西北地区和尚头小麦遗传多样性及农艺性状的关联分析
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摘要
为了解西北地区和尚头小麦种质资源的遗传特性及主要农艺性状特征,采用43份不同来源的和尚头小麦种质材料,在均匀分布于小麦21条染色体上的150个SSR(simple sequence repeats)位点上检测遗传多样性,同时对19个农艺性状在两个试验点进行表型鉴定,并采用GLM(general linear model)模型进行分子标记与表型性状的关联分析。结果表明,19个农艺性状中数量性状遗传多样性明显高于质量性状,除壳色外,其他性状之间均存在不同程度的相关性。利用45对具有多态性的SSR标记共检测出151个等位变异,各标记等位位点变化范围为2～6,平均为3.36个,PIC(polymorphism information content)值变异范围为0.044～0.771。群体遗传结构分析将43份材料划分为8个亚群。关联分析发现11个SSR标记与农艺性状显著关联,单个标记对表型变异的解释率为8.89%～24.74%,这些标记可为特色小麦分子标记辅助选择育种提供理论参考。
Key aims of this study were:1)to better understand the genetic structure of the Heshangtou wheat landrace from northwestern China,and 2)to explore the link between genetic markers and agronomic traits.A collection of 43 germplasm lines from different regions in northwest China was genetically characterized using150 simple sequence repeat(SSR)marker loci,which were evenly distributed across the 21 chromosomes of wheat.Concurrently,19 agronomic traits were recorded for the same germplasm lines at two test sites,and a general linear model(GLM)was used to analyze the association between molecular markers and phenotypic traits.A total of 151 allelic variants were detected using 45 pairs of polymorphic SSR markers.The number of alleles in each marker ranged from 2to 6,with an average of 3.36,and the polymorphic information content(PIC)varied from 0.044 to 0.771.It was found that the genetic diversity among the 19 agronomic traits assessed in this way was significantly higher than that of quality traits.The agronomic traits(except for shell color)exhibited varying degrees of correlation.Population genetic structure analysis classified the 43 germplasm lines into 8subgroups.Correlation analysis showed that 11 SSR markers were significantly associated with particular agronomic traits,and the extent to which phenotypic variation was explained by single markers ranged from 8.89%-24.74%.Some of the markers identified may be useful for marker-assisted selection in wheat breeding.
Key aims of this study were:1)to better understand the genetic structure of the Heshangtou wheat landrace from northwestern China,and 2)to explore the link between genetic markers and agronomic traits.A collection of 43 germplasm lines from different regions in northwest China was genetically characterized using150 simple sequence repeat(SSR)marker loci,which were evenly distributed across the 21 chromosomes of wheat.Concurrently,19 agronomic traits were recorded for the same germplasm lines at two test sites,and a general linear model(GLM)was used to analyze the association between molecular markers and phenotypic traits.A total of 151 allelic variants were detected using 45 pairs of polymorphic SSR markers.The number of alleles in each marker ranged from 2to 6,with an average of 3.36,and the polymorphic information content(PIC)varied from 0.044 to 0.771.It was found that the genetic diversity among the 19 agronomic traits assessed in this way was significantly higher than that of quality traits.The agronomic traits(except for shell color)exhibited varying degrees of correlation.Population genetic structure analysis classified the 43 germplasm lines into 8subgroups.Correlation analysis showed that 11 SSR markers were significantly associated with particular agronomic traits,and the extent to which phenotypic variation was explained by single markers ranged from 8.89%-24.74%.Some of the markers identified may be useful for marker-assisted selection in wheat breeding.
引文
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[2] Fu X Y,Fu Y W,Liu G R.Advanced research on genetic diversity of wheat.Acta Agriculturae Boreali-Sinica,2008,23(B10):142-145.傅晓艺,付艺伟,刘桂茹.小麦遗传多样性的研究进展.华北农学报,2008,23(B10):142-145.
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[8] Fan L P,Wu P H,Wang L P,et al.Analysis of genetic diversity in Sea Island cotton based on genetic and phenotypic traits.Journal of Plant Genetic Resources,2016,17(2):197-208.范李萍,吴鹏昊,王莉萍,等.基于遗传和表型特征的海岛棉遗传多样性分析.植物遗传资源学报,2016,17(2):197-208.
[9] Qu Y,Zhou Y,Wang Z,et al.Analysis of genetic diversity and structure of tartary buckwheat resources from production regions.Scientia Agricultura Sinica,2016,49(11):2049-2062.屈洋,周瑜,王钊,等.苦荞产区种质资源遗传多样性和遗传结构分析.中国农业科学,2016,49(11):2049-2062.
[10] Chen S Y,Ma X,Zhang X Q,et al.The transferability of SSR and EST-SSR markers of different origins in Elymus and Roegneriain the Triticeae(Poaceae).Acta Prataculturae Sinica,2016,25(2):132-140.陈仕勇,马啸,张新全,等.不同来源SSR和EST-SSR在披碱草属和鹅观草属物种中的通用性分析.草业学报,2016,25(2):132-140.
