摘要
为了提高石油污染土壤的生物降解效率,以甲基丙烯酸丁酯制备的树脂为载体吸附石油降解菌进行生物降解,利用GC-MS"指纹图谱"对TPH(total petroleum hydrocarbon,总石油烃)的降解程度及生物演化特性进行分析.结果表明:树脂吸附菌剂后TPH的半衰期为24.23 d,比单纯菌剂降解缩短7.28 d;其中正构烷烃的降解性能表明,高碳数高达92.03%,中、低碳数皆超过75%,高于TPH的56.67%,较好地解释了TPH降解率比单纯菌剂提高的内在原因;在演化特征方面,正构烷烃的生物演化参数———w(∑C_(21))/w(∑C_(22+))(C_(21-)、C_(22+)分别指C_(14)~C_(21)和C_(22)~C_(39))、姥植比[w(pr)/w(ph)]和OEP(奇/偶碳数质量比)分别为0.643 3、0.486 5和1.111 9,均高于单纯菌剂演化程度.研究显示,树脂吸附菌剂后对较难降解的偶数碳正构烷烃和类异戊二烯烃类物质的氧化还原能力增强,从生物演化的角度有力地佐证了降解的优势所在.
In order to improve the biodegradation efficiency of petroleum-contaminated soil,biological degradation was studied with oildegradation bacteria adsorbed on resin(ODB-AOR)that was made of butyl methacrylate.The degradation of TPH(Total Petroleum Hydrocarbon)and the biological transformation characteristics were analyzed through a GC-MS fingerprint.The results showed that the half-life of TPH in ODB-AOR was 24.23 d,which was 7.28 days shorter than using the pure bacterium.The degradation rate of alkanes with a high carbon number reached 92.03%,and rate of alkanes with low/medium numbers was greater than 75%,which was significantly higher than the degradation rate of TPH(56.67%).It's why the degradation rate of TPH was greater than pure bacterium.The study on transformation characteristics of ODB-AOR showed that biological marker ratio of normal alkanes of high carbon/low carbon,pristane/phytane,and odd/even were 0.6433,0.4865 and 1.1119,respectively,which were all higher than the degradation of the pure bacterium.The results indicated that ODB-AOR could significantly enhance the ability of oxidation-reduction for n-alkanes in even-numbered carbon and isoprenoid hydrocarbon,which proves the advantages of the degradation on the basis of biological evolution.
引文
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