不同风信子品种杂交亲和性初探
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摘要
本研究以17个品种风信子为试验材料,设置不同品种间杂交组合191个,自交组合7个,通过子房膨大率、坐果率、平均种子数判定不同杂交组合的杂交亲和性。结果显示:191个杂交组合中有15个组合子房膨大率和坐果率均大于80%,杂交亲和性较高,有40个组合存在受精前后障碍,未获得杂交后代。其中蓝色系的‘Ostara’和‘Sky Jacket’无论是做母本还是父本,比其他品种的杂交亲和性都相对较高;白色系的‘White Pearl’和粉色系的‘Lady Derby’适合做母本;橘黄色系的‘Gipsy Queen’和粉色系的‘Anna Marie’适合做父本。7个自交组合中只有‘Gipsy Queen’和‘Carnegie’获得了杂交后代,其余均存在自交不亲和现象。
In this study, 17 varieties of hyacinths were used as test materials, 191 hybrid combinations were set among different varieties, and 7 were self-cross combinations. The cross compatibility of different hybrid combinations was determined by the rate of ovary enlargement, fruit setting rate and average seed number. The results showed that 15 of the 191 hybrid combinations had ovary enlargement rate and fruit setting rate of more than 80%,and the hybridization affinity was high. 40 combinations had pre-and post-fertilization barriers and no hybrid progeny. Among them, the blue 'Ostara' and 'Sky Jacket', whether maternal or paternal, have higher hybridization affinities than other species; white 'White Pearl' and pink 'Lady Derby' are suitable for female parent; orange 'Gipsy Queen' and pink 'Anna Marie' are suitable for male parent. Only the 'Gipsy Queen' and 'Carnegie' got hybrids among the 7 self-crossing combinations, and the rest had self-incompatibility.
In this study, 17 varieties of hyacinths were used as test materials, 191 hybrid combinations were set among different varieties, and 7 were self-cross combinations. The cross compatibility of different hybrid combinations was determined by the rate of ovary enlargement, fruit setting rate and average seed number. The results showed that 15 of the 191 hybrid combinations had ovary enlargement rate and fruit setting rate of more than 80%,and the hybridization affinity was high. 40 combinations had pre-and post-fertilization barriers and no hybrid progeny. Among them, the blue 'Ostara' and 'Sky Jacket', whether maternal or paternal, have higher hybridization affinities than other species; white 'White Pearl' and pink 'Lady Derby' are suitable for female parent; orange 'Gipsy Queen' and pink 'Anna Marie' are suitable for male parent. Only the 'Gipsy Queen' and 'Carnegie' got hybrids among the 7 self-crossing combinations, and the rest had self-incompatibility.
引文
Cai M.,Wang X.Y.,Zhang Q.X.,Pan H.T.,and Wang X.F.,2010,Compatibility of interspecific crosses between Lagerstroemia indica cultivars and Lagerstroemia caudata,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),30(4):697-701(蔡明,王晓玉,张启翔,潘会堂,王学凤,2010,紫薇品种与尾叶紫薇种间杂交亲和性研究,西北植物学报,30(4):697-701)
Cheng X.,Chen S.M.,Chen F.D.,Fang W.M.,Deng Y.M.,and She L.F.,2010,Interspecific hybrids between Dendranthema morifolium(Ramat.)Kitamura and D.nankingense(Nakai)Tzvel.achieved using ovary rescue and their cold tolerance characteristics,Euphytica,172(1):101-108
