单抗制备过程中对小鼠的三种免疫方法的比较研究
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摘要
本实验对单抗制备过程中小鼠的三种免疫方法进行比较,确定三种免疫方法对小鼠免疫的免疫效价、操作难易程度、耗费时间和免疫成本等关键参数,同时指出肌肉注射法在免疫效价方面的缺陷、静脉注射法在操作方面的缺陷,建议采用经济省时、简便易行、免疫效价高的皮下多点注射结合腹腔注射法作为小鼠免疫方法的优先选择,应用于单抗的制备。
In this experiment, three immunization methods of mice in the preparation of monoclonal antibody were compared, and the key parameters such as immune potency, operation difficulty, time-consuming and immune cost of the three immunization methods were determined. At the same time, the shortcomings of intramuscular injection method in immune potency and intravenous injection method in operation were pointed out. It is suggested that the economical, time-saving, simple and feasible subcutaneous multi-point injection combined with intraperitoneal injection, which is a preferred method for the preparation of monoclonal antibodies in mice.
In this experiment, three immunization methods of mice in the preparation of monoclonal antibody were compared, and the key parameters such as immune potency, operation difficulty, time-consuming and immune cost of the three immunization methods were determined. At the same time, the shortcomings of intramuscular injection method in immune potency and intravenous injection method in operation were pointed out. It is suggested that the economical, time-saving, simple and feasible subcutaneous multi-point injection combined with intraperitoneal injection, which is a preferred method for the preparation of monoclonal antibodies in mice.
引文
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[3]许道艳,刘磊,刘仁沿,等.麻痹性贝毒单克隆抗体的制备和酶联免疫检测方法的建立[J].中国免疫学杂志,2013,29(1):69-73.
[4]王云龙,翟晋豫,王继创,等.糖类抗原19-9单抗制备及DAS-ELISA的建立[J].中国免疫学杂志,2014,30(8):1088-1092.
[5]姚彩霞,李泸霞.脾内免疫法制备抗人IgM单克隆抗体的研究[J].细胞与分子免疫学杂志,1997,13(2):53.
[6]王玉亮,黄繁墙,穆红,等.脾内免疫法制备抗α2-抗纤溶酶单克隆抗体[J].中国免疫学杂志,2000,16(2):79.
[7]张红绪,胡萍,胡庚熙,等.抗Derp2单克隆抗体细胞株的建立与鉴定[J].河南师范大学学报(自然科学版),2008,36(4):136-139.
[8]温晓艳,周芳,张彦霞,等.抗流感嗜血杆菌外膜蛋白P6单克隆抗体的制备及鉴定[J].细胞与分子免疫学杂志,2014,30(10):1051-1053,1061.