玉米WOX家族基因的鉴定与分析
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摘要
利用生物信息学的方法鉴定31个玉米的WOX家族基因,对这31个玉米WOX家族基因进行分析。多序列对比结果表明,玉米WOX家族和拟南芥WOX成员均具有高度保守的HOX结构域组helix1-loop-helix2-turnhelix3结构。以拟南芥WOX家族成员作为参照,构建系统进化树,玉米WOX家族成员和拟南芥WOX家族成员共46个基因被分为3个进化支。通过进一步的内含子以及motif分析,结果显示,各个进化支内部的内含子和motif相似度极高,而不同的进化支间有较大的差别。对玉米WOX家族基因的表达模式和功能初步分析,结果表明,有6个WOX家族基因参与了玉米生长发育的调控。
The maize(Zea mays) genome data was utilized to search and identify the three databases(Phytozome, PlantTFDB and NCBI). The 31 families of WOX family genes were identified. Multiple sequence comparisons revealed that the WOX family members of maize had a highly conserved HOX domain. The phylogenetic tree was constructed using the WOX family members of Arabidopsis thaliana as a reference, which was divided into 3 groups.Intron and motif analysis showed that the introns and motifs within each subfamily had extremely high similarities,and there were significant differences among different subfamily. Finally, the expression patterns and functions of the WOX family genes in maize were analyzed.
The maize(Zea mays) genome data was utilized to search and identify the three databases(Phytozome, PlantTFDB and NCBI). The 31 families of WOX family genes were identified. Multiple sequence comparisons revealed that the WOX family members of maize had a highly conserved HOX domain. The phylogenetic tree was constructed using the WOX family members of Arabidopsis thaliana as a reference, which was divided into 3 groups.Intron and motif analysis showed that the introns and motifs within each subfamily had extremely high similarities,and there were significant differences among different subfamily. Finally, the expression patterns and functions of the WOX family genes in maize were analyzed.
引文
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[2]Laux T,Mayer K F,Berger J,et al.The WUSCHEL gene is required for shoot and floral meristem integrity in Arabido psis[J].Development,1996,122(1):87-96.
[3]Haecker A,Gross-Hardt R,Geides B,et al.Expression dynamics of WOX genes mark cell fate decisions during early embryonic patterning in Arabidopsis thaliana[J].Development,2004,131(3):657-668.
[4]Deyhle F,Sarkar A,Tucker E J,et al.WUSCHRL regulates cell differentiation during anther development[J].Developmental Biology,2007,302(1):154-159.
[5]Vandenbussche M,Horstman A,Zethof J,et al.Differential recruitment of WOX transeription factors for lateral derelopment and organ fusion in Petion and Arabidopsis[J].The Plant Cell,2009,21(8):2269-2283.
[6]Deveaux Y,Toffanonioche C,Claisse G,et al.Genes of the most conserved WOX clade in plants affect root and flower development in Arabidopsis[J].BMC Evolutionary Biology,2008,8:291.
[7]Liu J,Sheng L,Xu Y,et al.WOX11 and WOX12 are involved in the first-step cell fate transition during de novo root organcgenesis in Arabidopsis[J].The Plant Cell,2014,26(3):1081-1093.
[8]王俞程,何瑞平,彭献军,等.WOX转录因子家族的研究进展[J].草业科学,2015,32(5):760-769.Wang Y C,He R P,Peng X J,et al.The research progress of WOXtranscription factor family[J].Pratacultural Science,2015,32(5):760-769.(in Chinese)
[9]李晓旭,刘成,李伟,等.番茄WOX转录因子家族的鉴定及其进化、表达分析[J].遗传,2016,38(5):444-460.Li X X,Liu C,Li W.Genome-wide identification,phylogenetic analysis and expression profiling of the WOX family genes in Solanum lycopersicum[J].Hereditas,2016,38(5):444-460.(in Chinese)
[10]于燕杰,张大兵,袁政.WOX蛋白家族调控干细胞发育分子机制的研究进展[J].植物学报,2016,51(4):565-574.Yu Y J,Zhang D B,Yuan Z.The updated functiona lstudy of WOXprotein family in regulating stem cell development[J].Chinese Bulletin of Botany,2016,51(4):565-574.(in Chinese)
[11]Sabatini S,Heidstra R,Wildwater M,et al.SCARECROW is involved in positioning the stem cell niche in the Arabidopsis root meristem[J].Gene Dev,2003,17(3):354-358.
[12]Chen Q,Sun J Q,Zhai Q Z,et al.The basic helix-loop-helix transcription factor MYC2 directly represser PLETHORA expression during jasmonate-mediated modulation of the root ste cell niche in Arabidopsis[J].Plant Cell,2011,23(9):3335-3352.