[11] Diwan N,Cregan P.Automated sizing of fluorescent-labeled simple sequence repeat(SSR)markers to assay genetic variation in soybean.Theoretical and Applied Genetics,1997,95(5):723-733.
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[13] Li Z L,Hu L,Wang H W,et al.Genetic diversity of Henan wheat landreces with the same name detected by SSR markers.Journal of Triticeae Crops,2016,36(5):564-570.李正玲,胡琳,王会伟,等.河南省同名小麦地方品种SSR遗传多样性分析.麦类作物学报,2016,36(5):564-570.
[14] Yu M Z,Zhang C,Meng J J,et al.Genetic diversity and population structure analysis of winter wheat in east Gansu Province.Molecular Plant Breeding,2016,14(11):3232-3239.于明寨,张成,孟建军,等.陇东地区冬小麦遗传多样性及群体结构分析.分子植物育种,2016,14(11):3232-3239.
[15] Li X Q,Gao Y H,Liu Y,et al.The genetic diversity of 9populations of dry-desert Agropyron mongolicum collected in northern China.Acta Prataculturae Sinica,2016,25(3):77-85.李晓全,高有汉,刘扬,等.我国北方9份旱生-沙生植物蒙古冰草遗传多样性研究.草业学报,2016,25(3):77-85.
[16] Hao C Y,Wang L F,Ge H M,et al.Genetic diversity and linkage disequilibrium in chinese bread wheat(Triticum aestivum L.)revealed by SSR markers.PLoS One,2011,6(2):e17279.
[17] Zhang D D,Bai G H,Zhu C S,et al.Genetic diversity,population structure,and linkage disequilibrium in U.S.elite winter wheat.The Plant Genome Journal,2010,3(2):117-127.
[18] Breseghello F,Sorrells M E.Associatino mapping of kernel size and milling quality in wheat(Triticum aestivum L.)cultivars.Genetics,2006,172(2):1165-1177.
[19] Zhao Y,Wang H,Chen W,et al.Genetic structure,linkage disequilibrium and association mapping of Verticillium wilt resistance in elite cotton(Gossypium hirsutum L.)germplasm population.PLoS One,2014,9(1):e86308.
[20] Ma X L,Li B C,Meng Y X,et al.Identification of 1BL/1RS translocation lines and analysis of gluten cooking features of wheat(Triticum aestivum)germplasms in Gansu Province.Journal of Agricultural Biotechnology,2015,23(6):747-754.马小乐,李葆春,孟亚雄,等.甘肃小麦品种1BL/1RS易位系鉴定及面筋熟制特性分析.农业生物技术学报,2015,23(6):747-754.
[21] Wang S H.Analysis of quality traits for major high-quality wheat in Gansu Province and QTLs mapping of quality traits from landrace Heshangtou.Lanzhou:Gansu Agricultural University,2014.王世红.甘肃省主要优质小麦品种品质性状分析及和尚头品质性状QTL定位.兰州:甘肃农业大学,2014.
[22] Zhang K,Chen N L,Gu Q Y,et al.Respinses of gas exchanges characteristics and biomass accumulation and allocation of spring wheat with different drought tolerance to water and nitrogen treatments.Journal of Nuclear Agricultural Sciences,2016,30(4):797-804.张凯,陈年来,顾群英,等.不同抗旱性小麦气体交换特性和生物量积累与分配对水氮的响应.核农学报,2016,30(4):797-804.
[23] Du J Q,Gao Y G.Effects of water supply on the water use efficiency and grain yield of new and old two spring wheat.Biotech World,2013,(8):37-38.杜京旗,高勇钢.水分供给对新老两个春小麦品种水分利用效率及籽实产量的影响.生物技术世界,2013,(8):37-38.
[24] Lian L Y,Liu Y A,Dou Q W,et al.Differences of nitrogen efficiencies between spring wheat varieties under water stress.Acta Agriculturae Boreali-occidentalis Sinica,2011,20(5):64-68.连利叶,刘永安,窦全文,等.水分胁迫下不同春小麦品种氮效率的差异.西北农业学报,2011,20(5):64-68.
[25] Gao T P,An L Z,Feng H Y.Effects of enhanced UV-B irradiance and drought stress on the growth,production,and biomass of spring wheat.Scientia Agricultura Sinica,2009,42(6):1933-1940.高天鹏,安黎哲,冯虎元.增强UV-B辐射和干旱对不同品种春小麦生长、产量和生物量的影响.中国农业科学,2009,42(6):1933-1940.
[26] Li L H,Li X Q,Yang X M.Descriptors and data standard for wheat.Beijing:China Agriculture Press,2006:3-7.李立会,李秀全,杨欣明.小麦种质资源描述规范和数据标准.北京:中国农业出版社,2006:3-7.
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