苦豆子不同提取物对小鼠急性毒性的“谱-毒”关系研究
|
摘要
目的建立苦豆子提取物HPLC化学指纹图谱,测定苦豆子在不同提取物对小鼠的半数致死量(LD_(50)),分析其"谱-毒"关系。方法采用75%乙醇回流法(ER)、水煎煮法(WD)、75%乙醇超声法(EU)和水超声法(WU)分别制备苦豆子提取物,并建立其HPLC指纹图谱,测定不同提取物的LD_(50);运用指纹图谱相似度评价系统软件分析不同提取方法下苦豆子提取物化学成分与LD_(50)之间的关系。结果 ER、WD、EU、WU 4种提取方法制备的苦豆子提取物LD_(50)分别为38.397、24.994、18.536、19.957g/kg;苦豆子提取物对小鼠脏器的眼观病变主要表现在肝、肾,其中以ER提取物毒性最大。苦豆子提取物HPLC 10个共有峰可分为2类,第4、10号峰及氧化苦参碱、氧化槐果碱与LD_(50)呈显著负相关。结论构建了苦豆子"谱-毒"关系分析方法,其未鉴定出的第4、10号峰及氧化苦参碱和氧化槐果碱为毒性反应的主要化学成分。
Objective To establish the chemical fingerprint of Sophora alopecuroides extracts based on high performance liquid chromatography(HPLC), and determine the LD_(50) of different extracts of S. alopecuroides to analyze its "spectrum toxicity"relationship. Methods A series of extracts were prepared by 75% ethanol reflux(ER), water decoction(WD), 75% ethanol ultrasound(EU) and water ultrasound(WU), and their fingerprints were established to determine the acute toxicity LD_(50) of different extracts. The relationship between chemical composition and acute toxicity LD_(50) of S. alopecuroides extracts were studied by means of fingerprint similarity evaluation system. Results The LD_(50) of ER, WD, EU, and WU extracts were 38.397, 24.994, 18.536, and 19.957 g/kg,respectively. The ocular lesions of mice viscera were mainly manifested in liver and kidney, and the toxicity of ER extracts was the greatest. The 10 common peaks of S. alopecuroides extracts can be divided into two categories; Peaks 4 and 10, oxymatrine and sophocarpidine were negatively correlated with acute toxicity LD_(50). Conclusion The spectral toxicity relationship analysis method of S. alopecuroides was constructed. The unidentified peaks 4, 10 and oxymatrine and sophocarpidine were the main chemical components of the toxicity reaction, which laid a good foundation for clinical application and scientific and rational development of S.alopecuroides.
Objective To establish the chemical fingerprint of Sophora alopecuroides extracts based on high performance liquid chromatography(HPLC), and determine the LD_(50) of different extracts of S. alopecuroides to analyze its "spectrum toxicity"relationship. Methods A series of extracts were prepared by 75% ethanol reflux(ER), water decoction(WD), 75% ethanol ultrasound(EU) and water ultrasound(WU), and their fingerprints were established to determine the acute toxicity LD_(50) of different extracts. The relationship between chemical composition and acute toxicity LD_(50) of S. alopecuroides extracts were studied by means of fingerprint similarity evaluation system. Results The LD_(50) of ER, WD, EU, and WU extracts were 38.397, 24.994, 18.536, and 19.957 g/kg,respectively. The ocular lesions of mice viscera were mainly manifested in liver and kidney, and the toxicity of ER extracts was the greatest. The 10 common peaks of S. alopecuroides extracts can be divided into two categories; Peaks 4 and 10, oxymatrine and sophocarpidine were negatively correlated with acute toxicity LD_(50). Conclusion The spectral toxicity relationship analysis method of S. alopecuroides was constructed. The unidentified peaks 4, 10 and oxymatrine and sophocarpidine were the main chemical components of the toxicity reaction, which laid a good foundation for clinical application and scientific and rational development of S.alopecuroides.
引文
[1]木胡牙提,刘志强,马依彤,等.临床型医学硕士研究生培养中面临的难点及解决对策[J].新疆医学,2008,38(5):129-131.
[2]李艳艳,冯俊涛,张兴,等.苦豆子化学成分及其生物活性研究进展[J].西北农业学报,2005,14(2):133-136.
[3]郝伟亮,孟根达来,解红霞.苦豆子的化学成分及药理作用研究进展[J].中国药房,2016,27(13):1848-1850.
[4]曹楠楠,陈香荣,吴艳.苦豆子多糖的超声波提取工艺优化及理化性质研究[J].现代食品科技,2014,30(2):209-215.
