谷胱甘肽硫转移酶Pi基因多态性与急性脑梗死的关系研究
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摘要
目的探讨谷胱甘肽硫转移酶Pi基因多态性与急性脑梗死的关系。方法选取2011年6月—2014年8月河北省高碑店市医院收治的急性脑梗死患者120例作为急性脑梗死组,另选择同期进行体检的健康者120例作为对照组。采用比色法测定受试者血清谷胱甘肽硫转移酶水平。提取受试者血标本基因组DNA,采用聚合酶链反应(PCR)扩增目的基因并进行基因型分析。结果急性脑梗死组患者血清谷胱甘肽硫转移酶水平为(3.45±1.46)U/ml,低于对照组的(5.14±1.89)U/ml(P<0.05)。两组患者谷胱甘肽硫转移酶Pi基因型分布频率比较,差异有统计学意义(P<0.05);谷胱甘肽硫转移酶Pi(-)基因型者发生急性脑梗死的风险是谷胱甘肽硫转移酶Pi(+)基因型者的1.875倍〔OR=1.875,95%CI(1.271,2.766),P<0.05〕。结论急性脑梗死患者谷胱甘肽硫转移酶活性较低,且谷胱甘肽硫转移酶Pi(-)基因型人群急性脑梗死发生率较高。
Objective To investigate the relation between gene polymorphism of glutathione S-transferase Pi and acute cerebral infarction.Methods From June 2011 to August 2014 in Gaobeidian Hospital,a total of 120 patients with acute cerebral infarction were selected as case group,while 120 healthy cases were selected as control group.Photocolorimetric method was used to detect the serum glutathione S-transferase level;genome DNA was extracted,and PCR was used to analyze the gene types.Results Serum glutathione S-transferase level of case group was(3.45 ± 1.46) U / ml,was statistically significantly lower than that of control group of(5.14 ± 1.89) U / ml(P < 0.05).There was statistically significant difference of gene types of glutathione S-transferase Pi between the two groups(P < 0.05);onset risk of acute cerebral infarction of crowd with negative glutathione S-transferase Pi gene type was 1.875 times of crowd with positive glutathione S-transferase Pi gene type 〔OR = 1.875,95% CI(1.271,2.766),P < 0.05 〕.Conclusion Glutathione S-transferase activity of patients with acute cerebral infarction is relatively low,and crowd with negative glutathione S-transferase Pi gene type has high onset risk of acute cerebral infarction.
Objective To investigate the relation between gene polymorphism of glutathione S-transferase Pi and acute cerebral infarction.Methods From June 2011 to August 2014 in Gaobeidian Hospital,a total of 120 patients with acute cerebral infarction were selected as case group,while 120 healthy cases were selected as control group.Photocolorimetric method was used to detect the serum glutathione S-transferase level;genome DNA was extracted,and PCR was used to analyze the gene types.Results Serum glutathione S-transferase level of case group was(3.45 ± 1.46) U / ml,was statistically significantly lower than that of control group of(5.14 ± 1.89) U / ml(P < 0.05).There was statistically significant difference of gene types of glutathione S-transferase Pi between the two groups(P < 0.05);onset risk of acute cerebral infarction of crowd with negative glutathione S-transferase Pi gene type was 1.875 times of crowd with positive glutathione S-transferase Pi gene type 〔OR = 1.875,95% CI(1.271,2.766),P < 0.05 〕.Conclusion Glutathione S-transferase activity of patients with acute cerebral infarction is relatively low,and crowd with negative glutathione S-transferase Pi gene type has high onset risk of acute cerebral infarction.
引文
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[9]张有金,郭延垒,张艳辉,等.以CDNB为探针测定大鼠肝微粒体中GST活性并优化反应体系[J].华西药学杂志,2013,28(3):259-261.
[10]侯伟,陈保亮.GSTT1、GSTM1、MDR1基因多态性与儿童急性淋巴细胞白血病易感性及化疗反应的关系[J].海南医学院学报,2013,19(11):1493-1496.
[11]Lao X,Peng Q,Lu Y,et al.Glutathione S-transferase gene GSTM1,gene-gene interaction,and gastric cancer susceptibility:evidence from an updated meta-analysis[J].Cancer Cell Int,2014,14(1):127.
