饮用水遗传毒性测试中TA98和TA100的敏感度和可靠度比较
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摘要
为了寻找适合我国水质特征的Ames试验简化方法,作者通过广泛的文献调研,对近30年以来,我国研究者用Ames试验评价饮用水和水源水的遗传毒性的实验数据进行了总结和分析。结果发现,TA98-S9测试体系的敏感度和可靠度最高,TA98+S9次之,TA100-S9与TA100+S9 2个测试体系对Ames试验结果的影响几乎可以忽略。只用1个TA98-S9测试体系的方法 1,仅用25%的工作量,可获得91%的致突变阳性检出率和74%的有效数据率;采用TA98-S9和TA98+S9 2个测试体系的方法 2,使用50%的工作量,可获得96%的阳性检出率和96%的有效数据率。据此,作者建议:采用Ames试验评价我国饮用水和水源水的遗传毒性时,可以将通常采用的4个测试体系的方法 4,简化为只采用TA98-S9与TA98+S9 2个测试体系的方法 2。在仅进行致突变性定性评价时,或者在费用或水样量不足等条件下,则可使用更为简化的方法 1,更利于Ames试验的广泛应用。
In order to identify a simplified Ames test method optimized for the characteristics and quality of water in China, Ames test data of drinking water and source water samples from different regions in China obtained from papers published in the last 30 years was summarized and analyzed. It was found that TA98-S9 is the most sensitive and reliable testing system, TA98 +S9 comes second. On the other hand, TA100-S9 and TA100 +S9 systems have negligible impacts on Ames test results. Method 1, which uses the TA98-S9 system, can achieve a 91% positive detection rate and a 74% reliability rate with 25% required work and cost compared to traditional method.Method 2, which uses the TA98-S9 and TA98+S9 systems can achieve 96% of positive detection rate and 96% of reliability rate with 50% required work and cost compared to traditional method. The above findings suggest thatAmes test can be simplified from the traditional 4 systems into two systems using TA98-S9 and TA98+S9 for the evaluation of drinking water or source water genotoxicity in China. In case of limited budgets, low sample volume,or when only a qualitative analysis required, the method can be simplified to using only the TA98-S9 system,which may extend the usage of Ames test.
In order to identify a simplified Ames test method optimized for the characteristics and quality of water in China, Ames test data of drinking water and source water samples from different regions in China obtained from papers published in the last 30 years was summarized and analyzed. It was found that TA98-S9 is the most sensitive and reliable testing system, TA98 +S9 comes second. On the other hand, TA100-S9 and TA100 +S9 systems have negligible impacts on Ames test results. Method 1, which uses the TA98-S9 system, can achieve a 91% positive detection rate and a 74% reliability rate with 25% required work and cost compared to traditional method.Method 2, which uses the TA98-S9 and TA98+S9 systems can achieve 96% of positive detection rate and 96% of reliability rate with 50% required work and cost compared to traditional method. The above findings suggest thatAmes test can be simplified from the traditional 4 systems into two systems using TA98-S9 and TA98+S9 for the evaluation of drinking water or source water genotoxicity in China. In case of limited budgets, low sample volume,or when only a qualitative analysis required, the method can be simplified to using only the TA98-S9 system,which may extend the usage of Ames test.
引文
[1]朱振岗.某市恶性肿瘤死亡率增高与饮用水水质污染关系的研究[J].环境与健康杂志,1984,1(3):3-4Zhu Z G.Study on the relationship between increasing mortality rate of malignant tumor and pollution of drinking water in a city[J].Journal of Environment and Health,1984,1(3):3-4(in Chinese)
[2]许宁,杨璐璐,董梦萌,等.4种消化道肿瘤与饮用水关系的生存分析[J].环境与健康杂志,2015,32(3):231-234Xu N,Yang L L,Dong M M,et al.Survival analysis of four gastrointestinal tumors and drinking shallow groundwater in a basin in middle China[J].Journal of Environment and Health,2015,32(3):231-234(in Chinese)
[3]Fenster L,Waller K,Windhmn G,et a1.Trihalomethane levels in home tap water and semen quality[J].Epidemiology,2003,14:650-658
[4]朱惠刚.水中有机致突变物综合评价指标探讨[J].上海环境科学,1995,14(10):44-47Zhu H G.Comprehensive evaluation index of organic mutagens in water[J].Shanghai Environmental Sciences,1995,14(10):44-47(in Chinese)
