四氯化碳致小鼠急性肝损伤模型造模要素及中医药防治的数据挖掘研究
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摘要
目的:研究四氯化碳(CCL4)致小鼠肝损伤动物模型造模要素,为提高造模效率及评价药物有效性提供方法学参考。方法:收集1990-2016年期间研究CCL_4致小鼠肝损伤动物模型的393篇文献,录入动物种别、体重、性别,CCL_4的稀释液种类、稀释液浓度、给予体积和剂量,测试指标、治疗药物等数据。结果:动物常用20±2 g的雄性小鼠;CCL_4的稀释液最常使用花生油,浓度多为0.1%和0.2%,给予体积多为10 mg﹒kg~(-1),给予剂量多为0.01 mg﹒kg~(-1),多采用腹腔注射。常检测指标有谷丙转氨酶、谷草转氨酶、丙二醛、超氧化物歧化酶等。阳性对照药物主要有联苯双酯、水飞蓟素、甘利欣等。常用中药材有黄芪、甘草、丹参、茵陈、柴胡、黄芩、五味子、栀子、白花蛇舌草、决明子、大青叶等。结论:CCL_4致小鼠肝损伤动物模型造模时,建议选择20±2 g的雄性小鼠,联苯双酯做阳性对照组,预防给药14天,末次给药1 h后腹腔注射10 mg﹒kg~(-1)的浓度为0.1%的CCL_4花生油溶液,于造模后24 h处死,取血测试生化指标,取肝脏做组织病理学研究。
Objective: To study the modeling factors of CCL_4 induced liver damage in mice, and provide methodology references for improving model efficiency and evaluating drug effectiveness. Method: A total of 393 documents researching CCL_4-induced liver injury in mice models during 1990-2016 were collected and the related data were recorded including animal species, body weight, gender, CCL_4 diluent, diluent concentration, drug volume and dose, test indicators, and treatment drugs etc. Result: Male mice(20±2 g) were commonly used as experimental animals. Peanut oil was frequently adopted for CCL_4 diluent with the solution concentration of 0.1% or 0.2% in most cases. The CCL_4 volume was mainly 10 mg﹒kg~(-1) with the dosage of 0.01 mg﹒kg~(-1). Abdominal injection was used in most cases. Tested indexes included glutamic-pyruvic transaminase, glutamic oxalacetic transaminase, malondialdehyde and superoxide dismutase, etc. The positive control drugs mainly included bifendate, silymarin, and diammonium glycyrrhizinate etc. Commonly used Chinese medicine included Huangqi, Gancao, Danshen, Yinchen, Chaihu, Huangqin, Wuweizi, Zhizi, Baihuasheshecao, Juemingzi, and Daqingye etc. Conclusion: It is suggested that 20±2 g male mice can be selected for CCL_4 induced liver injury in mice model with bifendate as the positive control group. Drug was given for prevention treatment for 14 days, and 10 mg﹒kg~(-1) CCL_4 in 0.1% peanut oil should be injected one hour after the last injection. 24 h after model establishment, the mice is sacrificed to test its blood for biochemical indexes and its liver is taken for histopathologic study.
Objective: To study the modeling factors of CCL_4 induced liver damage in mice, and provide methodology references for improving model efficiency and evaluating drug effectiveness. Method: A total of 393 documents researching CCL_4-induced liver injury in mice models during 1990-2016 were collected and the related data were recorded including animal species, body weight, gender, CCL_4 diluent, diluent concentration, drug volume and dose, test indicators, and treatment drugs etc. Result: Male mice(20±2 g) were commonly used as experimental animals. Peanut oil was frequently adopted for CCL_4 diluent with the solution concentration of 0.1% or 0.2% in most cases. The CCL_4 volume was mainly 10 mg﹒kg~(-1) with the dosage of 0.01 mg﹒kg~(-1). Abdominal injection was used in most cases. Tested indexes included glutamic-pyruvic transaminase, glutamic oxalacetic transaminase, malondialdehyde and superoxide dismutase, etc. The positive control drugs mainly included bifendate, silymarin, and diammonium glycyrrhizinate etc. Commonly used Chinese medicine included Huangqi, Gancao, Danshen, Yinchen, Chaihu, Huangqin, Wuweizi, Zhizi, Baihuasheshecao, Juemingzi, and Daqingye etc. Conclusion: It is suggested that 20±2 g male mice can be selected for CCL_4 induced liver injury in mice model with bifendate as the positive control group. Drug was given for prevention treatment for 14 days, and 10 mg﹒kg~(-1) CCL_4 in 0.1% peanut oil should be injected one hour after the last injection. 24 h after model establishment, the mice is sacrificed to test its blood for biochemical indexes and its liver is taken for histopathologic study.
引文
[1]陈奇.中药药理研究方法学[M].北京:人民卫生出版社2011:818.
