液相色谱-荧光检测法快速检测饲料中黄曲霉毒素B_1
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摘要
饲料样品经过乙腈-水溶液(乙腈∶水=84∶16)提取,与三氟乙酸衍生后经高效液相色谱-荧光检测法测定。结果表明,饲料样品中黄曲霉毒素B_1回收率大于75%,样品检出限和定量限分别为2.0μg/kg(S/N=3)和6.7μg/kg(S/N=10)。
In this experiment,the feed samples were extracted with acetonitrile aqueous solution(acetonitrile∶water=84∶16),and determined by high performance liquid chromatography fluorescence detection after derivation by trifluoroacetic acid.The results showed that the recovery rate of aflatoxinB1 in the feed samples was greater than 75%,and the detection limit and quantitative limit of the samples were 2.0 g/kg(S/N=3) and 6.7 g/kg(S/N=10),respectively.
In this experiment,the feed samples were extracted with acetonitrile aqueous solution(acetonitrile∶water=84∶16),and determined by high performance liquid chromatography fluorescence detection after derivation by trifluoroacetic acid.The results showed that the recovery rate of aflatoxinB1 in the feed samples was greater than 75%,and the detection limit and quantitative limit of the samples were 2.0 g/kg(S/N=3) and 6.7 g/kg(S/N=10),respectively.
引文
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[2] GIRAY B,GIRGIN G,ENGIN A B.Aflatoxin levels in wheat samples consumed in some regions of turkey [J].Food Control,2007,18:23-29.
[3] BRAICU C,PUIA C,BODOKLI E.Screening and quantification of aflatoxins and ochratoxin A in different cereals cultivated in Romania using thin-layer chromatography densitometry[J].J Food Qual,2008,31(1):108-120.
[4] LI P W,ZHANG Q,ZHANG W.Development of a class-specific monoclonal antibody based ELISA for aflatoxins in peanut[J].Food Chem,2009,115:313-317.
[5] KHAYOON W S,SAAD B,YAN C B,et al.Determination of aflatoxins in animal feeds by HPLC with multifunctional column clean-up[J].Food Chemistry,2009,118(2010)882-886.
[6] PENA R,ALCARAZ M,ARCE L,et al.Screening of aflatoxins in feed samples using a flow system coupled to capillary electrophoresis[J].J Chromatogr A,2002,967(2):303-314.
[7] 莫彩娜,杨曦,黄智成.高效液相色谱-串联质谱法测定水产品中黄曲霉毒素G2、G1、B2、B1[J].广州化工,2011,39(20):92-94.