植物研究中的流式细胞术及其在烟草中的应用进展
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摘要
流式细胞术作为一种快速、灵敏的细胞分析分选技术,目前已广泛应用于植物学研究的多个领域。为了拓展流式细胞术在烟草研究中的应用范围,介绍了流式细胞仪的工作原理,并阐述了流式细胞术在植物细胞核DNA含量和倍性分析、植物核型分析及分选、细胞分选与其他技术的联合等学科领域的应用概况。在总结流式细胞术在烟草相关研究领域应用现状的基础上,展望了流式细胞术在烟草单细胞组学、病害防治和烟气毒理学检测等方面进一步研究与应用的可能性。
Flow cytometry is a rapid and sensitive technology in cell analysis and sorting. At present, it has been widely used in many fields of plant research. In order to explore the application of flow cytometry in tobacco research, this article described the working principle of flow cytometry, and reviewed the current application of flow cytometry in plant research, such as DNA content in plant nuclear and ploidy analysis,chromosomal karyotype analysis and sorting, cell sorting and integrating with other technologies. Furthermore,the applications of flow cytometry in tobacco research were reviewed, and the possibility of further application in tobacco single-cell omics, disease control and cigarette smoke toxicology detection was prospected.
Flow cytometry is a rapid and sensitive technology in cell analysis and sorting. At present, it has been widely used in many fields of plant research. In order to explore the application of flow cytometry in tobacco research, this article described the working principle of flow cytometry, and reviewed the current application of flow cytometry in plant research, such as DNA content in plant nuclear and ploidy analysis,chromosomal karyotype analysis and sorting, cell sorting and integrating with other technologies. Furthermore,the applications of flow cytometry in tobacco research were reviewed, and the possibility of further application in tobacco single-cell omics, disease control and cigarette smoke toxicology detection was prospected.
引文
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[2]姜倩倩,曹慧.流式细胞术在高等植物细胞学研究中的应用[J].潍坊学院学报,2011,11(6):82-85.JIANG Qianqian,CAO Hui.Applications of flow cytometry in the study of higher plant cytology[J].Journal of Weifang University,2011,11(6):82-85.
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[5]Zeng Z H,Huang H H,Han N,et al.Endopolyploidy levels in barley vary in different root types and significantly decrease under phosphorus deficiency[J].Plant Physiology and Biochemistry,2017,118:11-21.
[6]Cápal P,Vrána J,KubalákováM,et al.Chromosomal allocation of DNA sequences in wheat using flow-sorted chromosomes[J].Chromosome and Genomic Engineering in Plants,2016,1469:157-170.
[7]张芳,光应芝,张前前,等.两种北极微型浮游植物的荧光差异性分析[J].海洋与沼泽,2013,44(5):1189-1193.ZHANG Fang,GUANG Yingzhi,ZHANG Qianqian,et al.Comparison of in vivo fluorescence properties between two arctic microalgal species[J].Oceanologia et Limnologia Sinica,2013,44(5):1189-1193.
[8]Baniaga A E,Arrigo N,Barker M S.The small nuclear genomes of Selaginella are associated with a low rate of genome size evolution[J].Genome Biology and Evolution,2016,8(5):1516-1525.
[9]王雨,林茂,林更铭,等.流式影像术在海洋浮游植物分类研究中的应用[J].海洋科学进展,2010,28(2):266-274.WANG Yu,LIN Mao,LIN Gengming,et al.Application of flow cytometry and microscopy(FlowCAM)in marine phytoplankton taxonomic studies[J].Advances in Marine Science,2010,28(2):266-274.
[10]Carter A D,Bonyadi R,Gifford M L.The use of fluorescence-activated cell sorting in studying plant development and environmental responses[J].The International Journal of Developmental Biology,2013,57(6/7/8):545-552.
[11]Julius M H,Masuda T,Herzenberg L A.Demonstration that antigen-binding cells are precursors of antibody-producing cells after purification with a fluorescence-activated cell sorter[J].Proceedings of the National Academy of Sciences of the United States of America,1972,69(7):1934-1938.
[12]Borges F,Gomes G,Gardner R,et al.Comparative transcriptomics of Arabidopsis sperm cells[J].Plant Physiology,2008,148(2):1168-1181.
[13]Bargmann B O,Birnbaum K D.Fluorescence activated cell sorting of plant protoplasts[J].Journal of Visualized Experiments,2010(36):1673.
[14]Lo Y S,Cheng N,Hsiao L J,et al.Actin in mung bean mitochondria and implications for its function[J].The Plant Cell,2011,23(10):3727-3744.
[15]Birnbaum K,Shasha D E,Wang J Y,et al.A gene expression map of the Arabidopsis root[J].Science,2003,302(5652):1956-1960.
[16]Dole?el J,?íhalíkováJ,Lucretti S.A high-yield procedure for isolation of metaphase chromosomes from root tips of Vicia faba L.[J].Planta,1992,188(1):93-98.
[17]Yan H H,Martin S L,Bekele W A,et al.Genome size variation in the genus Avena[J].Genome,2016,59(3):209-220.
[18]周翔宇,林峰,徐莉,等.基于流式细胞术的5种鼠尾草基因组C值测定[J].南方农业学报,2017,48(6):960-965.ZHOU Xiangyu,LIN Feng,XU Li,et al.Measurement of genomic C value of five Salvia species by flow cytometry[J].Journal of Southern Agriculture,2017,48(6):960-965.
[19]Pirrello J,Deluche C,Frangne N,et al.Transcriptome profiling of sorted endoreduplicated nuclei from tomato fruits:how the global shift in expression ascribed to DNA ploidy influences RNA-Seq data normalization and interpretation[J].The Plant Journal,2018,93(2):387-398.
