Vimentin在子宫内膜腺癌组织中的表达及其临床意义
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摘要
目的探讨Vimentin在人子宫内膜腺癌中的表达及其临床意义。方法采用免疫组化方法及Western-blot方法研究子宫内膜腺癌组织及正常子宫内膜组织中Vimentin的表达。结果 Vimentin蛋白表达水平在子宫内膜癌组织中显著高于正常子宫内膜组织(P<0.05)。Vimentin在子宫内膜癌组织的表达与年龄,FIGO分期,癌症分化程度,病灶大小以及肌层浸润深度密切相关(P<0.05),并与雌激素受体(Estrogen Receptor,ER),孕激素受体(Progesterone Receptor,PR)呈负相关。结论 Vimentin在子宫内膜癌中过表达,提示其可能参与子宫内膜癌的发病机制。
Objective To investigate the expression of Vimentin protein in human endometrial carcinoma and its relationship with clinicopathological parameters. Methods Vimentin protein expression levels in endometrial carcinoma and normal endometrial tissues were detected and analyzed by immuohistochemstry and Western-blot. Results Vimentin protein expression levels were significantly higher in endometrial carcinoma tissues than in normal endometrial tissues(P<0.05). Vimentin protein expression was significantly correlated with age, FIGO stage, differentiation, tumor size and infiltration depth of the endometrial carcinoma, with significantly negative correlation between Vimentin and ER, PR. Conclusion The overexpression of Vimentin might be involved in the pathogenesis of endometrial carcinoma.
Objective To investigate the expression of Vimentin protein in human endometrial carcinoma and its relationship with clinicopathological parameters. Methods Vimentin protein expression levels in endometrial carcinoma and normal endometrial tissues were detected and analyzed by immuohistochemstry and Western-blot. Results Vimentin protein expression levels were significantly higher in endometrial carcinoma tissues than in normal endometrial tissues(P<0.05). Vimentin protein expression was significantly correlated with age, FIGO stage, differentiation, tumor size and infiltration depth of the endometrial carcinoma, with significantly negative correlation between Vimentin and ER, PR. Conclusion The overexpression of Vimentin might be involved in the pathogenesis of endometrial carcinoma.
引文
[1]王丽琴.子宫内膜癌临床诊治的研究进展[J].中国肿瘤临床与康复,2012,19(5):472-474.
[2]潘燕,韩婧,张晔,等.Vimentin在肿瘤转移中的作用及药物研究进展[J].生理科学进展,2010,41(6):413-416.
[3]池丽敏,肖鹏,陈定科,等.Vimentin蛋白在子宫内膜样腺癌组织中的表达及其临床意义[J].现代医学,2015,43(10):1216-1218.
[4]Fuyuhiro Y,Yashiro M,Noda S,et al.Clinical significance of vimentin-positive gastric cancer cells[J].Anticancer Res,2010,30(12):5239-5243.
[5]Zhao Y,Yan Q,Long X,et al.Vimentin affects the mobility and invasiveness of prostate cancer cells[J].Cell Biochem Funct,2008,26(5):571-577.
[6]Yanaranop M,Ayuwat S,Nakrangsee S.Differential diagnosis between primary endocervical and endometrial adenocarcinoma using immunohistochemical staining of estrogen receptor,Vimentin,carcinoembryonic antigen and p16[J].J Med Assoc Thai,2016,99(Suppl 2):S106-115.
[7]Desouki MM,Kallas SJ,Khabele D,et al.Differential vimentin expression in ovarian and uterine corpus endometrioid adenocarcinomas:diagnostic utility in distinguishing double primaries from metastatic tumors[J].Int J Gynecol Pathol,2014,33(3):274-281.
[8]耿文文,张斌,李丹华,等.成纤维细胞生长因子-2逆转上皮间质转化机制[J].中华实验外科杂志,2013,30:99-101.
[9]Chernyatina AA1,Nicolet S,Aebi U,et al.Atomic structure of the vimentin centralα-helical domain and its implications for intermediate filament assembly[J].Proc Natl Acad Sci USA,2012,109:13620-13625.
[10]Senol S,Sayar I,Ceyran AB,et al.Stromal clues in endometrial carcinoma:loss of expression ofβ-Catenin,epithelialmesenchymal transition regulators,and estrogen-progesterone receptor[J].Int J Gynecol Pathol,2016,35(3):238-248.
[11]Chen X,Liu X,Lang H,et al.S100 calcium-binding protein A6promotes epithelial-mesenchymal transition throughβ-catenin in pancreatic cancer cell line[J].PLo S One,2015,10(3):e0121319.
[2]潘燕,韩婧,张晔,等.Vimentin在肿瘤转移中的作用及药物研究进展[J].生理科学进展,2010,41(6):413-416.
[3]池丽敏,肖鹏,陈定科,等.Vimentin蛋白在子宫内膜样腺癌组织中的表达及其临床意义[J].现代医学,2015,43(10):1216-1218.
[4]Fuyuhiro Y,Yashiro M,Noda S,et al.Clinical significance of vimentin-positive gastric cancer cells[J].Anticancer Res,2010,30(12):5239-5243.
[5]Zhao Y,Yan Q,Long X,et al.Vimentin affects the mobility and invasiveness of prostate cancer cells[J].Cell Biochem Funct,2008,26(5):571-577.
[6]Yanaranop M,Ayuwat S,Nakrangsee S.Differential diagnosis between primary endocervical and endometrial adenocarcinoma using immunohistochemical staining of estrogen receptor,Vimentin,carcinoembryonic antigen and p16[J].J Med Assoc Thai,2016,99(Suppl 2):S106-115.
[7]Desouki MM,Kallas SJ,Khabele D,et al.Differential vimentin expression in ovarian and uterine corpus endometrioid adenocarcinomas:diagnostic utility in distinguishing double primaries from metastatic tumors[J].Int J Gynecol Pathol,2014,33(3):274-281.
[8]耿文文,张斌,李丹华,等.成纤维细胞生长因子-2逆转上皮间质转化机制[J].中华实验外科杂志,2013,30:99-101.
[9]Chernyatina AA1,Nicolet S,Aebi U,et al.Atomic structure of the vimentin centralα-helical domain and its implications for intermediate filament assembly[J].Proc Natl Acad Sci USA,2012,109:13620-13625.
[10]Senol S,Sayar I,Ceyran AB,et al.Stromal clues in endometrial carcinoma:loss of expression ofβ-Catenin,epithelialmesenchymal transition regulators,and estrogen-progesterone receptor[J].Int J Gynecol Pathol,2016,35(3):238-248.
[11]Chen X,Liu X,Lang H,et al.S100 calcium-binding protein A6promotes epithelial-mesenchymal transition throughβ-catenin in pancreatic cancer cell line[J].PLo S One,2015,10(3):e0121319.