[5]张建超.检测水体遗传毒性的短期遗传毒性试验方法[J].科技信息,2013,11:88-89Zhang J C.Widely used short-term genotoxicity assays in water detection[J].Science and Technology Information,2013,11:88-89(in Chinese)
[6]王占生,刘文君,张锡辉.微污染水源饮用水处理[M].北京:中国建筑工业出版社,2016:32-34,280-282,295-297Wang Z S,Liu W J,Zhang X H.Treatment of Micro-polluted Source Water for Drinking[M].Beijing:China Architecture&Building Press,2016:32-34,280-282,295-297(in Chinese)
[7]刘世良,张惠娟,何尚浦,等.自来水有机提取物致突变试验及强致突变物的分离与鉴定[J].湖北预防医学杂志,1994,5(3):12-18Liu S L,Zhang H J,He S P,et al.Mutagenicity test of organic extracts from tap water and isolation and identification of strong mutagens[J].Hubei Journal of Preventive Medicine,1994,5(3):12-18(in Chinese)
[8]谢雄,吴冲,陈波林.不同净水措施生产的自来水致突变性研究[J].癌变·畸变·突变,1991,99(1):37-40Xie X,Wu C,Chen B L.Study on the mutagenicity of drinking waters produced from different purifying methods[J].Carcinogenesis,Teratogenesis and Mutagenesis,1991,99(1):37-40(in Chinese)
[9]Sakamoto H,Hayatsu H.A simple method for monitoring mutagenicity of river water.Mutagens in Yodo River System Kyoto-Osaka,Japan[J].Bulletin of Environmental Contamination and Toxicology,1990,44:521-528
[10]Cerna M,Pastorkova A,Smid J,et al.Genotoxicity of industrial effluents,river waters,and their fractions using the Ames test andin vitro cytogenetic assay[J].Toxicology Letters,1996,88:191-197
[11]Magdaleno A,Puig A,Cabo L,et al.Water pollution in an urban argentine river[J].Bulletin of Environmental Contamination and Toxicology,2001,67:408-415
[12]Shen J H,Gutendorf B,Vahl H H,et al.Toxicological profile of pollutants in surface water from an area in Taihu Lake,Yangtze Delta[J].Toxicology,2001,166:71-78
[13]Shen L,Wu J Y,Lin G F,et al.The mutagenic potentials of tap water samples in Shanghai[J].Chemosphere,2003,52:1641-1646
[14]张惠娟,刘爱林,张杰,等.常规处理工艺对饮水中MX及致突变性影响[J].中国公共卫生,2008,24(7):782-784Zhang H J,Liu A L,Zhang J,et.al.Effect of routine water treatment technology on concentrations of MX and mutagenicity of drinking water[J].Chinese Journal of Public Health,2008,24(7):782-784(in Chinese)
[15]邱瑞骥,高健力,哀宝珊,等.自来水不同处理单元出水中有机污染物的致突变性研究[J].中国给水排水,1994,10(2):12-15Qiu R J,Gao J L,Ai B S,et al.Study on the mutagenicity of effluents from different drinking water treatment unit[J].China Water and Wastewater,1994,10(2):12-15(in Chinese)
[16]肖羽堂,许建华.姚江水源及饮用水Ames试验研究[J].环境污染与防治,1998,20(5):19-21Xiao Y T,Xu J H.Ames tests on raw water of Yao River and drinking water[J].Environmental Pollution and Control,1998,20(5):19-21(in Chinese)
[17]唐明德,陈律,易义珍.某饮用水氯化消毒前后不同阶段水样的致突变性研究[J].癌变·畸变·突变,2000,12(3):151-153Tang M D,Chen L,Yi Y Z.The mutagenic study of drinking water in front and behind chlorination of different stage in the water factory[J].Carcinogenesis,Teratogenesis and Mutagenesis,2000,12(3):151-155(in Chinese)
[18]唐非,张水兵,谷康定,等.不同水文期水中非挥发性有机污染物致突变性的比较[J].同济医科大学学报,2001,30(6):541-543Tang F,Zhang S B,Gu K D,et al.Comparison of mutagenicity of nonvolatile organic compounds in water at different hydrographic periods[J].Acta Universitatis Medicinae Tongji,2001,30(6):541-543(in Chinese)
[19]林朝晖.Ames试验在水质检测方面的应用[J].微生物学通报,2002,29(3):66-70Lin Z H.The application of Ames test in the examination of water[J].Microbiology,2002,29(3):66-70(in Chinese)
[20]舒为群,田怀军,曹佳,等.长江、嘉陵江(重庆段)源水有机提取物的致突变活性及其季节变化[J].环境污染与防治,2002,24(4):226-239Shu W Q,Tian H J,Cao J,et al.Mutagenic activities and their seasonal changes of organic extracts from source water of Yangtze River and Jialing River in Chongqing section[J].Environmental Pollution and Control,2002,24(4):226-239(in Chinese)
[21]吴南翔,李新伟,陶核,等.饮用水深度处理过程中有机提取物的致突变性[J].环境与健康杂志,2008,25(2):107-111Wu N X,Li X W,Tao H,et al.Removal of organic mutagen in tap water[J].Journal of Environment and Health,2008,25(2):107-111(in Chinese)
[22]于德奎,朱柏华,韩树清.降低饮水致突变性效果的研究[J].中国给水排水,1994,10(1):29-33Yu D K,Zhu B H,Han S Q.Study on reducing the mutagenicity of drinking water[J].China Water and Wastewater,1994,10(1):29-33(in Chinese)
