两份玉米CMS-C恢复系的育性恢复力测定及恢复基因的分子标记定位
|
摘要
为了发掘更多玉米C型不育胞质的强恢复系资源,本研究对2份自交系Z16和7250-14-1进行了恢复能力的测定、恢复基因的遗传分析及恢复基因的分子标记定位。结果表明, Z16和7250-14-1对C黄早四、C478、C698-3和CMo17均表现为育性恢复,而对C48-2则均表现为育性部分恢复。通过对玉米CMS-C不同亚组胞质测交鉴定发现, Z16对G48-2、EC48-2、ES48-2、RB48-2及类48-2均表现为不育性保持,而7250-14-1对G48-2、EC48-2、ES48-2表现为育性部分恢复,对RB48-2和类48-2则表现为不育性保持。Z16和7250-14-1对CMS-T不育系均表现为不育性保持,而对CMS-S不育系则均表现为育性部分恢复。遗传分析显示, Z16对C478和C黄早四的育性恢复均受1对基因控制;而7250-14-1对C黄早四及C478的育性恢复分别受1对基因及2对基因控制。利用(C黄早四×Z16)F2、(C黄早四×7250-14-1)F2群体分别对恢复基因进行分子标记定位,其中Z16的恢复基因被定位于标记B-1至第8染色体短臂末端区域,物理距离为494 kb; 7250-14-1的恢复基因被定位于第8染色体短臂的标记B-1和Chr8-86080之间,物理距离为249kb。该研究不仅为玉米CMS-C"三系"配套的生产利用提供了恢复基因资源,也为玉米CMS-C恢复基因的克隆及恢复机制的研究奠定了一定基础。
The objective of the present study was to identify novel and powerful restorer lines for CMS-C. So maize inbred lines Z16 and 7250-14-1 were crossed with both isonuclear alloplasmic and isoplasmic allonuclear CMS-C, CMS-T, and CMS-S male sterile lines. Self-cross and back-cross were conducted for some of the fertility restored F1 for genetic analysis and restorer gene mapping. Male fertility expression was investigated for all the F1, F2 and backcross populations, showing that Z16 and 7250-14-1 could restore the fertility for C Huangzaosi, C478, C698-3, and CMo17 completely, and partly restore the fertility for C48-2. Z16 could not restore the fertility for G48-2, EC48-2, ES48-2, RB48-2, and Lei48-2, while 7250-14-1 could partly restore the fertility for G48-2, EC48-2, and ES48-2, and maintain the sterility for RB48-2 and Lei48-2. Both Z16 and 7250-14-1 couldn't restore the fertility of CMS-T, and partly restore the fertility for CMS-S. Genetic analysis showed that the fertility restoration was controlled by a pair of dominant genes for Z16 when crossed with C478 or C Huangzaosi. But for 7250-14-1, the fertility restoration was controlled by a pair of dominant genes for C Huangzaosi, and two pairs of complementary dominant genes for C478. Both of the restorer genes for Z16 and 7250-14-1 were mapped on the short arm of chromosome 8 by molecular markers. For Z16, it was mapped within a physical distance of 494 kb from the marker B-1 to the end of the chromosome, and for 7250-14-1, it was located between B-1 and Chr8-86080, with physical distance of 249 kb. This study not only provides some information for the practical application of Z16 and 7250-14-1, but also lays a foundation for the cloning and functional analysis of restorer genes.
The objective of the present study was to identify novel and powerful restorer lines for CMS-C. So maize inbred lines Z16 and 7250-14-1 were crossed with both isonuclear alloplasmic and isoplasmic allonuclear CMS-C, CMS-T, and CMS-S male sterile lines. Self-cross and back-cross were conducted for some of the fertility restored F1 for genetic analysis and restorer gene mapping. Male fertility expression was investigated for all the F1, F2 and backcross populations, showing that Z16 and 7250-14-1 could restore the fertility for C Huangzaosi, C478, C698-3, and CMo17 completely, and partly restore the fertility for C48-2. Z16 could not restore the fertility for G48-2, EC48-2, ES48-2, RB48-2, and Lei48-2, while 7250-14-1 could partly restore the fertility for G48-2, EC48-2, and ES48-2, and maintain the sterility for RB48-2 and Lei48-2. Both Z16 and 7250-14-1 couldn't restore the fertility of CMS-T, and partly restore the fertility for CMS-S. Genetic analysis showed that the fertility restoration was controlled by a pair of dominant genes for Z16 when crossed with C478 or C Huangzaosi. But for 7250-14-1, the fertility restoration was controlled by a pair of dominant genes for C Huangzaosi, and two pairs of complementary dominant genes for C478. Both of the restorer genes for Z16 and 7250-14-1 were mapped on the short arm of chromosome 8 by molecular markers. For Z16, it was mapped within a physical distance of 494 kb from the marker B-1 to the end of the chromosome, and for 7250-14-1, it was located between B-1 and Chr8-86080, with physical distance of 249 kb. This study not only provides some information for the practical application of Z16 and 7250-14-1, but also lays a foundation for the cloning and functional analysis of restorer genes.
