真/细菌对疏水性有机物的吸附及其表面特性
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摘要
比较了干燥后真菌Ophiostoma stenoceras LLC和细菌Pseudomonas veronii ZW菌体细胞的比表面积及其对不同疏水性有机化合物吸附性能,并利用BET、红外光谱(FTIR)和X射线电子能谱(XPS)对细胞表面进行了分析.结果表明,真菌LLC和细菌ZW菌体细胞的比表面积分别为15.8m~2/g,11.57m~2/g,真菌菌体细胞表面介孔较多,可以更有效地吸附有机化合物.在相同的生长阶段,真菌LLC的细胞表面疏水性(cellsurface hydrophobic,CSH)始终要大于细菌ZW;采用α-蒎烯作为唯一碳源培养时,处于对数生长期的真菌和细菌的CSH均有不同提升.干燥后真菌LLC菌体对各疏水性有机化合物吸附能力为乙酸乙酯>α-蒎烯>正己烷,即疏水性相对较低的化合物更容易被吸附.通过XPS和FTIR表征发现菌体LLC吸附有机化合物后,菌体表面的官能团位置未发生明显变化,推测该吸附过程是物理吸附.
Adsorption properties to different hydrophobic organic compounds by dried cells, which belonged to the fungus Ophiostoma stenoceras LLC and bacterium Pseudomonas veronii ZW, were compared, and their cell surface hydrophobicity(CSH)were analyzed. The cell surface was characterized by BET, FTIR(Fourier Transform Infrared Spectroscopy) and XPS(X-ray Photoelectron Spectroscopy). Results showed that the specific surface area of Ophiostoma sp. LLC and Pseudomonas sp. ZW was 15.8m~2/g and 11.57m~2/g respectively. With more mesoporous distributing on their surface, the fungal cells could adsorb organic compounds more efficiently than the bacterial cells. The CSH of the fungus LLC was higher than that of the bacterium ZW when they were at the same growth stage. The CSH of fungal and bacterial cells increased differently during the biodegradation ofα-pinene. After being dried, the fungus LLC cells could adsorb different hydrophobic organic compounds followed this order: ethyl acetate > α-pinene > n-hexane, suggesting that low hydrophobic organic compounds could be more easily adsorbed. XPS and FTIR analysis also indicated that the position and the number of functional groups on the surface of the fungal cells did not change obviously before and after the adsorption of organic compounds. These results suggested that the adsorption was a physical process.
Adsorption properties to different hydrophobic organic compounds by dried cells, which belonged to the fungus Ophiostoma stenoceras LLC and bacterium Pseudomonas veronii ZW, were compared, and their cell surface hydrophobicity(CSH)were analyzed. The cell surface was characterized by BET, FTIR(Fourier Transform Infrared Spectroscopy) and XPS(X-ray Photoelectron Spectroscopy). Results showed that the specific surface area of Ophiostoma sp. LLC and Pseudomonas sp. ZW was 15.8m~2/g and 11.57m~2/g respectively. With more mesoporous distributing on their surface, the fungal cells could adsorb organic compounds more efficiently than the bacterial cells. The CSH of the fungus LLC was higher than that of the bacterium ZW when they were at the same growth stage. The CSH of fungal and bacterial cells increased differently during the biodegradation ofα-pinene. After being dried, the fungus LLC cells could adsorb different hydrophobic organic compounds followed this order: ethyl acetate > α-pinene > n-hexane, suggesting that low hydrophobic organic compounds could be more easily adsorbed. XPS and FTIR analysis also indicated that the position and the number of functional groups on the surface of the fungal cells did not change obviously before and after the adsorption of organic compounds. These results suggested that the adsorption was a physical process.
引文
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[32]Jin Y,Veiga M C,Kennes C.Performance optimization of the fungal biodegradation ofα-pinene in gas-phase biofilter[J].Process Biochemistry,2006,41(8):1722-1728.
[2]Zhang S H,You J P,Kennes C,et al.Current advances of VOCs degradation by bioelectrochemical systems:A review[J].Chemical Engineering Journal,2018,334:2625-2637.
[3]Cheng Y,He H J,Yang C P,et al.Challenges and solutions for biofiltration of hydrophobic volatile organic compounds[J].Biotechnology Advances,2016,34(6):1091-1102.
[4]Delhoménie M C,Heitz M.Biofiltration of air:A Review[J].Critical Reviews in Biotechnology,2005,25:53-72.
[5]Oliver J P,Schilling J S.Capture of methane by fungi:Evidence from laboratory-scale biofilter and chromatographic isotherm studies[J].Transactions of the Asae American Society of Agricultural Engineers,2018,59(6):1791-1801.
[6]Furuno S,Remer R,Chatzinotas A,et al.Use of mycelia as paths for the isolation of contaminant-degrading bacteria from soil[J].Microbial Biotechnology,2012,5(1):142-148.
[7]Dorado A D,Baquerizo G,Maestre J P,et al.Modeling of a bacterial and fungal biofilter applied to toluene abatement:Kinetic parameters estimation and model validation[J].Chemical Engineering Journal,2013,140(1):52-61.
[8]Marshall K C,Cruickshank R H.Cell surface hydrophobicity and the orientation of certain bacteria at interfaces[J].Archiv Fur Mikrobiologie,1973,91(1):29.
[9]曲杰,王海胜,史延华,等.氯氰菊酯降解菌株L12的分离鉴定及降解特性[J].微生物学报,2011,51(4):510-517.Qu J,Wang H S,Shi Y H,et al.Isolation,Identification and degradation characteristics of cypermethrin degrading Strain L12[J].Journal of Microbiology,2011,51(4):510-517.