[23]尚炜,王晓燕,马梅.北京饮用水处理流程中有机提取物致突变性的Ames试验研究[J].首都师范大学学报:自然科学版,2000,21(2):83-87Shang W,Wang X Y,Ma M.Ames test on the mutagenicity of organic extractions of water samples from drinking water treatment processes in Beijing[J].Journal of Capital Normal University:Natural Science Edition,2000,21(2):83-87(in Chinese)
[24]王家玲,运洛咖,郑红俭,等.D湖水中有机污染物致突变性的研究[J].环境科学,1985,6(1):2-5Wang J L,Yun L K,Zheng H J,et al.Study on the mutagenicity of organic pollutants in D Lake[J].Environmental Science,1985,6(1):2-5(in Chinese)
[25]徐凤丹,修瑞琴,周世伟,等.饮用水有机致突变组分的化学分离及其致突变性鉴定[J].环境科学,1985,6(1):5-7Xu F D,Xiu R Q,Zhou S W,et al.Chemical separation and mutagenicity identification of organic mutagenic components in drinking water[J].Environmental Science,1985,6(1):5-7(in Chinese)
[26]聂岚,朱惠刚,蒋颂辉.黄浦江水质致突变性研究[J].上海环境科学,1992,1(4):14-16Nie L,Zhu H G,Jiang S H.Study on the mutagenicity of Huangpu River water[J].Shanghai Environmental Science,1992,1(4):14-16(in Chinese)
[27]张淑琪,王根凤,王占生.氯化消毒对自来水致突变性影响研究[J].癌变·畸变·突变,1999,11(5):224-226Zhang S Q,Wang G F,Wang Z S.The genetic toxicological effect of chlorinating disinfection on drinking water[J].Carcinogenesis,Teratogenesis&Mutagenesis,1999,11(5):224-226(in Chinese)
[28]张青碧,陈志群,甘仲霖,等.Ames试验在泸州市饮水致突变性检测中的应用[J].泸州医学院学报,2003,26(6):495-497Zhang Q B,Chen Z Q,Gan Z L,et al.Application of A-mes test in the detection of mutagenicity of drinking water in Luzhou[J].Journal of Luzhou Medical College,2003,26(6):495-497(in Chinese)
[29]杨玉楠,王宝贞,范延臻,等.O3/AC联用去除水中微污染及致突变物质[J].中国给水排水,2003,19(5):87-88Yang Y N,Wang B Z,Fan Y Z,et al.Removal of micro pollutants and mutagenic substances in water by O3/AC[J].China Water and Wastewater,2003,19(5):87-88(in Chinese)
[30]宋瑞霞,徐凤丹,张秀领,等.上海市饮水重要污染源有机致突变性的动态研究[J].卫生研究,1997,26(5):301-305Song R X,Xu F D,Zhang X L,et.al.Dynamic study on the mutagenic activity of organic concentration of water from one branch port of Huangpu River[J].Journal of Hygiene Research,1997,26(5):301-305(in Chinese)
[31]卢新亚,李倩如,胡军,等.某地区饮用水及水源水致突变性研究[J].河南职工医学院学报,2002,14(1):13-16Lu X Y,Li Q R,Hu J,et al.The mutagenic study of drinking water and source water in the region[J].Journal of Henan Medical College for Staff and Workers,2002,14(1):13-16(in Chinese)
[32]马军,李圭白,陈忠林,等.氯化消毒对水中有机物及水的致突变活性的影响[J].哈尔滨建筑大学学报,1996,29(5):64-67Ma J,Li G B,Chen Z L,et al.Effect of chlorination on organic matters and the mutagenic activity of water[J].Journal of Harbin University of Civil Engineering and Architecture,1996,29(5):64-67(in Chinese)
[33]杨光红,张爱华,王士然,等.常规水处理工艺对地表水有机提取物遗传毒性的影响[J].环境与健康杂志,2013,30(9):799-801Yang G H,Zhang A H,Wang S R,et al.Influence on genotoxicity of organic extracts in surface water by conventional water treatment process[J].Journal of Environment and Health,2013,30(9):799-801(in Chinese)
[34]陈友岚,李树苑.预处理和深度处理对微污染水库水净化的影响研究[J].中国农学通报,2014,30(11):191-195Chen Y L,Li S Y.Influence of pretreatment and advanced treatment on purification treatment of polluted source water[J].Chinese Agricultural Science Bulletin,2014,30(11):191-195(in Chinese)
[35]吴南翔,陶核,杨叶,等.钱塘江水有机提取物对斑马鱼的胚胎毒性[J].环境与职业医学,2016,33(7):698-702Wu N X,Tao H,Yang Y,et al.Toxicity of organic extracts in water from Qiantang River to zebrafish embryos[J].Journal of Environmental and Occupational Medicine,2016,33(7):698-702(in Chinese)
[36]Takeshi O,Tetsushi W,Keiji W.Mutagens in surface waters:A review[J].Mutation Research,2004,567:109-149
[37]陈波林,周晓蓉,邱向红,等.煮沸对自来水有机浓集物致突变性的影响[J].环境与健康杂志,1992,9(3):97-100Chen B L,Zhou X R,Qiu X H,et al.A study on the change of mutagenicity of organic concentrates from tap water treated by boiling[J].Journal of Environment and Health,1992,9(3):97-100(in Chinese)
[38]吴为中,王占生,黄晓东,等.南方某市水源水、自来水的致突变活性现状与改善对策[J].中国环境监测,1998,14(6):45-48Wu W Z,Wang Z S,Huang X D,et al.Current mutagenicity status and its improving decisions of drinking water and source water in a southern city[J].Environmental Monitoring in China,1998,14(6):45-48(in Chinese)
