日粮添加油脂和出生类型对羔羊肌肉胶原蛋白特性及相关基因表达的影响
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摘要
旨在研究日粮添加油脂和不同出生类型对羔羊肌肉胶原蛋白特性及相关基因表达的影响。本试验选取64只2月龄断奶双羔(twin born,TW;32只)和三羔(triplet born,TR;32只)湖羊公羔,分别饲喂基础日粮(control, CON)和添加2%大豆油(soybean oil,SBO)的日粮,采用2×2析因试验设计,分为4个处理(TW-CON,TW-SBO,TR-CON,TR-SBO),每个处理16个重复,每个重复1只羊。90 d后屠宰采集羔羊背最长肌,测定其胶原蛋白含量、相关酶活以及肌肉发育相关基因表达水平等。结果表明:1) TW组的Ⅰ型胶原蛋白含量显著高于TR组(P<0.05)。日粮添加油脂有提高可溶性胶原蛋白含量的趋势(0.050.05)。2)基质金属蛋白酶13(MMP-13)含量因日粮中油脂添加而显著下降(P<0.05),而吡啶诺林(PYD)和基质金属蛋白酶抑制剂(TIMP-1)则受日粮油脂添加和出生类型交互作用的显著影响(P<0.05)。3)MYOD1在双羔中表达水平显著高于三羔(P<0.05)。日粮油脂添加显著降低了FN1的相对表达水平(P<0.05),而FGF2和COL3A1的相对表达水平则表现出受日粮油脂添加和出生类型交互作用影响的趋势(0.050.05)。结果提示,双羔肌肉中胶原蛋白含量高于三羔,油脂有促进胶原蛋白含量增加的趋势,且日粮油脂和出生类型共同作用胶原蛋白代谢相关酶含量及基因的表达,进而影响肌肉中胶原蛋白的含量。
This study aimed to investigate the effects of dietary soybean oil supplementation and different birth types on collagen characteristics and related genes expression in muscle of lamb. Sixty-four male Hu lambs with twin born(TW, 32 lambs) and triplet born(TR, 32 lambs) types weaned at 2 months old were selected and fed basal diet(CON) or diet with 2% soybean oil supplemented(SBO). Lambs were randomly divided into 4 treatments(TW-CON, TW-SBO, TR-CON, TR-SBO) according to 2×2 factorial experimental design, each treatment had 16 biological replication, and 1 lamb in each replication. Lambs were slaughtered after 90 days of feeding, and longissimus dorsi muscle samples were collected for detecting the content of collagen, enzymes activity and muscle development-related genes expression level. The results indicated that: 1) The collagen type Ⅰ content in TW groups was significantly higher than that in TR groups(P<0.05). Dietary soybean oil supplementation increased collagen content(0.050.05). 2) Matrix metalloproteinases-13(MMP-13) content decreased significantly with diet soybean oil supplementation(P<0.05), while pyridinoline(PYD) and tissue inhibitor of metalloproteinases(TIMP-1) were affected significantly by the interaction effects between dietary soybean oil supplementation and birth types(P<0.05). 3) MYOD1 expression level was significantly higher in TW groups than that in TR groups(P<0.05). The expression level of FN1 decreased significantly with soybean oil supplemented in diet(P<0.05), whereas FGF2 and COL3A1 expression levels were affected by the interaction effects between dietary soybean oil supplementation and birth types(0.050.05). In conclusion, twin born lambs contain more collagen in muscle than triplet born lambs, and soybean oil supplementation show a trend to increase the collagen content in muscle. Moreover, dietary soybean oil supplementation and birth types are combined together to regulate the content of collagen metabolism enzymes and related genes expression level, and then modulate the collagen content in muscle.
