摘要
为弄清辽宁某蛋鸡场鸡只产蛋量突然下降,死亡率上升及部分剖检鸡只肝脏肿大出血的原因,对采集的发病鸡肝脏组织进行病理学观察,同时对肠道内容物进行禽戊型肝炎病毒(Hepatitis E virus,HEV)核酸片段的RT-PCR检测。肝脏的组织病理学观察发现,肝细胞坏死和汇管区周围淋巴细胞浸润;RT-PCR从肠道内容物中成功扩增到禽HEV ORF2基因的部分片段;基因序列同源性分析结果表明,其与国内禽HEV参考株的序列同源性为97.5%~99.6%,而与其他国家的HEV序列同源性为77.9%~97.6%;进化树分析表明,该序列(命名为China 15-LN)与国内其他地区及欧洲的部分分离株在同一进化分支上,同属禽HEV基因3型。
引文
[1]Meng X J.Hepatitis E virus(hepevirus)[M].Encyclopedia of Virology:5 vols 3 rd ed Elsevier Oxford,2008:377-383.
[2]Payne C J,Ellis T M,Wilcox G E,et al.Sequence data suggests big liver and spleen disease virus(BLSV)is genetically related to hepatitis E virus[J].Veterinary Microbiology,1999,68(1-2):119-125.
[3]Haqshenas G,Shivaprasad H L,Meng X J,et al.Genetic identification and characterization of a novel virus relate d to human hepatitis E virus from chickens with hepatitis-splenomegaly syndrome in the United States[J].Journal of General Virology,2001,82(Pt 10):2449-2462.
[4]Huang F F,Haqshenas G,Meng X J,et al.Heterogeneity and seroprevalence of a newly identified avian hepatitis E virus from chickens in the United States[J].Journal of Clinical Microbiology,2002,40(11):4197-4202.
[5]Agunos A C,Yoo D,Hunter D B,et al.Avian hepatitis E virus in an outbreak of hepatitis-splenomegaly syndrome and fatty liver haemorrhage syndrome in two flaxseed-fed layer flocks in Ontario[J].Avian Pathology,2006,35(5):404-412.
[6]Morrow C J,Samu G,Hess M,et al.Avian hepatitis E virus infection and possible associated clinical disease in broiler breeder flocks in Hungary[J].Avian Pathology,2008,37(5):527-535.
[7]Zhao Q,Zhou E M,Sun Y N,et al.Analysis of avian hepatitis Evirus from chickens,China[J].Emerging Infectious Diseases,2010,16(9):1469-1472.
[8]Sprygin A V,Nikonova Z B,Zinyakov N G.Avian hepatitis E virus identified in Russian chicken flocks exhibits high genetic divergence based on the ORF2 capsid gene[J].Avian Pathology,2012,41(5):459-463.
[9]Ingrid W.-Y.Hsu and Hsiang-Jung Tsai.Avian Hepatitis E Virus in Chickens,Taiwan[J].Emerging Infectious Diseases,2013,20(1):149-151.
[10]Sun Z F,Larsen C T,Dunlop A,et al.Genetic identification of avian hepatitis E virus(avian HEV)from healthy chicken flocks and characterization of the capsid gene of 14 avian HEV isolates from chickens with hepatitis-splenomegaly syndrome in different geographical regions of the United States[J].J Gen Virol,2004,85(Pt 3):693-700.
[11]Billam P,Huang F F,Sun Z F,et al.Systematic pathogenesis and replication of avian hepatitis E virus specific-pathogen free adult chickens[J].J Virol,2005,79(6):3429-3437.
[12]王林,刘青,徐希水.鸡三种肿瘤性疾病的鉴别诊断及预防措施[J].家禽科学,2008(2):30-31.