Wnt7a/PCP信号通路在肾衰营养胶囊改善CRF大鼠骨骼肌萎缩中的作用
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摘要
目的探讨Wnt7a/PCP信号通路在肾衰营养胶囊改善CRF大鼠骨骼肌萎缩中的作用及机制。方法将大鼠随机分为正常组、模型组、中药组和开同组,采用切除5/6肾并予4%酪蛋白饲料喂养制作CRF营养不良大鼠模型。采用免疫组化法检测TA中Myogenin和Myo D的表达;采用Western blot检测Wnt7a/PCP信号通路中的蛋白表达。结果肾衰营养胶囊增加CRF大鼠体重和骨骼肌质量,增加TA中Myogenin和Myo D蛋白的表达,促进骨骼肌组织Wnt7a/PCP信号通路中蛋白表达上调。结论肾衰营养胶囊具有改善CRF模型大鼠骨骼肌萎缩的作用,其机制可能与上调骨骼肌Wnt7a/PCP信号通路、促进MSC增殖分化相关。
引文
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[3]Le Grand F,Jones A E,Seale V,et al.Wnt7a activates the planar cell polarity pathway to drive the symmetric expansion of satellite stem cells[J].Cell Stem Cell,2009,4(6):535.
[4]Ikizler T A,Cano N J,Franch H,et al.Prevention and treatment of protein energy wasting in chronic kidney disease patients:a consensus statement by the International Society of Renal Nutrition and Metabolism[J].Kidney Int,2013,84(6):1096.
[5]魏连波,张颖,汪南海.肾衰养真颗粒治疗慢性肾脏病4、5期并发营养不良的临床观察[J].中国中西医结合肾病杂志,2009,10(8):696.
[6]魏连波,邓聪,陈杰彬,等.肾衰养真胶囊对5/6肾切除慢性肾衰营养不良大鼠营养状态的影响[J].中药材,2008,31(9):1391.
[7]王均,贾弘.鸡骨骼肌发育过程生肌素的表达[J].生物化学杂志,1996,12(5):507.
[8]Le Grand F,Jones A E,Seale V,et al.Wnt7a activates the planar cell polarity pathway to drive the symmetric expansion of satellite stem cells[J].Cell Stem Cell,2009,4(6):535.