FNA对短程硝化污泥菌群结构的影响
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摘要
在SBR反应器增加游离亚硝酸(FNA)预处理单元,投加浓度为1.2mg HNO_2-N/L的FNA进行缺氧搅拌4.5h,连续处理3d,考察短程硝化污泥中FNA对氨氧化菌(AOB),丝状菌和微生物菌群结构的影响.研究表明,FNA对AOB有短时抑制作用,并能够抑制优势丝状菌Candidatus_Microthrix(微丝菌属)和Cytophagaceae(噬纤维菌)的增殖,分别由5.1%和1.1%下降到0.78%和几乎不可见.SVI从281mL/g降低到100mL/g左右.NAR能够维持在90%左右,短程硝化不受到破坏.高通量结果显示,FNA处理后微生物菌群结构多样性与丰度出现下降,但Thauera(陶厄氏菌属)和Ottowia出现了增殖,分别增加到5.58%和7.82%,同步硝化反硝化(SND)作用明显,这使得即便只有短程硝化,总氮去除率依然能达到60%以上.
The long-term effects on ammonia-oxidizing bacteria(AOB), filamentous bacteria and microorganism community structures in partial nitrification sludge was investigated by adding a pretreatment unit of free nitrous acid(FNA) in the sequence bath reactor(SBR) for three days at 1.2 mg HNO2-N/L for 4.5. The results showed that FNA had the short-time effect on AOB,Candidatus_Microthrix and Cytophagaceae of the dominant filamentous bacteria were also decreased from 5.1% and 1.1% to 0.78% and almost invisible. Sludge volume index(SVI) maintained at 110 mL/g dropping from 281 mL/g, and the nitrite accumulation rate(NAR) was kept at around 90%, indicating the partial nitrification was not undermined. Furthermore, High-throughput sequencing results showed that the diversity and uniformity of microorganism community decreased. However, the proliferation of Thauera and Ottowia increased to 5.58% and 7.82%. Simultaneous nitrification and denitrification(SND) had significant effects, and the total nitrogen removal rate was nevertheless maintained more than 60% even only with partial nitrification.
The long-term effects on ammonia-oxidizing bacteria(AOB), filamentous bacteria and microorganism community structures in partial nitrification sludge was investigated by adding a pretreatment unit of free nitrous acid(FNA) in the sequence bath reactor(SBR) for three days at 1.2 mg HNO2-N/L for 4.5. The results showed that FNA had the short-time effect on AOB,Candidatus_Microthrix and Cytophagaceae of the dominant filamentous bacteria were also decreased from 5.1% and 1.1% to 0.78% and almost invisible. Sludge volume index(SVI) maintained at 110 mL/g dropping from 281 mL/g, and the nitrite accumulation rate(NAR) was kept at around 90%, indicating the partial nitrification was not undermined. Furthermore, High-throughput sequencing results showed that the diversity and uniformity of microorganism community decreased. However, the proliferation of Thauera and Ottowia increased to 5.58% and 7.82%. Simultaneous nitrification and denitrification(SND) had significant effects, and the total nitrogen removal rate was nevertheless maintained more than 60% even only with partial nitrification.
引文
[1] Gao C D, Fan S X, Jiao E L, et al. Operation and optimization of analternating oxic-anoxic shortcut nitrification-denitrification system[J].Advanced Materials Research, 2014,1030-1032:387-390.
[2]高春娣,王惟肖,李浩,等.SBA法交替缺氧好氧模式下短程硝化效率的优化[J].中国环境科学,2015,35(2):403-409.Gao C D, Wang W X, Li H, et al. Optimization of short-range nitrification efficiency in alternate anoxic aerobic mode by SBR method[J]. China Environmental Science,2015,35(2):403-409.
[3] Peng Y Z, Chen Y,Peng C Y,et al. Nitrite accumulation by aeration controlled in sequencing batch reactors treating domestic wastewater[J]. Water Science and Technology, 2004,50(10):35-43.
