GHR基因9-bp InDel与陕北白绒山羊体重和生长性状的关联研究
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摘要
生长激素受体(growth hormone receptor,GHR)是生长激素(GH)的特定跨膜受体,对动物正常的生长发育具有重要作用。该试验随机选择532只陕北白绒山羊,通过降落PCR (Touch down PCR)方法鉴定陕北白绒山羊GHR基因第一内含子存在的9bp插入缺失(InDel)情况并分析其与体重、生长性状的相关性。研究发现,陕北白绒山羊GHR基因共出现II、ID、DD 3种基因型;相关分析结果表明,在体重、髋宽、体高、荐高和胸深指标上,基因型ID或DD个体相比较II型个体更具显著性优势(P<0.05),说明该位点等位基因D更有利于山羊的生长发育。研究表明GHR基因可作为陕北白绒山羊体重和生长性状的候选基因,为陕北白绒山羊产业快速高效发展提供科学理论依据。
Growth hormone receptor(GHR)is a specific transmembrane receptor of growth hormone(GH),which plays an important role in normal growth and development of animals.This study identified the insertion/deletion(InDel)site of GHRgene of Shanbei White Cashmere goats by Touch down PCR in532 individuals randomly,and to evaluate the correlation of this InDel site with body weight and growth traits.Results show there are three kinds of genotypes(II,ID,DD).Correlation analysis results show that in terms of body weight index,hip width index,body height and recommendation height,chest depth traits,ID or DD individuals are significantly better than II individuals(P<0.05).This indicates that the allele D is more favorable for the growth and development of goats.The study indicates that GHR gene can be used as a candidate gene for body weight and growth characteristics of Shanbei White Cashmere goats,and it provides ascientific theoretical basis for the rapid and efficient development of Shanbei white cashmere goat industry.
Growth hormone receptor(GHR)is a specific transmembrane receptor of growth hormone(GH),which plays an important role in normal growth and development of animals.This study identified the insertion/deletion(InDel)site of GHRgene of Shanbei White Cashmere goats by Touch down PCR in532 individuals randomly,and to evaluate the correlation of this InDel site with body weight and growth traits.Results show there are three kinds of genotypes(II,ID,DD).Correlation analysis results show that in terms of body weight index,hip width index,body height and recommendation height,chest depth traits,ID or DD individuals are significantly better than II individuals(P<0.05).This indicates that the allele D is more favorable for the growth and development of goats.The study indicates that GHR gene can be used as a candidate gene for body weight and growth characteristics of Shanbei White Cashmere goats,and it provides ascientific theoretical basis for the rapid and efficient development of Shanbei white cashmere goat industry.
引文
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[19]WANG K,YAN H,XU H,et al.A novel indel within goat casein alpha S1gene is significantly associated with litter size[J].Gene,2018,671(2018):161-169.
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[21]黄治国,谢庄.绵羊肌肉生长激素受体基因表达的发育性变化研究[J].安徽农业科学,2009,37(9):3 977-3 979.
[22]张磊,王燕燕,周占琴,等.卵巢摘除对山羊血清GH水平和组织中GHR基因表达的影响[J].中国农业科学,2014,47(1):199-208.
[23]张清峰,许尚忠.牛GHR10基因HindIII酶切多态性与体尺、体重指标的相关性分析[J].中国畜牧兽医,2009,26(8):64-67.
[24]DIAS C,GIORDANO M,FRECHETTE R,et al.Genetic variations at the human growth hormone receptor(GHR)gene locus are associated with idiopathic short stature.Journal of Cellular and Molecular Medicine,2017,21(11):2 985-2 999.
[25]金连丰,于广璞,孙霞,等.牛GHR基因与miR-139靶向关系的验证[J].畜牧兽医学报,2016,47(12):2 398-2 404.
[26]ZHU J,ZHU J,ZHU D,et al.MicroRNA‐363inhibits angiogenesis,proliferation,invasion,and migration of renal cell carcinoma via inactivation of the Janus tyrosine kinases 2-signal transducers and activators of transcription 3axis by suppressing growth hormone receptor gene[J].Journal of cellular physiology,2019,234(3):2 581-2 592.
[27]海汀,柴志欣,钟金城.麦洼牦牛GH、GHR、GHSR基因的SNPs检测及其与体尺性状的关联分析[J].畜牧兽医学报,2017,48(4):605-617.
[28]蒋钦杨,黄艳娜,韦英明,等.德保矮马生长激素受体基因RCR-SSCP分析[J].黑龙江畜牧兽医,2013(3):3-7.
[29]张玲,董飚,段修军,等.日粮营养水平对番鸭GH、GHR基因表达影响的研究[J].科学试验与研究,2018(2):46-53.
[30]武秀香,杨章平,毛永江,等.GHR基因遗传变异及其对中国西门塔尔牛胴体性状的效应分析[J].扬州大学学报,2015,26(4):31-34.
[2]乔业宏,纪敏.陕北白绒山羊防疫与保健技术[J].畜禽业,2018(7):18-18.
[3]韩聪,方乾,李浩,等.不同月龄阶段陕北白绒山羊生精细胞发育规律研究[J].家畜生态学报,2018,39(4):43-48.
[4]郭礼祥,王永军,黄艳平,等.控制光照对陕北白绒山羊体重和绒毛长度的影响研究[J].家畜生态学报,2012,33(5):20-22.