Hang J.M.,1982,Cultvation of hybrid from Wang Lili X David Lily,Yuanyi Xuebao(Acta Horticulturae Sinica),9(3):51-55(黄济明,1982,王百合与大卫百合种间远缘杂交杂种的育成,园艺学报,9(3):51-55)
Jiao X.H.,Wu J.D.,Diao Y.W.,Wu S.S.,Zhang Y.J.,and LüY.M.,2010,Study on the cross compatibility of asiatic hybrid lily cultivars,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),8(5):933-938(焦雪辉,吴锦娣,刁义维,吴沙沙,张英杰,吕英民,2010,亚洲百合杂交亲和性研究,分子植物育种,8(5):933-938)
Li F.T.,Chen X.L.,Liu C.G.,Sun Y.,Ma H.,Zhang T.,and Bao J.Z.,2012,Effects of different nutrient solutions on growth and ornamental quality of hydroponic Hyacinthus orientalis L.,Zhongnan Linye Keji Daxue Xuebao(Journal of Central South University of Forestry&Technology),32(10):130-134(李风童,陈秀兰,刘春贵,孙叶,马辉,张甜,包建忠,2012,不同配方营养液对水培风信子生长及观赏品质的影响,中南林业科技大学学报,32(10):130-134)
Li X.L.,Chen F.D.,and Zhao H.B.,2008,Compatibility of interspecific cross in Dendranthema genus,Yuanyi Xuebao(Acta Horticulturae Sinica),35(2):257-262(李辛雷,陈发棣,赵宏波,2008,菊属植物远缘杂交亲和性研究,园艺学报,35(2):257-262)
Li Y.P.,2011,Studies on the reproductive biology of Hyacinthus orientalis L.,Dissertation for Ph.D.,Nanjing Forestry University,Supervisor:Tang G.G.,pp.67-80(李玉萍,2011,风信子(Hyacinthus orientalis L.)繁殖生物学研究,博士学位论文,南京林业大学,导师:汤庚国,pp.67-80)
Liu C.,Mu D.,Ming J.,and Dong L.,2006,The pre-fertilization barriers research of Lilium interspecific hybridization,Yuanyi Xuebao(Acta Horticulturae Sinica),33(3):653-656(刘春,穆鼎,明军,董玲,2006,百合种间杂交受精前障碍的研究,园艺学报,33(3):653-656)
Luo J.R.,Zhang Y.L.,and Zhang L.H.,2007,The pilot study of the methods to overcome self-incompatibility and cross-incompatibility in lily,Xibei Nongye Xuebao(Acta Agriculturae Boreali-Occidentalis Sinica),16(4):260-263(罗建让,张延龙,张林华,2007,克服百合自交及杂交障碍方法的初步研究,西北农业学报,16(4):260-263)
Peng X.L.,Liao K.,Jia Y.,Liu H.,Ma W.,and Xu L.,2015,Study on the cross compatibility among 9 apricot cultivars in Xinjiang,Guoshu Xuebao(Journal of Fruit Science),32(2):192-199(彭晓莉,廖康,贾杨,刘欢,马微,徐乐,2015,9个新疆杏品种间杂交亲和性研究,果树学报,32(2):192-199)
Qu Y.H.,Xiong L.,Wu X.W.,and Wang Q.G.,2007,Effect of different genotype crossing on embryo rescue of lily,Xinan Nongye Xuebao(Southwest China Journal of Agricultural Sciences),20(4):711-715(屈云慧,熊丽,吴学尉,王其刚,2007,百合不同远缘杂交基因型对胚培救效果的影响,西南农业学报,20(4):711-715)
Wang C.L.,Chen F.J.,Jin Y.P.,Wang Y.B.,and Liang H.W.,2015,Flower phenology and breeding system of Catalpa bungei(Bignoniaceae),Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(9):1981-1987(王长兰,陈发菊,金玉佩,王玉兵,梁宏伟,2015,楸树的开花生物学及繁育系统研究,基因组学与应用生物学,34(9):1981-1987)
Wang Z.X.,Yang S.,Lian Y.Q.,Feng J.H.,Wei C.,and Jia G.X.,2012,Cross-compatibillity among some hybrids cuitivars in Lilium,Xibei Nongye Xuebao(Acta Agriculturae Boreali-Occidentalis Sinica),21(9):113-120(王中轩,杨爽,廉玉芹,冯晶红,魏迟,贾桂霞,2012,百合品种间杂交亲和性的比较,西北农业学报,21(9):113-120)
Xu S.C.,Guan J.,Cao H.Y.,and Lv Y.M.,2014,Study on the relationship between lily cross-breeding affinity and ploidy levels,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),12(2):316-322(徐顺超,管洁,曹荷艳,吕英民,2014,百合杂交育种亲和性与染色体倍性相关性研究,分子植物育种,12(2):316-322)