[39]周立红,张淑琪,韩飞,等.不同组合净水工艺对水中致突变物的去除[J].环境科学,1999,20:43-46Zhou L H,Zhang S Q,Han F,et al.A study on the removal of mutagens from water by various united water purification processes[J].Environmental Science,1999,20:43-46(in Chinese)
[40]侯立安,左莉,郭珍珍.反渗透和纳滤工艺对饮用水中致突变物去除的试验研究[J].净水技术,2001,20(4):14-15Hou L A,Zuo L,Guo Z Z.Experiments on the removal of mutagens in the drinking water with RO and Na F[J].Water Purification Technology,2001,20(4):14-15(in Chinese)
[41]龙小庆,王占生.活性炭-纳滤膜工艺去除饮用水中总有机碳和Ames致突变物[J].环境科学,2001,22(1):75-77Long X Q,Wang Z S.Removal of TOC and Ames mutagens from drinking water using AC-NF process[J].Environmental Science,2001,22(1):75-77(in Chinese)
[42]高庆然,周洁.饮用水投加二氧化氯处理后Ames试验研究[J].工业用水与废水,2001,32(6):19-21Gao Q R,Zhou J.Research on Ames addition to the drinking water treated with chlorine dioxide[J].Industrial Water&Wastewater,2001,32(6):19-21(in Chinese)
[43]张旭东,王秀丽,李嫡.引黄原水致突性试验[J].净水技术,2003,22(4):19-21Zhang X D,Wang X L,Li D.Experiment on the mutagenicity of the Yellow River water[J].Water Purification Technology,2003,22(4):19-21(in Chinese)
[44]张崇华,闫海,王勇,等.饮用水有机提取物遗传毒性的研究[J].中国公共卫生管理,2015,31(5):730-731Zhang C H,Yan H,Wang Y,et al.Study on the genotoxicity of organic extracts from drinking water[J].Chinese Journal of Public Health Management,2015,31(5):730-731(in Chinese)
[45]金志玉,马松科,王明树.昆明市某水厂自来水致突变性研究[J].癌变·畸变·突变,1991,3(4):30-39Jin Z Y,Ma S K,Wang M S.Study on the mutagenicity of tap water in a water plant in Kunming[J].Carcinogenesis,Teratogenesis&Mutagenesis,1991,3(4):30-39(in Chinese)
[46]徐凤丹,周世伟,范美云,等.浓集饮水致突变物程序规范化的研究[J].中国公共卫生学报,1993,12(2):84-87Xu F D,Zhou S W,Fan M Y,et al.Study on the standardization of concentrating procedures for mutagens in drinking water[J].Chinese Journal of Public Health,1993,12(2):84-87(in Chinese)
[47]钱黎明,林炜华,王伟彤,等.用Ames试验和SOS显色法研究水源水有机提取物的遗传毒性[J].癌变·畸变·突变,1994,6(1):25-31Qian L M,Lin W H,Wang W T,et al.Study on the genotoxicity of organic extracts from source water by Ames test and SOS coloration method[J].Carcinogenesis,Teratogenesis&Mutagenesis,1994,6(1):25-31(in Chinese)
[48]丁文兴,蒋颂辉,姚加钦.长江水质致突变性研究[J].上海环境科学,1994,13(9):9-12Ding W X,Jiang S H,Yao J Q.Study on mutagenicity of water in Changjiang[J].Shanghai Environmental Sciences,1994,13(9):9-12(in Chinese)
[49]王新为,袁秀文,李君文,等.海河水中有机污染物的致突变性研究[J].中国公共卫生,1999,15(11):975-976Wang X W,Yuan X W,Li J W,et al.Study on the mutagenicity of organic pollutants in Haihe[J].Chinese Journal of Public Health,1999,15(11):975-976(in Chinese)
[50]杜雪飞,谢国祥,贾力敏.南京市饮用水Ames试验结果分析[J].预防医学文献信息,2000,6(1):42-43Du X F,Xie G X,Jia L M.Analysis of Ames test results of drinking water in Nanjing[J].Literature and Information on Preventive Medicine,2000,6(1):42-43(in Chinese)
[51]方东,梅卓华,楼霄,等.用Ames试验检测水源水和自来水中的遗传毒性[J].环境监测管理与技术,2000,12(1):20-22Fang D,Mei Z H,Lou X,et al.Determination of genetic toxicity of source water and drinking water by Ames test[J].The Administration and Technique of Environmental Monitoring,2000,12(1):20-22(in Chinese)
[52]马梅,赵慧芬,王子健,等.官厅水库P永定河水中有机污染物致突变性的初步研究[J].卫生研究,2001,30(6):355-357Ma M,Zhao H F,Wang Z J,et al.Survey on the microbial pollution of pure water in water dispenser and its disinfection[J].Journal of Hygiene Research,2001,30(6):355-357(in Chinese)
[53]吴南翔,杨寅楣,金锋,等.Z流域水质致突变性研究[J].环境与健康杂志,2001,18(4):227-230Wu N X,Yang Y M,Jin F,et al.Study on mutagenicity of surface water in Z valley[J].Journal of Environment and Health,2001,18(4):227-230(in Chinese)
[54]岳舜琳.给水中有机物与Ames致突物的相关性[J].中国给水排水,2003,19(1):20-22Yue S L.Correlation between organic matters and Ames mutagens in water supply[J].China Water and Wastewater,2003,19(1):20-22(in Chinese)
[55]李绍峰,王丽,黄君礼.饮用水中有机物和Ames致突变物的去除[J].哈尔滨工业大学学报,2003,35(12):1534-1536Li S F,Wang L,Huang J L.Removal of organic substances and Ames mutagens from drinking water using both O3+AC and membranes process[J].Journal of Harbin Institute of Technology,2003,35(12):1534-1536(in Chinese)