This study aimed to investigate the effects of dietary soybean oil supplementation and different birth types on collagen characteristics and related genes expression in muscle of lamb. Sixty-four male Hu lambs with twin born(TW, 32 lambs) and triplet born(TR, 32 lambs) types weaned at 2 months old were selected and fed basal diet(CON) or diet with 2% soybean oil supplemented(SBO). Lambs were randomly divided into 4 treatments(TW-CON, TW-SBO, TR-CON, TR-SBO) according to 2×2 factorial experimental design, each treatment had 16 biological replication, and 1 lamb in each replication. Lambs were slaughtered after 90 days of feeding, and longissimus dorsi muscle samples were collected for detecting the content of collagen, enzymes activity and muscle development-related genes expression level. The results indicated that: 1) The collagen type Ⅰ content in TW groups was significantly higher than that in TR groups(P<0.05). Dietary soybean oil supplementation increased collagen content(0.05
引文
[1] NISHIMURA T.The role of intramuscular connective tissue in meat texture[J].Anim Sci J,2010,81(1):21-27.
[2] VAN DER REST M,GARRONE R.Collagen family of proteins[J].FASEB J,1991,5(13):2814-2823.
[3] PURSLOW P P.Intramuscular connective tissue and its role in meat quality[J].Meat Sci,2005,70(3):435-447.
[4] JENNISKENS Y M,KOEVOET W,DE BART A C W,et al.Biochemical and functional modulation of the cartilage collagen network by IGF1,TGFβ2 and FGF2[J].Osteoarthritis Cartilage,2006,14(11):1136-1146.
[5] LIGHT N,CHAMPION A E,VOYLE C,et al.The role of epimysial,perimysial and endomysial collagen in determining texture in six bovine muscles[J].Meat Sci,1985,13(3):137-149.
[6] VISSE R,NAGASE H.Matrix metalloproteinases and tissue inhibitors of metalloproteinases:structure,function,and biochemistry[J].Circ Res,2003,92(8):827-839.
[7] HUANG Y,ZHAOJ X,YAN X,et al.Maternal obesity enhances collagen accumulation and cross-linking in skeletal muscle of ovine offspring[J].PLoS One,2012,7(2):e31691.
[8] THERKILDSEN M,RIIS B,KARLSSON A,et al.Compensatory growth response in pigs,muscle protein turn-over and meat texture:effects of restriction/realimentation period[J].Anim Sci,2002,75(3):367-377.
[9] COHEN-ZINDER M,ORLOV A,TROFIMYUK O,et al.Dietary supplementation of Moringa oleifera silage increases meat tenderness of Assaf lambs[J].Small Rumin Res,2017,151:110-116.
[10] 赵俊星,刘向东,金亚倩,等.日粮中添加酿酒葡萄渣对绵羊肉品质及肌肉抗氧化性的影响[J].畜牧兽医学报,2017,48(8):1481-1490.ZHAO J X,LIU X D,JIN Y Q,et al.Effect of dietary wine grape pomace supplementation on the antioxidant ability and meat quality of lambs[J].Acta Veterinaria et Zootechnica Sinica,2017,48(8):1481-1490.(in Chinese)
[11] BEKHIT A E D A,CARNE A,HA M,et al.Physical interventions to manipulate texture and tenderness of fresh meat:a review[J].Int J Food Prop,2014,17(2):433-453.
[12] CHRIKI S,RENAND G,PICARD B,et al.Meta-analysis of the relationships between beef tenderness and muscle characteristics[J].Livest Sci,2013,155(2-3):424-434.
[13] PURSLOW P P.Contribution of collagen and connective tissue to cooked meat toughness;Some paradigms reviewed[J].Meat Sci,2018,144:127-134.
[14] BONNY S P F,GARDNER G E,PETHICK D W,et al.Biochemical measurements of beef are a good predictor of untrained consumer sensory scores across muscles[J].Animal,2015,9(1):179-190.
[15] MEZGEBO G B,MONAHAN F J,MCGEE M,et al.Biochemical and organoleptic characteristics of muscle from early and late maturing bulls in different production systems[J].Animal,2017,11(9):1636-1644.
