双酶混合水解玉米皮渣制备阿魏酰低聚糖研究
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摘要
以玉米皮渣为原料,采用酶解方法制备阿魏酰低聚糖(Feruloyl oligosaccharides,FOs),通过比较不同酶解条件下阿魏酰低聚糖产量的差异及不同酶解方式下玉米皮渣细胞壁结构的变化,确定酶解阿魏酰低聚糖生成的方法,并优化获得玉米皮渣中阿魏酰低聚糖的最佳酶解条件。结果表明:纤维素酶和木聚糖酶混合酶解可使玉米皮渣细胞壁出现溶洞,可有效提高阿魏酰低聚糖产量;经单因素和响应面实验确定双酶混合酶解的最佳条件为混合酶的添加量为15.7 g/L,混合酶中纤维素酶添加比例为70%,酶解1 h,玉米皮渣底物添加量为100 g/L,在此条件下FOs产量最高,为1008.43μmol/L。本研究为双酶混合酶解制备玉米皮渣FOs提供理论依据。
Production of feruloyl oligosaccharides by enzymatic hydrolysis form maize bran was investigated in this paper. In order to explore the reason that influenced the yield of feruloyl oligosaccharides,maize bran was hydrolyzed by different enzymes and the cell wall of maize bran was also determined.In the end,the optimal enzyme hydrolysis condition was optimized by single factor and response surface experiment.Results showed that the mixture of cellulase and xylanase could improve maize bran cell wall dissolving,and could improve the yield of feruloyl oligosaccharides.The optimal condition for FOs production was mixture enzyme addition of 15.7 g/L,which was consist with 70% cellulase addition,and the maize bran material addition was at 100 g/L,and hydrolysis for 1 h,under this condition,the yield of FOs was 1008.43 μmol/L.This result provides theoretical basis for FOs production by compound enzymatic hydrolysis from maize bran.
Production of feruloyl oligosaccharides by enzymatic hydrolysis form maize bran was investigated in this paper. In order to explore the reason that influenced the yield of feruloyl oligosaccharides,maize bran was hydrolyzed by different enzymes and the cell wall of maize bran was also determined.In the end,the optimal enzyme hydrolysis condition was optimized by single factor and response surface experiment.Results showed that the mixture of cellulase and xylanase could improve maize bran cell wall dissolving,and could improve the yield of feruloyl oligosaccharides.The optimal condition for FOs production was mixture enzyme addition of 15.7 g/L,which was consist with 70% cellulase addition,and the maize bran material addition was at 100 g/L,and hydrolysis for 1 h,under this condition,the yield of FOs was 1008.43 μmol/L.This result provides theoretical basis for FOs production by compound enzymatic hydrolysis from maize bran.
引文
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[3]Wang T,Zhu YD,Sun XH.Effect of microfluidisation on antioxidant properties of corn bran[J].Food Chemistry,2014,152:37-45.
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[11]Devin JR,George EI.Production of feruloylated arabinoxylooligosaccharides from maize(Zea mays)bran by microwaveassisted autohydrolysis[J].Food Chemistry,2010,119:1613-1618.
[12]Yuan XP,Wang J,Yao HY.Production of femloyl oligosaccharides from wheat bran insoluble dietary fibre by xylanases from Bacillus subtilis[J].Food Chemistry,2006,95(3),484-492.
[13]解春艳,游雪娇,侯晓强,等.物理处理玉米皮渣对酶法提取阿魏酰低聚糖的影响[J].食品工业科技,2016,37(8):272-275.
[14]曾凤彩,王文侠,宋春丽.酶解玉米麸皮制备阿魏酰低聚糖的研究[J].粮食与饲料工业,2011(2):31-35.
[15]Xie CY,Wu ZY,Guo HZ,et al.Release of feruloylated oligosaccharides from wheat bran through submerged fermentation by edible mushrooms[J].Journal of Basic Microbiology,2014,54(S1):14-20.
[16]Tadashi I.Structure and functions of feruloylated polysaccharides[J].Plant Science,1997,127:111-127.
[17]张晓民.禾本科植物细胞壁的木质化和阿魏酰化[J].中国野生植物资源,2011,30(6):7-13.
[18]张璟,欧仕益,张宁,等.酶解麦麸制备阿魏酸和低聚糖的研究[J].食品科学,2003,24(11):63-67.
[2]Rose DL,Inglett GE,Liu SX.Utilisation of corn(Zea mays)bran and corn fiber in the production of food components[J].Journal of Food Agriculture,2010,90:915-924.
[3]Wang T,Zhu YD,Sun XH.Effect of microfluidisation on antioxidant properties of corn bran[J].Food Chemistry,2014,152:37-45.
[4]解春艳,吴智艳,杜娟,等.阿魏酰低聚糖研究进展[J].食品科学,2012,33(7):336-340.
[5]Ou JY,Sun Z.Feruloylated oligosaccharides:Structure,metabolism and function[J].Journal of Functional Foods,2014,7:90-100.
[6]Yuan XP,Wang J,Yao HY.Feruloyl oligosaccharides stimulate growth of Bifidobacterium bifidum[J].Anaerobe,2005,11:225-229.
[7]Ou JY,Sun Z.Feruloylated oligosaccharides:Structure,metabolism and function[J].Journal of Functional Foods,2014,(7):90-100.
[8]Zhang HJ,Wang J,Liu YL,et al.Wheat bran feruloyl oligosaccharides modulate the phase II detoxifying/antioxidant enzymes via Nrf2 signaling[J].International Journal of Biological Macromolecules,2015,74:150-154.
[9]Wang J,Sun BG,Cao YP,et al.Protection of wheat bran feruloyl oligosaccharides against free radical-induced oxidative damage in normal human erythrocytes[J].Food and Chemical Toxicology,2009,47(7):1591-1599.
[10]Zhang HJ,Wang J,Liu YL,et al.Wheat bran feruloyl oligosaccharides modulate the phase II detoxifying/antioxidant enzymes via Nrf2 signaling[J].International Journal of Biological Macromolecules,2015,74:150-154.
[11]Devin JR,George EI.Production of feruloylated arabinoxylooligosaccharides from maize(Zea mays)bran by microwaveassisted autohydrolysis[J].Food Chemistry,2010,119:1613-1618.
[12]Yuan XP,Wang J,Yao HY.Production of femloyl oligosaccharides from wheat bran insoluble dietary fibre by xylanases from Bacillus subtilis[J].Food Chemistry,2006,95(3),484-492.
[13]解春艳,游雪娇,侯晓强,等.物理处理玉米皮渣对酶法提取阿魏酰低聚糖的影响[J].食品工业科技,2016,37(8):272-275.
[14]曾凤彩,王文侠,宋春丽.酶解玉米麸皮制备阿魏酰低聚糖的研究[J].粮食与饲料工业,2011(2):31-35.
[15]Xie CY,Wu ZY,Guo HZ,et al.Release of feruloylated oligosaccharides from wheat bran through submerged fermentation by edible mushrooms[J].Journal of Basic Microbiology,2014,54(S1):14-20.
[16]Tadashi I.Structure and functions of feruloylated polysaccharides[J].Plant Science,1997,127:111-127.
[17]张晓民.禾本科植物细胞壁的木质化和阿魏酰化[J].中国野生植物资源,2011,30(6):7-13.
[18]张璟,欧仕益,张宁,等.酶解麦麸制备阿魏酸和低聚糖的研究[J].食品科学,2003,24(11):63-67.