[5]YANG Q,ZHANG S,LIU L,et al.Application of mathematical expectation(ME)strategy for detecting low frequency mutations:An example for evaluating 14-bp insertion/deletion(indel)within the bovine PRNP gene[J].Prion,2016,10(5):409-419.
[6]马青,刘彩凤,施安,等.GH和GHR基因多态性与滩羊生长性状的关联分析[J].黑龙江畜牧兽医,2015(11):31-39.
[7]ARMSTRONG E,CIAPPESONI G,IRIARTE W,et al.Novel genetic polymorphisms associated with carcass traits in grazing Texel sheep[J].Meat Science,2018,145:202-208.
[8]冉雪琴,赵星艳,王嘉福,等.贵州矮马(Equus caballus)生长激素受体基因5个单核苷酸位点的多态性研究[J].畜牧兽医学报,2015,46(11):1 974-1 986.
[9]刘芳,郝荣超,王国华,等.GHR基因第10外显子与塞北兔生长发育性能相关性研究[J].基因组学与应用生物学,2015,34(9):1 865-1 869.
[10]杨慧,陈宝剑,兰干球,等.猪GHR基因两个SNPs位点多态性与生长性状的关联分析[J].南方农业学报,2014,45(11):2 052-2 057.
[11]YANG Q,YAN H,LI J,et al.A novel 14‐bp duplicated deletion within goat GHR gene is significantly associated with growth traits and litter size[J].Animal genetics,2017,48(4):499-500.
[12]常新侠,王树迎,于学浩,等.济宁青山羊生后乳腺GHR和IGF-IR的分布及其mRNA表达的发育性变化[J].中国兽医学报,2016,36(7):1 234-1 240.
[13]杨洁,赫佳,王丹碧,等.InDel标记的研究和应用[J].进展生物多样性,2016,24(2):237-243.
[14]CUI Y,YAN H,WANG K,et al.Insertion/deletion within the KDM6Agene is significantly associated with litter size in goat[J].Frontiers in genetics,2018,9:91.
[15]WANG X,YANG Q,WANG K,et al.A novel 12‐bp indel polymorphism within the GDF9gene is significantly associated with litter size and growth traits in goats[J].Animal genetics,2017,48(6):735-736.
[16]XU H,ZHANG S,ZHANG X,et al.Evaluation of novel SNPs and haplotypes within the ATBF1gene and their effects on economically important production traits in cattle[J].Archives Animal Breeding,2017,60(3):285-296.
[17]YANG Q,ZHANG S L,LI J,et al.Development of a touch-down multiplex PCR method for simultaneously rapidly detecting three novel insertion/deletions(indels)within one gene:an example for goat GHR gene[J].Animal biotechnology,2018:1-6.DOI:10.1080/10495398.2018.1517770.
[18]赵海谕.奶山羊PITX1、PITX2基因的遗传变异、mRNA表达及DNA甲基化修饰对泌乳性能的影响[D].杨凌:西北农林科技大学,2014.
[19]WANG K,YAN H,XU H,et al.A novel indel within goat casein alpha S1gene is significantly associated with litter size[J].Gene,2018,671(2018):161-169.
[20]WANG X,YANG Q,WANG K,et al.Two strongly linked single nucleotide polymorphisms(Q320P and V397I)in GDF9gene are associated with litter size in cashmere goats[J].Theriogenology,2019,125:115-121.
[21]黄治国,谢庄.绵羊肌肉生长激素受体基因表达的发育性变化研究[J].安徽农业科学,2009,37(9):3 977-3 979.
[22]张磊,王燕燕,周占琴,等.卵巢摘除对山羊血清GH水平和组织中GHR基因表达的影响[J].中国农业科学,2014,47(1):199-208.
[23]张清峰,许尚忠.牛GHR10基因HindIII酶切多态性与体尺、体重指标的相关性分析[J].中国畜牧兽医,2009,26(8):64-67.
[24]DIAS C,GIORDANO M,FRECHETTE R,et al.Genetic variations at the human growth hormone receptor(GHR)gene locus are associated with idiopathic short stature.Journal of Cellular and Molecular Medicine,2017,21(11):2 985-2 999.
[25]金连丰,于广璞,孙霞,等.牛GHR基因与miR-139靶向关系的验证[J].畜牧兽医学报,2016,47(12):2 398-2 404.
[26]ZHU J,ZHU J,ZHU D,et al.MicroRNA‐363inhibits angiogenesis,proliferation,invasion,and migration of renal cell carcinoma via inactivation of the Janus tyrosine kinases 2-signal transducers and activators of transcription 3axis by suppressing growth hormone receptor gene[J].Journal of cellular physiology,2019,234(3):2 581-2 592.
[27]海汀,柴志欣,钟金城.麦洼牦牛GH、GHR、GHSR基因的SNPs检测及其与体尺性状的关联分析[J].畜牧兽医学报,2017,48(4):605-617.
[28]蒋钦杨,黄艳娜,韦英明,等.德保矮马生长激素受体基因RCR-SSCP分析[J].黑龙江畜牧兽医,2013(3):3-7.
[29]张玲,董飚,段修军,等.日粮营养水平对番鸭GH、GHR基因表达影响的研究[J].科学试验与研究,2018(2):46-53.
[30]武秀香,杨章平,毛永江,等.GHR基因遗传变异及其对中国西门塔尔牛胴体性状的效应分析[J].扬州大学学报,2015,26(4):31-34.