[56]高乃云,徐斌,芮旻,等.不同预氧化工艺对饮用水致突变活性的影响[J].同济大学学报:自然科学版,2005,33(1):63-67Gao N Y,Xu B,Rui M,et al.Effect on mutagenic activity of drinking water by different pre-oxidation process[J].Journal of Tongji University:Nature Science,2005,33(1):63-67(in Chinese)
[57]郑京哲,杨晓明,董小蓉,等.武汉市水源水及其自来水中有机物致突变性研究[J].公共卫生与预防医学,2008,19(4):9-11Zheng J Z,Yang X M,Dong X R,et al.Study on the mutagenicity of organic substances in source and tap water of Wuhan City[J].Journal of Public Health and Preventive Medicine,2008,19(4):9-11(in Chinese)
[58]金顺亮,陶核,项橘香,等.余杭区农村饮用水卫生现况及致突变性研究[J].浙江预防医学,2009,21(4):4-6Jin S L,Tao H,Xiang J X,et al.Study on hygienic status and mutagenicity of drinking water in Yu Hang rural areas[J].Zhejiang Preventive Medicine,2009,21(4):4-6(in Chinese)
[59]冯春艳,鞠玉娟,王红,等.大庆林源地下水有机浓集物Ames试验结果[J].职业与健康,2009,25(13):1399-1400Feng C Y,Ju Y J,Wang H,et al.Ames test results of organic extracts in ground water of Daqing Linyuan[J].Occupation and Health,2009,25(13):1399-1400(in Chinese)
[60]林炜华,钱黎明.供水工程水源水遗传毒性研究[J].人民珠江,1994(3):44-46Lin W H,Qian L M.A research on the inherited toxicity of water source of a water supply project[J].Pearl River,1994(3):44-46(in Chinese)
[61]聂岚,朱惠刚,蒋项辉.黄浦江上游水源水致突变性研究[J].中国公共卫生学报,1994,13(2):93-95Nie L,Zhu H G,Jiang X H.Study on mutagenicity of source water from upstream of Huangpu River[J].Chinese Journal of Public Health,1994,13(2):93-95(in Chinese)
[62]徐凤丹,周世伟,宋瑞霞,等.我国若干城市饮水的致突变性表征[J].癌变·畸变·突变,1993,5(6):23-24Xu F D,Zhou S W,Song R X,et al.Characterization of mutagenicity of drinking water in some cities in China[J].Carcinogenesis,Teratogenesis and Mutagenesis,1993,5(6):23-24(in Chinese)
[2]许宁,杨璐璐,董梦萌,等.4种消化道肿瘤与饮用水关系的生存分析[J].环境与健康杂志,2015,32(3):231-234Xu N,Yang L L,Dong M M,et al.Survival analysis of four gastrointestinal tumors and drinking shallow groundwater in a basin in middle China[J].Journal of Environment and Health,2015,32(3):231-234(in Chinese)
[3]Fenster L,Waller K,Windhmn G,et a1.Trihalomethane levels in home tap water and semen quality[J].Epidemiology,2003,14:650-658
[4]朱惠刚.水中有机致突变物综合评价指标探讨[J].上海环境科学,1995,14(10):44-47Zhu H G.Comprehensive evaluation index of organic mutagens in water[J].Shanghai Environmental Sciences,1995,14(10):44-47(in Chinese)
[5]张建超.检测水体遗传毒性的短期遗传毒性试验方法[J].科技信息,2013,11:88-89Zhang J C.Widely used short-term genotoxicity assays in water detection[J].Science and Technology Information,2013,11:88-89(in Chinese)
[6]王占生,刘文君,张锡辉.微污染水源饮用水处理[M].北京:中国建筑工业出版社,2016:32-34,280-282,295-297Wang Z S,Liu W J,Zhang X H.Treatment of Micro-polluted Source Water for Drinking[M].Beijing:China Architecture&Building Press,2016:32-34,280-282,295-297(in Chinese)
[7]刘世良,张惠娟,何尚浦,等.自来水有机提取物致突变试验及强致突变物的分离与鉴定[J].湖北预防医学杂志,1994,5(3):12-18Liu S L,Zhang H J,He S P,et al.Mutagenicity test of organic extracts from tap water and isolation and identification of strong mutagens[J].Hubei Journal of Preventive Medicine,1994,5(3):12-18(in Chinese)
[8]谢雄,吴冲,陈波林.不同净水措施生产的自来水致突变性研究[J].癌变·畸变·突变,1991,99(1):37-40Xie X,Wu C,Chen B L.Study on the mutagenicity of drinking waters produced from different purifying methods[J].Carcinogenesis,Teratogenesis and Mutagenesis,1991,99(1):37-40(in Chinese)
[9]Sakamoto H,Hayatsu H.A simple method for monitoring mutagenicity of river water.Mutagens in Yodo River System Kyoto-Osaka,Japan[J].Bulletin of Environmental Contamination and Toxicology,1990,44:521-528
[10]Cerna M,Pastorkova A,Smid J,et al.Genotoxicity of industrial effluents,river waters,and their fractions using the Ames test andin vitro cytogenetic assay[J].Toxicology Letters,1996,88:191-197
[11]Magdaleno A,Puig A,Cabo L,et al.Water pollution in an urban argentine river[J].Bulletin of Environmental Contamination and Toxicology,2001,67:408-415
[12]Shen J H,Gutendorf B,Vahl H H,et al.Toxicological profile of pollutants in surface water from an area in Taihu Lake,Yangtze Delta[J].Toxicology,2001,166:71-78