[2]李仪奎.中药药理实验方法学[M].上海:上海科学技术出版社2006:514.
[3]陈雪龙,齐艳萍.四氯化碳急性肝损伤小鼠血清及肝脏组织学变化[J].中国兽医杂志,2011,47(1):39.
[4]那玉清,保守魁.八味獐芽菜散对小鼠急性肝损伤的保护作用[J].青海医学院学报,2011,32(4):253.
[5]姜汹,李家奎.藏药波棱瓜子提取液对四氯化碳致小鼠急性肝损伤保护机理的研究[J].湖北农业科学,2014,53(4):856.
[6]陈欢,林芳,杨振华,等.水飞蓟宾·熊去氧胆酸蒸发共沉淀物对CCL4致小鼠急性肝损伤的保护作用[J].中国应用生理学杂志,2016,32(1):38.
[7]朱安妮,李蕊,刘三海,等.四氯化碳诱导小鼠急性肝损伤模型的建立和优化[J].中国肝脏病杂志(电子版),2014,6(1):27.
[8]谢湘媚,刘志伟,林少宾,等.四氯化碳致小鼠急性肝损伤的性别差异及其与雄激素受体表达的关系[J].解剖学研究,2007,29(6):417.
[9]娄猛猛,李国玉,王航宇,等.四氯化碳致小鼠急性肝损伤模型的研究[J].农垦医学,2010,32(3):196.
[10]贾俊清,贾俊英.水飞蓟宾一磷脂酰胆碱复合物对小鼠急性肝损伤的预防和治疗作用[J].广西医科大学学报,2006,23(4):605.
[11]尹雪雁,伊辛,许凉凉,等冰飞蓟宾微粒分散体对CCL4所致小鼠急性肝损伤的预防作用研究[J].世界中医药,2016,11(3):490.
[12]肖东征,高苋,马宁水飞蓟素磷脂复合物对化学性肝损伤的保护作用[J].中国自然医学杂志,2005,7(3):205.
[13]许红霞,高晓黎.水飞蓟素脂质体对小鼠CCL4急性肝损伤保护作用的实验研究[J].新疆医科大学学报,2016,29(5):421.
[14]苏治合,温飞,蔡栩,等.四黄汤对急性肝损伤小鼠保护作用的研究[J].大理学院学报,2011,10(12):44.
[15]洪性勋,尹明浩.朝医二门五味汤对四氯化碳引起小鼠急性肝损伤的保护作用[J].中国民族医药杂志.2010,5(5):39.
[16]金俊杰,钟鸣,余胜民,等.穿破石对四氯化碳所致小鼠急性肝损伤的保护作用[J].时珍国医国药,2012,23(8):1903.
[17]尹学哲,赵文玺,金爱花,等.大豆异黄酮对四氯化碳致小鼠肝脏氧化应激和DNA损伤的干预作用[J].食品科学,2014,35(1):214.
[18]蒋真真,袁带秀,胡倩,等.杜仲总黄酮对小鼠急性肝损伤的保护作用[J].广州化工,2016,44(2):69.
[19]王刚.红景天总黄酮对小鼠急性肝损伤保护作用研究[J].实用中医药杂志,2016,32(6):518.
[20]鲍琛.虎杖提取物对CCL4诱导的小鼠急性肝损伤的保护作用[J].海峡药学,2010,22(6):36.
[21]鲍红月,陈子越,杨波.虎杖提取物对小鼠急性肝损伤的保护作用[J].海峡药学,2011,23(7):40.
[22]张光海,王盟,刘亚楠.黄精提取物对急性肝损伤小鼠的保护作用[J].医药导报,201,32(5):593.
[23]刘文艺,吴鸿.黄芪丹参合剂对小鼠CC14急性肝损伤保护作用的实验研究[J].中国实验诊断学,2011,15(8):1293.
[24]宁康健,吕锦芳,姜锦鹏,等.黄芩苷对小鼠四氯化碳肝损伤保护作用的研究[J].中国中医药科技,2011,18(2):120.
[25]韩泳,王玉,崔燕芒,等.黄芩素对四氯化碳诱导小鼠急性肝损伤的保护作用[J].中国药理学通报,2013,29(6):883.
[26]陈红莲.金银花对四氯化碳所致小鼠急性肝损伤的影响[J].中国老年学杂志,2011,31(16):3086.
[27]康宏杰,张霞一,侯延辉,等.秦艽对四氯化碳致小鼠急性肝损伤的保护作用[J].中药药理与临床,2012,28(6):98.
[28]唐晓光.青藏龙胆对四氯化碳所致小鼠急性肝损伤的保护作用[J].赤峰学院学报(自然科学版),2011. 27(3):147.
[29]马永贵,罗桂花,陈志,等.小鼠四氯化碳急性肝损伤模型稳定性初探[J].青海师范大学学报(自然科学版),2006,1:88.
