芜湖产地金银花的质量分析
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摘要
利用HPLC方法测定芜湖产金银花中的绿原酸的含量。采用HPLC法,色谱柱为Agllent HC-C18(5μm,4.6×250mm);流动相为乙腈-0.4%磷酸溶液,洗脱;波长327nm,柱温30℃。线性良好(R=0.9997),精密度良好(0.11%),回收率良好(平均回收率为103.15%,RSD为1.97%)。结果表明,芜湖丫山产金银花中有效成分绿原酸高于药典规定,平均含量为2.1%。
Determination of Chlorogenic acid in Flos Lonicerafrom Wuhu by HPLC.Methods:A HPLC method was established with Agllent HC-C18(5μm,4.6×250 mm)and the mobile phase was acetonitrile-0.4% phosphoric acid solution.The wavelength was 327 nm and the column temperature was 30℃.The linear calibration curve was good(R=0.9997),the precision was good(0.11 %),the average recoveries were good(the average recovery rate was103.15%,RSD was 1.97%).The results were that the chlorogenic acid in Lonicera japonica Thunb in Wuhu was higher than that in Ch.P.,and the average content was 2.1%.
Determination of Chlorogenic acid in Flos Lonicerafrom Wuhu by HPLC.Methods:A HPLC method was established with Agllent HC-C18(5μm,4.6×250 mm)and the mobile phase was acetonitrile-0.4% phosphoric acid solution.The wavelength was 327 nm and the column temperature was 30℃.The linear calibration curve was good(R=0.9997),the precision was good(0.11 %),the average recoveries were good(the average recovery rate was103.15%,RSD was 1.97%).The results were that the chlorogenic acid in Lonicera japonica Thunb in Wuhu was higher than that in Ch.P.,and the average content was 2.1%.
引文
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[2]国家药典委员会.中国药典(一部)[S].北京:中国医药科技出版社,2015:221.
[3]邢志霞,韩永成,刘伟,等.UHUPLC和HPLC法对比测定不同产地金银花药材中绿原酸的含量[J].辽宁中医杂志,2014,41(05):992-993.
[4]冯煜.对不同产地金银花原药材质量的研究[J].现代中药研究与实践,2010,24(06):34-35.
[5]李淼,王永香,孟谨,等.HPLC法测定金银花中绿原酸等8种成分的量[J].中草药,2014,45(07):1006-1010.
[6]辛华,丰杰,程若敏,等.HPLC测定不同产地金银花中绿原酸和木犀草苷[J].中国实验方剂学杂志,2011,17(02):60-63.
[7]解世全,王帅,孟宪生,等.不同产地金银花质量评价[J].中国实验方剂学杂志,2016,22(05):80-83.
[8]李斌.山东产金银花药材现代质量的应用研究[D].济宁:山东大学,2011:60-67.
[9]刘敏彦,高淑丽,刘丽华,等.HPLC法同时测定不同产地金银花和山银花中6种有机酸成分[J].中草药,2013,36(02):196-198.
[10]韩永成,刘伟,陈宁,等.不同产地金银花药材的UPLC指纹图谱分析[J].中国实验方剂学杂志,2014,20(02):67-69.
[11]赵军峰,马丽苹,张彬,等.金银花HPLC指纹图谱的建立[J].中国食品学报,2015,15(02):178-184.