红肉火龙果色素提取工艺优化及其抗氧化研究
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摘要
为了探索红肉火龙果色素的超声提取最佳工艺及其体外抗氧化作用。采用星点设计优化红肉火龙果色素的超声提取工艺。在单因素试验基础上,以红肉火龙果果肉色素吸光度值优化最佳提取工艺:以水为提取溶剂,超声提取温度为42℃、提取时间问38 min、液料比为60︰1,红肉火龙果果肉色素的吸光值为0.507。红肉火龙果色素对DPPH自由基有较强的清除作用,并与红肉火龙果色素浓度呈一定的量效关系,结果显示红肉火龙果色素较好的抗氧化活性,作为一种天然的抗氧化剂具有较好的应用前景。
To study the ultrasonic extraction process of Pigments from Flesh of Red-fleshed Pitaya and antioxidant effect in vitro. The ultrasonic extraction technology of Pigments from Flesh of Red-fleshed Pitaya was optimized by using response surface.On the basis of single factor experiment, pitaya pulp pigment with red flesh absorbance value as a response surface for the optimization of extraction process. With water as extraction solvent, ultrasonic extraction for 42 ℃temperature, extracting time ask 38 min, the liquid material ratio is 60︰1, it has been verified by testing red meat pitaya pulp pigment absorption value is 0.507. Pigments from Flesh of Red-fleshed Pitaya has strong scavenging effect to DPPH free radicals, with red meat and pitaya pigment concentration in certain concentration-response relationship. As a kind of natural antioxidant has good application prospect, the results show that Pigments from Flesh of Red-fleshed Pitaya have good antioxidant activity.
To study the ultrasonic extraction process of Pigments from Flesh of Red-fleshed Pitaya and antioxidant effect in vitro. The ultrasonic extraction technology of Pigments from Flesh of Red-fleshed Pitaya was optimized by using response surface.On the basis of single factor experiment, pitaya pulp pigment with red flesh absorbance value as a response surface for the optimization of extraction process. With water as extraction solvent, ultrasonic extraction for 42 ℃temperature, extracting time ask 38 min, the liquid material ratio is 60︰1, it has been verified by testing red meat pitaya pulp pigment absorption value is 0.507. Pigments from Flesh of Red-fleshed Pitaya has strong scavenging effect to DPPH free radicals, with red meat and pitaya pigment concentration in certain concentration-response relationship. As a kind of natural antioxidant has good application prospect, the results show that Pigments from Flesh of Red-fleshed Pitaya have good antioxidant activity.
引文
[1]赵志平,杨春霞.火龙果的开发与发展前景[J].中国种业,2006(2):13-14.
[2]缪少霞,王鹏,徐渊金,等.植物源天然食用色素及其开发利用研究进展[J].食用研究与开发,2012,33(7):211-216.
[3]Tenore G C,Novellino E,Basile A.Nutraceutical potential and antioxidant benefits of red pitaya(Hylocereus polyrhizus)extracts[J].Journal of Functional Foods,2012,4(1):129-136.
[4]王永菲,王国成.星点设计法的理论与应用[J].中央民族大学学报,2005,14(3):236-240.
[2]缪少霞,王鹏,徐渊金,等.植物源天然食用色素及其开发利用研究进展[J].食用研究与开发,2012,33(7):211-216.
[3]Tenore G C,Novellino E,Basile A.Nutraceutical potential and antioxidant benefits of red pitaya(Hylocereus polyrhizus)extracts[J].Journal of Functional Foods,2012,4(1):129-136.
[4]王永菲,王国成.星点设计法的理论与应用[J].中央民族大学学报,2005,14(3):236-240.