[4] Peng Y Z, Zhu G B. Biological nitrogen removal with nitrification and denitrification via nitrite pathway[J]. Appl. Microbiol. Biot., 2006,73(1):15-26.
[5]高春娣,李浩,焦二龙,等.交替好氧缺氧短程硝化及其特性[J].北京工业大学学报,2015,41(1):116-122.Gao C D, Li H, Jiao E L, et al. Alternate oxic-anoxic mode realizing nitritation and its characterization[J]. Journal of Beijing University of Technology, 2015,41(1):116-122.
[6] Yang Q, Peng Y Z, Liu X H, et al. Nitrogen removal via nitrite from municipal wastewater at low temperatures using real-time control to optimize nitrifying communities[J]. Environmental Science Technology,2007,41(23):8159-8164.
[7] Fdzpolanco F, Villaverde S, Garcia P A. Temperature effect on nitifying bacteria activity in biofilters-activation and free ammonia inhibition[J]. Water Science and Technology, 1994,3030(11):121-130.
[8] Ruiz G, Jeison D, Chamy R. Nitrification with high nitrite accumulation for the treatment of wastewater with high ammonia concentration[J]. Water Research, 2003,37(6):1371-1377.
[9] Zhou Y, Oehmen A, Lim M, et al. The role of nitrite and free nitrous acid(FNA)in wastewater treatment plants[J]. Water Research, 2011,45(15):4672-4682.
[10] Guo J H, Peng Y Z, Wang S Y, et al. Effective and robust partial nitrification to nitrite by real-time aeration duration control in an SBR treating domestic wastewate[J]. Process Biochemistry, 2009,44(9):979-985.
[11] Musvoto E V, Lakay M T, Casey T G, et al. Filamentous organism bulking in nutrient removal activated sludge systems.Paper 8:The effect of nitrate and nitrite[J]. Water S A, 1999,25(4):397-407.
[12] Ma Y, Peng Y Z, Wang S Y, et al. Achieving nitrogen removal via nitrite in a pilot-scalecontinuous pre-denitrification plant[J]. Water Research,2009,43(3):563-572.
[13]宋姬晨,王淑莹,杨雄,等.缺/好氧条件下亚硝酸盐的存在对污泥沉降性能的影响[J].中南大学学报(自然科学版),2014,45(4):1361-1368.Song J C, Wang S Y, Yang X, et al. Influence of nitrite on sludge settleability under anoxic and aerobic conditions[J]. Journal of Central South University(Science and Technology), 2014,45(4):1361-1368.
[14]李璐,马娟,宋相蕊.FNA在污水生物脱氮除磷中的抑制效应[J].工业水处理,2014,34(6):5-9.Li L, Ma J, Song X R, et al. Inhibitory effect of free nitrous acid(FNA)on wastewater biological denitrification and dephosphorization[J]. Industrial Water Treatment, 2014,34(6):5-9.
[15]刘甜甜,刘牡,王淑莹,等.盐度耦合FNA对短程反硝化过科程中N2O还原的影响[J].中南大学学报(自然科学版),2013,44(8):35613568.Liu T T, Liu M, Wang S Y, et al. Impact of salinity coupling FNA on N2O reduction during denitrification via nitrite[J]. Journal of Central South University(Science and Technology), 2013,44(8):3561-3568.
[16] Zahedi S,Romero-Guiza M,Icaran P, et al. Optimization of free nitrous acid pre-treatment on waste activated sludge[J]. Bioresource Technology, 2017,252:216-220.
[17] Egli K, Fanger, U, Alvarez, P J J, et al. Enrichment and characterization of an anammox bacterium from a rotating biological contactor treating ammonium-rich leachate[J]. Archives of Microbiology,2001,175(3):198-207.
[18]Yoshida Y, Takahashi K, Saito T et al. The effect of nitrite on aerobic phosphate and denitrifying activity of phosphate-accumulating organisms[J]. Water Science and Technology, 2006,53(6):21-27.
[19] Pijuan M, Ye L, Yuan Z. Free nitrous acid inhibition on the aerobic metabolism of poly-phosphate accumulating organisms[J]. Water Research, 2010,44(20):6063-6072.
