芎姜超临界CO_2提取物透皮凝胶的经皮渗透研究
|
摘要
目的:研究川芎、炮姜超临界CO2提取物透皮凝胶(简称芎姜超临界CO2提取物凝胶)的经皮渗透性。方法:通过透皮扩散仪测定芎姜超临界CO2提取物凝胶经离体大鼠皮肤的渗透性,分别以藁本内酯、6-姜酚为指标成分,高效液相色谱(HPLC)法测定药物含量求算累积渗透量(Q)与稳态透皮速率(JS)。结果:芎姜超临界CO2提取物凝胶中藁本内酯和6-姜酚均能有效经皮渗透,Js分别为(47.57±3.77)、(8.29±1.47)μg/(cm2·h),14 h平均累积渗透百分数分别为(26.99±1.89)%和(14.40±1.18)%。结论:芎姜超临界CO2提取物凝胶具有良好的经皮渗透性,经皮给药有望作为芎姜超临界CO2提取物的一种新给药途径。
OBJECTIVE:To study the transdermal permeation of hydrogel containing CO2-SFE of Ligusticum chuanxiong and Zingiber officinale(called Hydrogel containing Xiongjiang CO2-SFE for short). METHODS:The transdermal permeation of Hydrogel containing Xiongjiang CO2-SFE was determined by transdermal diffusion device,with ligustilide and 6-shogaol as indicators. Accumulative transdermal quantity and static percutaneous rate of drugs were determined by HPLC. RESULTS:The results showed that ligustilide and 6-shogaol could both permeate across the rat skin in vitro;static percutaneous rate of them were(47.57±3.77)μg/(cm-2·h)and(8.29±1.47)μg/(cm2·h);14 h average accumulative permeation percentages were(26.99±1.89)% and(14.40±1.18)%,respectively. CONCLUSIONS:The results show good transdermal permeation of Hydrogel containing Xiongjiang CO2-SFE. The percutanous administration is a potential route of administration for Xiongjiang CO2-SFE.
OBJECTIVE:To study the transdermal permeation of hydrogel containing CO2-SFE of Ligusticum chuanxiong and Zingiber officinale(called Hydrogel containing Xiongjiang CO2-SFE for short). METHODS:The transdermal permeation of Hydrogel containing Xiongjiang CO2-SFE was determined by transdermal diffusion device,with ligustilide and 6-shogaol as indicators. Accumulative transdermal quantity and static percutaneous rate of drugs were determined by HPLC. RESULTS:The results showed that ligustilide and 6-shogaol could both permeate across the rat skin in vitro;static percutaneous rate of them were(47.57±3.77)μg/(cm-2·h)and(8.29±1.47)μg/(cm2·h);14 h average accumulative permeation percentages were(26.99±1.89)% and(14.40±1.18)%,respectively. CONCLUSIONS:The results show good transdermal permeation of Hydrogel containing Xiongjiang CO2-SFE. The percutanous administration is a potential route of administration for Xiongjiang CO2-SFE.
引文
[1]李晓勤,张瑛,孙江川,等.剖宫产与自然分娩子宫复旧情况的比较[J].重庆医科大学学报,2009,34(5):640.
[2]左燕,陈丽宏,谢人明.试论中成药子宫复旧促进剂的概念、分类及临床应用[J].中国医院药学杂志,2011,31(12):1014.
[3]张帆,吴成,林珊.温经活血扶正法对剖宫产术后子宫复旧及血液流变学的影响[J].中国实验方剂学杂志,2013,19(3):282.
[4]黄益平.产后子宫复旧不全的中医药防治进展[J].河南中医,2012,32(1):124.
[5]王明军,叶定江,朱荃.傅氏生化汤缩宫效应的正交设计分析[J].成都中医药大学学报,2000,23(4):39.
[6]钦富华,夏晓静,董宇,等.UV-Vis测定芎姜复方CO2超临界提取物中总内酯和总萜类的含量[J].中南药学,2013,11(3):222.
[7]吴平丽,刘雯,张继全,等.川芎中洋川芎内酯A和Z-蒿本内酯的HPLC法测定[J].中国医药工业杂志,2010,41(4):290.
[8]文红梅,彭国平,朱国元,等.HPLC内标法同时测定坤血安软胶囊中藁本内酯和6-姜酚的含量[J].中成药,2007,29(3):371.
[9]刘晓昱,饶跃峰,梁文权.炔雌醇醇质体凝胶的经皮渗透研究[J].中国药学杂志,2006,41(4):284.
[10]王小芳,郭建博,徐晖,等.川芎和香附超临界CO2提取物及其制剂的体外经皮透过性[J].中国药剂学杂志,2011,9(5):91.
[11]胡英,陈心舒,夏晓静.氯雷他定包合物凝胶的体外透皮研究[J].中国药房,2010,21(29):2 727.
[2]左燕,陈丽宏,谢人明.试论中成药子宫复旧促进剂的概念、分类及临床应用[J].中国医院药学杂志,2011,31(12):1014.
[3]张帆,吴成,林珊.温经活血扶正法对剖宫产术后子宫复旧及血液流变学的影响[J].中国实验方剂学杂志,2013,19(3):282.
[4]黄益平.产后子宫复旧不全的中医药防治进展[J].河南中医,2012,32(1):124.
[5]王明军,叶定江,朱荃.傅氏生化汤缩宫效应的正交设计分析[J].成都中医药大学学报,2000,23(4):39.
[6]钦富华,夏晓静,董宇,等.UV-Vis测定芎姜复方CO2超临界提取物中总内酯和总萜类的含量[J].中南药学,2013,11(3):222.
[7]吴平丽,刘雯,张继全,等.川芎中洋川芎内酯A和Z-蒿本内酯的HPLC法测定[J].中国医药工业杂志,2010,41(4):290.
[8]文红梅,彭国平,朱国元,等.HPLC内标法同时测定坤血安软胶囊中藁本内酯和6-姜酚的含量[J].中成药,2007,29(3):371.
[9]刘晓昱,饶跃峰,梁文权.炔雌醇醇质体凝胶的经皮渗透研究[J].中国药学杂志,2006,41(4):284.
[10]王小芳,郭建博,徐晖,等.川芎和香附超临界CO2提取物及其制剂的体外经皮透过性[J].中国药剂学杂志,2011,9(5):91.
[11]胡英,陈心舒,夏晓静.氯雷他定包合物凝胶的体外透皮研究[J].中国药房,2010,21(29):2 727.