[20]Ma Y P,W J X,Yu Z Y,et al.Characterization of ploidy levels in Chrysanthemum L.by flow cytometry[J].Journal of Forestry Research,2014,26(3):771-775.
[21]Bourdon M,Coriton O,Pirrello J,et al.In planta quantification of endoreduplication using fluorescent in situ hybridization(FISH)[J].The Plant Journal:for Cell and Molecular Biology,2011,66(6):1089-1099.
[22]Chansler M T,Ferguson C J,Fehlberg S D,et al.The role of polyploidy in shaping morphological diversity in natural populations of Phlox amabilis[J].American Journal of Botany,2016,103(9):1546-1558.
[23]Alatar A A,Faisal M,Abdel-Salam E M,et al.Efficient and reproducible in vitro regeneration of Solanum lycopersicum and assessment genetic uniformity using flow cytometry and SPAR methods[J].Saudi Journal of Biological Sciences,2017,24(6):1430-1436.
[24]Guo L Q,Xu W T,Zhang Y,et al.Inducing triploids and tetraploids with high temperatures in Populus sect.Tacamahaca[J].Plant Cell Reports,2017,36(2):313-326.
[25]Gray J W,Carrano A V,Steinmetz L L,et al.Chromosome measurement and sorting by flow systems[J].Proceedings of the National Academy of Sciences of the United States of America,1975,72(4):1231-1234.
[26]Arumuganathan K,Martin G B,Telenius H,et al.Chromosome 2-specific DNA clones from flow-sorted chromosomes of tomato[J].Molecular and General Genetics MGG,1994,242(5):551-558.
[27]Macas J,Gualberti G,NouzováM,et al.Construction of chromosome-specific DNA libraries covering the whole genome of field bean(Vicia faba L.)[J].Chromosome Research,1996,4(7):531-539.
[28]Macas J,Dole?el J,Lucretti S,et al.Localization of seed protein genes on flow-sorted field bean chromosomes[J].Chromosome Research,1993,1(2):107-115.
[29]Galbraith D W,Sliwinska E,Samadder P.Nuclear cytometry:analysis of the patterns of DNA synthesis and transcription using flow cytometry,confocal microscopy,and RNA sequencing[J].Methods in Molecular Biology,2018,1678:371-392.
[30]Chamrád I,U?inovskáJ,PetrovskáB,et al.Identification of plant nuclear proteins based on a combination of flow sorting,SDS-PAGE,and LC-MS/MS analysis[J].Methods in Molecular Biology,2018,1696:57-79.
[31]Zhang C Q,Barthelson R A,Lambert G M,et al.Global characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei[J].Plant Physiology,2008,147(1):30-40.
[32]Birnbaum K,Jung J W,Wang J Y,et al.Cell type-specific expression profiling in plants via cell sorting of protoplasts from fluorescent reporter lines[J].Nature Methods,2005,2(8):615-619.
[33]Petersson S V,Lindén P,Moritz T,et al.Cell-type specific metabolic profiling of Arabidopsis thaliana protoplasts as a tool for plant systems biology[J].Metabolomics,2015,11(6):1679-1689.
[34]Carqueijeiro I,Guim?raes A L,Bettencourt S,et al.Isolation of cells specialized in anticancer alkaloid metabolism by fluorescence-activated cell sorting[J].Plant Physiology,2016,171(4):2371-2378.
[35]von Behrens I,Komatsu M,Zhang Y X,et al.Rootless with undetectable meristem 1 encodes a monocot-specific AUX/IAA protein that controls embryonic seminal and post-embryonic lateral root initiation in maize[J].The Plant Journal,2011,66(2):341-353.
[36]Li M,Berendzen K W,Sch?ffl F.Promoter specificity and interactions between early and late Arabidopsis heat shock factors[J].Plant Molecular Biology,2010,73(4/5):559-567.
[37]Zhang X D,Feng J J,Wang H,et al.Bivariate flow cytometric analysis and sorting of different types of maize starch grains[J].Cytometry.Part A:the Journal of the International Society for Analytical Cytology,2018,93(2):213-221.
[38]Asara Y,Marchal J A,Carrasco E,et al.Cadmium modifies the cell cycle and apoptotic profiles of human breast cancer cells treated with 5-fluorouracil[J].International Journal of Molecular Sciences,2013,14(8):16600-16616.
[39]Moerloose K B,Robays L J,Maes T,et al.Cigarette smoke exposure facilitates allergic sensitization in mice[J].Respiratory Research,2006,7:49.
[40]Chen K,Pociask D A,McAleer J P,et al.IL-17RA is required for CCL2 expression,macrophage recruitment,and emphysema in response to cigarette smoke[J].PLoS One,2011,6(5):e20333.
[41]Gál K,Csehá,Szalay B,et al.Effect of cigarette smoke and dexamethasone on Hsp72 system of alveolar epithelial cells[J].Cell Stress and Chaperones,2011,16(4):369-378.
[42]Hao J W,Shi F D,Abdelwahab M,et al.Nicotinic receptorβ2 determines NK cell-dependent metastasis in a murine model of metastatic lung cancer[J].PLoSOne,2013,8(2):e57495.
[43]CronjéM J,Snyman M,Bornman L,et al.A rapid and reliable flow cytometric method for determining Hsp70levels in tobacco protoplasts[J].Methods in Cell Science,2003,25(3/4):237-246.
[44]Takahashi S,Kojo K H,Kutsuna N,et al.Differential responses to high-and low-dose ultraviolet-B stress in tobacco Bright Yellow-2 cells[J].Frontiers in Plant Science,2015,6:254.
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