[20]马琳娜,刘文龙,张琼,等.游离亚硝酸(FNA)对A2O污泥菌群结构的影响[J].中国环境科学,2017,37(7):2566-2573.Ma L N, Liu W L, Zhang Q, et al. Effect of free nitrous acid(FNA)on microorganism community structures of A2O sludge[J]. China Environmental Science, 2017,37(7):2566-2573.
[21]韩晓宇,张树军,廿一萍,等.以FA与FNA为控制因子的短程硝化启动与维持[J].环境科学,2009,30(3):809-814.Han X Y, Zhang S J, Gan Y P, et al. Start up and maintain of nitritation by the inhibition of FA and FNA[J]. Environmental Science,2009,30(3):809-814.
[22] Strous M, Kuenen J G, Jetten M S M. Key physiology of anaerobic ammonium oxidation[J]. Applied and Environmental Microbiology,1999,65(7):3248-3250.
[23]马斌,委燕,王淑莹,等.基于FNA处理污泥实现城市污水部分短程硝化[J].化工学报,2015,66(12):5054-5059.Ma B, Wei Y, Wang S Y, et al. Achieving partial nitritation in sewage treatment system based on treating activated sludge by FNA[J].CIESC Journal, 2015,66(12):5054-5059.
[24] Anthonisen A C, Loehr R C, Prakasam T, et al. Inhibition of nitrification by ammonia and nirous-acid[J]. Journal Water Pollution Control Federation, 1976,48(5):835-852.[25] Walter W G. Standard methods for the examination of water and wastewater(11th ed.)[J]. American Journal of Public Health&the Nations Health, 1961,51(6):940.[26] Laanbroek H J, Bodelier P L E, Gerards S. Oxygen consumption kinetics of Nitrosomonas europaea and Nitrobacter hamburgensis grown in mixed continuous cultures at different oxygen concentrations[J]. Archives of Microbiology, 1994,161(2):156-162.
[27]高春娣,孙大阳,安冉,等.间歇曝气下短程硝化耦合污泥微膨胀稳定性[J].环境科学,2018,39(7):3271-3278.Gao C D, Sun D Y, An R, et al. Stability of nitritation combining with limited filamentous bulking under intermittent aeration[J].Environmental Science, 2018,39(7):3271-3278.
[28]张宇坤,王淑莹,董,怡君,等.游离氨和游离亚硝酸对亚硝态氮氧化菌活性的影响[J].中国环境科学,2014,34(5):1242-1247.Zhang Y K, Wang S Y, Dong Y J, et al. Effect of FA and FNA on activity of nitrite-oxidising bacteria[J]. China Environmental Science,2014,34(5):1242-1247.
[29] Vadivelu V M, Keller J, Yuan Z G. Effect of free ammonia and free nitrous acid concentration on the anabolic and catabolicprocesses of an enriched Nitrosomonas culture[J]. Biotechnology and bioengineering,2006,95(5):830-839.
[30]周丹丹,马放,董双石,等.溶解氧和有机碳源对同步硝化反硝化的影响[J].环境工程学报,2007,1(4):25-28.Zhou D D, Ma F, Dong S S, et al. Influences of DO and organic carbon on smiultaneous nitrification and denitrification[J]. Chinese Journal of Environmental Engineering, 2007,1(4):25-28.
[31] Navarro R R, Hori T, Inaba T, et al. High-resolution phylogenetic analysis of residual bacterial species of fouled membranes after NaOCl cleaning[J]. Water Research,2016,94:166-175.
[32]高晨晨,游佳,陈轶,等.丝状菌污泥膨胀对脱氮除磷功能菌群的影响[J].环境科学,2018,39(6):2794-2801.Gao C C, You J, Chen Y, et al. Effect of denitrification and phosphorus removal microorganisms in activated sludge bulking caused by filamentous bacteria[J]. Environmental Science, 2018,39(6):2794-2801.
