甲状腺乳头状癌中Nrf2表达及与淋巴结转移的关系
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摘要
目的探讨Nrf2在甲状腺乳头状癌中的表达及与淋巴结转移的关系。方法应用免疫组化检测61例甲状腺乳头状癌中Nrf2的表达。结果 Nrf2总阳性率91.8%,其中无淋巴结转移组阳性率97.4%(38/39),有淋巴结转移组阳性率81.8%(18/22)(P<0.01);未侵犯包膜组阳性率100%(24/24),侵犯包膜组阳性率86.5%(32/37)(P<0.01);提示Nrf2在甲状腺乳头状癌中的表达与淋巴结转移及包膜侵犯呈负相关关系。结果还显示,在Nrf2阳性的病例中,未侵犯包膜与侵犯包膜组淋巴结转移率分别是16.7%(4/24)及43.8%(14/32)(P<0.05),提示尽管Nrf2阳性,侵犯包膜组明显比未侵犯包膜组淋巴结转移机会大;在侵犯包膜的病例中,Nrf2阴性及Nrf2阳性病例的淋巴结转移率分别是80%(4/5)及43.8%(14/32)(P<0.05),提示侵犯包膜即使Nrf2阴性时,淋巴结转移的可能性仍很大。结论 Nrf2与甲状腺乳头状癌侵袭转移密切相关,Nrf2阳性率降低能抑制肿瘤侵袭转移。
Objective To explore the expression of Nrf2 in human thyroid papillary carcinoma and its relationship to lymph node metastasis. Methods The 61 cases surgical specimens of human thyroid papillary carcinoma were used to detect the expression of Nrf2 by immunohistochemistry. Results The positive rate of Nrf2 in all groups was 91.8%. The positive rate of no lymph node metastasis group was 97.4%(38/39). The positive rate of lymph node metastasis group was 81.8%(18/22). There was statistically different between no lymph node metastasis group and lymph node metastasis group in positive rate(P<0.01). The positive rate of no invading capsule group was 100%(24/24). The positive rate of capsule-invaded group was 86.5%(32/37). There was statistically different between non-capsule-invaded group and capsule-invaded group in positive rate(P<0.01). The expression of Nrf2 in human thyroid papillary carcinoma was negatively correlated with lymph node metastasis and capsular invasion. The results also showed that in the case of Nrf2 positive expression, the lymph node metastasis rate of the non-capsule-invaded group and the capsule-invaded group were 16.7%(4/24) and 43.8%(14/32). There was statistical difference between non-capsule-invaded envelope group and capsule-invaded group in lymph node metastasis rate(P<0.05). It was suggested that despite Nrf2 positive expression, the capsule-invaded group had more chance of lymph node metastasis than non-capsule-invaded group. In the case of capsule invasion, the lymph node metastasis rate of Nrf2 negative expression and Nrf2 positive expression were 80%(4/5)and 43.8%(14/32). There was statistically different between Nrf2 positive expression group and Nrf2 negative expression group in lymph node metastasis rate(P<0.05). It was suggested that despite capsule invasion, Nrf2 negative expression group had more chance of lymph node metastasis than Nrf2 positive expression group. Conclusion Nrf2 is closely related to the invasion and metastasis of thyroid papillary carcinoma. Decreased expression of Nrf2 can inhibit invasion and lymph node metastasis in thyroid papillary carcinoma.
Objective To explore the expression of Nrf2 in human thyroid papillary carcinoma and its relationship to lymph node metastasis. Methods The 61 cases surgical specimens of human thyroid papillary carcinoma were used to detect the expression of Nrf2 by immunohistochemistry. Results The positive rate of Nrf2 in all groups was 91.8%. The positive rate of no lymph node metastasis group was 97.4%(38/39). The positive rate of lymph node metastasis group was 81.8%(18/22). There was statistically different between no lymph node metastasis group and lymph node metastasis group in positive rate(P<0.01). The positive rate of no invading capsule group was 100%(24/24). The positive rate of capsule-invaded group was 86.5%(32/37). There was statistically different between non-capsule-invaded group and capsule-invaded group in positive rate(P<0.01). The expression of Nrf2 in human thyroid papillary carcinoma was negatively correlated with lymph node metastasis and capsular invasion. The results also showed that in the case of Nrf2 positive expression, the lymph node metastasis rate of the non-capsule-invaded group and the capsule-invaded group were 16.7%(4/24) and 43.8%(14/32). There was statistical difference between non-capsule-invaded envelope group and capsule-invaded group in lymph node metastasis rate(P<0.05). It was suggested that despite Nrf2 positive expression, the capsule-invaded group had more chance of lymph node metastasis than non-capsule-invaded group. In the case of capsule invasion, the lymph node metastasis rate of Nrf2 negative expression and Nrf2 positive expression were 80%(4/5)and 43.8%(14/32). There was statistically different between Nrf2 positive expression group and Nrf2 negative expression group in lymph node metastasis rate(P<0.05). It was suggested that despite capsule invasion, Nrf2 negative expression group had more chance of lymph node metastasis than Nrf2 positive expression group. Conclusion Nrf2 is closely related to the invasion and metastasis of thyroid papillary carcinoma. Decreased expression of Nrf2 can inhibit invasion and lymph node metastasis in thyroid papillary carcinoma.
