中国食源性金黄色葡萄球菌耐药及肠毒素特征
|
摘要
目的了解2017年中国食源性金黄色葡萄球菌(金葡菌)耐药和肠毒素特征。方法 2017年收集全国29个省市食源性金葡菌397株,并测定其抗生素耐药性、肠毒素基因和产肠毒素情况。结果 96.5%(383/397)金葡菌对受试的13种抗生素呈不同程度耐药,其中青霉素(PEN)耐药率最高(94.7%);30.2%(120/397)金葡菌为多重耐药,米面制品和面包来源金葡菌多重耐药率最高,分别为37.4%和37.3%;共存在72种耐药谱。284株金葡菌检出了20种肠毒素基因,其中sea、seb、sec和sed等常见肠毒素基因检出82株(20.7%,82/397),其中sea(14.9%)基因检出率明显高于seb～sed基因(χ~2=74.534,P <0.05),90%以上的菌株均能产生SE-A～SE-D肠毒素。结论 2017年中国29个省市食源性金葡菌耐药水平较高,多重耐药现象严重,且同时携带多种肠毒素基因并能产肠毒素。
Objective To examine antimicrobial resistance and enterotoxigenicity of foodborne Staphylococcus aureus(S. aureus) isolates in 29 provincial regions of China in 2017. Methods Totally, 397 foodborne S. aureus isolates were collected in 29 provincial regions of China in 2017 and antimicrobial susceptibility, enterotoxin genes, and toxigenic capability of all isolates were tested. Results In total, 96.5%(383/397) of the S. aureus isolates exhibited different resistant level to 13 antimicrobials tested. The resistance rate to penicillin(PEN) was the highest(94.7%). Moreover, 30.2%(120/397)were identified as multi-drug resistant(MDR) strains. Isolates from rice and flour products showed the most MDR, and the MDR rates were 7.4%(34/91) and 37.3%(19/51), respectively. A total of 72 drug resistant spectrums were identified from all397 isolates. Furthermore, 20 virulence genes were detected in 284 isolates. The typical SEs genes of sea, seb, sec and sed were detected in 82 strains(20.7%, 82/397) with sea(14.9%) and sec(5.0%) as the most frequently detected. More than 90%of the sea to sed carrying isolates were confirmed with the capacity to produce enterotoxins. Conclusion An overall high level antimicrobial resistance was found among foodborne S. aureus isolates in China in 2017, so was the MDR condition,especially for strains recovered from some foods. Virulence genes could be frequently detected in foodborne S. aureus isolates, most of which could produce enterotoxins.
Objective To examine antimicrobial resistance and enterotoxigenicity of foodborne Staphylococcus aureus(S. aureus) isolates in 29 provincial regions of China in 2017. Methods Totally, 397 foodborne S. aureus isolates were collected in 29 provincial regions of China in 2017 and antimicrobial susceptibility, enterotoxin genes, and toxigenic capability of all isolates were tested. Results In total, 96.5%(383/397) of the S. aureus isolates exhibited different resistant level to 13 antimicrobials tested. The resistance rate to penicillin(PEN) was the highest(94.7%). Moreover, 30.2%(120/397)were identified as multi-drug resistant(MDR) strains. Isolates from rice and flour products showed the most MDR, and the MDR rates were 7.4%(34/91) and 37.3%(19/51), respectively. A total of 72 drug resistant spectrums were identified from all397 isolates. Furthermore, 20 virulence genes were detected in 284 isolates. The typical SEs genes of sea, seb, sec and sed were detected in 82 strains(20.7%, 82/397) with sea(14.9%) and sec(5.0%) as the most frequently detected. More than 90%of the sea to sed carrying isolates were confirmed with the capacity to produce enterotoxins. Conclusion An overall high level antimicrobial resistance was found among foodborne S. aureus isolates in China in 2017, so was the MDR condition,especially for strains recovered from some foods. Virulence genes could be frequently detected in foodborne S. aureus isolates, most of which could produce enterotoxins.
引文
[1]Diep BA,Gill SR,Chang RF,et al.Complete genome sequence of USA300,an epidemic clone of community-acquired methicillinresistant Staphylococcus aureus[J].Lancet,2006,367(9512):731-739.