[33] Thomsen T R, Kong Y, Nielsen P H. Ecophysiology of abundant denitrifying bacteria in activated sludge[J]. Ferns Microbiology Ecology, 2010,60(3):370-382.
[34]郑林雪,李军,胡家玮,等.同步硝化反硝化系统中反硝化细菌多样性研究[J].中国环境科学,2015,35(1):116-121.Zheng L X, Li J, Hu J W, et al. Analysis of denitrifying bacteria community composition in simultaneous nitrification and denitrification systems[J]. China Environmental Science, 2015,35(1):116-121.
[35] Spring S, Jackel U M, Kampfer P. Ottowia thiooxydans gen nov sp nov a novel facultatively anaerobic, N2O-producing bacterium isolated from activated sludge, and transfer of Aquaspirillum gracile to Hylemonella gracilis gen nov comb nov[J]. Int. J. Syst. Evol.Microbiol.,2004,54(1):99-106.
[36]蔡丽云,黄泽彬,须子唯,等.处理垃圾渗滤液的SBR中微生物种群与污泥比阻[J].环境科学,2018,39(2):880-888.Cai L Y, Huang Z B, Xu Z W, et al. Microbial communities and sludge specific resistance in two SBRs treating leachate[J]. Environmental Science, 2018,39(2):880-888.
[37]李杨,王芳,杨海滟,等.高通量测序研究李氏禾生态浮床净化污水的微生物群落结构变化[J].西南农业学报,2018,31(9):1903-1911.Li Y, Wang F, Yang H Y, et al. Study on microbial community composition and variation based on high throughput sequencing under leersia hexandra swartz rcological floating bed[J]. Southwest China Journal of Agricultural Sciences, 2018,31(9):1903-1911.
[2]高春娣,王惟肖,李浩,等.SBA法交替缺氧好氧模式下短程硝化效率的优化[J].中国环境科学,2015,35(2):403-409.Gao C D, Wang W X, Li H, et al. Optimization of short-range nitrification efficiency in alternate anoxic aerobic mode by SBR method[J]. China Environmental Science,2015,35(2):403-409.
[3] Peng Y Z, Chen Y,Peng C Y,et al. Nitrite accumulation by aeration controlled in sequencing batch reactors treating domestic wastewater[J]. Water Science and Technology, 2004,50(10):35-43.
[4] Peng Y Z, Zhu G B. Biological nitrogen removal with nitrification and denitrification via nitrite pathway[J]. Appl. Microbiol. Biot., 2006,73(1):15-26.
[5]高春娣,李浩,焦二龙,等.交替好氧缺氧短程硝化及其特性[J].北京工业大学学报,2015,41(1):116-122.Gao C D, Li H, Jiao E L, et al. Alternate oxic-anoxic mode realizing nitritation and its characterization[J]. Journal of Beijing University of Technology, 2015,41(1):116-122.
[6] Yang Q, Peng Y Z, Liu X H, et al. Nitrogen removal via nitrite from municipal wastewater at low temperatures using real-time control to optimize nitrifying communities[J]. Environmental Science Technology,2007,41(23):8159-8164.
[7] Fdzpolanco F, Villaverde S, Garcia P A. Temperature effect on nitifying bacteria activity in biofilters-activation and free ammonia inhibition[J]. Water Science and Technology, 1994,3030(11):121-130.
[8] Ruiz G, Jeison D, Chamy R. Nitrification with high nitrite accumulation for the treatment of wastewater with high ammonia concentration[J]. Water Research, 2003,37(6):1371-1377.
[9] Zhou Y, Oehmen A, Lim M, et al. The role of nitrite and free nitrous acid(FNA)in wastewater treatment plants[J]. Water Research, 2011,45(15):4672-4682.
[10] Guo J H, Peng Y Z, Wang S Y, et al. Effective and robust partial nitrification to nitrite by real-time aeration duration control in an SBR treating domestic wastewate[J]. Process Biochemistry, 2009,44(9):979-985.
