多波长RP-HPLC法同时测定盆炎净胶囊中绿原酸和芍药苷的含量
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摘要
目的:为有效控制盆炎净胶囊的质量,建立多波长RP-HPLC法同时测定盆炎净胶囊中绿原酸和芍药苷含量的分析方法。方法:仪器为安捷伦1260型高效液相色谱仪,色谱柱为:Kromasil 100-5 C18柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.1%磷酸溶液(12∶88)溶液,绿原酸、芍药苷的检测波长分别为327 nm、230 nm,流速1.0 mL·min~(-1),柱温35℃。结果:在上述色谱条件下,绿原酸、芍药苷分别在0.213 9～1.283 4μg、0.192 9～1.157 4μg范围内,其进样质量与峰面积呈良好的线性关系。回归方程:绿原酸Y=273 3.7X+154.34(r=0.999 2);芍药苷Y=1 306.8X+23.223(r=0.999 6)。绿原酸、芍药苷的平均回收率分别为99.51%、99.62%,RSD分别为0.8%、0.72%。测定样品6批,绿原酸、芍药苷的平均含量分别为0.94 mg·g~(-1)、0.92 mg·g~(-1)。结论:本方法操作简便、准确,灵敏度高、重复性好,可用于盆炎净胶囊中绿原酸和芍药苷的同时测定,为其质量控制提供借鉴。
Objective: To establish a multiple-wavelength RP-HPLC method for the simultaneous determination of chlorogenic acid and paeoniflorin in Penyanjing capsules in order that the quality of Penyanjing capsules can be controlled effectively. Methods: Agilent 1260 high-performance liquid chromatograph was used. The chromatographic separation was performed on a Kromasil 100-5 C18 column(4.6 mm×250 mm, 5 μm) under the elution of acetonitrile-0.1% phosphoric acid aqueous solution(12∶88). The detection wavelengths for chlorogenic acid and paeoniflorin were 327 and 230 nm, respectively. The flow rate was 1.0 mL·min~(-1), and the column temperature was 35 ℃. Results: Chlorogenic acid and paeoniflorin could be separated efficiently under the above chromatographic condition. The linear ranges of chlorogenic acid and paeoniflorin fell into 0.213 9-1.283 4 and 0.192 9-1.157 4 μg, respectively. The regression equations of chlorogenic acid and paeoniflorin were Y=2 733.7X+154.34(r=0.999 2) and Y=1 306.8X+23.223(r=0.999 6), respectively. The average recoveries of chlorogenic acid and paeoniflorin were 99.51% and 99.62% with RSD values 0.8% and 0.72%. The average contents of chlorogenic acid and paeoniflorin in 6 batches of samples were 0.94 mg·g~(-1) and 0.92 mg·g~(-1), respectively. Conclusion: This method was simple, accurate, sensitive and repeatable. It can be used for the simultaneous determination of chlorogenic acid and paeoniflorin in Penyanjing capsules. That can provide reference for the quality control of Penyanjing capsule.
Objective: To establish a multiple-wavelength RP-HPLC method for the simultaneous determination of chlorogenic acid and paeoniflorin in Penyanjing capsules in order that the quality of Penyanjing capsules can be controlled effectively. Methods: Agilent 1260 high-performance liquid chromatograph was used. The chromatographic separation was performed on a Kromasil 100-5 C18 column(4.6 mm×250 mm, 5 μm) under the elution of acetonitrile-0.1% phosphoric acid aqueous solution(12∶88). The detection wavelengths for chlorogenic acid and paeoniflorin were 327 and 230 nm, respectively. The flow rate was 1.0 mL·min~(-1), and the column temperature was 35 ℃. Results: Chlorogenic acid and paeoniflorin could be separated efficiently under the above chromatographic condition. The linear ranges of chlorogenic acid and paeoniflorin fell into 0.213 9-1.283 4 and 0.192 9-1.157 4 μg, respectively. The regression equations of chlorogenic acid and paeoniflorin were Y=2 733.7X+154.34(r=0.999 2) and Y=1 306.8X+23.223(r=0.999 6), respectively. The average recoveries of chlorogenic acid and paeoniflorin were 99.51% and 99.62% with RSD values 0.8% and 0.72%. The average contents of chlorogenic acid and paeoniflorin in 6 batches of samples were 0.94 mg·g~(-1) and 0.92 mg·g~(-1), respectively. Conclusion: This method was simple, accurate, sensitive and repeatable. It can be used for the simultaneous determination of chlorogenic acid and paeoniflorin in Penyanjing capsules. That can provide reference for the quality control of Penyanjing capsule.
