酶法制备玉米芯阿魏酰低聚糖的工艺条件优化
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摘要
以玉米芯为试验原料,通过木聚糖酶水解玉米芯不溶性膳食纤维制备阿魏酰低聚糖(feruloylated oligosaccharides,FOs)。以阿魏酰低聚糖含量为评价指标,在单因素试验的基础上,采用响应面分析法对玉米芯FOs的酶法制备工艺条件进行研究,得到最佳工艺条件为:加酶量89.7 mg/L、酶解时间16 h、底物浓度111 g/L、pH3.65、酶解温度53℃,在此条件下,阿魏酰低聚糖的含量为0.764 8 mmol/L。
The corn cob was selected as raw material,the insoluble dietary fiber was hydrolyzed by xylanase to release feruloylated oligosaccharides(FOs). With FOs concentration as response value,the process for enzymatic production of FOs was optimized by using response surface methodology based on the single factor experiments. The optimum conditions were determined as follow:preparing for 16 h at 53 ℃ with the substrate concentration of 111 g/L,addition amount of enzyme 89.7 mg/L. Under the optimized conditions,the maximum concentration of FOs was 0.764 8 mmol/L.
The corn cob was selected as raw material,the insoluble dietary fiber was hydrolyzed by xylanase to release feruloylated oligosaccharides(FOs). With FOs concentration as response value,the process for enzymatic production of FOs was optimized by using response surface methodology based on the single factor experiments. The optimum conditions were determined as follow:preparing for 16 h at 53 ℃ with the substrate concentration of 111 g/L,addition amount of enzyme 89.7 mg/L. Under the optimized conditions,the maximum concentration of FOs was 0.764 8 mmol/L.
引文
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[4] Katapodis P, Christakopoulos P. Enzymic production of feruloyl xylo-oligosaccharides from corn cobs by a family 10 xylanase from Thermoascus aurantiacus[J]. LWT-Food Science and Technology,2008, 41(7):1239-1243
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[6] Smith M M, Hartley R D. Occurrence and nature of ferulic acid substitution of cell-wall polysaccharides in graminaceous plants[J].Carbohydrate Research, 1983,118:65-80
[7]胡楠楠,卢敏,迟燕平.米糠阿魏酰低聚糖提取工艺优化研究[J].食品科技, 2016, 41(11):184-189
[8] Saulnier L, Marot C, Elgorriaga M, et al. Thermal and enzymatic treatments for the release of free ferulic acid from maize bran[J].Carbohydrate Polymers, 2001, 45(3):269-275
[9]陈小娥,方旭波,余辉,等.壳寡糖的薄层层析分析[J].浙江海洋学院学报(自然科学版), 2008, 27(4):361-365
[10]陈洲.济麦22麦麸制备阿魏酰低聚糖及其生理活性研究[D].泰安:山东农业大学, 2014:35-36
[11]刘昊飞,程建军,王蕾.酶法制备豆渣水溶性膳食纤维[J].食品工业科技, 2008,29(5):202-204,207
[12]吕丽爽.何首乌中二苯乙烯苷的制备及抗氧化机理研究[D].无锡:江南大学, 2006:30-31
[2]雷钊,尹达菲,袁建敏.阿拉伯木聚糖和阿拉伯低聚木糖的益生功能研究进展[J].动物营养学报, 2017, 29(2):365-373
[3] Torre P, Aliakbarian B, Rivas B, et al. Release of ferulic acid from corn cobs by alkaline hydrolysis[J]. Biochemical Engineering Journal, 2008, 40(3):500-506
[4] Katapodis P, Christakopoulos P. Enzymic production of feruloyl xylo-oligosaccharides from corn cobs by a family 10 xylanase from Thermoascus aurantiacus[J]. LWT-Food Science and Technology,2008, 41(7):1239-1243
[5]白宇仁,孙元琳,王晓闻,等.阿魏酰低聚糖制备、结构及生物活性研究进展[J].食品研究与开发, 2017, 38(20):214-219
[6] Smith M M, Hartley R D. Occurrence and nature of ferulic acid substitution of cell-wall polysaccharides in graminaceous plants[J].Carbohydrate Research, 1983,118:65-80
[7]胡楠楠,卢敏,迟燕平.米糠阿魏酰低聚糖提取工艺优化研究[J].食品科技, 2016, 41(11):184-189
[8] Saulnier L, Marot C, Elgorriaga M, et al. Thermal and enzymatic treatments for the release of free ferulic acid from maize bran[J].Carbohydrate Polymers, 2001, 45(3):269-275
[9]陈小娥,方旭波,余辉,等.壳寡糖的薄层层析分析[J].浙江海洋学院学报(自然科学版), 2008, 27(4):361-365
[10]陈洲.济麦22麦麸制备阿魏酰低聚糖及其生理活性研究[D].泰安:山东农业大学, 2014:35-36
[11]刘昊飞,程建军,王蕾.酶法制备豆渣水溶性膳食纤维[J].食品工业科技, 2008,29(5):202-204,207
[12]吕丽爽.何首乌中二苯乙烯苷的制备及抗氧化机理研究[D].无锡:江南大学, 2006:30-31