[11] Musvoto E V, Lakay M T, Casey T G, et al. Filamentous organism bulking in nutrient removal activated sludge systems.Paper 8:The effect of nitrate and nitrite[J]. Water S A, 1999,25(4):397-407.
[12] Ma Y, Peng Y Z, Wang S Y, et al. Achieving nitrogen removal via nitrite in a pilot-scalecontinuous pre-denitrification plant[J]. Water Research,2009,43(3):563-572.
[13]宋姬晨,王淑莹,杨雄,等.缺/好氧条件下亚硝酸盐的存在对污泥沉降性能的影响[J].中南大学学报(自然科学版),2014,45(4):1361-1368.Song J C, Wang S Y, Yang X, et al. Influence of nitrite on sludge settleability under anoxic and aerobic conditions[J]. Journal of Central South University(Science and Technology), 2014,45(4):1361-1368.
[14]李璐,马娟,宋相蕊.FNA在污水生物脱氮除磷中的抑制效应[J].工业水处理,2014,34(6):5-9.Li L, Ma J, Song X R, et al. Inhibitory effect of free nitrous acid(FNA)on wastewater biological denitrification and dephosphorization[J]. Industrial Water Treatment, 2014,34(6):5-9.
[15]刘甜甜,刘牡,王淑莹,等.盐度耦合FNA对短程反硝化过科程中N2O还原的影响[J].中南大学学报(自然科学版),2013,44(8):35613568.Liu T T, Liu M, Wang S Y, et al. Impact of salinity coupling FNA on N2O reduction during denitrification via nitrite[J]. Journal of Central South University(Science and Technology), 2013,44(8):3561-3568.
[16] Zahedi S,Romero-Guiza M,Icaran P, et al. Optimization of free nitrous acid pre-treatment on waste activated sludge[J]. Bioresource Technology, 2017,252:216-220.
[17] Egli K, Fanger, U, Alvarez, P J J, et al. Enrichment and characterization of an anammox bacterium from a rotating biological contactor treating ammonium-rich leachate[J]. Archives of Microbiology,2001,175(3):198-207.
[18]Yoshida Y, Takahashi K, Saito T et al. The effect of nitrite on aerobic phosphate and denitrifying activity of phosphate-accumulating organisms[J]. Water Science and Technology, 2006,53(6):21-27.
[19] Pijuan M, Ye L, Yuan Z. Free nitrous acid inhibition on the aerobic metabolism of poly-phosphate accumulating organisms[J]. Water Research, 2010,44(20):6063-6072.
[20]马琳娜,刘文龙,张琼,等.游离亚硝酸(FNA)对A2O污泥菌群结构的影响[J].中国环境科学,2017,37(7):2566-2573.Ma L N, Liu W L, Zhang Q, et al. Effect of free nitrous acid(FNA)on microorganism community structures of A2O sludge[J]. China Environmental Science, 2017,37(7):2566-2573.
[21]韩晓宇,张树军,廿一萍,等.以FA与FNA为控制因子的短程硝化启动与维持[J].环境科学,2009,30(3):809-814.Han X Y, Zhang S J, Gan Y P, et al. Start up and maintain of nitritation by the inhibition of FA and FNA[J]. Environmental Science,2009,30(3):809-814.
[22] Strous M, Kuenen J G, Jetten M S M. Key physiology of anaerobic ammonium oxidation[J]. Applied and Environmental Microbiology,1999,65(7):3248-3250.
[23]马斌,委燕,王淑莹,等.基于FNA处理污泥实现城市污水部分短程硝化[J].化工学报,2015,66(12):5054-5059.Ma B, Wei Y, Wang S Y, et al. Achieving partial nitritation in sewage treatment system based on treating activated sludge by FNA[J].CIESC Journal, 2015,66(12):5054-5059.
[24] Anthonisen A C, Loehr R C, Prakasam T, et al. Inhibition of nitrification by ammonia and nirous-acid[J]. Journal Water Pollution Control Federation, 1976,48(5):835-852.[25] Walter W G. Standard methods for the examination of water and wastewater(11th ed.)[J]. American Journal of Public Health&the Nations Health, 1961,51(6):940.[26] Laanbroek H J, Bodelier P L E, Gerards S. Oxygen consumption kinetics of Nitrosomonas europaea and Nitrobacter hamburgensis grown in mixed continuous cultures at different oxygen concentrations[J]. Archives of Microbiology, 1994,161(2):156-162.
