家蚕不同龄期感染菜粉蝶微孢子虫的差异性分析
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摘要
为分析菜粉蝶微孢子虫对家蚕不同龄期的感染力差异,利用在云南省蒙自市收集的菜粉蝶,对其体内的微孢子虫进行分离、纯化与鉴定。通过叶面添食的方法,先后将菜粉蝶微孢子虫(约1.21×108粒)分别添给2、3、4、5龄起蚕各160头食用,随后正常饲养至上蔟结茧、化蛹、羽化。检验死蚕、不结茧蚕、死笼茧及蛾子中菜粉蝶微孢子虫的感染情况。综合统计分析菜粉蝶微孢子虫对各龄期家蚕的危害性和感染的差异性。结果表明:菜粉蝶微孢子虫对家蚕不同龄期的感染力存在显著性差异,随着家蚕龄期的增加,菜粉蝶微孢子虫的感染力逐渐降低。
In order to explore the diversity of infectivity of the silkworm larvae with different instars infected by the microsporidia isolated from Pieris rapae L,the cabbage butterfly were collected from Mengzi,Yunnan province. The microsporidian spores were isolated from the cabbage butterfly,purified and identified. The second,third,fourth,and fifth instar silkworm larvae(160 larvae/group) were respectively fed with the mulberry leaves which was treated with the microsporidian spores(approximately 1. 21 × 108spores). Then the silkworms were normally fed with the clean mulberry leaves until to cocooning,pupating and eclosion. The infection situation of dead silkworms,non-spinning silkworms,dead worm in cocoon and silkworm moth was researched. The diversity of infectivity of the silkworm larvae with different instars infected by the microsporidia was analyzed according to the statistical results. The results indicated that there was significant difference of the infection rate among the silkworm larvae with different instars infected by microsporidia isolated from Pieris rapae L. The infection rate of silkworm infected by microsporidia was gradually decreased with the increase of age.
In order to explore the diversity of infectivity of the silkworm larvae with different instars infected by the microsporidia isolated from Pieris rapae L,the cabbage butterfly were collected from Mengzi,Yunnan province. The microsporidian spores were isolated from the cabbage butterfly,purified and identified. The second,third,fourth,and fifth instar silkworm larvae(160 larvae/group) were respectively fed with the mulberry leaves which was treated with the microsporidian spores(approximately 1. 21 × 108spores). Then the silkworms were normally fed with the clean mulberry leaves until to cocooning,pupating and eclosion. The infection situation of dead silkworms,non-spinning silkworms,dead worm in cocoon and silkworm moth was researched. The diversity of infectivity of the silkworm larvae with different instars infected by the microsporidia was analyzed according to the statistical results. The results indicated that there was significant difference of the infection rate among the silkworm larvae with different instars infected by microsporidia isolated from Pieris rapae L. The infection rate of silkworm infected by microsporidia was gradually decreased with the increase of age.
引文
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[2]郑祥明,杨琼,方定坚,等.广东省昆虫微孢子虫资源调查及交叉感染的研究[J].蚕业科学,2003,29(4):380-383.
[3]廖模祥,刘吉平,张国平,等.野外昆虫来源微孢子虫与家蚕微粒子病发生的相关性研究[J].中国蚕业,2011(1):17-20.
[4]潘敏慧,崔红娟,万永继,等.菜粉蝶微孢子虫的多态性及对家蚕感染性的初步研究[J].四川蚕业,1999(3):10-11.
[5]杨琼.粉蝶属昆虫四种微孢子虫及对家蚕病原性研究[D].广州:华南农业大学,1999.
[6]郑祥明,杨琼,黄炳辉,等.菜粉蝶分离的微孢子虫对家蚕的病原性研究[J].广东蚕业,1997,31(2):41-44.
[7]Chen D R,Shen Z y,Zhu F,et al.Phylogenetic characterization of a microsporidium(Nosema sp.MPr)isolated from the Pieris rapae[J]Parasitology Research,2012,111(1):263-269.
[8]马育华.田间试验和统计方法(第2版)[M].北京:农业出版社,1993.
[9]陈世良,杨荣贵,高建华,等.菜粉蝶微孢子虫对家蚕胚种传染性与蚕桑质量影响研究[J].江苏农业科学,2015,43(8):241-243.
[10]刘吉平,曾玲.微孢子虫生物多样性研究的评述[J].昆虫知识,2006,43(2):153-158.
[11]陈世良,高翔,张金祥,等.云南菜粉蝶微孢子虫感染家蚕的病原性研究[J].西南农业学报,2015,29(2):913-917.