引文
[1]Beckett J B.Classification of male-sterile cytoplasms in maize.Crop Sci,1971,11:724-727.
[2]Pring D R,Conde M F,Levings C S I.DNA heterogeneity within the C group of maize male-sterile cytoplasms.Crop Sci,1980,20:159-162.
[3]Duvick D N,Snyder R J,Anderson E G.The chromosomal location of Rf1,a restorer gene for cytoplasmic pollen sterile maize.Genetics,1961,46:1245-1252.
[4]Snyder R J,Duvick D N.Chromosomal location of Rf2,a restorer gene for cytoplasmic male sterile corn.Crop Sci,1969,9:156-157.
[5]Wise R P,Schnable P S.Mapping complementary genes in maize:positioning the rf1 and rf2 nuclear-fertility restorer loci of Texas(T)cytoplasm relative to RFLP and visible markers.Theor Appl Genet,1994,88:785-795.
[6]Cui X Q,Wise R P,Schnable P S.The rf2 nuclear restore gene of male-sterile T-cytoplasm maize.Science,1996,272:1334-1336.
[7]Zhang Z F,Wang Y,Zheng Y L.AFLP and PCR-based markers linked to Rf3,a fertility restorer gene for S cytoplasmic male sterility in maize.Mol Genet Genomics,2006,276:162-169.
[8]李鹏,肖森林,王淑霞,刘娟,赵贤容,陈化榜.玉米S型细胞质雄性不育恢复基因Rf3的精细定位及其候选基因预测.山东农业科学,2014,(8):1-5.Li P,Xiao S L,Wang S X,Liu J,Zhao X R,Chen H B.Fine mapping of fertility restorer gene Rf3 of S-type cytoplasmic male sterility and candidate gene prediction in maize.Shandong Agric Sci,2014,(8):1-5(in Chinese with English abstract).
[9]Feng Y,Zheng Q,Song H,Wang Y,Wang H,Jiang L J,Yan J B,Zheng Y L,Yue B.Multiple loci not only Rf3 involved in the restoration ability of pollen fertility,anther exsertion and pollen shedding to S type cytoplasmic male sterile in maize.Theor Appl Genet,2015,128:2341-2350.
[10]Kheyr-pour A,Gracen V E,Everett H L.Genetics of fertility restoration in the C-group of cytoplasmic male sterility in maize.Genetics,1981,98:379-388.
[11]陈伟程,罗福和,季良越.玉米C型胞质雄花不育的遗传及其在生产上的应用.作物学报,1979,5:21-28.Chen W C,Luo F H,Ji L Y.Some genetic aspects of the C-type cytoplasmic male-sterility in maize and its use in breeding.Acta Agron Sin,1979,5:21-28(in Chinese with English abstract).
[12]Vidakovic M.Genetics of fertility restoration in cytoplasmic male sterility of the C-type(cms-C)in maize.Maydica,1988,33:51-64.
[13]Vidakovic M,Vancetovic J.Complementary genes Rf4,Rf5 and Rf6are not the unique genetic system for fertility restoration in cms-Cof maize(Zea mays L.).Maize Genet Coop Newsl,1997,71:10.
[14]Vidakovic M,Vancetovic J.The existence of a duplicated or parallel genetic system for fertility restoration in cms C(C cytoplasmic male sterility)of maize(Zea mays L.).Maydica,1997,42:313-316.
[15]Sisco P H.Duplications complicate genetic mapping of Rf4,a restorer gene for cms-C cytoplasmic male sterility in corn.Crop Sci,1991,31:1263-1266.
[16]汤继华,刘宗华,陈伟程,胡彦民,季洪强,季良越.玉米C型胞质不育恢复主基因SSR标记.中国农业科学,2001,34:592-596.Tang J H,Liu Z H,Chen W C,Hu Y M,Ji H Q,Ji L Y.The SSRmarkers of the main restorer genes for CMS-C cytoplasmic male sterility in maize.Sci Agric Sin,2001,34:592-596(in Chinese with English abstract).
[17]Kohls S,Stamp P,Knaak C,Messmer R.QTL involved in the partial restoration of male fertility of C-type cytoplasmic male sterility in maize.Theor Appl Genet,2011,123:327-338.
[18]Lee S L J,Earle E D,Gracen V E.The cytology of pollen abortion in s cytoplasmic male-sterile corn anthers.Am J Bot,1980,67:237-245.
[19]Colhoun C W,Steer M W.Microsporogenesis and the mechanism of cytoplasmic male sterility in maize.Ann Bot,1981,48:417-424.
