胃癌组织中长链非编码RNA AK093987表达及其临床意义
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摘要
目的探讨胃癌组织中长链非编码RNA(lncRNA)AK093987表达及其临床意义。方法将2016年4月至2018年4月本院收治的152例胃癌患者纳入研究对象,应用RT-PCR技术检测患者胃癌组织及癌旁组织中lncRNA AK093987的表达,并分析其与患者临床病理特征的相关性。结果 RT-PCR检测结果显示,胃癌组织中lncRNA AK093987相对表达量(7.24±0.88)明显高于癌旁组织(1.07±0.26),两组相比差异有统计学意义(P<0.05)。胃癌组织中lncRNA AK093987表达与肿瘤直径、分化程度、TNM分期、淋巴结转移、深层浸润、远端转移密切相关,差异有统计学意义(P<0.05),而与性别、年龄、肿瘤位置无关,差异无统计学意义(P>0.05)。结论 lncRNA AK093987在胃癌组织中呈高表达,其表达水平升高与胃癌的恶性程度呈正相关,提示lncRNA AK093987可能与胃癌的发生、发展有关。
Objective To investigate the expression and clinical significance of long-chain non-coding RNA(lncRNA)AK093987 in gastric cancer tissues. Methods A total of 152 patients with gastric cancer admitted to our hospital from April 2016 to April 2018 were included in the study. RT-PCR was used to detect the expression of lncRNA AK093987 in gastric cancer tissues and adjacent tissues. The correlation with the clinicopathological features of the patients was analyzed.Results The results of RT-PCR showed that the relative expression of lncRNA AK093987 in gastric cancer tissues(7.24±0.88) was significantly higher than that in adjacent tissues(1.07±0.26). The difference between the two groups was statistically significant(P<0.05). The expression of lncRNA AK093987 in gastric cancer tissues was closely related to tumor diameter,differentiation degree,TNM stage,lymph node metastasis,deep infiltration and distant metastasis(P<0.05),but not related to gender,age and tumor location(P>0.05). Conclusion lncRNA AK093987 was highly expressed in gastric cancer tissues,and its expression level was positively correlated with the malignant degree of gastric cancer,suggesting that lncRNA AK093987 might be related to the occurrence and development of gastric cancer.
Objective To investigate the expression and clinical significance of long-chain non-coding RNA(lncRNA)AK093987 in gastric cancer tissues. Methods A total of 152 patients with gastric cancer admitted to our hospital from April 2016 to April 2018 were included in the study. RT-PCR was used to detect the expression of lncRNA AK093987 in gastric cancer tissues and adjacent tissues. The correlation with the clinicopathological features of the patients was analyzed.Results The results of RT-PCR showed that the relative expression of lncRNA AK093987 in gastric cancer tissues(7.24±0.88) was significantly higher than that in adjacent tissues(1.07±0.26). The difference between the two groups was statistically significant(P<0.05). The expression of lncRNA AK093987 in gastric cancer tissues was closely related to tumor diameter,differentiation degree,TNM stage,lymph node metastasis,deep infiltration and distant metastasis(P<0.05),but not related to gender,age and tumor location(P>0.05). Conclusion lncRNA AK093987 was highly expressed in gastric cancer tissues,and its expression level was positively correlated with the malignant degree of gastric cancer,suggesting that lncRNA AK093987 might be related to the occurrence and development of gastric cancer.
引文
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[16]李春梅,王彪,邹博,等.转录调控因子Nkx2.1和lncRNANANCI在支气管肺发育不良中的表达及意义[J].热带医学杂志,2017,17(11):1441-1443,1453,1573.
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[2]符涛,季加孚.胃癌诊疗的热点和问题[J].中国肿瘤临床,2016,43(1):2-5.
[3]Amieva M,Peek RM Jr.Pathobiology of Helicobacter pyloriinduced gastric cancer[J].Gastroenterology,2016,150(1):64-78.
[4]Bhan A,Soleimani M,Mandal SS.Long noncoding RNA and cancer:A new paradigm[J].Cancer Res,2017,77(15):3965-3981.
[5]Du Y,Xia W,Zhang J,et al.Comprehensive analysis of long noncoding RNA-mRNA co-expression patterns in thyroid cancer[J].Mol Biosyst,2017,13(10):2107.
[6]Kotake Y,Goto T,Naemura M,et al.Long noncoding RNAPANDA positively regulates proliferation of osteosarcoma cells[J].Anticancer Res,2017,37(1):81-85.
[7]胡星,邬四明,王昕,等.亚洲人群NQO1 C609T基因多态性与胃癌易感性的Meta分析[J].中国热带医学,2016,16(6):608-611.
[8]国家消化系统疾病临床医学研究中心,中华医学会消化内镜学分会,中华医学会健康管理学分会,等.中国早期胃癌筛查流程专家共识意见(草案)(2017年,上海)[J].胃肠病学,2018,23(2):92-97.
[9]吴军,徐美珍.胃癌患者血清CK-18、CA72-4、CEA的临床意义[J].热带医学杂志,2016,16(12):1533-1535.
[10]Cui Y,Zhang F,Zhu C,et al.Upregulated lncRNA SNHG1 contributes to progression of non-small cell lung cancer through inhibition of miR-101-3p and activation of Wnt/β-catenin signaling pathway[J].Oncotarget,2017,8(11):17785-17794.
[11]Peng WX,Koirala P,Mo YY.LncRNA-mediated regulation of cell signaling in cancer[J].Oncogene,2017,36(41):5661-5667.
[12]Zhang K,Shi H,Xi H,et al.Genome-Wide lncRNA microarray profiling identifies novel circulating lncRNAs for detection of gastric cancer[J].Theranostics,2017,7(1):213-227.
[13]王月月,杨清玲,陈昌杰.长链非编码RNA与乳腺癌耐药研究[J].分子诊断与治疗杂志,2017,9(6):426-429,436.
[14]Zheng P,Chen L,Yuan X,et al.Exosomal transfer of tumorassociated macrophage-derived miR-21 confers cisplatin resistance in gastric cancer cells[J].J Exp Clin Cancer Res,2017,36(1):53.
[15]Zhang M,Li CC,Li F,et al.Estrogen promotes hepatic synthesis of long-chain polyunsaturated fatty acids by regulating ELOVL5 at post-transcriptional level in laying hens[J].Int JMol Sci,2017,18(7).pii:E1405.
[16]李春梅,王彪,邹博,等.转录调控因子Nkx2.1和lncRNANANCI在支气管肺发育不良中的表达及意义[J].热带医学杂志,2017,17(11):1441-1443,1453,1573.
[17]Liu Q,Guo X,Que S,et al.LncRNA RSU1P2 contributes to tumorigenesis by acting as a ce RNA against let-7a in cervical cancer cells[J].Oncotarget,2017,8(27):43768-43781.
[18]Zhang M,Wu WB,Wang ZW,et al.lncRNA NEAT1 is closely related with progression of breast cancer via promoting proliferation and EMT[J].Eur Rev Med Pharmacol Sci,2017,21(5):1020-1026.
[19]Hao NB,He YF,Li XQ,et al.The role of miRNA and lncRNAin gastric cancer[J].Oncotarget,2017,8(46):81572-81582.
[20]Shao Y,Li J,Lu R,et al.Global circular RNA expression profile of human gastric cancer and its clinical significance[J].Cancer Med,2017,6(6):1173-1180.