[2]Gharsa H,Ben SK,Lozano C,et al.Prevalence,antibiotic resistance,virulence traits and genetic lineages of Staphylococcus aureus in healthy sheep in Tunisia[J].Veterinary Microbiology,2012,156(3-4):367-373.
[3]Papadopoulos P,Papadopoulos T,Angelidis AS,et al.Prevalence of Staphylococcus aureus and of methicillin-resistant S.aureus(MRSA)along the production chain of dairy products in northwestern Greece[J].Food Microbiology,2018,69:43-50.
[4]Clinical and Laboratory Standards Institute(CLSI).Methods for antimicrobial disk and dilution susceptibility testing of infrequently isolated or fastidious bacteria;approved guideline[S].Wayne Pennsylvania:Clinical and Laboratory Standards Institute,2015.
[5]Vu BG,Stach CS,Salgado-Pabon W,et al.Superantigens of Staphylococcus aureus from patients with diabetic foot ulcers[J].Journal of Infectious Diseases,2014,210(12):1920-1927.
[6]容冬丽,吴清平,吴诗,等.我国部分地区即食食品和蔬菜中金黄色葡萄球菌污染分布及耐药和基因分型情况[J].微生物学报,2018,58(2):314-323.
[7]顾其芳,张红芝,刘玥,等.金黄色葡萄球菌食品分离株肠毒素基因及耐药性研究[J].中国卫生检验杂志,2018,28(11):1321-1323.
[8]张健,陈惠玲,邓志爱,等.广州市食源性金黄色葡萄球菌肠毒素及耐药分析[J].实用预防医学,2018,25(4):398-400.
[9]赵英芳,乔玫,宋晓红,等.金黄色葡萄球菌食品分离株与临床分离株耐药谱的相关性分析[J].中国药物与临床,2019,19(5):830-831.
[10]Govindaraj AV,Vanitha A.WHO global priority pathogens list on antibiotic resistance:an urgent need for action to integrate one health data[J].Perspect in Public Health,2018,138:87-88.
[11]Ge B,Mukherjee S,Hsu CH,et al.MRSA and multidrug-resistant Staphylococcus aureus in U.S.retail meats,2010-2011[J].Food Microbiology,2017,62:289-297.
[12]Tang Y,Larsen J,Kjeldgaard J,et al.Methicillin-resistant and susceptible Staphylococcus aureus from retail meat in Denmark[J].International Journal of Food Microbiology,2017,249:72-76.
[13]Zogg AL,Zurfluh K,Nüesch-Inderbinen M,et al.Characteristics of ESBL-producing Enterobacteriaceae and methicillin resistant Staphylococcus aureus(MRSA)isolated from Swiss and imported raw poultry meat collected at retail level[J].Schweiz Arch Tierheilkd,2016,158:451-456.
[14]Wang W,Baloch Z,Jiang T,et al.Enterotoxigenicity and antimicrobial resistance of Staphylococcus aureus isolated from retail food in China[J].Frontiers in Microbiology,2017,8:2256.
[15]Zhang J,Suo Y,Zhang D,et al.Genetic and virulent difference between pigmented and non-pigmented Staphylococcus aureus[J].Frontiers in Microbiology,2018,9:598.
[16]王园园,李颖,朱美娟,等.北京市顺义区食源性金黄色葡萄球菌肠毒素分型方法研究[J].首都公共卫生,2015,9(3):123-125.
[17]李光辉,郭卫芸,高雪丽,等.2003-2015年金黄色葡萄球菌食物中毒事件特征分析[J].食品研究与开发,2018,39(6):200-203.
[18]叶素贞,郑雯菱,黄火寿.食品中金黄色葡萄球菌污染状况及其肠毒素基因分析[J].海峡预防医学杂志,2016,22(1):60-61.
[19]张锋,顾敏霞,戴正,等.一起金黄色葡萄球菌肠毒素食物中毒事件调查[J].中国农村卫生事业管理,2017,37(7):803-805.
[20]耿雪峰,李薇薇,潘娜,等.中国2002-2016年单位食堂食物中毒情况分析[J].中国公共卫生,2018,34(6):858-860.
[21]于维森,王伟栋,孙建平.常见细菌性食物中毒快速处置[M].北京:人民卫生出版社,2013:36-37.