Yang L.P.,Zhang X.F.,Ding B.,and Zhao L.J.,1998,Cultivation of hybrid from Lilium callosum L.regale,Dongbei Linye Daxue Xuebao(Journal of Northeast Forestry University),26(2):37-40(杨利平,张敩方,丁冰,赵立杰,1998,条叶百合×王百合种间杂种的育成,东北林业大学学报,26(2):37-40)
Zhang C.Y.,2012,Study on cross compatibility of Rhododendron L.,Thesis for M.S.,Nanjing Forestry University,Supervisor:Geng X.M.,pp.29-37(张超仪,2012,杜鹃花属植物杂交亲和性研究,硕士学位论文,南京林业大学,导师:耿兴敏,pp.29-37)
Zhang C.C.,2010,Research on breeding and propagation of Hyacinthus orentalis L.,Thesis for M.S.,Shanghai Jiaotong U-niversity,Supervisor:Shi Y.M.,pp.1-43(张成宬,2010,风信子育种与繁殖研究,硕士学位论文,上海交通大学,导师:史益敏,pp.1-43)
Zhang G.X.,and Zhou L.Q.,2013,Effects of SA on the growth and flowering of hydroponics Hyacinthus orientalis,Nanjing Linye Daxue Xuebao(Journal of Nanjing Forestry University)37(5):20-24(张鸽香,周丽琴,2013,水杨酸对水培风信子生长及开花的影响,南京林业大学学报,37(5):20-24)
Zhao H.X.,Wang J.,Ding X.L.,Luo L.,Pan H.T.,and Zhang Q.X.,2015,Compatibility of interspecific crossing between several Rosa species and modern rose cultivars,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),35(4):743-753(赵红霞,王晶,丁晓六,罗乐,潘会堂,张启翔,2015,蔷薇属植物与现代月季品种杂交亲和性研究,西北植物学报,35(4):743-753)
Zhou X.J.,2009,Lily distant hybridization and seed characteristics,Thesis for M.S.,Northeast Agricultural University,Supervisor:Che D.D.,pp.21-61(周晓杰,2009,百合杂交育种及杂交种子特性的研究,硕士学位论文,东北农业大学,导师:车代弟,pp.21-61)
Cheng X.,Chen S.M.,Chen F.D.,Fang W.M.,Deng Y.M.,and She L.F.,2010,Interspecific hybrids between Dendranthema morifolium(Ramat.)Kitamura and D.nankingense(Nakai)Tzvel.achieved using ovary rescue and their cold tolerance characteristics,Euphytica,172(1):101-108
Hang J.M.,1982,Cultvation of hybrid from Wang Lili X David Lily,Yuanyi Xuebao(Acta Horticulturae Sinica),9(3):51-55(黄济明,1982,王百合与大卫百合种间远缘杂交杂种的育成,园艺学报,9(3):51-55)
Jiao X.H.,Wu J.D.,Diao Y.W.,Wu S.S.,Zhang Y.J.,and LüY.M.,2010,Study on the cross compatibility of asiatic hybrid lily cultivars,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),8(5):933-938(焦雪辉,吴锦娣,刁义维,吴沙沙,张英杰,吕英民,2010,亚洲百合杂交亲和性研究,分子植物育种,8(5):933-938)
Li F.T.,Chen X.L.,Liu C.G.,Sun Y.,Ma H.,Zhang T.,and Bao J.Z.,2012,Effects of different nutrient solutions on growth and ornamental quality of hydroponic Hyacinthus orientalis L.,Zhongnan Linye Keji Daxue Xuebao(Journal of Central South University of Forestry&Technology),32(10):130-134(李风童,陈秀兰,刘春贵,孙叶,马辉,张甜,包建忠,2012,不同配方营养液对水培风信子生长及观赏品质的影响,中南林业科技大学学报,32(10):130-134)
Li X.L.,Chen F.D.,and Zhao H.B.,2008,Compatibility of interspecific cross in Dendranthema genus,Yuanyi Xuebao(Acta Horticulturae Sinica),35(2):257-262(李辛雷,陈发棣,赵宏波,2008,菊属植物远缘杂交亲和性研究,园艺学报,35(2):257-262)
Li Y.P.,2011,Studies on the reproductive biology of Hyacinthus orientalis L.,Dissertation for Ph.D.,Nanjing Forestry University,Supervisor:Tang G.G.,pp.67-80(李玉萍,2011,风信子(Hyacinthus orientalis L.)繁殖生物学研究,博士学位论文,南京林业大学,导师:汤庚国,pp.67-80)
Liu C.,Mu D.,Ming J.,and Dong L.,2006,The pre-fertilization barriers research of Lilium interspecific hybridization,Yuanyi Xuebao(Acta Horticulturae Sinica),33(3):653-656(刘春,穆鼎,明军,董玲,2006,百合种间杂交受精前障碍的研究,园艺学报,33(3):653-656)
