报,2011,34(10):1075-1078.
[7]魏永春,张卫,李冲.皂苷的提取与纯化工艺研究进展[J].广东化工,2008,11(35):58-61.
[8]程昆木.桔梗总皂苷的提取及分析[J].现代医药卫生,2012,28(2):288-289.
[9]张婷婷,李静,王成永,等.木瓜总皂苷提取工艺研究[J].安徽医药,2009,13(2):143-144.
[10]陈志红,李一澍,卢国杰,等.知母总皂苷提取分离方法研究[J].应用化工,2008,37(8):841-843.
[11]周倩,施洋,兰卫,等.黑桑色素提取工艺的优化研究[J].新疆医科大学学报,2015,38(3):288-290.
[12]赵凤平,贾成友,张传辉,等.乙醇-硫酸铵双水相提取三七总皂苷工艺的优化[J].中成药,2016,38(9):2059-2062.
[13]张丙云,魏俊青,郭涛,等.超声辅助双水相体系提取花椒果皮总生物碱研究[J].食品工业科技,2015,36(1):201-206.
[14]付艳丽,高云涛,李竟春,等.星点设计-响应面法优化傣药竹叶兰总黄酮超声-双水相提取工艺[J].中成药,2010,32(12):2059-2062.
[15]高云涛,蒋进超,张宝通.星点设计-效应面优化双水相-超声提取珍珠草多酚[J].食品与发酵工业,2011,37(5):189-193.