[13]Shen L,Wu J Y,Lin G F,et al.The mutagenic potentials of tap water samples in Shanghai[J].Chemosphere,2003,52:1641-1646
[14]张惠娟,刘爱林,张杰,等.常规处理工艺对饮水中MX及致突变性影响[J].中国公共卫生,2008,24(7):782-784Zhang H J,Liu A L,Zhang J,et.al.Effect of routine water treatment technology on concentrations of MX and mutagenicity of drinking water[J].Chinese Journal of Public Health,2008,24(7):782-784(in Chinese)
[15]邱瑞骥,高健力,哀宝珊,等.自来水不同处理单元出水中有机污染物的致突变性研究[J].中国给水排水,1994,10(2):12-15Qiu R J,Gao J L,Ai B S,et al.Study on the mutagenicity of effluents from different drinking water treatment unit[J].China Water and Wastewater,1994,10(2):12-15(in Chinese)
[16]肖羽堂,许建华.姚江水源及饮用水Ames试验研究[J].环境污染与防治,1998,20(5):19-21Xiao Y T,Xu J H.Ames tests on raw water of Yao River and drinking water[J].Environmental Pollution and Control,1998,20(5):19-21(in Chinese)
[17]唐明德,陈律,易义珍.某饮用水氯化消毒前后不同阶段水样的致突变性研究[J].癌变·畸变·突变,2000,12(3):151-153Tang M D,Chen L,Yi Y Z.The mutagenic study of drinking water in front and behind chlorination of different stage in the water factory[J].Carcinogenesis,Teratogenesis and Mutagenesis,2000,12(3):151-155(in Chinese)
[18]唐非,张水兵,谷康定,等.不同水文期水中非挥发性有机污染物致突变性的比较[J].同济医科大学学报,2001,30(6):541-543Tang F,Zhang S B,Gu K D,et al.Comparison of mutagenicity of nonvolatile organic compounds in water at different hydrographic periods[J].Acta Universitatis Medicinae Tongji,2001,30(6):541-543(in Chinese)
[19]林朝晖.Ames试验在水质检测方面的应用[J].微生物学通报,2002,29(3):66-70Lin Z H.The application of Ames test in the examination of water[J].Microbiology,2002,29(3):66-70(in Chinese)
[20]舒为群,田怀军,曹佳,等.长江、嘉陵江(重庆段)源水有机提取物的致突变活性及其季节变化[J].环境污染与防治,2002,24(4):226-239Shu W Q,Tian H J,Cao J,et al.Mutagenic activities and their seasonal changes of organic extracts from source water of Yangtze River and Jialing River in Chongqing section[J].Environmental Pollution and Control,2002,24(4):226-239(in Chinese)
[21]吴南翔,李新伟,陶核,等.饮用水深度处理过程中有机提取物的致突变性[J].环境与健康杂志,2008,25(2):107-111Wu N X,Li X W,Tao H,et al.Removal of organic mutagen in tap water[J].Journal of Environment and Health,2008,25(2):107-111(in Chinese)
[22]于德奎,朱柏华,韩树清.降低饮水致突变性效果的研究[J].中国给水排水,1994,10(1):29-33Yu D K,Zhu B H,Han S Q.Study on reducing the mutagenicity of drinking water[J].China Water and Wastewater,1994,10(1):29-33(in Chinese)
[23]尚炜,王晓燕,马梅.北京饮用水处理流程中有机提取物致突变性的Ames试验研究[J].首都师范大学学报:自然科学版,2000,21(2):83-87Shang W,Wang X Y,Ma M.Ames test on the mutagenicity of organic extractions of water samples from drinking water treatment processes in Beijing[J].Journal of Capital Normal University:Natural Science Edition,2000,21(2):83-87(in Chinese)
[24]王家玲,运洛咖,郑红俭,等.D湖水中有机污染物致突变性的研究[J].环境科学,1985,6(1):2-5Wang J L,Yun L K,Zheng H J,et al.Study on the mutagenicity of organic pollutants in D Lake[J].Environmental Science,1985,6(1):2-5(in Chinese)
[25]徐凤丹,修瑞琴,周世伟,等.饮用水有机致突变组分的化学分离及其致突变性鉴定[J].环境科学,1985,6(1):5-7Xu F D,Xiu R Q,Zhou S W,et al.Chemical separation and mutagenicity identification of organic mutagenic components in drinking water[J].Environmental Science,1985,6(1):5-7(in Chinese)
[26]聂岚,朱惠刚,蒋颂辉.黄浦江水质致突变性研究[J].上海环境科学,1992,1(4):14-16Nie L,Zhu H G,Jiang S H.Study on the mutagenicity of Huangpu River water[J].Shanghai Environmental Science,1992,1(4):14-16(in Chinese)
[27]张淑琪,王根凤,王占生.氯化消毒对自来水致突变性影响研究[J].癌变·畸变·突变,1999,11(5):224-226Zhang S Q,Wang G F,Wang Z S.The genetic toxicological effect of chlorinating disinfection on drinking water[J].Carcinogenesis,Teratogenesis&Mutagenesis,1999,11(5):224-226(in Chinese)
[28]张青碧,陈志群,甘仲霖,等.Ames试验在泸州市饮水致突变性检测中的应用[J].泸州医学院学报,2003,26(6):495-497Zhang Q B,Chen Z Q,Gan Z L,et al.Application of A-mes test in the detection of mutagenicity of drinking water in Luzhou[J].Journal of Luzhou Medical College,2003,26(6):495-497(in Chinese)
[29]杨玉楠,王宝贞,范延臻,等.O3/AC联用去除水中微污染及致突变物质[J].中国给水排水,2003,19(5):87-88Yang Y N,Wang B Z,Fan Y Z,et al.Removal of micro pollutants and mutagenic substances in water by O3/AC[J].China Water and Wastewater,2003,19(5):87-88(in Chinese)
