27种中草药的黄嘌呤氧化酶抑制活性筛选
|
摘要
目的研究27种常见中药材甲醇提取物对黄嘌呤氧化酶的抑制活性。方法以别嘌醇为阳性对照,采用全波长酶标仪测定反应体系的吸光度,测定波长为295 nm,通过Graph Pad Prism 5软件计算抑制率。结果 27种中药甲醇提取物均对黄嘌呤氧化酶显示不同程度的抑制作用,其中6种中药(丹参、野菊花、牡丹皮、川芎、当归、远志)对黄嘌呤氧化酶具有较好的抑制作用,其IC_(50)分别为91.2、39.5、87.7、11.8、54.6、94.1μg/ml(阳性别嘌醇的IC50为6.4μg/ml)。结论该研究首次发现牡丹皮、川芎和远志具有显著的黄嘌呤氧化酶抑制活性,此结果可为揭示这些中药的抗痛风作用提供科学数据,同时亦为其进一步开发利用提供科学参考。
引文
[1]Enroth C,Eger B T,Okamoto K,et al.Crystal structures of bovine milk xanthine dehydrogenase and xanthine oxidase:structure-based mechanism of conversion[J].Proc Natl Acad Sci,2000,97(20):10723.
[2]焉翠蔚,赵庆娅,张珞,等.海带提取物对黄嘌呤氧化酶的抑制作用[J].中国海洋大学学报(自然科学版),2013,43(12):36.
[3]Kong L D,Cai Y,Huang W W,et al.Inhibition of xanthine oxidase by some Chinese medicinal plants used to treat gout[J].J Ethnopharmacol,2000,73(1):199.
[4]Liu X,Chen R,Shang Y,et al.Superoxide radicals scavenging and xanthine oxidase inhibitory activity of magnesium lithospermate B from Salvia miltiorrhiza[J].J Enzyme Inhib Med Chem,2009,24(3):663.
[5]中华医学会内分泌学分会.高尿酸血症和痛风治疗的中国专家共识[J].中华内分泌代谢杂志,2013,29(11):913.
[6]Zhu Y,Pandya B J,Choi H K.Comorbidities of gout and hyperuricemia in the US general population:NHANES 2007-2008[J].Am J Med,2012,125(7):679.
[7]Dogan A,Yarlioglues M,Kaya MG,et al.Effect of long-term and high-dose allopurinol therapy on endothelial function in normotensive diabetic patients[J].Blood Press,2011,20(3):182.
[8]Nguyen M T,Awale S,Tezuka Y,et al.Xanthine oxidase inhibitors from the flowers of Chrysanthemum sinense[J].Planta Med,2006,72(1):46.
[9]陈东银,李立新.痛风合理用药与食疗[M].北京:金盾出版社,2014.
[2]焉翠蔚,赵庆娅,张珞,等.海带提取物对黄嘌呤氧化酶的抑制作用[J].中国海洋大学学报(自然科学版),2013,43(12):36.
[3]Kong L D,Cai Y,Huang W W,et al.Inhibition of xanthine oxidase by some Chinese medicinal plants used to treat gout[J].J Ethnopharmacol,2000,73(1):199.
[4]Liu X,Chen R,Shang Y,et al.Superoxide radicals scavenging and xanthine oxidase inhibitory activity of magnesium lithospermate B from Salvia miltiorrhiza[J].J Enzyme Inhib Med Chem,2009,24(3):663.
[5]中华医学会内分泌学分会.高尿酸血症和痛风治疗的中国专家共识[J].中华内分泌代谢杂志,2013,29(11):913.
[6]Zhu Y,Pandya B J,Choi H K.Comorbidities of gout and hyperuricemia in the US general population:NHANES 2007-2008[J].Am J Med,2012,125(7):679.
[7]Dogan A,Yarlioglues M,Kaya MG,et al.Effect of long-term and high-dose allopurinol therapy on endothelial function in normotensive diabetic patients[J].Blood Press,2011,20(3):182.
[8]Nguyen M T,Awale S,Tezuka Y,et al.Xanthine oxidase inhibitors from the flowers of Chrysanthemum sinense[J].Planta Med,2006,72(1):46.
[9]陈东银,李立新.痛风合理用药与食疗[M].北京:金盾出版社,2014.