[20]Tatum L A.The southern corn leaf blight epidemic.Science,1971,171:1113-1116.
[21]Weider C,Stamp P,Christov N,Hüsken A,Foueillassar X,Camp K,Munsch M.Stability of cytoplasmic male sterility in maize under different environmental conditions.Crop Sci,2009,49:77-84.
[22]Duvick D N.Cytoplasmic pollen sterility in corn.Adv Genet,1965,13:1-56.
[23]Porebski S,Bailey L G,Baum B R.Modification of a CTAB DNAextraction protocol for plants containing high polysaccharide and polyphenol components.Plant Mol Biol Rep,1997,15:8-15.
[24]Qu J,Liu J.A genome-wide analysis of simple sequence repeats in maize and the development of polymorphism markers from next-generation sequence data.BMC Res Notes,2013,6:1-10.
[25]Kosambi D D.The estimation of map distances from recombination values.Ann Hum Genet,1944,12:172-175.
[26]赵卓凡,黄玲,刘永明,张鹏,魏桂,曹墨菊.玉米CMS-C同质异核不育系育性恢复的遗传研究.遗传,2018,40:402-414.Zhao Z F,Huang L,Liu Y M,Zhang P,Wei G,Cao M J.Genetics of fertility restoration in the isocytoplasm allonuclear C-group of cytoplasmic male sterility in maize.Hereditas(Beijing),2018,40:402-414(in Chinese with English abstract).
[27]Ren R,Nagel B A,Kumpatla S P,Zheng P Z,Cutter G L,Greene T W,Thompson S A.Maize cytoplasmic maize sterility(CMS)C-type restorer Rf4 gene,molecular markers and their use.US2012090047.2012-04-12.
[28]Nan G L,Zhai J,Arikit S,Morrow D,Fernandes J,Mai L,Nguyen N,Meyers B C,Walbot V.MS23,a master basic helix-loop-helix factor,regulates the specification and development of the tapetum in maize.Development,2017,144:163-172.
[29]Komori T,Ohta S,Murai N Y,Kuraya Y,Suzuki S,Hiei Y.Map-based cloning of a fertility restorer gene,Rf-1,in rice(Oryza sativa L.).Plant J,2004,37:315-325.
[30]Wang Z,Zou Y,Li X,Zhang Q,Chen L,Wu H,Su D,Chen Y,Guo J,Luo D,Long Y,Zhong Y,Liu Y G.Cytoplasmic male sterility of rice with boro II cytoplasm is caused by a cytotoxic peptide and is restored by two related PPR motif genes via distinct modes of mRNA silencing.Plant Cell,2006,18:676-687.
[2]Pring D R,Conde M F,Levings C S I.DNA heterogeneity within the C group of maize male-sterile cytoplasms.Crop Sci,1980,20:159-162.
[3]Duvick D N,Snyder R J,Anderson E G.The chromosomal location of Rf1,a restorer gene for cytoplasmic pollen sterile maize.Genetics,1961,46:1245-1252.
[4]Snyder R J,Duvick D N.Chromosomal location of Rf2,a restorer gene for cytoplasmic male sterile corn.Crop Sci,1969,9:156-157.
[5]Wise R P,Schnable P S.Mapping complementary genes in maize:positioning the rf1 and rf2 nuclear-fertility restorer loci of Texas(T)cytoplasm relative to RFLP and visible markers.Theor Appl Genet,1994,88:785-795.
[6]Cui X Q,Wise R P,Schnable P S.The rf2 nuclear restore gene of male-sterile T-cytoplasm maize.Science,1996,272:1334-1336.
[7]Zhang Z F,Wang Y,Zheng Y L.AFLP and PCR-based markers linked to Rf3,a fertility restorer gene for S cytoplasmic male sterility in maize.Mol Genet Genomics,2006,276:162-169.
[8]李鹏,肖森林,王淑霞,刘娟,赵贤容,陈化榜.玉米S型细胞质雄性不育恢复基因Rf3的精细定位及其候选基因预测.山东农业科学,2014,(8):1-5.Li P,Xiao S L,Wang S X,Liu J,Zhao X R,Chen H B.Fine mapping of fertility restorer gene Rf3 of S-type cytoplasmic male sterility and candidate gene prediction in maize.Shandong Agric Sci,2014,(8):1-5(in Chinese with English abstract).
[9]Feng Y,Zheng Q,Song H,Wang Y,Wang H,Jiang L J,Yan J B,Zheng Y L,Yue B.Multiple loci not only Rf3 involved in the restoration ability of pollen fertility,anther exsertion and pollen shedding to S type cytoplasmic male sterile in maize.Theor Appl Genet,2015,128:2341-2350.
[10]Kheyr-pour A,Gracen V E,Everett H L.Genetics of fertility restoration in the C-group of cytoplasmic male sterility in maize.Genetics,1981,98:379-388.
