壳聚糖接枝物改性胶原蛋白海绵状敷料的制备与表征
|
摘要
以胶原蛋白(Col)为载体,壳聚糖(CS)和羟丙甲纤维素邻苯二甲酸酯(HPMCP)的接枝共聚物CS-gHPMCP为性能改良剂,采用冷冻干燥工艺制备不同CS-g-HPMCP含量的Col/CS-g-HPMCP复合海绵状敷料,并利用扫描电子显微镜对其多孔结构进行表征,探讨不同CS-g-HPMCP含量对复合海绵状敷料的溶胀率、溶失率、透湿量以及力学性能的影响。结果表明,随着CS-g-HPMCP含量的增加,复合海绵状敷料的溶胀率总体上增大,溶失率总体上降低,透湿量、拉伸强度和断裂伸长率先升高后降低。当CS-g-HPMCP质量分数为60%时,复合海绵状敷料的溶胀率为313.7%,溶失率为27.4%,透湿量为5 282.3g/(m2·d),拉伸强度和断裂伸长率最高,分别为45.6MPa和23.9%,其各项性能优异,是具有应用潜力的海绵状医用敷料。
Using collagen(Col) as carrier material and the graft copolymer CS-g-HPMCP of chitosan(CS) and hydroxypropyl phthalate(HPMCP) as the performance modi?er,Col/CS-g-HPMCP composite sponge dressings with different CS-g-HPMCP contents were prepared by freeze-drying process and their polyporous structures were characterized by SEM. The effects of CS-g-HPMCP contents on the swelling ratio,dissolution ratio,moisture permeability and mechanical properties of the sponge dressings were investigated. The results show that with the increase of CS-g-HPMCP content,the swelling ratio of the sponge dressing increases in total,the dissolution ratio decreases in total,the moisture permeability,tensile strength and elongation at break all ?rst increase and then decrease. When the mass fraction of CS-g-HPMCP is 60%,the swelling ratio is 313.7%,dissolution rate is 27.4%,moisture permeability is 5 282.3 g/(m2· d),and the tensile strength and elongation at break are maximum,which are 45.6 MPa and 23.9%respectively. These indicate that the sponge dressing has excellent performances and is a spongy medical dressing with potential application.
Using collagen(Col) as carrier material and the graft copolymer CS-g-HPMCP of chitosan(CS) and hydroxypropyl phthalate(HPMCP) as the performance modi?er,Col/CS-g-HPMCP composite sponge dressings with different CS-g-HPMCP contents were prepared by freeze-drying process and their polyporous structures were characterized by SEM. The effects of CS-g-HPMCP contents on the swelling ratio,dissolution ratio,moisture permeability and mechanical properties of the sponge dressings were investigated. The results show that with the increase of CS-g-HPMCP content,the swelling ratio of the sponge dressing increases in total,the dissolution ratio decreases in total,the moisture permeability,tensile strength and elongation at break all ?rst increase and then decrease. When the mass fraction of CS-g-HPMCP is 60%,the swelling ratio is 313.7%,dissolution rate is 27.4%,moisture permeability is 5 282.3 g/(m2· d),and the tensile strength and elongation at break are maximum,which are 45.6 MPa and 23.9%respectively. These indicate that the sponge dressing has excellent performances and is a spongy medical dressing with potential application.
引文
[1]梁飞,左红梅.胶原蛋白肽的性质、应用及发展前景的研究综述[J].明胶科学与技术,2014,34(3):109-115.Liang Fei,Zuo Hongmei.A review on the properties,applications and prospectof collagen peptide[J].The Science and Technology of Gelatin,2014,34(3):109-115.
[2]赵景华,吴兆明,丁宇宁,等.胶原蛋白的化学改性方法及其应用的研究进展[J].渔业研究,2017,39(2):147-156.Zhao Jinghua,Wu Zhaoming,Ding Yuning,et al.Research progress on chemical modification and application of collagen[J].Journal of Fisheries Research,2017,39(2):147-156.
[3]王浩.壳聚糖的改性研究进展及其应用[J].成都纺织高等专科学校学报,2017,34(1):187-194.Wang Hao.Chitosan modification research progress and application[J].Journal of Chengdu Textile College,2017,34(1):187-194.
[4]张云凤,杜国婷,王煜.胶原蛋白的改性方法概述[J].化工技术与开发,2015,44(6):46-50.Zhang Yunfeng,Du Guoting,Wang Yu.Modification methods of collagen[J].Technology&Development of Chemical Industry,2015,44(6):46-50.
[5]Zhao L L,Li X,Zhao J Q,et al.A novel smart injectable hydrogel prepared by microbial transglutaminase and human-like collagen:Its characterization and biocompatibility[J].Mater Sci Eng C,2016,68:317-326.
[6]Chang L,Liu X L,Di F D,et al.The efficiency of magnetic hyperthermia and in vivo histocompatibility for human-like collagen protein-coated magnetic nanoparticles[J].Int J Nanomedicine,2016,11:1 175-1 185.
[7]卢亢,陈泽楚,李婷,等.壳聚糖的制备及其抑菌性能的研究[J].离子交换与吸附,2015,31(2):1 705-1 708.Lu Kang,Chen Zechu,Li Ting,et al.Preparation and antibacterial properties of chitosan[J].Ion Exchange and Adsorption,2015,31(2):1 705-1 708.