引文
[1] 陈叶青.RP-HPLC法测定盆炎净胶囊中绿原酸的含量[J]. 中国药师,2010,8(13):1205CHEN YQ. Determination of chlorogenic acid in Penyan Jing capsule by RP-HPLC[J]. China Pharm, 2010, 8(13):1205
[2] 周海婴,王斯成.HPLC法测定盆炎净胶囊中芍药苷的含量[J]. 中国医药导报,2007,4(2):159ZHOU HY, WANG SC. Determination of paeoniflorin in PenYan Jing capsule by HPLC[J]. Chin Med Her, 2007, 4(2):159
[3] 陈勇,蒋慧莲,周建良,等. HPLC法测定盆炎净胶囊中芍药苷的含量[J]. 西北药学杂志,2013,5(28):492CHEN Y, JIANG HL, ZHOU JL, et al. Determination of paeoniflorin in PenYan Jing capsule by HPLC[J]. Northwest Pharm J, 2013,5(28):492
[4] 袁志鹰,杨岩涛,李荣东,等. HPLC同时测定盆炎净胶囊中3种有效成分含量[J]. 中国中医药信息杂志,2017,5(24):82YUAN ZY, YANG YT, LI RD, et al. Simultaneous determination of 3 effective components in PenYan Jing capsule by HPLC[J]. Chin J Inf Tradit Chin Med, 2017, 5(24):82
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[7] 周海玲,许舜军,杨柳. HPLC法测定赤芍中芍药苷的含量[J]. 临床医学工程,2011,18(4):487ZHOU HL, XU SJ,YANG L. Determination of paeoniflorin in Radix Paeoniae Rubra by HPLC[J]. Clin Med Eng, 2011, 18(4):487
[8] 贾法强,尹宁宁,林林. HPLC法测定忍冬藤中绿原酸的含量[J]. 齐鲁药事,2007,1(26):26JIA FQ, YIN NN, LIN L. Determination of chlorogenic acid in Honeysuckle Stem by HPLC[J]. Qilu Pharm Aff, 2007,1(26):26
[2] 周海婴,王斯成.HPLC法测定盆炎净胶囊中芍药苷的含量[J]. 中国医药导报,2007,4(2):159ZHOU HY, WANG SC. Determination of paeoniflorin in PenYan Jing capsule by HPLC[J]. Chin Med Her, 2007, 4(2):159
[3] 陈勇,蒋慧莲,周建良,等. HPLC法测定盆炎净胶囊中芍药苷的含量[J]. 西北药学杂志,2013,5(28):492CHEN Y, JIANG HL, ZHOU JL, et al. Determination of paeoniflorin in PenYan Jing capsule by HPLC[J]. Northwest Pharm J, 2013,5(28):492
[4] 袁志鹰,杨岩涛,李荣东,等. HPLC同时测定盆炎净胶囊中3种有效成分含量[J]. 中国中医药信息杂志,2017,5(24):82YUAN ZY, YANG YT, LI RD, et al. Simultaneous determination of 3 effective components in PenYan Jing capsule by HPLC[J]. Chin J Inf Tradit Chin Med, 2017, 5(24):82
[5] YBZ24102005-2015Z,国家食品药品监督管理局标准[S]. 2015YBZ24102005-2015Z, China Food and Drug Administration Standards[S]. 2015
[6] 中国药典2015版.一部 [S]. 2015:158ChP 2015.VolⅠ[S]. 2015:158
[7] 周海玲,许舜军,杨柳. HPLC法测定赤芍中芍药苷的含量[J]. 临床医学工程,2011,18(4):487ZHOU HL, XU SJ,YANG L. Determination of paeoniflorin in Radix Paeoniae Rubra by HPLC[J]. Clin Med Eng, 2011, 18(4):487
[8] 贾法强,尹宁宁,林林. HPLC法测定忍冬藤中绿原酸的含量[J]. 齐鲁药事,2007,1(26):26JIA FQ, YIN NN, LIN L. Determination of chlorogenic acid in Honeysuckle Stem by HPLC[J]. Qilu Pharm Aff, 2007,1(26):26