Luo J.R.,Zhang Y.L.,and Zhang L.H.,2007,The pilot study of the methods to overcome self-incompatibility and cross-incompatibility in lily,Xibei Nongye Xuebao(Acta Agriculturae Boreali-Occidentalis Sinica),16(4):260-263(罗建让,张延龙,张林华,2007,克服百合自交及杂交障碍方法的初步研究,西北农业学报,16(4):260-263)
Peng X.L.,Liao K.,Jia Y.,Liu H.,Ma W.,and Xu L.,2015,Study on the cross compatibility among 9 apricot cultivars in Xinjiang,Guoshu Xuebao(Journal of Fruit Science),32(2):192-199(彭晓莉,廖康,贾杨,刘欢,马微,徐乐,2015,9个新疆杏品种间杂交亲和性研究,果树学报,32(2):192-199)
Qu Y.H.,Xiong L.,Wu X.W.,and Wang Q.G.,2007,Effect of different genotype crossing on embryo rescue of lily,Xinan Nongye Xuebao(Southwest China Journal of Agricultural Sciences),20(4):711-715(屈云慧,熊丽,吴学尉,王其刚,2007,百合不同远缘杂交基因型对胚培救效果的影响,西南农业学报,20(4):711-715)
Wang C.L.,Chen F.J.,Jin Y.P.,Wang Y.B.,and Liang H.W.,2015,Flower phenology and breeding system of Catalpa bungei(Bignoniaceae),Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(9):1981-1987(王长兰,陈发菊,金玉佩,王玉兵,梁宏伟,2015,楸树的开花生物学及繁育系统研究,基因组学与应用生物学,34(9):1981-1987)
Wang Z.X.,Yang S.,Lian Y.Q.,Feng J.H.,Wei C.,and Jia G.X.,2012,Cross-compatibillity among some hybrids cuitivars in Lilium,Xibei Nongye Xuebao(Acta Agriculturae Boreali-Occidentalis Sinica),21(9):113-120(王中轩,杨爽,廉玉芹,冯晶红,魏迟,贾桂霞,2012,百合品种间杂交亲和性的比较,西北农业学报,21(9):113-120)
Xu S.C.,Guan J.,Cao H.Y.,and Lv Y.M.,2014,Study on the relationship between lily cross-breeding affinity and ploidy levels,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),12(2):316-322(徐顺超,管洁,曹荷艳,吕英民,2014,百合杂交育种亲和性与染色体倍性相关性研究,分子植物育种,12(2):316-322)
Yang L.P.,Zhang X.F.,Ding B.,and Zhao L.J.,1998,Cultivation of hybrid from Lilium callosum L.regale,Dongbei Linye Daxue Xuebao(Journal of Northeast Forestry University),26(2):37-40(杨利平,张敩方,丁冰,赵立杰,1998,条叶百合×王百合种间杂种的育成,东北林业大学学报,26(2):37-40)
Zhang C.Y.,2012,Study on cross compatibility of Rhododendron L.,Thesis for M.S.,Nanjing Forestry University,Supervisor:Geng X.M.,pp.29-37(张超仪,2012,杜鹃花属植物杂交亲和性研究,硕士学位论文,南京林业大学,导师:耿兴敏,pp.29-37)
Zhang C.C.,2010,Research on breeding and propagation of Hyacinthus orentalis L.,Thesis for M.S.,Shanghai Jiaotong U-niversity,Supervisor:Shi Y.M.,pp.1-43(张成宬,2010,风信子育种与繁殖研究,硕士学位论文,上海交通大学,导师:史益敏,pp.1-43)
Zhang G.X.,and Zhou L.Q.,2013,Effects of SA on the growth and flowering of hydroponics Hyacinthus orientalis,Nanjing Linye Daxue Xuebao(Journal of Nanjing Forestry University)37(5):20-24(张鸽香,周丽琴,2013,水杨酸对水培风信子生长及开花的影响,南京林业大学学报,37(5):20-24)
Zhao H.X.,Wang J.,Ding X.L.,Luo L.,Pan H.T.,and Zhang Q.X.,2015,Compatibility of interspecific crossing between several Rosa species and modern rose cultivars,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),35(4):743-753(赵红霞,王晶,丁晓六,罗乐,潘会堂,张启翔,2015,蔷薇属植物与现代月季品种杂交亲和性研究,西北植物学报,35(4):743-753)
Zhou X.J.,2009,Lily distant hybridization and seed characteristics,Thesis for M.S.,Northeast Agricultural University,Supervisor:Che D.D.,pp.21-61(周晓杰,2009,百合杂交育种及杂交种子特性的研究,硕士学位论文,东北农业大学,导师:车代弟,pp.21-61)