几种水产品中雌激素的检测及其微生物降解的研究
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摘要
部分养殖户在池塘中人为添加具有"催生"能力的激素饲料,以达到促进水产品快速生长的目的。过量的雌激素已经成为影响水产品安全和人类健康的一个重大问题。生物降解是一种环境友好且经济安全的方法,可以用来减少雌激素对环境的危害。建立了一种从水产品中高效提取雌二醇的方法,并利用高效液相色谱(HPLC)法对市售花蛤、蛏子、海螺、海锥、黄花鱼、青虾等水产品中的雌二醇进行检测。结果表明:在海螺和海锥中发现了雌二醇的富集。选择4株天然降解甾体类化合物的菌株开展降解雌二醇的研究,结果表明:这4种菌株均具有降解雌二醇的能力,其中睾丸酮丛毛单胞菌(CT1)的降解效率最高。
Estrogen with biological activities has been added into the feed in order to improve growth of aquatic products. Excess estrogen has become a major problem, which was effect on the safety of aquatic products and human health. Biodegradation is an environment-friendly, economical way to reduce environmental disruption by estrogen. We have established an efficient method for extraction and determination of estradiol from aquatic products by high performance liquid chromatography(HPLC),such as Meretrix meretrix L, Sinonovacula constricta L, Busycon canaliculatu, Sea cone, Larimichthys,Macrobrachium nipponense. The results showed that the enrichments of estradiol were found in Busycon canaliculatu and Sea cones. We selected four bacterial strains isolated from the environment, which could degrade steroid compounds. The results showed that all four strains could degrade estradiol, and the degradation efficiency of Comamonas testosteroni(CT1) was the highest.
Estrogen with biological activities has been added into the feed in order to improve growth of aquatic products. Excess estrogen has become a major problem, which was effect on the safety of aquatic products and human health. Biodegradation is an environment-friendly, economical way to reduce environmental disruption by estrogen. We have established an efficient method for extraction and determination of estradiol from aquatic products by high performance liquid chromatography(HPLC),such as Meretrix meretrix L, Sinonovacula constricta L, Busycon canaliculatu, Sea cone, Larimichthys,Macrobrachium nipponense. The results showed that the enrichments of estradiol were found in Busycon canaliculatu and Sea cones. We selected four bacterial strains isolated from the environment, which could degrade steroid compounds. The results showed that all four strains could degrade estradiol, and the degradation efficiency of Comamonas testosteroni(CT1) was the highest.
引文
[1]李本纲,崔司宇.中国天然雌激素排放清单和风险评价[J].城市环境与城市生态,2011,24(4):24-28.
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[11]孙岩,刘慧慧,徐英江,等.不同海洋介质中环境激素类物质的分析测定和生态风险评价研究进展[J].中国渔业质量与标准,2015,5(1):11-18.
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[15]Xiong G,Draus E,Luo Y,et al.3alpha-Hydroxysteroid dehydrogenase/carbonyl reductase as a tool for isolation and characterization of a new marine steroid degrading bacterial strain[J].Chemico-Biological Interactions,2009,178:206-210.
[16]Zhang T,Xiong G,Maser E.Characterization of the steroid degrading bacterium S19-1 from the Baltic Seaat Kiel,Germany[J].Chemico-Biological Interactions,2011,191:83-88.
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[19]Chen J,Ai Y,Zhou Y,et al.Characterization and heterologous expression of testosterone-inducible regulator from Comamonas testosteroni in Escherichia coli[J].Chinese Journal of Agricultural Biotechnology,2009,(6):41-47.
[20]Shaba S K,Sasson G,Doron-Faigenboim A,et al.Specific microbiome-dependent mechanisms underlie the energy harvest efficiency of ruminants[J].International Society for Microbial Ecology,2016,(10):2958-2972.
[2]陈蕾,王郑,曹世玮.畜禽养殖排放的雌激素对周边水体环境的影响[J].生态环境学报,2014,23(2):359-364.
[3]罗慧玉,徐鹏,闫伟伟,等.改进MSPD-HPLC法快速检测猪血豆腐中镇静剂及雌激素残留[J].食品研究与开发,2017,38(4):147-150.
[4]Sang Y Y,Xiong G M,Maser E.Identification of a new steroid degrading bacterial strain H5 from the Baltic Sea and isolation of two estradiol inducible genes[J].The Journal of Steroid Biochemistry and Molecular Biology,2012,129:22-30.
[5]罗平.高效液相色谱法测定地表水中5种雌激素残留[J].化学分析计量,2017,26(5):78-81.
[6]黄何何.快速溶剂萃取-气相色谱/质谱联用法测定儿童食品接触用品中28种环境雌激素[J].福建分析测试,2018,27(4):23-32.
[7]卢巧梅.水产品中多种雌激素残留的质谱分析方法研究[J].分析测试技术与仪器,2016,22(1):1-8.
[8]冯月超,贾丽,王建凤,等.超高效液相色谱-质谱联用法检测鱼肉中23种性激素残留量[J].食品安全质量检测学报,2018,16(9):4417-4426.
[9]房克艳,赵超敏,邓晓军,等.固相萃取-超高效液相色谱-三重四极杆质谱法同时测定饲料中5种雌激素[J].现代食品科技,2018,34(11):1-8.
[10]季晓亚,李娜,袁圣武,等.环境雌激素生物效应的作用机制研究进展[J].生态毒理学报,2017,12(1):38-51.
[11]孙岩,刘慧慧,徐英江,等.不同海洋介质中环境激素类物质的分析测定和生态风险评价研究进展[J].中国渔业质量与标准,2015,5(1):11-18.
[12]Maser E,Xiong G.The Comamonas testosteroni steroid biosensor system(COSS)-reflection on other methods[J].The Journal of Steroid Biochemistry and Molecular Biology,2010,121:633-640.
[13]Talalay P,Dobson M M,Tapley D F.Oxidative degradation of testosterone by adaptive enzymes[J].Nature,1952,170:620-621.
[14]Schleheck D,Knepper T P,Fischer K,et al.Mineralization of individual congeners of linear alkylbenzenesulfonate by defined pairs of Heterotrophic bacteria[J].Applied and Environmental Microbiology,2004,70:4053-4063.
[15]Xiong G,Draus E,Luo Y,et al.3alpha-Hydroxysteroid dehydrogenase/carbonyl reductase as a tool for isolation and characterization of a new marine steroid degrading bacterial strain[J].Chemico-Biological Interactions,2009,178:206-210.
[16]Zhang T,Xiong G,Maser E.Characterization of the steroid degrading bacterium S19-1 from the Baltic Seaat Kiel,Germany[J].Chemico-Biological Interactions,2011,191:83-88.
[17]Liang L,Song X,Kong J,et al.Anaerobic biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons by a facultative anaerobe Pseudomonas sp.JP1[J].Biodegradation,2014,25:825-833.
[18]Liao X,Chen T,Xie H,et al.Soil as contamination and its risk assessment in areas near the industrial districts of Chenzhou City,Southern China[J].Environment International,2005,31:791-798.
[19]Chen J,Ai Y,Zhou Y,et al.Characterization and heterologous expression of testosterone-inducible regulator from Comamonas testosteroni in Escherichia coli[J].Chinese Journal of Agricultural Biotechnology,2009,(6):41-47.
[20]Shaba S K,Sasson G,Doron-Faigenboim A,et al.Specific microbiome-dependent mechanisms underlie the energy harvest efficiency of ruminants[J].International Society for Microbial Ecology,2016,(10):2958-2972.