[10]Hamada T,Maeda Y,Matsuda H.Effect of cell-surface hydrophobicity on bacterial conversion of water-immiscible chemicals in two-liquid-phase culture systems[J].Journal of Bioscience&Bioengineering,2009,108(2):116-120.
[11]赵晴,张甲耀,陈兰洲,等.疏水性石油烃降解菌细胞表面疏水性及降解特性[J].环境科学,2005,26(5):132-136.Zhao Q,Zhang J Y,Chen L Z,et al.[Cell-surface hydrophobicity and degradation characteristics of hydrophobic hydrocarbon degrading bacteria][J].Environmental Science,2005,26(5):132-136.
[12]Stringfellow W T,Alvarez-Cohen L.Evaluating the relationship between the sorption of PAHs to bacterial biomass and biodegradation[J].Water Research,1999,33(11):2535-2544.
[13]顾海萍.Massilia sp.WF1和Phanerochaete chrysosporium对菲降解、吸附机理的研究[D].杭州:浙江大学,2016:51-54.Gu H P.Biodegradation and bioaorption mechanisms of phenanthrene by Massilia sp.WF1 and Phanerochaete chrysosporium[D].Hangzhou:Zhejiang University,2016:51-54.
[14]张向东,刘祁涛,张士国.人工神经网络法预测有机物物性的研究1.亨利定律常数和水中溶解度[J].辽宁大学学报(自然科学版),1995,22(2):38-41.Zhang X D,Liu Q T,Zhang S G.Prediction of organic matter properties by artificial neural network method 1.Henry's law constant and solubility in water[J].Journal of Liaoning University(Natural Science Edition),1995,22(2):38-41.
[15]Cheng Z W,Sun P F,Jiang Y F,et al.Kinetic analysis and bacterium metabolization ofα-pinene by a novel identified Pseudomonas sp.strain[J].Journal of Environmental Science(English),2012,24(10):1806-1815.
[16]Vergara-Fernández A,Haaren B V,Revah S.Phase partition of gaseous hexane and surface hydrophobicity of Fusarium solani when grown in liquid and solid media with hexanol and hexane[J].Biotechnology Letters,2006,28(24):2011-2017.
[17]Jiang L Y,Li S,Cheng Z W,et al.Treatment of 1,2-dichloroethane and n-hexane in a combined system of non‐thermal plasma catalysis reactor coupled with a biotrickling filter[J].Journal of Chemical Technology&Biotechnology,2017,93:127-137.
[18]Rosenberg M,Kjelleberg S.Hydrophobic Interactions:Role in Bacterial Adhesion[M].1986.
[19]宋燕,乔文明,尹圣昊,等.不同结构活性炭对甲苯的吸附性能[J].新型炭材料,2005,20(4):294-298.Song Y,Qiao W M,Yin S H,et al.Toluene adsorption on various activated carbons with different pore structures[J].New carbon material,2005,20(4):294-298.
[20]Kaczorek E,Urbanowicz M,Olszanowski A.The influence of surfactants on cell surface properties of Aeromonas hydrophila during diesel oil biodegradation[J].Colloids&Surfaces B Biointerfaces,2010,81(1):363-368.
[21]Watanabe K,Miyashita M,Harayama S.Starvation improves survival of bacteria introduced into activated sludge[J].Applied&Environmental Microbiology,2000,66(9):3905-3910.
[22]Mehmannavaz R,Prasher S O,Ahmad D.Cell surface properties of rhizobial strains isolated from soils contaminated with hydrocarbons:hydrophobicity and adhesion to sandy soil[J].Process Biochemistry,2001,36(7):683-688.
[23]Song C,Sun X F,Xing S F,et al.Characterization of the interactions between tetracycline antibiotics and microbial extracellular polymeric substances with spectroscopic approaches[J].Environmental science and pollution research international,2014,21(3):1786-1795.
[24]Yuan S J,Sun M,Sheng G P,et al.Identification of key constituents and structure of the extracellular polymeric substances excreted by Bradyrhizobium japonicumBurkholderi TF10for their flocculation capacity[J].Environmental Science&Technology,2011,45(3):1152-1157.
[25]Miguez C B,Beveridge T J,Ingram J M.Lipopolysaccharide changes and cytoplasmic polyphosphate granule accumulation in Pseudomonas aeruginosa during growth on hexadecane[J].Canadian Journal of Microbiology,1986,32(3):248-253.
[26]Park K M,So J S.Altered cell surface hydrophobicity of lipopolysaccharide-deficient mutant of Bradyrhizobium japonicum[J].Journal of Microbiological Methods,2000,41(3):219-226.
[27]Chen B L,Wang Y S,Hu D F.Biosorption and biodegradation of polycyclic aromatic hydrocarbons in aqueous solutions by a consortium of white-rot fungi[J].Journal of Hazardous Materials,2010,179(1):845-851.
[28]Das S K,Ghosh P,Ghosh I,et al.Adsorption of rhodamine B on Rhizopus oryzae:role of functional groups and cell wall components[J].Colloids&Surfaces B Biointerfaces,2008,65(1):30-34.
[29]Qh G,Pikpggtkpi P.The past,present,and future trends of biosorption[J].Biotechnology and Bioproccess Engineering,2010,15(1):86-102.
[30]丁洁.白腐真菌对多环芳烃的生物吸附与生物降解及其修复作用[D].杭州:浙江大学,2012:34-35.Ding J.Biosorption,biodegradation and repair of polycyclic aromatic hydrocarbons by White Rot fungi[D].Hangzhou:Zhejiang University,2012:34-35.
[31]马佳林,聂小琴,董发勤,等.三种微生物对铀的吸附行为研究[J].中国环境科学,2015,(3):825-832.Ma J L,Nie X Q,Dong F Q,et al.Adsorption behavior of three kinds of microorganisms on uranium[J].Chinese Environmental Science,2015,(3):825-832.
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