响应面法优化提取香菜根中总黄酮的工艺研究
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摘要
目的:采用响应面法优化香菜根中总黄酮的提取条件。方法:采用乙醇热浸提法,在提取时间、提取温度、液料比和乙醇体积分数这些单因素试验基础上,选取试验因素和水平,运用Design-Expert V8.0.6软件采用三因素三水平进行响应面分析,将香菜根中总黄酮的含量作为响应值。结果:通过实验得到乙醇热浸提香菜根中总黄酮的最优工艺是提取温度71℃、液料比31∶1(mL/g)、乙醇体积分数71%。结论:该条件下总黄酮的提取量为0.1691mg/g。
Objective:Response surface methodology is used to optimize the extraction conditions of total flavonoids from coriander roots.Methods:Ethanol heat extraction method is used to select test factors and levels based on single factor experiments such as extraction time,extraction temperature,liquid-solid ratio and ethanol concentration.The response surface analysis of three factors and three levels is used with Design-Expert V8.0.6.The flavonoid content in coriander roots as the response value is used in response surface analysis.Result:The optimum extraction process of total flavonoids in ethanol-extracted coriander roots is obtained by experiments:extraction temperature is 71℃,liquid-solid ratio is 31∶1(mL/g),and ethanol volume fraction is 71%.Conclusion:Under such conditions,the total flavonoids extraction amount is 0.1691 mg/g.
Objective:Response surface methodology is used to optimize the extraction conditions of total flavonoids from coriander roots.Methods:Ethanol heat extraction method is used to select test factors and levels based on single factor experiments such as extraction time,extraction temperature,liquid-solid ratio and ethanol concentration.The response surface analysis of three factors and three levels is used with Design-Expert V8.0.6.The flavonoid content in coriander roots as the response value is used in response surface analysis.Result:The optimum extraction process of total flavonoids in ethanol-extracted coriander roots is obtained by experiments:extraction temperature is 71℃,liquid-solid ratio is 31∶1(mL/g),and ethanol volume fraction is 71%.Conclusion:Under such conditions,the total flavonoids extraction amount is 0.1691 mg/g.
引文
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[3]刘玉明,李珂娴,何颖,等.NaNO2-Al(NO3)3-NaOH比色法测定枇杷花水提液中总黄酮含量[J].中华中医药学刊,2018(1):248-251.
[4]刘英英,朱宏斌.胡荽的传入及其本土化[J].农业考古,2014(6):244-248.
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[10]Woodman O L,Meeker W F,Boujaoude M.Vasorelaxant and antioxidant activity of flavonols and flavones:structureactivity relationships[J].J Cardiovasc Pharmacol,2005,46(3):302-309.
[11]李嵘,金美芳.微波法提取银杏黄酮甙的新工艺[J].食品科学,2000(2):39-40.
[12]孙智敏,李发堂,殷蓉,等.黄酮类化合物提取工艺研究进展[J].河北化工,2005(4):7-8.
[13]曹纬国,刘志勤,邵云,等.黄酮类化合物药理作用的研究进展[J].西北植物学报,2003,23(12):2241-2247.
[14]郭雪峰,岳永德.黄酮类化合物的提取,分离纯化和含量测定方法的研究进展[J].安徽农业科学,2007,35(26):8083-8086.
[15]Vanttinen K,Moravcova J.Phytoestrogen in soy foods:determination of daidzein and genistein by capillary electrophoreses[J].J Food Sci,1999,17(2):61-67.
[16]何洁,何芮,严奉伟.鱼腥草总黄酮的提取方法与抗氧化活性研究[J].食品科技,2014(3):198-201.
[17]张虹,王景李,白红丽,等.云南翠雀花根中总黄酮提取方法的研究[J].北方园艺,2014(13):153-154.
[18]梁鹏举,姜建辉,陈敏.响应面法优化巴旦木总黄酮提取工艺研究[J].食品研究与开发,2017,38(9):50-54.
[2]刘恒蔚,潘玉欣,谈知文.香菜黄酮的乙醇回流与超声波辅助提取工艺对比研究[J].湖北农业科学,2011,50(1):142-145.
[3]刘玉明,李珂娴,何颖,等.NaNO2-Al(NO3)3-NaOH比色法测定枇杷花水提液中总黄酮含量[J].中华中医药学刊,2018(1):248-251.
[4]刘英英,朱宏斌.胡荽的传入及其本土化[J].农业考古,2014(6):244-248.
[5]刘香荣,鲁海波,朱海泉,等.芫荽的研究进展[J].中国调味品,2012,37(6):17-19.
[6]陆占国,郭红转,李伟.芫荽根部芳香成分研究[J].化学与黏合,2007,29(2):79-81.
[7]朱海扬,曾慧兰.黄酮类化合物药理作用的研究进展[J].山东医药,2009,49(17):114-115.
[8]Samira L B,Khodir M.Phenolic contents and antioxidant activity of orange varieties(Citrus sinensis L.and Citrus aurantium L.)cultivated in Algeria:peels and leaves[J].Industrial Crops and Products,2013,50:723-730.
[9]Goya R G,Sarkar D K,Brown O A,et al.Potential of gene therapy for the treatment of pituitary tumors[J].Current Gene Therapy,2004,4(1):79-87.
[10]Woodman O L,Meeker W F,Boujaoude M.Vasorelaxant and antioxidant activity of flavonols and flavones:structureactivity relationships[J].J Cardiovasc Pharmacol,2005,46(3):302-309.
[11]李嵘,金美芳.微波法提取银杏黄酮甙的新工艺[J].食品科学,2000(2):39-40.
[12]孙智敏,李发堂,殷蓉,等.黄酮类化合物提取工艺研究进展[J].河北化工,2005(4):7-8.
[13]曹纬国,刘志勤,邵云,等.黄酮类化合物药理作用的研究进展[J].西北植物学报,2003,23(12):2241-2247.
[14]郭雪峰,岳永德.黄酮类化合物的提取,分离纯化和含量测定方法的研究进展[J].安徽农业科学,2007,35(26):8083-8086.
[15]Vanttinen K,Moravcova J.Phytoestrogen in soy foods:determination of daidzein and genistein by capillary electrophoreses[J].J Food Sci,1999,17(2):61-67.
[16]何洁,何芮,严奉伟.鱼腥草总黄酮的提取方法与抗氧化活性研究[J].食品科技,2014(3):198-201.
[17]张虹,王景李,白红丽,等.云南翠雀花根中总黄酮提取方法的研究[J].北方园艺,2014(13):153-154.
[18]梁鹏举,姜建辉,陈敏.响应面法优化巴旦木总黄酮提取工艺研究[J].食品研究与开发,2017,38(9):50-54.