[16] NISHIMURA T,HATTORI A,TAKAHASHI K.Structural changes in intramuscular connective tissue during the fattening of Japanese Black cattle:effect of marbling on beef tenderization[J].J Anim Sci,1999,77(1):93-104.
[17] CHO S H,KANG G,SEONG P,et al.Meat quality traits as a function of cow maturity[J].Anim Sci J,2017,88(5):781-789.
[18] MOREL P C H,MORRIS S T,KENYON P R.Effect of birth weight on survival in triplet-born lambs[J].Aust J Exp Agric,2008,48(7):984-987.
[19] 殷光田,王荣祥,陈书启,等.出生类型对湖羊羔羊体重变化的影响[J].安徽科技学院学报,2008,22(6):13-16.YIN G T,WANG R X,CHEN S Q,et al.Effect of birth type on body weight developments of lambs in Hu sheep[J].Journal of Anhui Science and Technology University,2008,22(6):13-16.(in Chinese)
[20] WELGUS H G,JEFFREY J J,EISEN A Z.The collagen substrate specificity of human skin fibroblast collagenase[J].J Biol Chem,1981,256(18):9511-9515.
[21] MATHEW S J,HANSEN J M,MERRELL A J,et al.Connective tissue fibroblasts and Tcf4 regulate myogenesis[J].Development,2011,138(2):371-384.
[22] NAGASE H,VISSE R,MURPHY G.Structure and function of matrix metalloproteinases and TIMPs[J].Cardiovasc Res,2006,69(3):562-573.
[23] BAILEY A J,SIMS T J.Chemistry of the collagen cross-links.Nature of the cross-links in the polymorphic forms of dermal collagen during development[J].Biochem J,1976,153(2):211-215.
[24] ARCHILE-CONTRERAS A C,MANDELL I B,PURSLOW P P.Disparity of dietary effects on collagen characteristics and toughness between two beef muscles[J].Meat Sci,2010,86(2):491-497.
[25] BONNET M,CASSAR-MALEK I,CHILLIARD Y,et al.Ontogenesis of muscle and adipose tissues and their interactions in ruminants and other species[J].Animal,2010,4(7):1093-1109.
[26] TAGA H,BONNET M,PICARD B,et al.Adipocyte metabolism and cellularity are related to differences in adipose tissue maturity between holstein and charolais or blond d'aquitaine fetuses[J].J Anim Sci,2011,89(3):711-721.
[27] ROTH J F,SHIKAMA N,HENZEN C,et al.Differential role of P300 and CBP acetyltransferase during myogenesis:P300 acts upstream of MyoD and Myf5[J].EMBO J,2003,22(19):5186-5196.
[28] TONG J F,YAN X,ZHU M J,et al.Maternal obesity downregulates myogenesis and β-catenin signaling in fetal skeletal muscle[J].Am J Physiol Endocrinol Metab,2009,296(4):E917-E924.
[29] YAN X,ZHU M J,XU W,et al.Up-regulation of Toll-like receptor 4/nuclear factor-κB signaling is associated with enhanced adipogenesis and insulin resistance in fetal skeletal muscle of obese sheep at late gestation[J].Endocrinology,2010,151(1):380-387.
[30] CHEN Y W,NAGARAJU K,BAKAY M,et al.Early onset of inflammation and later involvement of TGFβ in Duchenne muscular dystrophy[J].Neurology,2005,65(6):826-834.
[31] SALVADORI C,PETERS I R,DAY M J,et al.Muscle regeneration,inflammation,and connective tissue expansion in canine inflammatory myopathy[J].Muscle Nerve,2005,31(2):192-198.
[32] KENNEDY L,XU S W,CARTER D E,et al.Fibroblast adhesion results in the induction of a matrix remodeling gene expression program[J].Matrix Biol,2008,27(4):274-281.
[33] DUARTE M S,PAULINO P V R,DAS A K,et al.Enhancement of adipogenesis and fibrogenesis in skeletal muscle of Wagyu compared with Angus cattle[J].J Anim Sci,2013,91(6):2938-2946.