[30]宋瑞霞,徐凤丹,张秀领,等.上海市饮水重要污染源有机致突变性的动态研究[J].卫生研究,1997,26(5):301-305Song R X,Xu F D,Zhang X L,et.al.Dynamic study on the mutagenic activity of organic concentration of water from one branch port of Huangpu River[J].Journal of Hygiene Research,1997,26(5):301-305(in Chinese)
[31]卢新亚,李倩如,胡军,等.某地区饮用水及水源水致突变性研究[J].河南职工医学院学报,2002,14(1):13-16Lu X Y,Li Q R,Hu J,et al.The mutagenic study of drinking water and source water in the region[J].Journal of Henan Medical College for Staff and Workers,2002,14(1):13-16(in Chinese)
[32]马军,李圭白,陈忠林,等.氯化消毒对水中有机物及水的致突变活性的影响[J].哈尔滨建筑大学学报,1996,29(5):64-67Ma J,Li G B,Chen Z L,et al.Effect of chlorination on organic matters and the mutagenic activity of water[J].Journal of Harbin University of Civil Engineering and Architecture,1996,29(5):64-67(in Chinese)
[33]杨光红,张爱华,王士然,等.常规水处理工艺对地表水有机提取物遗传毒性的影响[J].环境与健康杂志,2013,30(9):799-801Yang G H,Zhang A H,Wang S R,et al.Influence on genotoxicity of organic extracts in surface water by conventional water treatment process[J].Journal of Environment and Health,2013,30(9):799-801(in Chinese)
[34]陈友岚,李树苑.预处理和深度处理对微污染水库水净化的影响研究[J].中国农学通报,2014,30(11):191-195Chen Y L,Li S Y.Influence of pretreatment and advanced treatment on purification treatment of polluted source water[J].Chinese Agricultural Science Bulletin,2014,30(11):191-195(in Chinese)
[35]吴南翔,陶核,杨叶,等.钱塘江水有机提取物对斑马鱼的胚胎毒性[J].环境与职业医学,2016,33(7):698-702Wu N X,Tao H,Yang Y,et al.Toxicity of organic extracts in water from Qiantang River to zebrafish embryos[J].Journal of Environmental and Occupational Medicine,2016,33(7):698-702(in Chinese)
[36]Takeshi O,Tetsushi W,Keiji W.Mutagens in surface waters:A review[J].Mutation Research,2004,567:109-149
[37]陈波林,周晓蓉,邱向红,等.煮沸对自来水有机浓集物致突变性的影响[J].环境与健康杂志,1992,9(3):97-100Chen B L,Zhou X R,Qiu X H,et al.A study on the change of mutagenicity of organic concentrates from tap water treated by boiling[J].Journal of Environment and Health,1992,9(3):97-100(in Chinese)
[38]吴为中,王占生,黄晓东,等.南方某市水源水、自来水的致突变活性现状与改善对策[J].中国环境监测,1998,14(6):45-48Wu W Z,Wang Z S,Huang X D,et al.Current mutagenicity status and its improving decisions of drinking water and source water in a southern city[J].Environmental Monitoring in China,1998,14(6):45-48(in Chinese)
[39]周立红,张淑琪,韩飞,等.不同组合净水工艺对水中致突变物的去除[J].环境科学,1999,20:43-46Zhou L H,Zhang S Q,Han F,et al.A study on the removal of mutagens from water by various united water purification processes[J].Environmental Science,1999,20:43-46(in Chinese)
[40]侯立安,左莉,郭珍珍.反渗透和纳滤工艺对饮用水中致突变物去除的试验研究[J].净水技术,2001,20(4):14-15Hou L A,Zuo L,Guo Z Z.Experiments on the removal of mutagens in the drinking water with RO and Na F[J].Water Purification Technology,2001,20(4):14-15(in Chinese)
[41]龙小庆,王占生.活性炭-纳滤膜工艺去除饮用水中总有机碳和Ames致突变物[J].环境科学,2001,22(1):75-77Long X Q,Wang Z S.Removal of TOC and Ames mutagens from drinking water using AC-NF process[J].Environmental Science,2001,22(1):75-77(in Chinese)
[42]高庆然,周洁.饮用水投加二氧化氯处理后Ames试验研究[J].工业用水与废水,2001,32(6):19-21Gao Q R,Zhou J.Research on Ames addition to the drinking water treated with chlorine dioxide[J].Industrial Water&Wastewater,2001,32(6):19-21(in Chinese)
[43]张旭东,王秀丽,李嫡.引黄原水致突性试验[J].净水技术,2003,22(4):19-21Zhang X D,Wang X L,Li D.Experiment on the mutagenicity of the Yellow River water[J].Water Purification Technology,2003,22(4):19-21(in Chinese)
[44]张崇华,闫海,王勇,等.饮用水有机提取物遗传毒性的研究[J].中国公共卫生管理,2015,31(5):730-731Zhang C H,Yan H,Wang Y,et al.Study on the genotoxicity of organic extracts from drinking water[J].Chinese Journal of Public Health Management,2015,31(5):730-731(in Chinese)
[45]金志玉,马松科,王明树.昆明市某水厂自来水致突变性研究[J].癌变·畸变·突变,1991,3(4):30-39Jin Z Y,Ma S K,Wang M S.Study on the mutagenicity of tap water in a water plant in Kunming[J].Carcinogenesis,Teratogenesis&Mutagenesis,1991,3(4):30-39(in Chinese)
