正交试验比较金银花中绿原酸、异绿原酸A的提取工艺
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摘要
目的:优化金银花的提取工艺,建立高效液相色谱法(HPLC)快捷检测绿原酸和异绿原酸A的方法。方法:以HPLC同时测定绿原酸、异绿原酸A的含量为指标,通过比较醇浓度、回流时间、超声功率、超声时间4因素对提取工艺的影响,优化超声波辅助醇法提取金银花的工艺。结果:以绿原酸、异绿原酸A的综合评分为指标,最佳提取工艺是60%乙醇超声(功率500 W)45 min,再回流提取1 h。结论:建立了准确灵敏的检测方法,优化的提取工艺经济、高效,能为生产提供理论依据。
Objective:To optimize the extraction process of Lonicerae flos and to establish an HPLC method to detect chlorogenic acid and isochlorogenic acid A rapidly.Methods:The content of chlorogenic acid and isochlorogenic acid A was simultaneously determined by HPLC and used as an indicator to compare the effect of the four factors(alcohol concentration,reflux time,ultrasonic power and ultrasonic time) on the extraction process,optimize the extraction process of Lonicerae flos by ultrasonic-assisted alcohol method.Results:Taking the comprehensive score of chlorogenic acid and isochlorogenic acid A as an indicator,the best extraction process was using 60% alcohol ultrasounding(power 500 W) for 45 min,then reflux extraction for 1 hour.Conclusions:An accurate and sensitive detection method is established,the optimized extraction process is economical and efficient,which can provide theoretical basis for production.
Objective:To optimize the extraction process of Lonicerae flos and to establish an HPLC method to detect chlorogenic acid and isochlorogenic acid A rapidly.Methods:The content of chlorogenic acid and isochlorogenic acid A was simultaneously determined by HPLC and used as an indicator to compare the effect of the four factors(alcohol concentration,reflux time,ultrasonic power and ultrasonic time) on the extraction process,optimize the extraction process of Lonicerae flos by ultrasonic-assisted alcohol method.Results:Taking the comprehensive score of chlorogenic acid and isochlorogenic acid A as an indicator,the best extraction process was using 60% alcohol ultrasounding(power 500 W) for 45 min,then reflux extraction for 1 hour.Conclusions:An accurate and sensitive detection method is established,the optimized extraction process is economical and efficient,which can provide theoretical basis for production.
引文
[1] 张瑾钰,陈一强,孔晋亮,等.绿原酸、异绿原酸对烟曲霉生物膜抑制作用的体外研究[J].中国感染与化疗杂志,2014,14(4):327.
[2] 周志娥,罗秋水,熊建华,等.绿原酸、异绿原酸A对大肠杆菌的抑菌机制[J].食品科技,2014,39(3):228.
[3] 侯彩平,何秋霞,韩利文,等.利用斑马鱼模型研究异绿原酸A的抗氧化和抗炎作用[C].中国毒理学会中药与天然药物毒理专业委员会第一次(2016年)学术交流大会,2016:75.
[4] 李祖晟,朱志安.二咖啡酰奎宁酸药理实验研究进展[J].医药综述,2004,10(4):249.
[5] 韩桂茹,张淑萍,安丽娜,等.HPLC法测定金银花及小儿咽扁颗粒中4种绿原酸[J].中成药,2013,35(3):517.
[6] 李淼,王永香,孟谨,等.HPLC法测定金银花中新绿原酸等8种成分的量[J].中草药,2014,45(7):1006.
[7] 华梅,毕玮,陈剑,等.金银花中异绿原酸A、B、C含量的HPLC测定方法研究[J].西部林业科学,2017,46(4):73.
[8] 王玥,杜守颖,吴清,等.金银花闪式提取工艺优选[J].中国实验方剂学杂志,2012,18(15):18.
[9] 闵春艳,游本刚,许琼明,等.正交试验比较金银花药材中绿原酸与木犀草甘的乙醇提取工艺[J].中成药,2011,33(10):1815.
[10] 府旗中,王伯初,许祥武.应用超声波法提取金银花中绿原酸[J].重庆大学学报(自然科学版),2007,30(1):123.
[11] 章运典,陈玉海.金银花中绿原酸提取工艺研究[J].中成药,2013,35(7):1564.
[2] 周志娥,罗秋水,熊建华,等.绿原酸、异绿原酸A对大肠杆菌的抑菌机制[J].食品科技,2014,39(3):228.
[3] 侯彩平,何秋霞,韩利文,等.利用斑马鱼模型研究异绿原酸A的抗氧化和抗炎作用[C].中国毒理学会中药与天然药物毒理专业委员会第一次(2016年)学术交流大会,2016:75.
[4] 李祖晟,朱志安.二咖啡酰奎宁酸药理实验研究进展[J].医药综述,2004,10(4):249.
[5] 韩桂茹,张淑萍,安丽娜,等.HPLC法测定金银花及小儿咽扁颗粒中4种绿原酸[J].中成药,2013,35(3):517.
[6] 李淼,王永香,孟谨,等.HPLC法测定金银花中新绿原酸等8种成分的量[J].中草药,2014,45(7):1006.
[7] 华梅,毕玮,陈剑,等.金银花中异绿原酸A、B、C含量的HPLC测定方法研究[J].西部林业科学,2017,46(4):73.
[8] 王玥,杜守颖,吴清,等.金银花闪式提取工艺优选[J].中国实验方剂学杂志,2012,18(15):18.
[9] 闵春艳,游本刚,许琼明,等.正交试验比较金银花药材中绿原酸与木犀草甘的乙醇提取工艺[J].中成药,2011,33(10):1815.
[10] 府旗中,王伯初,许祥武.应用超声波法提取金银花中绿原酸[J].重庆大学学报(自然科学版),2007,30(1):123.
[11] 章运典,陈玉海.金银花中绿原酸提取工艺研究[J].中成药,2013,35(7):1564.