[34] CHAUVIGNé F,GABILLARD J C,WEIL C,et al.Effect of refeeding on IGFI,IGFII,IGF receptors,FGF2,FGF6,and myostatin mRNA expression in rainbow trout myotomal muscle[J].Gen Comp Endocrinol,2003,132(2):209-215.
[2] VAN DER REST M,GARRONE R.Collagen family of proteins[J].FASEB J,1991,5(13):2814-2823.
[3] PURSLOW P P.Intramuscular connective tissue and its role in meat quality[J].Meat Sci,2005,70(3):435-447.
[4] JENNISKENS Y M,KOEVOET W,DE BART A C W,et al.Biochemical and functional modulation of the cartilage collagen network by IGF1,TGFβ2 and FGF2[J].Osteoarthritis Cartilage,2006,14(11):1136-1146.
[5] LIGHT N,CHAMPION A E,VOYLE C,et al.The role of epimysial,perimysial and endomysial collagen in determining texture in six bovine muscles[J].Meat Sci,1985,13(3):137-149.
[6] VISSE R,NAGASE H.Matrix metalloproteinases and tissue inhibitors of metalloproteinases:structure,function,and biochemistry[J].Circ Res,2003,92(8):827-839.
[7] HUANG Y,ZHAOJ X,YAN X,et al.Maternal obesity enhances collagen accumulation and cross-linking in skeletal muscle of ovine offspring[J].PLoS One,2012,7(2):e31691.
[8] THERKILDSEN M,RIIS B,KARLSSON A,et al.Compensatory growth response in pigs,muscle protein turn-over and meat texture:effects of restriction/realimentation period[J].Anim Sci,2002,75(3):367-377.
[9] COHEN-ZINDER M,ORLOV A,TROFIMYUK O,et al.Dietary supplementation of Moringa oleifera silage increases meat tenderness of Assaf lambs[J].Small Rumin Res,2017,151:110-116.
[10] 赵俊星,刘向东,金亚倩,等.日粮中添加酿酒葡萄渣对绵羊肉品质及肌肉抗氧化性的影响[J].畜牧兽医学报,2017,48(8):1481-1490.ZHAO J X,LIU X D,JIN Y Q,et al.Effect of dietary wine grape pomace supplementation on the antioxidant ability and meat quality of lambs[J].Acta Veterinaria et Zootechnica Sinica,2017,48(8):1481-1490.(in Chinese)
[11] BEKHIT A E D A,CARNE A,HA M,et al.Physical interventions to manipulate texture and tenderness of fresh meat:a review[J].Int J Food Prop,2014,17(2):433-453.
[12] CHRIKI S,RENAND G,PICARD B,et al.Meta-analysis of the relationships between beef tenderness and muscle characteristics[J].Livest Sci,2013,155(2-3):424-434.
[13] PURSLOW P P.Contribution of collagen and connective tissue to cooked meat toughness;Some paradigms reviewed[J].Meat Sci,2018,144:127-134.
[14] BONNY S P F,GARDNER G E,PETHICK D W,et al.Biochemical measurements of beef are a good predictor of untrained consumer sensory scores across muscles[J].Animal,2015,9(1):179-190.
[15] MEZGEBO G B,MONAHAN F J,MCGEE M,et al.Biochemical and organoleptic characteristics of muscle from early and late maturing bulls in different production systems[J].Animal,2017,11(9):1636-1644.
[16] NISHIMURA T,HATTORI A,TAKAHASHI K.Structural changes in intramuscular connective tissue during the fattening of Japanese Black cattle:effect of marbling on beef tenderization[J].J Anim Sci,1999,77(1):93-104.
[17] CHO S H,KANG G,SEONG P,et al.Meat quality traits as a function of cow maturity[J].Anim Sci J,2017,88(5):781-789.
