腐胺增强香蕉果实低温耐受性的作用机制
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摘要
为了研究腐胺在采后香蕉果实低温耐受性中的作用及其作用机制,通过外源施加的方式,研究腐胺对采后香蕉果实的低温耐受表型、冷害指数、细胞膜完整性、膜脂过氧化程度、脂肪氧化酶活性及内源一氧化氮含量的影响。结果表明,低浓度的腐胺能够增强采后香蕉果实的低温耐受性,其中以5 mmol/L的效果最好。5 mmol/L的腐胺显著降低了7℃环境下香蕉的冷害指数和膜脂过氧化程度,有效维持细胞膜的完整性,而一氧化氮专一性清除剂抑制了腐胺对冷害指数的降低。初步研究其作用机制为:抑制了由低温导致的脂肪氧化酶活性的升高,表明信号分子一氧化氮在腐胺增强采后香蕉果实的低温耐受性过程中发挥关键作用。
To study function and mechanism of putrescine(Put) on chilling tolerance of postharvest banana fruit, the effects of exogenous Put on chilling tolerance phenotype, chilling injury index, integrity of cell membrane, membrane lipid peroxidation, lipoxygenase activity and endogenous nitric oxide content were investigated. The result showed that Put at low concentration induced chilling tolerance of postharvest banana fruit significantly and the optimal concentration is 5 mmol/L. Put of 5 mmol/L decreased chilling injury index, membrane lipid peroxidation and maintained integrity of cell membrane dramatically.Moreover, nitric oxide is involved in the process of Put-induced chilling tolerance of postharvest banana fruit because cPTIO, a nitric oxide scavenger, inversed the effect of Put. The mechanism is that Put inhibited chilling-induced lipoxygenase activity, showing exogenous Put increased chilling tolerance of postharvest banana fruit by inducing endogenous content of nitric oxide.
To study function and mechanism of putrescine(Put) on chilling tolerance of postharvest banana fruit, the effects of exogenous Put on chilling tolerance phenotype, chilling injury index, integrity of cell membrane, membrane lipid peroxidation, lipoxygenase activity and endogenous nitric oxide content were investigated. The result showed that Put at low concentration induced chilling tolerance of postharvest banana fruit significantly and the optimal concentration is 5 mmol/L. Put of 5 mmol/L decreased chilling injury index, membrane lipid peroxidation and maintained integrity of cell membrane dramatically.Moreover, nitric oxide is involved in the process of Put-induced chilling tolerance of postharvest banana fruit because cPTIO, a nitric oxide scavenger, inversed the effect of Put. The mechanism is that Put inhibited chilling-induced lipoxygenase activity, showing exogenous Put increased chilling tolerance of postharvest banana fruit by inducing endogenous content of nitric oxide.
引文
[1]Alcázar R,Cuevas JC,Planas J.Integration of polyamines in the cold acclimation response[J].Plant Science,2011,180:31-38.
[2]王勇,陆旺金,张昭其,等.ABA和腐胺处理减轻香蕉果实贮藏冷害[J].植物生理与分子生物学学报,2003,29(6):549-554.
[3]Zhang X,Shen L,Li F,et al.Methyl salicylate-induced arginine catabolism is associated with up-regulation of polyamine and nitric oxide levels and improves chilling tolerance in cherry tomato fruit[J].Journal Agricultural Food Chemistry,2011,59:9351-9357.
[4]Luo Z,Chen C,Xie J.Effect of salicylic acid treatment on alleviateng postharvest chilling injury of‘Qingnai’plum fruit[J].Postharvest Biology Technology,2011,62:115-120.
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[8]Wang YS,Luo ZS,Du R,et al.Effect of nitric oxide on antioxidative response and proline metabolism in banana during cold storage[J].J Agric Food Chemistry,2013,61(37):8880-8887.
[9]Wu B,Guo Q,Li Q,et al.Impact of postharvest nitric oxide treatment on antioxidant enzymes and related genes in banana fruit in response to chilling tolerance[J].Postharvest Biology Technology,2014,92:157-163.
[10]Nguyen TBT,Ketsa S,van Doorn WG.Relationship between browning and the activities of polyphenol oxidase and phenylalanine ammonia lyase in banana peel during low temperature storage[J].Postharvest Biology Technology,2003,30:187-193
[11]Wang Y,Luo Z,Huang X,et al.Effect of exogenousγ-aminobutyric acid(GABA)treatment on chilling injury and antioxidant capacity in banana peel[J].Scientia Horticulturae,2014,168:132-137.
[12]He Q,Hong K,Zou R,et al.The role of jasmonic acid and lipoxygenase in propylene-induced chilling tolerance on banana fruit[J].European Food Research Technology,2014,238:71-78.
[13]Groppa MD,Benavides MP.Polyamines and abiotic stress:recent advances[J].Amino Acids,2008,34:35-45.
[14]Takeda Y,Yoza K-I,Noga Y,et al.Putrescine accumulation in banana fruit with ripening during storage[J].Phytochemistry,1997,46:57-60.
[15]Valero D,Martínez-Romero,Serrano M.The role of polyamines in the improvement of the shelf life of fruit[J].Trends Food Science Technology,2002,13:228-234.
[16]Harindra Champa WA,Gill MIS,Mahajan BVC,et al.Postharvest treatment of polyamines maintains quality and extends shelf-life of table grapes(Vitis vinifera L.)cv.Flame Seedless[J].Postharvest Biology Technology,2014,91:57-63.