[27]高春娣,孙大阳,安冉,等.间歇曝气下短程硝化耦合污泥微膨胀稳定性[J].环境科学,2018,39(7):3271-3278.Gao C D, Sun D Y, An R, et al. Stability of nitritation combining with limited filamentous bulking under intermittent aeration[J].Environmental Science, 2018,39(7):3271-3278.
[28]张宇坤,王淑莹,董,怡君,等.游离氨和游离亚硝酸对亚硝态氮氧化菌活性的影响[J].中国环境科学,2014,34(5):1242-1247.Zhang Y K, Wang S Y, Dong Y J, et al. Effect of FA and FNA on activity of nitrite-oxidising bacteria[J]. China Environmental Science,2014,34(5):1242-1247.
[29] Vadivelu V M, Keller J, Yuan Z G. Effect of free ammonia and free nitrous acid concentration on the anabolic and catabolicprocesses of an enriched Nitrosomonas culture[J]. Biotechnology and bioengineering,2006,95(5):830-839.
[30]周丹丹,马放,董双石,等.溶解氧和有机碳源对同步硝化反硝化的影响[J].环境工程学报,2007,1(4):25-28.Zhou D D, Ma F, Dong S S, et al. Influences of DO and organic carbon on smiultaneous nitrification and denitrification[J]. Chinese Journal of Environmental Engineering, 2007,1(4):25-28.
[31] Navarro R R, Hori T, Inaba T, et al. High-resolution phylogenetic analysis of residual bacterial species of fouled membranes after NaOCl cleaning[J]. Water Research,2016,94:166-175.
[32]高晨晨,游佳,陈轶,等.丝状菌污泥膨胀对脱氮除磷功能菌群的影响[J].环境科学,2018,39(6):2794-2801.Gao C C, You J, Chen Y, et al. Effect of denitrification and phosphorus removal microorganisms in activated sludge bulking caused by filamentous bacteria[J]. Environmental Science, 2018,39(6):2794-2801.
[33] Thomsen T R, Kong Y, Nielsen P H. Ecophysiology of abundant denitrifying bacteria in activated sludge[J]. Ferns Microbiology Ecology, 2010,60(3):370-382.
[34]郑林雪,李军,胡家玮,等.同步硝化反硝化系统中反硝化细菌多样性研究[J].中国环境科学,2015,35(1):116-121.Zheng L X, Li J, Hu J W, et al. Analysis of denitrifying bacteria community composition in simultaneous nitrification and denitrification systems[J]. China Environmental Science, 2015,35(1):116-121.
[35] Spring S, Jackel U M, Kampfer P. Ottowia thiooxydans gen nov sp nov a novel facultatively anaerobic, N2O-producing bacterium isolated from activated sludge, and transfer of Aquaspirillum gracile to Hylemonella gracilis gen nov comb nov[J]. Int. J. Syst. Evol.Microbiol.,2004,54(1):99-106.
[36]蔡丽云,黄泽彬,须子唯,等.处理垃圾渗滤液的SBR中微生物种群与污泥比阻[J].环境科学,2018,39(2):880-888.Cai L Y, Huang Z B, Xu Z W, et al. Microbial communities and sludge specific resistance in two SBRs treating leachate[J]. Environmental Science, 2018,39(2):880-888.
[37]李杨,王芳,杨海滟,等.高通量测序研究李氏禾生态浮床净化污水的微生物群落结构变化[J].西南农业学报,2018,31(9):1903-1911.Li Y, Wang F, Yang H Y, et al. Study on microbial community composition and variation based on high throughput sequencing under leersia hexandra swartz rcological floating bed[J]. Southwest China Journal of Agricultural Sciences, 2018,31(9):1903-1911.