[18] MOREL P C H,MORRIS S T,KENYON P R.Effect of birth weight on survival in triplet-born lambs[J].Aust J Exp Agric,2008,48(7):984-987.
[19] 殷光田,王荣祥,陈书启,等.出生类型对湖羊羔羊体重变化的影响[J].安徽科技学院学报,2008,22(6):13-16.YIN G T,WANG R X,CHEN S Q,et al.Effect of birth type on body weight developments of lambs in Hu sheep[J].Journal of Anhui Science and Technology University,2008,22(6):13-16.(in Chinese)
[20] WELGUS H G,JEFFREY J J,EISEN A Z.The collagen substrate specificity of human skin fibroblast collagenase[J].J Biol Chem,1981,256(18):9511-9515.
[21] MATHEW S J,HANSEN J M,MERRELL A J,et al.Connective tissue fibroblasts and Tcf4 regulate myogenesis[J].Development,2011,138(2):371-384.
[22] NAGASE H,VISSE R,MURPHY G.Structure and function of matrix metalloproteinases and TIMPs[J].Cardiovasc Res,2006,69(3):562-573.
[23] BAILEY A J,SIMS T J.Chemistry of the collagen cross-links.Nature of the cross-links in the polymorphic forms of dermal collagen during development[J].Biochem J,1976,153(2):211-215.
[24] ARCHILE-CONTRERAS A C,MANDELL I B,PURSLOW P P.Disparity of dietary effects on collagen characteristics and toughness between two beef muscles[J].Meat Sci,2010,86(2):491-497.
[25] BONNET M,CASSAR-MALEK I,CHILLIARD Y,et al.Ontogenesis of muscle and adipose tissues and their interactions in ruminants and other species[J].Animal,2010,4(7):1093-1109.
[26] TAGA H,BONNET M,PICARD B,et al.Adipocyte metabolism and cellularity are related to differences in adipose tissue maturity between holstein and charolais or blond d'aquitaine fetuses[J].J Anim Sci,2011,89(3):711-721.
[27] ROTH J F,SHIKAMA N,HENZEN C,et al.Differential role of P300 and CBP acetyltransferase during myogenesis:P300 acts upstream of MyoD and Myf5[J].EMBO J,2003,22(19):5186-5196.
[28] TONG J F,YAN X,ZHU M J,et al.Maternal obesity downregulates myogenesis and β-catenin signaling in fetal skeletal muscle[J].Am J Physiol Endocrinol Metab,2009,296(4):E917-E924.
[29] YAN X,ZHU M J,XU W,et al.Up-regulation of Toll-like receptor 4/nuclear factor-κB signaling is associated with enhanced adipogenesis and insulin resistance in fetal skeletal muscle of obese sheep at late gestation[J].Endocrinology,2010,151(1):380-387.
[30] CHEN Y W,NAGARAJU K,BAKAY M,et al.Early onset of inflammation and later involvement of TGFβ in Duchenne muscular dystrophy[J].Neurology,2005,65(6):826-834.
[31] SALVADORI C,PETERS I R,DAY M J,et al.Muscle regeneration,inflammation,and connective tissue expansion in canine inflammatory myopathy[J].Muscle Nerve,2005,31(2):192-198.
[32] KENNEDY L,XU S W,CARTER D E,et al.Fibroblast adhesion results in the induction of a matrix remodeling gene expression program[J].Matrix Biol,2008,27(4):274-281.
[33] DUARTE M S,PAULINO P V R,DAS A K,et al.Enhancement of adipogenesis and fibrogenesis in skeletal muscle of Wagyu compared with Angus cattle[J].J Anim Sci,2013,91(6):2938-2946.
[34] CHAUVIGNé F,GABILLARD J C,WEIL C,et al.Effect of refeeding on IGFI,IGFII,IGF receptors,FGF2,FGF6,and myostatin mRNA expression in rainbow trout myotomal muscle[J].Gen Comp Endocrinol,2003,132(2):209-215.