引文
[1] 曲真真,石建华,汪万英.甲状腺乳头状癌Nrf2和bcl-2表达的临床意义[J].蚌埠医学院学报,2009,34(11):965-968.
[2] Ishii T,Itoh K,Takahashi S,et al.Transcription factor Nrf2 coordinately regulates a group of oxidative stress-inducible genes in macrophages [J].Biol Chem,2000,275(21):16023-16029.
[3] 耿文静,单立兵,王金生,等.红系衍生的核因子2相关因子2在结节性甲状腺肿和甲状腺乳头状癌的表达及意义[J].中华肿瘤杂志,2017,39(5):367-368.
[4] 黎金颜,纪晓丹,吴又明,等.子宫内膜腺癌组织FRA和CA125的表达及意义[J].实用医学杂志,2017,33(4):594-598.
[5] 沈丹华,谢俊玲,张银丽,等.叶酸受体α在卵巢上皮性肿瘤中的表达[J].中华病理学杂志,2010,39(11):747-751.
[6] 倪静,秦丽娟,吴拥军,等.肺癌组织中Nrf2、Keap1和NQO1蛋白的表达[J].郑州大学学报(医学版),2012,47(4):456-459.
[7] Lau A,Villeneuve NF,Sun Z,et al.Dual roles of Nrf2 in cancer[J].Pharmacol Res,2008,58(5/6):262-270.
[8] Kim YJ,Ahn JY,Liang P,et al.Human prx1 gene is a target of Nrf2 and is up-regulated by hypoxia/reoxygenation:implication to tumor biology [J].Cancer Res,2007,67(2):546-554.
[9] Yanagawa T,Omura K,Harada H,et al.Heme oxygenase-1 expression predicte cervical lymph node metastasis of tongue squamous cell carcinomas [J].Oral Oncol,2004,40(1):21-27.
[10] Nioi P,Nguyen T.A mutation of Keap1 found in breast cancer impairs its ability to repress Nrf2 activity [J].Biochem Biophys Res Commun,2007,362(4):816-821.
[11] 张钧,孔德友,张舰,等.RNA干扰Nrf2表达对食管癌生物学行为的影响[J].临床肿瘤学杂志,2014,19(12):1057-1061.
[12] 庄依泽,梁宇,殷红专,等.NRF2 /HO-1因子与结直肠肿瘤TNM分期的相关性研究[J].现代肿瘤医学,2015,23(16):2318-2322.
[13] 李晴,徐畅,刘强.核因子E2相关因子2及其在肿瘤防治中的作用[J].国际肿瘤学杂志,2015,42(8):608-611.
[2] Ishii T,Itoh K,Takahashi S,et al.Transcription factor Nrf2 coordinately regulates a group of oxidative stress-inducible genes in macrophages [J].Biol Chem,2000,275(21):16023-16029.
[3] 耿文静,单立兵,王金生,等.红系衍生的核因子2相关因子2在结节性甲状腺肿和甲状腺乳头状癌的表达及意义[J].中华肿瘤杂志,2017,39(5):367-368.
[4] 黎金颜,纪晓丹,吴又明,等.子宫内膜腺癌组织FRA和CA125的表达及意义[J].实用医学杂志,2017,33(4):594-598.
[5] 沈丹华,谢俊玲,张银丽,等.叶酸受体α在卵巢上皮性肿瘤中的表达[J].中华病理学杂志,2010,39(11):747-751.
[6] 倪静,秦丽娟,吴拥军,等.肺癌组织中Nrf2、Keap1和NQO1蛋白的表达[J].郑州大学学报(医学版),2012,47(4):456-459.
[7] Lau A,Villeneuve NF,Sun Z,et al.Dual roles of Nrf2 in cancer[J].Pharmacol Res,2008,58(5/6):262-270.
[8] Kim YJ,Ahn JY,Liang P,et al.Human prx1 gene is a target of Nrf2 and is up-regulated by hypoxia/reoxygenation:implication to tumor biology [J].Cancer Res,2007,67(2):546-554.
[9] Yanagawa T,Omura K,Harada H,et al.Heme oxygenase-1 expression predicte cervical lymph node metastasis of tongue squamous cell carcinomas [J].Oral Oncol,2004,40(1):21-27.
[10] Nioi P,Nguyen T.A mutation of Keap1 found in breast cancer impairs its ability to repress Nrf2 activity [J].Biochem Biophys Res Commun,2007,362(4):816-821.
[11] 张钧,孔德友,张舰,等.RNA干扰Nrf2表达对食管癌生物学行为的影响[J].临床肿瘤学杂志,2014,19(12):1057-1061.
[12] 庄依泽,梁宇,殷红专,等.NRF2 /HO-1因子与结直肠肿瘤TNM分期的相关性研究[J].现代肿瘤医学,2015,23(16):2318-2322.
[13] 李晴,徐畅,刘强.核因子E2相关因子2及其在肿瘤防治中的作用[J].国际肿瘤学杂志,2015,42(8):608-611.