显齿蛇葡萄中黄酮组成对相关生物活性的影响
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摘要
研究显齿蛇葡萄中不同黄酮组成对α-葡萄糖苷酶的抑制活性及DPPH·清除能力。15份不同显齿蛇葡萄叶,甲醇超声辅助法制备供试品;α-葡萄糖苷酶抑制和DPPH·清除模型评价相关生物活性;拟复方验证原样品的相应生物活性。结果表明:对α-葡萄糖苷酶抑制活性最好的植株为ZF3号, IC_(50)为0.007 mg/mL;α-葡萄糖苷酶IC_(50)与二氢杨梅素、杨梅素含量呈极显著正相关(p<0.01),相关系数分别为0.724, 0.870;与杨梅苷含量呈显著负相关(p<0.05),相关系数为0.604。拟复方样品的活性与之对应原植物样品相当,但略低于原样品。显齿蛇葡萄叶中二氢杨梅素、杨梅素和杨梅苷对其α-葡萄糖苷酶的抑制活性有显著影响,而对DPPH·清除能力影响不显著;二氢杨梅素含量低的显齿蛇葡萄叶适宜开发降糖产品。
Inhibition of flavonoids composition on α-glucosidase activity and DPPH· clearance in Ampelopsis grossedebtata grape. 15 kinds of grape leaves of Ampelopsis grossedebtata with different contents of dihydromyricetin, myricetin and myricitrin, prepared by ultrasonic assisted extraction of methanol; Evaluation of related biological activities by α-glucosidase inhibition model and DPPH· clearance model; Verification of the bioactivity of the original sample by compound method(according to the ratio of the three flavonoids in the sample). The results showed the α-glucosidase inhibitory activity and DPPH· scavenging ability of different flavonoids from Ampelopsis grossedebtata leaf extract. The contents of dihydromyricetin and myricetin were significantly positively correlated with their α-glucosidase IC_(50)(p<0.01), and the correlation coefficients were 0.724 and 0.870, respectively. The content of myricitrin was negatively correlated with α-glucosidase IC_(50)(p<0.05), and the correlation coefficient was 0.604. However, the correlation between the three flavonoids and DPPH· scavenging ability was weak and the effect was small. The activity of the compound sample was similar to that of the original plant sample, but slightly lower than the original sample. Dihydromyricetin, myricetin and myricitrin in Ampelopsis grossedebtata leaves had significant effects on the α-glucosidase inhibitory activity, but had no significant effect on DPPH· scavenging ability. The Ampelopsis grossedebtata leaves with low dihydromyricetin content were suitable for development of hypoglycemic products.
Inhibition of flavonoids composition on α-glucosidase activity and DPPH· clearance in Ampelopsis grossedebtata grape. 15 kinds of grape leaves of Ampelopsis grossedebtata with different contents of dihydromyricetin, myricetin and myricitrin, prepared by ultrasonic assisted extraction of methanol; Evaluation of related biological activities by α-glucosidase inhibition model and DPPH· clearance model; Verification of the bioactivity of the original sample by compound method(according to the ratio of the three flavonoids in the sample). The results showed the α-glucosidase inhibitory activity and DPPH· scavenging ability of different flavonoids from Ampelopsis grossedebtata leaf extract. The contents of dihydromyricetin and myricetin were significantly positively correlated with their α-glucosidase IC_(50)(p<0.01), and the correlation coefficients were 0.724 and 0.870, respectively. The content of myricitrin was negatively correlated with α-glucosidase IC_(50)(p<0.05), and the correlation coefficient was 0.604. However, the correlation between the three flavonoids and DPPH· scavenging ability was weak and the effect was small. The activity of the compound sample was similar to that of the original plant sample, but slightly lower than the original sample. Dihydromyricetin, myricetin and myricitrin in Ampelopsis grossedebtata leaves had significant effects on the α-glucosidase inhibitory activity, but had no significant effect on DPPH· scavenging ability. The Ampelopsis grossedebtata leaves with low dihydromyricetin content were suitable for development of hypoglycemic products.
引文
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[16]冯淳,张妮,周大颖,等. HPLC测定显齿蛇葡萄叶中4种黄酮类化合物的含量[J/OL].食品工业科技:1-8[2018-08-06]. http://kns.cnki.net/kcms/detail/11.1759.TS.20180723.0944.006.html.
[2]周才碧,张敏星,穆瑞禄,等.藤茶有效成分及功效的研究进展[J].福建茶业, 2014(3):2-4.
[3]付明,黎晓英,王登宇,等.显齿蛇葡萄叶中黄酮类化合物的研究[J].中国药学杂志, 2015(7).
[4]王家胜,何磊磊,张妮,等.不同产地显齿蛇葡萄中二氢杨梅素测定[J].中成药, 2014, 36(1):146-149.
[5]熊皓平,何国庆,杨伟丽.显齿蛇葡萄生化成分分析[J].中国食品学报, 2004(3):68.
[6]冯涵,李娜,王华林,等.藤茶中黄酮类成分的功效研究进展[J].公共卫生与预防医学, 2018, 29(1):82-86.
[7]张斌.显齿蛇葡萄提取物的体内和体外降血糖效果[J].中国老年学志, 2017, 37(2):321-323.
[8]漆姣媚,蒋燕群,张杰,等.显齿蛇葡萄总黄酮降血糖作用研究[J].中国药学志, 2017, 52(19):1685-1690.
[9]王家胜.显齿蛇葡萄中黄酮化合物代谢及相关性研究[D].贵阳:贵州师范大学, 2015.
[10]汲军.显齿蛇葡萄叶提取黄酮化合物工艺研究[N].中国食品报, 2016-11-14(6).
[11] CHEN J, WU Y C, ZOU J W, et al.α-Glucosidase inhibition and antihyperglycemic activity of flavonodis from Ampelopsisgrossedentata and the flavonoid derivatives[J].Bioorganic&Medicinal Chemistry, 2016, 24:1488-1494.
[12]赖小燕,姜泽东,倪辉,等.茶花粉黄酮对α-葡萄糖苷酶抑制作用的研究[J].食品工业科技, 2016, 37(5):353-357.
[13]王斯慧,黄琬凌,陈庆松,等.芦丁、槲皮素对α-葡萄糖苷酶活性抑制研究[J].中国酿造, 2012, 31(1):133-135.
[14] ALESSANDRA C P, DANIEL G, NEIVA D R. Extraction of anthocyanins and polyphenols from black rice(Oryza sativaL)by modeling and assessing their reversibility and stability[J].Food Chemistry, 2016, 191:12-20.
[15]孔琪,玉秋萍,王家胜,等.不同二氢杨梅素含量的显齿蛇葡萄提取物的抗菌活性与清除DPPH自由基能力研究[J].食品工业科技, 2015, 36(5):87-90.
[16]冯淳,张妮,周大颖,等. HPLC测定显齿蛇葡萄叶中4种黄酮类化合物的含量[J/OL].食品工业科技:1-8[2018-08-06]. http://kns.cnki.net/kcms/detail/11.1759.TS.20180723.0944.006.html.