摘要
为建立一种高效准确的烟草黑胫病抗性鉴定方法,以4种具有不同抗性特性的烟草品种为材料,研究培养基附加不同浓度黑胫病病菌毒素及不同胁迫时间对烟草种子萌发和根系生长的影响。结果表明,烟草黑胫病病菌毒素胁迫对烟草种子的发芽率和根系生长均有抑制作用,抑制效果随胁迫浓度的增加而增强,且不同品种对毒素胁迫的耐受力不同,利用体积浓度为50%的毒素胁迫15 d时,抗病品种G28、中抗品种YN116、中感品种净叶黄、感病品种红花大金元的根系长进培养基中的株数比率分别为79.38%、70.74%、33.55%和13.36%。初步确定鉴别烟草种子抗性特性的最佳粗毒素胁迫浓度为50%(即含蛋白质13.53μg/m L,含糖19.64 mg/m L)、最佳鉴定时间为胁迫15 d,利用该方法可在短期内高效准确地鉴定出不同品种(种质)对烟草黑胫病的抗感水平,加快烟草抗黑胫病育种进程。
In order to establish an efficient and accurate method for identification of resistance to tobacco black shank, experiments were performed using 4 tobacco varieties with different resistance characteristics. The toxin was added to the medium to study the effects of different concentrations of toxin stress and different stress time on tobacco seed germination and root growth. The results showed that the toxin stress of black shank disease could inhibit the germination and root growth of tobacco seedlings, and the inhibitory effect increased with the increase of toxin concentration. Different varieties have different tolerance to toxin stress. When the toxin was used at a concentration of 50% for 15 days, the ratio of the number of plants in the root growth medium of G28, YN116,Jingyehuang and Hongda was 79.38%, 70.74%, 33.55% and 13.36%, respectively. It was preliminarily determined that the optimal crude toxin concentration for identifying the resistance level of different varieties(germplasm) to tobacco black shank was 50%(protein content 13.53 μg/mL, sugar content 19.64 mg/mL), and the optimal stress time was 15 days. This method can effectively and accurately identify the resistance level of different varieties(germplasm) to tobacco black shank in a short period of time, and accelerate the breeding process of tobacco resistance to black shank.
引文
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