[2]李仪奎.中药药理实验方法学[M].上海:上海科学技术出版社2006:514.
[3]陈雪龙,齐艳萍.四氯化碳急性肝损伤小鼠血清及肝脏组织学变化[J].中国兽医杂志,2011,47(1):39.
[4]那玉清,保守魁.八味獐芽菜散对小鼠急性肝损伤的保护作用[J].青海医学院学报,2011,32(4):253.
[5]姜汹,李家奎.藏药波棱瓜子提取液对四氯化碳致小鼠急性肝损伤保护机理的研究[J].湖北农业科学,2014,53(4):856.
[6]陈欢,林芳,杨振华,等.水飞蓟宾·熊去氧胆酸蒸发共沉淀物对CCL4致小鼠急性肝损伤的保护作用[J].中国应用生理学杂志,2016,32(1):38.
[7]朱安妮,李蕊,刘三海,等.四氯化碳诱导小鼠急性肝损伤模型的建立和优化[J].中国肝脏病杂志(电子版),2014,6(1):27.
[8]谢湘媚,刘志伟,林少宾,等.四氯化碳致小鼠急性肝损伤的性别差异及其与雄激素受体表达的关系[J].解剖学研究,2007,29(6):417.
[9]娄猛猛,李国玉,王航宇,等.四氯化碳致小鼠急性肝损伤模型的研究[J].农垦医学,2010,32(3):196.
[10]贾俊清,贾俊英.水飞蓟宾一磷脂酰胆碱复合物对小鼠急性肝损伤的预防和治疗作用[J].广西医科大学学报,2006,23(4):605.
[11]尹雪雁,伊辛,许凉凉,等冰飞蓟宾微粒分散体对CCL4所致小鼠急性肝损伤的预防作用研究[J].世界中医药,2016,11(3):490.
[12]肖东征,高苋,马宁水飞蓟素磷脂复合物对化学性肝损伤的保护作用[J].中国自然医学杂志,2005,7(3):205.
[13]许红霞,高晓黎.水飞蓟素脂质体对小鼠CCL4急性肝损伤保护作用的实验研究[J].新疆医科大学学报,2016,29(5):421.
[14]苏治合,温飞,蔡栩,等.四黄汤对急性肝损伤小鼠保护作用的研究[J].大理学院学报,2011,10(12):44.
[15]洪性勋,尹明浩.朝医二门五味汤对四氯化碳引起小鼠急性肝损伤的保护作用[J].中国民族医药杂志.2010,5(5):39.
[16]金俊杰,钟鸣,余胜民,等.穿破石对四氯化碳所致小鼠急性肝损伤的保护作用[J].时珍国医国药,2012,23(8):1903.
[17]尹学哲,赵文玺,金爱花,等.大豆异黄酮对四氯化碳致小鼠肝脏氧化应激和DNA损伤的干预作用[J].食品科学,2014,35(1):214.
[18]蒋真真,袁带秀,胡倩,等.杜仲总黄酮对小鼠急性肝损伤的保护作用[J].广州化工,2016,44(2):69.
[19]王刚.红景天总黄酮对小鼠急性肝损伤保护作用研究[J].实用中医药杂志,2016,32(6):518.
[20]鲍琛.虎杖提取物对CCL4诱导的小鼠急性肝损伤的保护作用[J].海峡药学,2010,22(6):36.
[21]鲍红月,陈子越,杨波.虎杖提取物对小鼠急性肝损伤的保护作用[J].海峡药学,2011,23(7):40.
[22]张光海,王盟,刘亚楠.黄精提取物对急性肝损伤小鼠的保护作用[J].医药导报,201,32(5):593.
[23]刘文艺,吴鸿.黄芪丹参合剂对小鼠CC14急性肝损伤保护作用的实验研究[J].中国实验诊断学,2011,15(8):1293.
[24]宁康健,吕锦芳,姜锦鹏,等.黄芩苷对小鼠四氯化碳肝损伤保护作用的研究[J].中国中医药科技,2011,18(2):120.
[25]韩泳,王玉,崔燕芒,等.黄芩素对四氯化碳诱导小鼠急性肝损伤的保护作用[J].中国药理学通报,2013,29(6):883.
[26]陈红莲.金银花对四氯化碳所致小鼠急性肝损伤的影响[J].中国老年学杂志,2011,31(16):3086.
[27]康宏杰,张霞一,侯延辉,等.秦艽对四氯化碳致小鼠急性肝损伤的保护作用[J].中药药理与临床,2012,28(6):98.
[28]唐晓光.青藏龙胆对四氯化碳所致小鼠急性肝损伤的保护作用[J].赤峰学院学报(自然科学版),2011. 27(3):147.
[29]马永贵,罗桂花,陈志,等.小鼠四氯化碳急性肝损伤模型稳定性初探[J].青海师范大学学报(自然科学版),2006,1:88.