[11]陈伟程,罗福和,季良越.玉米C型胞质雄花不育的遗传及其在生产上的应用.作物学报,1979,5:21-28.Chen W C,Luo F H,Ji L Y.Some genetic aspects of the C-type cytoplasmic male-sterility in maize and its use in breeding.Acta Agron Sin,1979,5:21-28(in Chinese with English abstract).
[12]Vidakovic M.Genetics of fertility restoration in cytoplasmic male sterility of the C-type(cms-C)in maize.Maydica,1988,33:51-64.
[13]Vidakovic M,Vancetovic J.Complementary genes Rf4,Rf5 and Rf6are not the unique genetic system for fertility restoration in cms-Cof maize(Zea mays L.).Maize Genet Coop Newsl,1997,71:10.
[14]Vidakovic M,Vancetovic J.The existence of a duplicated or parallel genetic system for fertility restoration in cms C(C cytoplasmic male sterility)of maize(Zea mays L.).Maydica,1997,42:313-316.
[15]Sisco P H.Duplications complicate genetic mapping of Rf4,a restorer gene for cms-C cytoplasmic male sterility in corn.Crop Sci,1991,31:1263-1266.
[16]汤继华,刘宗华,陈伟程,胡彦民,季洪强,季良越.玉米C型胞质不育恢复主基因SSR标记.中国农业科学,2001,34:592-596.Tang J H,Liu Z H,Chen W C,Hu Y M,Ji H Q,Ji L Y.The SSRmarkers of the main restorer genes for CMS-C cytoplasmic male sterility in maize.Sci Agric Sin,2001,34:592-596(in Chinese with English abstract).
[17]Kohls S,Stamp P,Knaak C,Messmer R.QTL involved in the partial restoration of male fertility of C-type cytoplasmic male sterility in maize.Theor Appl Genet,2011,123:327-338.
[18]Lee S L J,Earle E D,Gracen V E.The cytology of pollen abortion in s cytoplasmic male-sterile corn anthers.Am J Bot,1980,67:237-245.
[19]Colhoun C W,Steer M W.Microsporogenesis and the mechanism of cytoplasmic male sterility in maize.Ann Bot,1981,48:417-424.
[20]Tatum L A.The southern corn leaf blight epidemic.Science,1971,171:1113-1116.
[21]Weider C,Stamp P,Christov N,Hüsken A,Foueillassar X,Camp K,Munsch M.Stability of cytoplasmic male sterility in maize under different environmental conditions.Crop Sci,2009,49:77-84.
[22]Duvick D N.Cytoplasmic pollen sterility in corn.Adv Genet,1965,13:1-56.
[23]Porebski S,Bailey L G,Baum B R.Modification of a CTAB DNAextraction protocol for plants containing high polysaccharide and polyphenol components.Plant Mol Biol Rep,1997,15:8-15.
[24]Qu J,Liu J.A genome-wide analysis of simple sequence repeats in maize and the development of polymorphism markers from next-generation sequence data.BMC Res Notes,2013,6:1-10.
[25]Kosambi D D.The estimation of map distances from recombination values.Ann Hum Genet,1944,12:172-175.
[26]赵卓凡,黄玲,刘永明,张鹏,魏桂,曹墨菊.玉米CMS-C同质异核不育系育性恢复的遗传研究.遗传,2018,40:402-414.Zhao Z F,Huang L,Liu Y M,Zhang P,Wei G,Cao M J.Genetics of fertility restoration in the isocytoplasm allonuclear C-group of cytoplasmic male sterility in maize.Hereditas(Beijing),2018,40:402-414(in Chinese with English abstract).
[27]Ren R,Nagel B A,Kumpatla S P,Zheng P Z,Cutter G L,Greene T W,Thompson S A.Maize cytoplasmic maize sterility(CMS)C-type restorer Rf4 gene,molecular markers and their use.US2012090047.2012-04-12.
[28]Nan G L,Zhai J,Arikit S,Morrow D,Fernandes J,Mai L,Nguyen N,Meyers B C,Walbot V.MS23,a master basic helix-loop-helix factor,regulates the specification and development of the tapetum in maize.Development,2017,144:163-172.
[29]Komori T,Ohta S,Murai N Y,Kuraya Y,Suzuki S,Hiei Y.Map-based cloning of a fertility restorer gene,Rf-1,in rice(Oryza sativa L.).Plant J,2004,37:315-325.
[30]Wang Z,Zou Y,Li X,Zhang Q,Chen L,Wu H,Su D,Chen Y,Guo J,Luo D,Long Y,Zhong Y,Liu Y G.Cytoplasmic male sterility of rice with boro II cytoplasm is caused by a cytotoxic peptide and is restored by two related PPR motif genes via distinct modes of mRNA silencing.Plant Cell,2006,18:676-687.