[8]蔚鑫鑫,刘艳,吴光旭.小龙虾壳中的甲壳素的提取及壳聚糖的制备[J].湖北农业科学,2013,52(13):3 120-3 123.Yu Xinxin,Liu Yan,Wu Guangxu.Extraction of chitin and preparation of chitosan from procambarus clarkii shell[J].Hubei Agricultural Sciences,2013,52(13):3 120-3 123.
[9]Mirazul I,Vytautas C,Chaoliang,et al.Functional fabrication of recombinant human collagen-phosphorylcholine hydrogels[J].Acta Biomaterialia,2015,12:70-80.
[10]Islam S,Bhuiyan M A R,Islam M N.Chitin and chitosan:Structure,properties and application in biomedical engineering[J].Journal of Polymers&the Environment,2017,25:854-866.
[11]Xiao Z,Tian T,Hu J,et al.Preparation and characterization of chitosan nanoparticles as the delivery system for tuberose fragrance[J].Flavour&Fragrance Journal,2014,29(1):22-34.
[12]Walters B D,Stegemann J P.Strategies for directing the structure and function of three-dimensional collagen biomaterials across length scales[J].Acta Biomaterialia,2014;10(4):1 488-1 501.
[13]何静.胶原蛋白在生物医药工程中的应用研究进展[J].河南科技,2015(10):104-105.He Jing.Progress in the application of collagen in biomedical engineering[J].Journal of Henan Science and Technology,2015(10):104-105.
[14]Singh B,Maharjan S,Jiang T,et al.Attuning hydroxypropyl methylcellulose phthalate to oral delivery vehicle for effective and selective delivery of protein vaccine in ileum[J].Biomaterials,2015,59:144-159.
[15]Wang M,Xu L,Li C,et al.Antipolyelectrolyte swelling of amphiphilic hydroxypropyl methylcellulose phthalate gels[J].Colloids and Surfaces A:Physicochemical and Engineering Aspects,2010,356(1-3):89-96.
[2]赵景华,吴兆明,丁宇宁,等.胶原蛋白的化学改性方法及其应用的研究进展[J].渔业研究,2017,39(2):147-156.Zhao Jinghua,Wu Zhaoming,Ding Yuning,et al.Research progress on chemical modification and application of collagen[J].Journal of Fisheries Research,2017,39(2):147-156.
[3]王浩.壳聚糖的改性研究进展及其应用[J].成都纺织高等专科学校学报,2017,34(1):187-194.Wang Hao.Chitosan modification research progress and application[J].Journal of Chengdu Textile College,2017,34(1):187-194.
[4]张云凤,杜国婷,王煜.胶原蛋白的改性方法概述[J].化工技术与开发,2015,44(6):46-50.Zhang Yunfeng,Du Guoting,Wang Yu.Modification methods of collagen[J].Technology&Development of Chemical Industry,2015,44(6):46-50.
[5]Zhao L L,Li X,Zhao J Q,et al.A novel smart injectable hydrogel prepared by microbial transglutaminase and human-like collagen:Its characterization and biocompatibility[J].Mater Sci Eng C,2016,68:317-326.
[6]Chang L,Liu X L,Di F D,et al.The efficiency of magnetic hyperthermia and in vivo histocompatibility for human-like collagen protein-coated magnetic nanoparticles[J].Int J Nanomedicine,2016,11:1 175-1 185.
[7]卢亢,陈泽楚,李婷,等.壳聚糖的制备及其抑菌性能的研究[J].离子交换与吸附,2015,31(2):1 705-1 708.Lu Kang,Chen Zechu,Li Ting,et al.Preparation and antibacterial properties of chitosan[J].Ion Exchange and Adsorption,2015,31(2):1 705-1 708.
[8]蔚鑫鑫,刘艳,吴光旭.小龙虾壳中的甲壳素的提取及壳聚糖的制备[J].湖北农业科学,2013,52(13):3 120-3 123.Yu Xinxin,Liu Yan,Wu Guangxu.Extraction of chitin and preparation of chitosan from procambarus clarkii shell[J].Hubei Agricultural Sciences,2013,52(13):3 120-3 123.
[9]Mirazul I,Vytautas C,Chaoliang,et al.Functional fabrication of recombinant human collagen-phosphorylcholine hydrogels[J].Acta Biomaterialia,2015,12:70-80.
[10]Islam S,Bhuiyan M A R,Islam M N.Chitin and chitosan:Structure,properties and application in biomedical engineering[J].Journal of Polymers&the Environment,2017,25:854-866.
[11]Xiao Z,Tian T,Hu J,et al.Preparation and characterization of chitosan nanoparticles as the delivery system for tuberose fragrance[J].Flavour&Fragrance Journal,2014,29(1):22-34.
[12]Walters B D,Stegemann J P.Strategies for directing the structure and function of three-dimensional collagen biomaterials across length scales[J].Acta Biomaterialia,2014;10(4):1 488-1 501.
[13]何静.胶原蛋白在生物医药工程中的应用研究进展[J].河南科技,2015(10):104-105.He Jing.Progress in the application of collagen in biomedical engineering[J].Journal of Henan Science and Technology,2015(10):104-105.
[14]Singh B,Maharjan S,Jiang T,et al.Attuning hydroxypropyl methylcellulose phthalate to oral delivery vehicle for effective and selective delivery of protein vaccine in ileum[J].Biomaterials,2015,59:144-159.
[15]Wang M,Xu L,Li C,et al.Antipolyelectrolyte swelling of amphiphilic hydroxypropyl methylcellulose phthalate gels[J].Colloids and Surfaces A:Physicochemical and Engineering Aspects,2010,356(1-3):89-96.