[2]Gharsa H,Ben SK,Lozano C,et al.Prevalence,antibiotic resistance,virulence traits and genetic lineages of Staphylococcus aureus in healthy sheep in Tunisia[J].Veterinary Microbiology,2012,156(3-4):367-373.
[3]Papadopoulos P,Papadopoulos T,Angelidis AS,et al.Prevalence of Staphylococcus aureus and of methicillin-resistant S.aureus(MRSA)along the production chain of dairy products in northwestern Greece[J].Food Microbiology,2018,69:43-50.
[4]Clinical and Laboratory Standards Institute(CLSI).Methods for antimicrobial disk and dilution susceptibility testing of infrequently isolated or fastidious bacteria;approved guideline[S].Wayne Pennsylvania:Clinical and Laboratory Standards Institute,2015.
[5]Vu BG,Stach CS,Salgado-Pabon W,et al.Superantigens of Staphylococcus aureus from patients with diabetic foot ulcers[J].Journal of Infectious Diseases,2014,210(12):1920-1927.
[6]容冬丽,吴清平,吴诗,等.我国部分地区即食食品和蔬菜中金黄色葡萄球菌污染分布及耐药和基因分型情况[J].微生物学报,2018,58(2):314-323.
[7]顾其芳,张红芝,刘玥,等.金黄色葡萄球菌食品分离株肠毒素基因及耐药性研究[J].中国卫生检验杂志,2018,28(11):1321-1323.
[8]张健,陈惠玲,邓志爱,等.广州市食源性金黄色葡萄球菌肠毒素及耐药分析[J].实用预防医学,2018,25(4):398-400.
[9]赵英芳,乔玫,宋晓红,等.金黄色葡萄球菌食品分离株与临床分离株耐药谱的相关性分析[J].中国药物与临床,2019,19(5):830-831.
[10]Govindaraj AV,Vanitha A.WHO global priority pathogens list on antibiotic resistance:an urgent need for action to integrate one health data[J].Perspect in Public Health,2018,138:87-88.
[11]Ge B,Mukherjee S,Hsu CH,et al.MRSA and multidrug-resistant Staphylococcus aureus in U.S.retail meats,2010-2011[J].Food Microbiology,2017,62:289-297.
[12]Tang Y,Larsen J,Kjeldgaard J,et al.Methicillin-resistant and susceptible Staphylococcus aureus from retail meat in Denmark[J].International Journal of Food Microbiology,2017,249:72-76.
[13]Zogg AL,Zurfluh K,Nüesch-Inderbinen M,et al.Characteristics of ESBL-producing Enterobacteriaceae and methicillin resistant Staphylococcus aureus(MRSA)isolated from Swiss and imported raw poultry meat collected at retail level[J].Schweiz Arch Tierheilkd,2016,158:451-456.
[14]Wang W,Baloch Z,Jiang T,et al.Enterotoxigenicity and antimicrobial resistance of Staphylococcus aureus isolated from retail food in China[J].Frontiers in Microbiology,2017,8:2256.
[15]Zhang J,Suo Y,Zhang D,et al.Genetic and virulent difference between pigmented and non-pigmented Staphylococcus aureus[J].Frontiers in Microbiology,2018,9:598.
[16]王园园,李颖,朱美娟,等.北京市顺义区食源性金黄色葡萄球菌肠毒素分型方法研究[J].首都公共卫生,2015,9(3):123-125.
[17]李光辉,郭卫芸,高雪丽,等.2003-2015年金黄色葡萄球菌食物中毒事件特征分析[J].食品研究与开发,2018,39(6):200-203.
[18]叶素贞,郑雯菱,黄火寿.食品中金黄色葡萄球菌污染状况及其肠毒素基因分析[J].海峡预防医学杂志,2016,22(1):60-61.
[19]张锋,顾敏霞,戴正,等.一起金黄色葡萄球菌肠毒素食物中毒事件调查[J].中国农村卫生事业管理,2017,37(7):803-805.
[20]耿雪峰,李薇薇,潘娜,等.中国2002-2016年单位食堂食物中毒情况分析[J].中国公共卫生,2018,34(6):858-860.
[21]于维森,王伟栋,孙建平.常见细菌性食物中毒快速处置[M].北京:人民卫生出版社,2013:36-37.