串联体结合血糖仪的便携式传感器检测cfDNA
|
摘要
目的:建立一种基于DNA串联体和血糖仪的便携式传感器用于检测肿瘤标志物cfDNA。方法:利用DNA串联体的信号放大机制及磁珠的分离与富集作用,以血糖仪为检测器,建立一种检测肿瘤标志物cfDNA的便携式传感器。结果:在1～316fM范围内呈良好的线性关系,检测限为1fM。结论:该传感器便携可行、灵敏度高、重复性好,为肿瘤标志物的筛查提供了新的方法。
Objective: To establish a portable sensor based on DNA tandem and blood glucose meter for detection of tumor marker cfDNA. Methods: A portable sensor for detecting cfDNA, a tumor marker, was established by using the signal amplification mechanism of DNA tandem and the separation and enrichment of magnetic beads, and using a blood glucose meter as a detector. Results: There was a good linear relationship in the range of 1 ～ 316 fM, and the detection limit was 1 fM. Conclusion: The sensor is portable, feasible, sensitive and reproducible. It provides a new method for screening tumor markers.
Objective: To establish a portable sensor based on DNA tandem and blood glucose meter for detection of tumor marker cfDNA. Methods: A portable sensor for detecting cfDNA, a tumor marker, was established by using the signal amplification mechanism of DNA tandem and the separation and enrichment of magnetic beads, and using a blood glucose meter as a detector. Results: There was a good linear relationship in the range of 1 ～ 316 fM, and the detection limit was 1 fM. Conclusion: The sensor is portable, feasible, sensitive and reproducible. It provides a new method for screening tumor markers.
引文
[1]Kaiser J.Keeping Tabs on Tumor dna[J].Science,2010,327(5969):1074-1074.
[2]Schwarzenbach H,Hoon D S B,Pantel K.Cell-Free Nucleic Acids as Biomarkers in Cancer Patients[J].Nature Reviews Cancer,2011,11(6):426-437.
[3]Kolesnikova E V,Tamkovich S N,Bryzgunova O E,et al.Circulating DNA in the Blood of Gastric Cancer Patients[J].Annals of the New York Academy of Sciences,2008,1137(1):226-231.
[4]Kristensen L S,Hansen L L.PCR-based Methods for Detecting Single-Locus DNA Methylation Biomarkers in Cancer Diagnostics,Prognostics,and Response to Treatment[J].Clinical chemistry,2009,55(8):1471-1483.
[5]Bustin S A,Benes V,Garson J A,et al.The MIQE Guidelines:Minimum Information for Publication of Quantitative Real-time PCRExperiments[J].Clinical Chemistry,2009,55(4):611-622.
[6]Lane A A,Chabner B A.Histone Deacetylase Inhibitors in Cancer Therapy[J].Journal of Clinical Oncology,2009,27(32):5459-5468.
[7]Chen X,Hong CY,Lin JH,et al.Enzyme-free and Label-free Ultrasensitive Electrochemical Detection of Human Immunodeficiency Virus DNA in Biological Samples Based on Long-range Selfassembled DNA Nanostructures[J].Analytical Chemistry,2012,84:8277-8283.
[8]Chen X,Lin YH,Li J,et al.A Simple and Ultrasensitive Electrochemical DNA Biosensor Based on DNA Concatamers[J].Chemical Communicat ions,2011,47:12116-12118.
[9]Hong CY,Chen X,Liu T,et al.Ultrasensitive Electrochemical Detection of Cancer-Associated Circulating microRNA in Serum Samples based on DNA Concatamers[J].Biosensors and Bioelectronics,2013,50:132-136.
[10]Carroll AE,Marrero DG,Downs SM.The HealthPia GlucoPackTMDiabetes Phone:a Usability Study[J].Diabetes Technology&Therapeutics,2007,9(2):158-164.
[11]Weber PC,Ohlendorf DH,Wendoloski JJ,et al.Structural Origins of High-Affinity Biotin Binding to Streptavidin[J].Science,1989,243(4887):85-88.
[12]Hermanson G T.The Chemistry of Reactive Groups[J].Biocon jugate techniques,2008,184:137-166.
[2]Schwarzenbach H,Hoon D S B,Pantel K.Cell-Free Nucleic Acids as Biomarkers in Cancer Patients[J].Nature Reviews Cancer,2011,11(6):426-437.
[3]Kolesnikova E V,Tamkovich S N,Bryzgunova O E,et al.Circulating DNA in the Blood of Gastric Cancer Patients[J].Annals of the New York Academy of Sciences,2008,1137(1):226-231.
[4]Kristensen L S,Hansen L L.PCR-based Methods for Detecting Single-Locus DNA Methylation Biomarkers in Cancer Diagnostics,Prognostics,and Response to Treatment[J].Clinical chemistry,2009,55(8):1471-1483.
[5]Bustin S A,Benes V,Garson J A,et al.The MIQE Guidelines:Minimum Information for Publication of Quantitative Real-time PCRExperiments[J].Clinical Chemistry,2009,55(4):611-622.
[6]Lane A A,Chabner B A.Histone Deacetylase Inhibitors in Cancer Therapy[J].Journal of Clinical Oncology,2009,27(32):5459-5468.
[7]Chen X,Hong CY,Lin JH,et al.Enzyme-free and Label-free Ultrasensitive Electrochemical Detection of Human Immunodeficiency Virus DNA in Biological Samples Based on Long-range Selfassembled DNA Nanostructures[J].Analytical Chemistry,2012,84:8277-8283.
[8]Chen X,Lin YH,Li J,et al.A Simple and Ultrasensitive Electrochemical DNA Biosensor Based on DNA Concatamers[J].Chemical Communicat ions,2011,47:12116-12118.
[9]Hong CY,Chen X,Liu T,et al.Ultrasensitive Electrochemical Detection of Cancer-Associated Circulating microRNA in Serum Samples based on DNA Concatamers[J].Biosensors and Bioelectronics,2013,50:132-136.
[10]Carroll AE,Marrero DG,Downs SM.The HealthPia GlucoPackTMDiabetes Phone:a Usability Study[J].Diabetes Technology&Therapeutics,2007,9(2):158-164.
[11]Weber PC,Ohlendorf DH,Wendoloski JJ,et al.Structural Origins of High-Affinity Biotin Binding to Streptavidin[J].Science,1989,243(4887):85-88.
[12]Hermanson G T.The Chemistry of Reactive Groups[J].Biocon jugate techniques,2008,184:137-166.