[17]Sirikesorn L,Imsabai W,Ketsa S,et al.Ethylene-induced water soaking in Dendrobium floral buds,accompanied by increased lipoxygenase and phospholipase D(PLD)activity and expression of a PLD gene[J].Postharvest Biology Technology,2015,108:48-53.
[18]Tun NN,Santa-Catarina C,Begum T,et al.Polyamines induce rapid biosynthesis of nitric oxide(NO)in Arabidopsis thaliana seedlings[J].Plant Cell Physiology,2006,47:346-354.
[19]Silveira V,santa-catarina C,Tun NN,et al.Polyamine effects on the endogenous polyamine contents,nitric oxide release,growth and differentiation of embryogenic suspension cultures of Araucaria angustifolia(Bert)[J].Plant Science,2006,171:91-98.
[20]Yang B,Zhang J,Wu Fe,et al.Polyamine-induced nitric oxide generation and its potential requirement for peroxide in suspension cells of soybean cotyledon node callus[J].Plant Physiology Biochemistry,2014,79:41-47.
[2]王勇,陆旺金,张昭其,等.ABA和腐胺处理减轻香蕉果实贮藏冷害[J].植物生理与分子生物学学报,2003,29(6):549-554.
[3]Zhang X,Shen L,Li F,et al.Methyl salicylate-induced arginine catabolism is associated with up-regulation of polyamine and nitric oxide levels and improves chilling tolerance in cherry tomato fruit[J].Journal Agricultural Food Chemistry,2011,59:9351-9357.
[4]Luo Z,Chen C,Xie J.Effect of salicylic acid treatment on alleviateng postharvest chilling injury of‘Qingnai’plum fruit[J].Postharvest Biology Technology,2011,62:115-120.
[5]Serrano M,Martínez-Madrid MC,Pretel MT,et al.Modified atmosphere packaging minimizes increases in putrescine and abscisic acid levels caused by chilling injury in pepper fruit[J].Journal Agricultural Food Chemistry,1997,45:1668-1672.
[6]Rodríguez SC,López B,Chaves AR.Effect of different treatments on the evolution of polyamines during refrigerated storage of eggplants[J].Journal Agricultural Food Chemistry,2001,49:4700-4705.
[7]Zhu XF,Wang B,Zheng SJ,et al.Putrescine alleviates Fe deficiency via NO-dependent reutilization of root cell wall Fe in Arabidopsis thaliana[J].Plant Physiology,2016,170(1):558-567.
[8]Wang YS,Luo ZS,Du R,et al.Effect of nitric oxide on antioxidative response and proline metabolism in banana during cold storage[J].J Agric Food Chemistry,2013,61(37):8880-8887.
[9]Wu B,Guo Q,Li Q,et al.Impact of postharvest nitric oxide treatment on antioxidant enzymes and related genes in banana fruit in response to chilling tolerance[J].Postharvest Biology Technology,2014,92:157-163.
[10]Nguyen TBT,Ketsa S,van Doorn WG.Relationship between browning and the activities of polyphenol oxidase and phenylalanine ammonia lyase in banana peel during low temperature storage[J].Postharvest Biology Technology,2003,30:187-193
[11]Wang Y,Luo Z,Huang X,et al.Effect of exogenousγ-aminobutyric acid(GABA)treatment on chilling injury and antioxidant capacity in banana peel[J].Scientia Horticulturae,2014,168:132-137.
[12]He Q,Hong K,Zou R,et al.The role of jasmonic acid and lipoxygenase in propylene-induced chilling tolerance on banana fruit[J].European Food Research Technology,2014,238:71-78.
[13]Groppa MD,Benavides MP.Polyamines and abiotic stress:recent advances[J].Amino Acids,2008,34:35-45.
[14]Takeda Y,Yoza K-I,Noga Y,et al.Putrescine accumulation in banana fruit with ripening during storage[J].Phytochemistry,1997,46:57-60.
[15]Valero D,Martínez-Romero,Serrano M.The role of polyamines in the improvement of the shelf life of fruit[J].Trends Food Science Technology,2002,13:228-234.
[16]Harindra Champa WA,Gill MIS,Mahajan BVC,et al.Postharvest treatment of polyamines maintains quality and extends shelf-life of table grapes(Vitis vinifera L.)cv.Flame Seedless[J].Postharvest Biology Technology,2014,91:57-63.
[17]Sirikesorn L,Imsabai W,Ketsa S,et al.Ethylene-induced water soaking in Dendrobium floral buds,accompanied by increased lipoxygenase and phospholipase D(PLD)activity and expression of a PLD gene[J].Postharvest Biology Technology,2015,108:48-53.
[18]Tun NN,Santa-Catarina C,Begum T,et al.Polyamines induce rapid biosynthesis of nitric oxide(NO)in Arabidopsis thaliana seedlings[J].Plant Cell Physiology,2006,47:346-354.
[19]Silveira V,santa-catarina C,Tun NN,et al.Polyamine effects on the endogenous polyamine contents,nitric oxide release,growth and differentiation of embryogenic suspension cultures of Araucaria angustifolia(Bert)[J].Plant Science,2006,171:91-98.
[20]Yang B,Zhang J,Wu Fe,et al.Polyamine-induced nitric oxide generation and its potential requirement for peroxide in suspension cells of soybean cotyledon node callus[J].Plant Physiology Biochemistry,2014,79:41-47.