基于代谢组学技术研究老年原发性高血压肾气亏虚证本质的可行性探讨
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摘要
代谢组学作为后基因时代的新兴"组学",是一项对生物系统内所有代谢产物进行定性、定量分析的系统性研究技术。它所研究的代谢物是机体受到刺激干扰后整体功能状态的"生化表型",因此代谢组学具备系统、动态、整体、分析的特点,与中医学整体观、系统观以及辨证论治思想相契合。老年高血压是原发性高血压的临床亚型之一,具有特征性的发病机制和证候特点,肾气亏虚是其病理机制中的重要环节,代谢组学技术为其证候本质的探索开拓了一条新思路。分析代谢组学技术与中医证候本质研究的契合点,就代谢组学技术研究老年原发性高血压肾气亏虚证本质的可行性进行探讨。
As an emerging "omics" in the post-genomic era,metabonomics is a systematic research technology for qualitative and quantitative analysis of all metabolites in a biological matrix.The metabolites studied by the organism are the "biochemical phenotype" of the whole functional state after the body is disturbed by stimulation,so metabonomics has the characteristics of system, dynamics, entirety and analysis,which accord with the holistic view,the system view and the treatment according to syndrome differentiation theory of traditional Chinese medicine. Elderly hypertension is one of the clinical subtypes of hypertension,which has characteristic pathogenesis and syndrome characteristics,the kidney qi deficiency is a significant part of its pathology mechanisms,and metabonomics technology has opened up a new way to explore the essence of this syndrome. In this paper,the conjunction between metabonomics and the essence of TCM syndromes were analyzed,the feasibility of the research on the essence of elderly hypertension with kidney qi deficiency syndrome based on metabonomics technology was also discussed.
As an emerging "omics" in the post-genomic era,metabonomics is a systematic research technology for qualitative and quantitative analysis of all metabolites in a biological matrix.The metabolites studied by the organism are the "biochemical phenotype" of the whole functional state after the body is disturbed by stimulation,so metabonomics has the characteristics of system, dynamics, entirety and analysis,which accord with the holistic view,the system view and the treatment according to syndrome differentiation theory of traditional Chinese medicine. Elderly hypertension is one of the clinical subtypes of hypertension,which has characteristic pathogenesis and syndrome characteristics,the kidney qi deficiency is a significant part of its pathology mechanisms,and metabonomics technology has opened up a new way to explore the essence of this syndrome. In this paper,the conjunction between metabonomics and the essence of TCM syndromes were analyzed,the feasibility of the research on the essence of elderly hypertension with kidney qi deficiency syndrome based on metabonomics technology was also discussed.
引文
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[13]阮小芬,唐晓婷.老年高血压病中医证候与相关危险因素及心脑血管事件相关性研究[J].中华中医药杂志,2016,31(3):1025-1028.
[14]王阶,熊兴江,刘巍.补肾法治疗高血压病[J].中国中药杂志,2013,38(9):1277-1279.
[15]董巧稚,逯金金,李岩,等.补肾降压方对肾气亏虚型高血压病的疗效观察[J].中西医结合心脑血管病杂志,2017,15(9):1034-1036.
[16]刘华.补肾益气活血汤对高血压病相关指标的影响[J].浙江中西医结合杂志,2003,13(1):7-9.
[2]SUN J,SCHNACKENBERG L K,BEGER R D.Studies of acetaminophen and metabolites in urine and their correlations with toxicity using metabolomics[J].Drub Metub Lett,2009,3(7):130-136.
[3]YANG Y,LI C,NIE X,et al.Metabonomic studies of human hepatocellular carcinoma using high-resolution magicangle spinning1H NMR spectroscopy in conjunction with multivariate data analysis[J].Journal of Proteome Research,2007,6(7):2605-2614.
[4]ZHANG A H,SUN H,YAN G L,et al.Metabolomics study of type 2 diabetes using ultra-performance LC-ESl/quadrupole-TOF high-definition MS coupled with pattern recognition methods[J].Journal of Proteome Research,2014,70(1):117-128.
[5]WANG X J,SUN H,ZHANG A H,et al.Potential role of metabolomics apporoaches in the area of traditional Chinese medicine:as pillars of the bridge between Chinese and Western medicine[J].Journal of Pharmaceutical and Biomedical Analysis,2011,55(5):859-868.
[6]蒋海强,马斌,聂磊,等.高血压病肝阳上亢证患者尿液样品的核磁共振谱代谢组学研究[J].浙江中医药大学学报,2011,35(5):662-669.
[7]蒋海强,聂磊,李运伦.气质联用技术监测高血压肝阳上亢患者与健康志愿者血清脂肪酸成分的差异[J].辽宁中医杂志,2011,38(6):1052-1054.
[8]程鹏,陈泽奇,王东生,等.冠心病痰浊证与气虚证的代谢组学研究[J].中国中西医结合杂志,2015,35(2):193-197.
[9]朱明丹,杜武勋,姜民,等.心肌梗死恢复期气虚血瘀证的代谢组学研究[J].时珍国医国药,2016,27(6):1527-1530.
[10]董飞侠,黄迪,何立群,等.Ⅲ期慢性肾病肾阳虚证患者尿液代谢组学特征的研究[J].中华中医药杂志,2008,23(12):1109-1113.
[11]郭伟星.补益肾气法治疗老年人高血压的研究[J].山东中医学院学报,1994,18(5):304-310.
[12]胡敬宝,王洪燕,黄秀玲.补肾活血治疗老年人高血压病的临床研究[J].吉林中医药,2001,21(2):8-9.
[13]阮小芬,唐晓婷.老年高血压病中医证候与相关危险因素及心脑血管事件相关性研究[J].中华中医药杂志,2016,31(3):1025-1028.
[14]王阶,熊兴江,刘巍.补肾法治疗高血压病[J].中国中药杂志,2013,38(9):1277-1279.
[15]董巧稚,逯金金,李岩,等.补肾降压方对肾气亏虚型高血压病的疗效观察[J].中西医结合心脑血管病杂志,2017,15(9):1034-1036.
[16]刘华.补肾益气活血汤对高血压病相关指标的影响[J].浙江中西医结合杂志,2003,13(1):7-9.