封丘金银花炭疽病病原的分离和鉴定
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摘要
为明确河南封丘金银花炭疽病病原种类,对采自封丘的金银花炭疽病病叶,依据柯赫氏法则进行分离、纯化、回接和再分离,证明获得的分离菌株是金银花炭疽病的致病菌.对其培养性状、在寄主上的形态特征及萌发方式进行观察,并对其r DNA-ITS序列进行分析,结果表明:致病菌株在PDA培养基上菌落呈白色,后期菌落中央颜色变暗,生分生孢子盘;分生孢子盘具黑色刚毛,分生孢子长圆柱形,两端钝圆,单细胞.核糖体DNA-ITS序列与GenBank数据库胶孢炭疽菌同源性达99%.将封丘金银花炭疽病病原菌鉴定为胶孢炭疽菌Colletotrichum gloeosporioides.
In order to determine the pathogen of Lonicera japonica anthracnose in Henan Province,infected leaves were collected from Fengqiu,Henan Province.The pathogen was isolated,back-inoculated,re-isolated by Koch's postulates and was identified according to morphological and culture characteristics and the sequence of ribosomal DNA-ITS.The results showed that the fungus colonies on PDA were white,later pale yellow.Acervuli with setae formed in the center of the colonies.Conidia long cylindrical,obtuse at both ends, single-celled.rDNA-ITS sequence analysis indicated that the pathogen and Colletotrichum gloeosporioidesisolate COL4 were 99% in homology. he pathogen was identified as Colletotrichum gloeosporioides.
In order to determine the pathogen of Lonicera japonica anthracnose in Henan Province,infected leaves were collected from Fengqiu,Henan Province.The pathogen was isolated,back-inoculated,re-isolated by Koch's postulates and was identified according to morphological and culture characteristics and the sequence of ribosomal DNA-ITS.The results showed that the fungus colonies on PDA were white,later pale yellow.Acervuli with setae formed in the center of the colonies.Conidia long cylindrical,obtuse at both ends, single-celled.rDNA-ITS sequence analysis indicated that the pathogen and Colletotrichum gloeosporioidesisolate COL4 were 99% in homology. he pathogen was identified as Colletotrichum gloeosporioides.
引文
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[16] MORRALL R A.Canadian Plant Disease Survey[R].CPDS,2013,93:1-188.
[17]杨友联,李树江,胡海雪,等.灰毡毛忍冬顶枯病病原菌的分子鉴定[J].西南农业学报,2015,28(5):2107-2111.
[2]房海灵,于盱,亓希武,等.金银花种质资源及育种研究进展[J].江西农业学报,2017,29(9):45-50.
[3] WEIR B S,JOHNSTON P R,DAMM U.The Colletotrichum gloeosporioides species complex[J].Studies in Mycology,2012,73:115-180.
[4] SUTTON B C.The coelomycetes-fungi imperfect with Pycnidia,Acervuli and Stromata[J].Commonwealth,1980,3:523-538.
[5] ELFAR K,TORRES R,DIAZ G A,et al.Characterization of Diaporthe australafricana and Diaporthe spp. associated with stem canker of blueberry in Chile[J].Plant Disease,2013,97(8):1042-1050.
[6] CANNON P F,BUDDIE A G,BRIDGE P D.The Typification of Colletotrichum gloeosporioides[J].Mycotaxon,2008,104:189-204.
[7]王广军,杨建丽,高国华.金银花病虫害综合防治技术[J].河南农业科学,2003(9):69-70.
[8]吴朝峰,马雪梅.河南金银花病虫害防治现状及对策[J].湖北农业科学,2012,51(13):2732-2735.
[9]李正茂,夏永刚,周刚,等.湖南金银花病虫害防治现状及对策[J].中国农村小康科技,2010(12):66-68.
[10]李玉奎,胡万银,张艳红,等.金银花病虫害生态防控技术[J].河北果树,2017(2):37-38.
[11]简美玲,郑基焕,毛润乾.广东省金银花主要病虫害调查初报[J].广东农业科学,2011,38(14):74-76.
[12]付嵘.金银花炭疽病的初步研究[D].雅安:四川农业大学,2012.
[13]胡中常,覃晓春,范祝元,等.隆回灰毡毛忍冬(大叶金银花)病虫害调查初报[J].湖南林业科技,2005(1):47-48.
[14] WAIPARA N W,WINKS C J,SMITH L A,et al.Invertebrates and Fungi Associated with Japanese Honeysuckle,Lonicera japonica(Caprifoliaceae),in New Zealand[R].Landcare research contract report:LC0506/026,2005.
[15] WAIPARA N W,WINKS C J,SMITH L A,et al.Natural enemies of Japanese honeysuckle,Lonicera japonica,in New Zealand[J].New Zealand Plant Protection,2007,60:158-163.
[16] MORRALL R A.Canadian Plant Disease Survey[R].CPDS,2013,93:1-188.
[17]杨友联,李树江,胡海雪,等.灰毡毛忍冬顶枯病病原菌的分子鉴定[J].西南农业学报,2015,28(5):2107-2111.