摘要
以玉米芯为试验原料,通过木聚糖酶水解玉米芯不溶性膳食纤维制备阿魏酰低聚糖(feruloylated oligosaccharides,FOs)。以阿魏酰低聚糖含量为评价指标,在单因素试验的基础上,采用响应面分析法对玉米芯FOs的酶法制备工艺条件进行研究,得到最佳工艺条件为:加酶量89.7 mg/L、酶解时间16 h、底物浓度111 g/L、pH3.65、酶解温度53℃,在此条件下,阿魏酰低聚糖的含量为0.764 8 mmol/L。
The corn cob was selected as raw material,the insoluble dietary fiber was hydrolyzed by xylanase to release feruloylated oligosaccharides(FOs). With FOs concentration as response value,the process for enzymatic production of FOs was optimized by using response surface methodology based on the single factor experiments. The optimum conditions were determined as follow:preparing for 16 h at 53 ℃ with the substrate concentration of 111 g/L,addition amount of enzyme 89.7 mg/L. Under the optimized conditions,the maximum concentration of FOs was 0.764 8 mmol/L.
引文
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