[46]徐凤丹,周世伟,范美云,等.浓集饮水致突变物程序规范化的研究[J].中国公共卫生学报,1993,12(2):84-87Xu F D,Zhou S W,Fan M Y,et al.Study on the standardization of concentrating procedures for mutagens in drinking water[J].Chinese Journal of Public Health,1993,12(2):84-87(in Chinese)
[47]钱黎明,林炜华,王伟彤,等.用Ames试验和SOS显色法研究水源水有机提取物的遗传毒性[J].癌变·畸变·突变,1994,6(1):25-31Qian L M,Lin W H,Wang W T,et al.Study on the genotoxicity of organic extracts from source water by Ames test and SOS coloration method[J].Carcinogenesis,Teratogenesis&Mutagenesis,1994,6(1):25-31(in Chinese)
[48]丁文兴,蒋颂辉,姚加钦.长江水质致突变性研究[J].上海环境科学,1994,13(9):9-12Ding W X,Jiang S H,Yao J Q.Study on mutagenicity of water in Changjiang[J].Shanghai Environmental Sciences,1994,13(9):9-12(in Chinese)
[49]王新为,袁秀文,李君文,等.海河水中有机污染物的致突变性研究[J].中国公共卫生,1999,15(11):975-976Wang X W,Yuan X W,Li J W,et al.Study on the mutagenicity of organic pollutants in Haihe[J].Chinese Journal of Public Health,1999,15(11):975-976(in Chinese)
[50]杜雪飞,谢国祥,贾力敏.南京市饮用水Ames试验结果分析[J].预防医学文献信息,2000,6(1):42-43Du X F,Xie G X,Jia L M.Analysis of Ames test results of drinking water in Nanjing[J].Literature and Information on Preventive Medicine,2000,6(1):42-43(in Chinese)
[51]方东,梅卓华,楼霄,等.用Ames试验检测水源水和自来水中的遗传毒性[J].环境监测管理与技术,2000,12(1):20-22Fang D,Mei Z H,Lou X,et al.Determination of genetic toxicity of source water and drinking water by Ames test[J].The Administration and Technique of Environmental Monitoring,2000,12(1):20-22(in Chinese)
[52]马梅,赵慧芬,王子健,等.官厅水库P永定河水中有机污染物致突变性的初步研究[J].卫生研究,2001,30(6):355-357Ma M,Zhao H F,Wang Z J,et al.Survey on the microbial pollution of pure water in water dispenser and its disinfection[J].Journal of Hygiene Research,2001,30(6):355-357(in Chinese)
[53]吴南翔,杨寅楣,金锋,等.Z流域水质致突变性研究[J].环境与健康杂志,2001,18(4):227-230Wu N X,Yang Y M,Jin F,et al.Study on mutagenicity of surface water in Z valley[J].Journal of Environment and Health,2001,18(4):227-230(in Chinese)
[54]岳舜琳.给水中有机物与Ames致突物的相关性[J].中国给水排水,2003,19(1):20-22Yue S L.Correlation between organic matters and Ames mutagens in water supply[J].China Water and Wastewater,2003,19(1):20-22(in Chinese)
[55]李绍峰,王丽,黄君礼.饮用水中有机物和Ames致突变物的去除[J].哈尔滨工业大学学报,2003,35(12):1534-1536Li S F,Wang L,Huang J L.Removal of organic substances and Ames mutagens from drinking water using both O3+AC and membranes process[J].Journal of Harbin Institute of Technology,2003,35(12):1534-1536(in Chinese)
[56]高乃云,徐斌,芮旻,等.不同预氧化工艺对饮用水致突变活性的影响[J].同济大学学报:自然科学版,2005,33(1):63-67Gao N Y,Xu B,Rui M,et al.Effect on mutagenic activity of drinking water by different pre-oxidation process[J].Journal of Tongji University:Nature Science,2005,33(1):63-67(in Chinese)
[57]郑京哲,杨晓明,董小蓉,等.武汉市水源水及其自来水中有机物致突变性研究[J].公共卫生与预防医学,2008,19(4):9-11Zheng J Z,Yang X M,Dong X R,et al.Study on the mutagenicity of organic substances in source and tap water of Wuhan City[J].Journal of Public Health and Preventive Medicine,2008,19(4):9-11(in Chinese)
[58]金顺亮,陶核,项橘香,等.余杭区农村饮用水卫生现况及致突变性研究[J].浙江预防医学,2009,21(4):4-6Jin S L,Tao H,Xiang J X,et al.Study on hygienic status and mutagenicity of drinking water in Yu Hang rural areas[J].Zhejiang Preventive Medicine,2009,21(4):4-6(in Chinese)
[59]冯春艳,鞠玉娟,王红,等.大庆林源地下水有机浓集物Ames试验结果[J].职业与健康,2009,25(13):1399-1400Feng C Y,Ju Y J,Wang H,et al.Ames test results of organic extracts in ground water of Daqing Linyuan[J].Occupation and Health,2009,25(13):1399-1400(in Chinese)
[60]林炜华,钱黎明.供水工程水源水遗传毒性研究[J].人民珠江,1994(3):44-46Lin W H,Qian L M.A research on the inherited toxicity of water source of a water supply project[J].Pearl River,1994(3):44-46(in Chinese)
[61]聂岚,朱惠刚,蒋项辉.黄浦江上游水源水致突变性研究[J].中国公共卫生学报,1994,13(2):93-95Nie L,Zhu H G,Jiang X H.Study on mutagenicity of source water from upstream of Huangpu River[J].Chinese Journal of Public Health,1994,13(2):93-95(in Chinese)
[62]徐凤丹,周世伟,宋瑞霞,等.我国若干城市饮水的致突变性表征[J].癌变·畸变·突变,1993,5(6):23-24Xu F D,Zhou S W,Song R X,et al.Characterization of mutagenicity of drinking water in some cities in China[J].Carcinogenesis,Teratogenesis and Mutagenesis,1993,5(6):23-24(in Chinese)