[5]刘军,熊元君,李勇,等.苦豆子研究近况[J].新疆中医药,2006,24(6):60-63.
[6]蔡艳.苦参素药理作用研究进展[J].实用中医药杂志,2016,32(4):387-389.
[7]王新峰,李磊,韩国柱.苦参素药理作用的研究进展[J].医药导报,2005,24(6):483-485.
[8]吴芳妮,张芊,张春雷.苦参在T细胞介导的皮肤良恶性疾病中的应用进展[J].中国皮肤性病学杂志,2016,30(8):847-849.
[9]新疆维吾尔自治区维吾尔药材标准[S].2010.
[10]杨舒淳,钟晓铃,刘姝君,等.不同炮制方法对苦豆子毒性的影响[J].新疆中医药,2008,26(5):34-35.
[11]孙瑞元.简捷实用的半数致死量综合计算法[J].药学学报,1963(2):65-74.
[12]吕莉莉,赵红,李世民,等.不同提取方式对益母草小鼠急性毒性及毒靶器官的影响[J].中国药物警戒,2015,12(12):705-710.
[13]薛峰,李春娜,李朋收,等.超声提取在中药化学成分提取中的应用[J].中国实验方剂学杂志,2014,20(18):231-234.
[14]万水昌,王志祥,乐龙,等.超声提取技术在中药及天然产物提取中的应用[J].西北药学杂志,2008,23(1):60-62.
[15]张立军,李芸,戴海蓉,等.高乌头不同溶剂萃取部分急性毒性的谱-毒关系研究[J].中国临床药理学杂志,2018,34(4):470-474.
[16]林艳,肖榕,尹林子,等.基于粗糙集理论构建“谱毒”关系研究何首乌特异质肝毒性物质基础[J].中国中药杂志,2019,44(3):509-517.
[17]陈申,刘宇,强哲,等.苦豆子总碱对小鼠急性毒性试验研究[J].中兽医医药杂志,2013,32(2):34-36.
[2]李艳艳,冯俊涛,张兴,等.苦豆子化学成分及其生物活性研究进展[J].西北农业学报,2005,14(2):133-136.
[3]郝伟亮,孟根达来,解红霞.苦豆子的化学成分及药理作用研究进展[J].中国药房,2016,27(13):1848-1850.
[4]曹楠楠,陈香荣,吴艳.苦豆子多糖的超声波提取工艺优化及理化性质研究[J].现代食品科技,2014,30(2):209-215.
[5]刘军,熊元君,李勇,等.苦豆子研究近况[J].新疆中医药,2006,24(6):60-63.
[6]蔡艳.苦参素药理作用研究进展[J].实用中医药杂志,2016,32(4):387-389.
[7]王新峰,李磊,韩国柱.苦参素药理作用的研究进展[J].医药导报,2005,24(6):483-485.
[8]吴芳妮,张芊,张春雷.苦参在T细胞介导的皮肤良恶性疾病中的应用进展[J].中国皮肤性病学杂志,2016,30(8):847-849.
[9]新疆维吾尔自治区维吾尔药材标准[S].2010.
[10]杨舒淳,钟晓铃,刘姝君,等.不同炮制方法对苦豆子毒性的影响[J].新疆中医药,2008,26(5):34-35.
[11]孙瑞元.简捷实用的半数致死量综合计算法[J].药学学报,1963(2):65-74.
[12]吕莉莉,赵红,李世民,等.不同提取方式对益母草小鼠急性毒性及毒靶器官的影响[J].中国药物警戒,2015,12(12):705-710.
[13]薛峰,李春娜,李朋收,等.超声提取在中药化学成分提取中的应用[J].中国实验方剂学杂志,2014,20(18):231-234.
[14]万水昌,王志祥,乐龙,等.超声提取技术在中药及天然产物提取中的应用[J].西北药学杂志,2008,23(1):60-62.
[15]张立军,李芸,戴海蓉,等.高乌头不同溶剂萃取部分急性毒性的谱-毒关系研究[J].中国临床药理学杂志,2018,34(4):470-474.
[16]林艳,肖榕,尹林子,等.基于粗糙集理论构建“谱毒”关系研究何首乌特异质肝毒性物质基础[J].中国中药杂志,2019,44(3):509-517.
[17]陈申,刘宇,强哲,等.苦豆子总碱对小鼠急性毒性试验研究[J].中兽医医药杂志,2013,32(2):34-36.