[12]李创,丁显平,付莉,等.GSTM1、GSTT1缺失和GSTP1基因多态性与原发无精症的相关性研究[J].中华医学遗传学杂志,2013,30(1):102-105.
[13]吕晓丽,常福厚,尹琴,等.CYP1A1及GSTP1基因多态性与肺癌易感性关系[J].中国公共卫生,2013,29(2):169-172.
[14]姚菲菲,付松波,孙殿军.谷胱甘肽硫转移酶、砷甲基化转移酶基因多态性与地方性砷中毒易感性关系的研究进展[J].中国地方病学杂志,2013,32(1):112-114.
[15]高雅琼.谷胱甘肽硫转移酶基因多态性与疾病关系的研究进展[J].医学综述,2013,19(1):29-31.
[16]White AJ,Teitelbaum SL,Stellman SD,et al.Indoor air pollution exposure from use of indoor stoves and fireplaces in association with breast cancer:a case-control study[J].Environ Health,2014,13(1):108.
[2]周思静,王苒,周俊生,等.GSTM1和GSTT1基因多态性与听力损失相关性的Meta分析[J].中华劳动卫生职业病杂志,2014,32(2):123-125.
[3]Ding H,Liu W,Yu X,et al.Risk association of meningiomas with MTHFR C677T and GSTs polymorphisms:a meta-analysis[J].Int J Clin Exp Med,2014,7(11):3904-3914.
[4]英信江,董频,王国良,等.谷胱甘肽硫转移酶M1基因多态性与喉癌易感性关系的Meta分析[J].中国中西医结合耳鼻咽喉科杂志,2014,4(7):252-257.
[5]程友静,欧阳瑶.慢性阻塞性肺疾病患者静脉血CD40、CD86和GSTO1变化及意义[J].中国老年学杂志,2014,18(7):5066-5067.
[6]张燕丽,屈秋民,郭峰,等.谷胱甘肽硫转移酶Pi基因多态性与Alzheimer病的关系[J].脑与神经疾病杂志,2014,22(5):324-327.
[7]Feng FM,Guo M,Chen Y,et al.Genetic polymorphisms in metabolic enzymes and susceptibility to anti-tuberculosis druginduced hepatic injury[J].Genet Mol Res,2014,13(4):9463-9471.
[8]陈文博,魏晓娟,陈书影,等.中国汉族人群谷胱甘肽硫转移酶M1及T1基因多态性与先天性心脏病发病风险的关系[J].岭南心血管病杂志,2013,19(4):450-456.
[9]张有金,郭延垒,张艳辉,等.以CDNB为探针测定大鼠肝微粒体中GST活性并优化反应体系[J].华西药学杂志,2013,28(3):259-261.
[10]侯伟,陈保亮.GSTT1、GSTM1、MDR1基因多态性与儿童急性淋巴细胞白血病易感性及化疗反应的关系[J].海南医学院学报,2013,19(11):1493-1496.
[11]Lao X,Peng Q,Lu Y,et al.Glutathione S-transferase gene GSTM1,gene-gene interaction,and gastric cancer susceptibility:evidence from an updated meta-analysis[J].Cancer Cell Int,2014,14(1):127.
[12]李创,丁显平,付莉,等.GSTM1、GSTT1缺失和GSTP1基因多态性与原发无精症的相关性研究[J].中华医学遗传学杂志,2013,30(1):102-105.
[13]吕晓丽,常福厚,尹琴,等.CYP1A1及GSTP1基因多态性与肺癌易感性关系[J].中国公共卫生,2013,29(2):169-172.
[14]姚菲菲,付松波,孙殿军.谷胱甘肽硫转移酶、砷甲基化转移酶基因多态性与地方性砷中毒易感性关系的研究进展[J].中国地方病学杂志,2013,32(1):112-114.
[15]高雅琼.谷胱甘肽硫转移酶基因多态性与疾病关系的研究进展[J].医学综述,2013,19(1):29-31.
[16]White AJ,Teitelbaum SL,Stellman SD,et al.Indoor air pollution exposure from use of indoor stoves and fireplaces in association with breast cancer:a case-control study[J].Environ Health,2014,13(1):108.